Physiology Commons^™

Hsp90 Inhibition Suppresses Nf-Κb Transcriptional Activation Via Sirt-2 In Human Lung Microvascular Endothelial Cells, Gagan S. Thangjam, Charalampos Birmpas, Nektarios Barabutis, Betsy W. Gregory, Mary Ann Clemens, Joseph R. Newton, David Fulton, John D. Catravas

Bioelectrics Publications

The ability of anti-heat shock protein 90 (Hsp90) drugs to attenuate NF-κB-mediated transcription is the major basis for their anti-inflammatory properties. While the molecular mechanisms underlying this effect are not clear, they appear to be distinct in human endothelial cells. We now show for the first time that type 2 sirtuin (Sirt-2) histone deacetylase binds human NF-κB target gene promoter and prevents the recruitment of NF-κB proteins and subsequent assembly of RNA polymerase II complex in human lung microvascular endothelial cells. Hsp90 inhibitors stabilize the Sirt-2/promoter interaction and impose a “transcriptional block,” which is reversed by either inhibition or downregulation …

Total And Regional Bmd Comparison Of Collegiate Male And Female Athletes, Michael G. Robinson 6978741

Williams Honors College, Honors Research Projects

Total and Regional BMD Comparison of Collegiate Male and Female Athletes

Abstract

INTRODUCTION: Physiological and biomechanical demands of different sports have varying effects on the athletes that play them. For example, previous studies have shown that runners and swimmers can have lower bone mineral density (BMD) than athletes in ball sports or inactive controls. PURPOSE: To quantify and compare BMD in athletes to better understand how certain sport specific exercise promotes bone acquisition. METHODS: Fifty one (N=51) NCAA Division I athletes (18-25 yrs) had a whole body DXA scan performed to quantify total body BMD and segmental BMD. Fifteen (n=15) …

Factors Affecting Injury History And Their Overall Impact On Athletic Performance In Distance Runners, Trevor Norris

Williams Honors College, Honors Research Projects

Distance running is a very precise mechanism of many intricate movements and abilities of the body. The amount of miles that a distance runner must run puts a lot of very intense stresses on one’s body. These stresses often appear at the bone level and lead to many injuries such as stress reactions and stress fractures but also may play important roles in tissue damages such as strains and pulls. In this study, we used division one college distance runners and examined many intricacies between their level of fitness and overall composition. These include looking at imbalances, dominant traits, and …

A Flexible Pet-Based Wearable Sensor For Arterial Pulse Waveform Measurement, Dan Wang, Dean Krusienski, Zhili Hao

Mechanical & Aerospace Engineering Faculty Publications

In light of the need of health monitoring, the paper presents a flexible polyethylene terephthalate (PET)-based wearable sensor for arterial pulse waveform measurement. The sensor encompasses a polydimethylsiloxane (PDMS) microstructure embedded with an electrolyte-enabled 5×1 transducer array, which spans 6mm and has a spatial resolution of 1.5mm. A pulse signal exerts a deflection on the microstructure and is recorded as a resistance change by a transducer at the site of the pulse. An untrained individual can easily align the sensor on a targeted artery with a negligible margin and then acquire the arterial pulse waveform continuously and non-invasively. This sensor …

Selective Induced Altered Coccidians To Immunize And Prevent Enteritis, Helieh S. Oz

Physiology Faculty Publications

Microbiomic flora in digestive tract is pivotal to the state of our health and disease. Antibiotics affect GI, control composition of microbiome, and shift equilibrium from health into disease status. Coccidiosis causes gastrointestinal inflammation. Antibiotic additives contaminate animal products and enter food chain, consumed by humans with possible allergic, antibiotic resistance and enigmatic side effects. Purposed study induced nonpathogenic, immunogenic organisms to protect against disease and abolish antibiotics’ use in food animals and side effects in man. Diverse species of Coccidia were used as model. Immature organisms were treated with serial purification procedure prior to developmental stages to obtain altered …

Gender Differences In Vo2 And Hr Kinetics At The Onset Of Moderate And Heavy Exercise Intensity In Adolescents, Nicola Lai, Alessandro Martis, Alfredo Belfiori, Fatima Tolentino-Silva, Melita M. Nasca, James Strainic, Marco E. Cabrera

Electrical & Computer Engineering Faculty Publications

The majority of the studies on VO₂ kinetics in pediatric populations investigated gender differences in prepubertal children during submaximal intensity exercise, but studies are lacking in adolescents. The purpose of this study was to test the hypothesis that gender differences exist in the VO₂ and heart rate (HR) kinetic responses to moderate (M) and heavy (H) intensity exercise in adolescents. Twenty-one healthy African-American adolescents (9 males, 15.8 ± 1.1 year; 12 females, 15.7 ± 1 year) performed constant work load exercise on a cycle ergometer at M and H. The VO₂ kinetics of the male group was …

Muscle Metaboreflex And Arterial Baroreflex: Action, Interaction And Altered Control In Heart Failure, Jasdeep Kaur

Wayne State University Dissertations

Stimulation of skeletal muscle afferents by metabolites that accumulate in the exercising muscle elicits a large pressor response, termed the muscle metaboreflex. Muscle metaboreflex activation during submaximal exercise induces large increases in arterial pressure, cardiac output, heart rate and ventricular contractility however, the vascular responses have varied in previous studies. We addressed three specific questions: 1) what are the mechanism(s) regulating the non-ischemic vasculature during muscle metaboreflex activation in normal subjects, 2) whether muscle metaboreflex activation vasoconstricts the ischemic active muscle from which this reflex originates and if this vasoconstriction is exaggerated in heart failure and 3) how do the …

Role Of Secretory Granule Heterogeneity In Calcium-Triggered Exocytosis, Tejeshwar Rao

Wayne State University Dissertations

The sympathetic nervous system is activated by a variety of threats to organismal homeostasis. The adrenomedullary chromaffin cell is the core effector of sympathetic activity in the peripheral nervous system. By design, the chromaffin cell secretory response is mutable so that release can be rapidly tuned to drive context-dependent changes in physiological function. However, the mechanisms by which this tuning is achieved with such high temporal fidelity and context specificity remain unclear. This represents a major gap in our understanding of the sympatho-adrenal system since it is known to modify the function of nearly every organ system in the body. …

Cross Sensitization Of Depressive-Like Behavior Through Two Depression Related Paradigms: Maternal Separation And Its Effect On The Forced Swim Test In The Guinea Pig, Amanda Danielle Schreibeis

Browse all Theses and Dissertations

Separation and Its Effect on the Forced Swim Test In the Guinea Pig Early-life stress such as parental neglect, absence, or abandonment, has been hypothesized to increase the susceptibility for developing depression later in life via sensitization of stress-responsive physiological systems (e.g., pro-inflammatory cytokines, hypothalamic-pituitary-adrenal axis). Guinea pigs offer a potential model, but study has been limited to behavioral observations obtained during maternal separation tests. This thesis examined the generalization of this response by asking whether it would cross-sensitize to behavior in another depressive-related paradigm, the forced swim test. In three experiments, pups underwent three forced swim trials, in shallower …

Sk Channel Clustering In Sod1-G93a Motoneurons, Saihari Shekar Dukkipati

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Amyotrophic lateral sclerosis (ALS) is a devastating neuromuscular disease that currently has no cure and extremely limited treatment options. The specific mechanisms that underlie motoneuron degeneration and death, which are classical features of this disease, are mostly unknown. This thesis tests the hypothesis that small-conductance calcium-activated potassium channels (SK) may be downregulated in ALS motoneurons, as suggested by computational modelling. SK channel expression was measured in spinal alpha-motoneuron cell bodies or somata of wildtype (WT) and mutant (mt) SOD1-G93A mice, a transgenic animal model of ALS. Quantitative immunohistochemical analysis of the developmental expression of SK channel isoforms SK2 and SK3 …

Social Buffering By Unfamiliar Adult Males In Preweaning Guinea Pigs (Cavia Pocellus): The Effects On Hpa Activity And Fos Induction In The Medial Prefrontal Cortex, Withayapon Watanasriyakul

Browse all Theses and Dissertations

Social buffering, a phenomenon in which the presence of a social partner can reduce stress responses, is often most effective between strongly attached partners. Our laboratory previously found a surprising buffering effect of the hypothalamic-pituitary-adrenal (HPA) response in preweaning guinea pigs by unfamiliar adult males. It was hypothesized that this HPA-buffering effect was driven by social interactions between the two partners and may involve an activation of the prelimbic cortex. Therefore, the current study examined these potential associations. To limit social interactions, the adult male was anesthetized in one condition compared to another condition where the adult male remained conscious. …

The Use Of Doublecortin To Quantify The Effects Of Pharmacological Treatment On Neurogenesis And Functional Recovery After Stroke, Amber Lee Hensley

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Ischemic strokes account for 87% of all strokes and can have debilitating effects on language, sensory, and motor skills. Currently, tPA is the only medication approved by the FDA for the treatment of ischemic stroke, but the window of time to administer the drug is very small. In this thesis, we investigate the use of a simvastatin and fluoxetine drug combination (FS) as a possible treatment for ischemic stroke victims. To analyze the effects of FS on neurogenesis and functional recovery, we utilize the Montoya Staircase and quantify the amount of neurogenesis using doublecortin. Although the results of this study …

Examination Of A Post-Stroke Drug Treatment For Its Effect On Blood Brain Barrier Permeability, And Gene Expression Changes In The Peri-Infarct Region, Ankita Anil Patel

Browse all Theses and Dissertations

In this current study, we have investigated this a combination of fluoxetine, simvastatin and ascorbic acid administered daily beginning at 20-26 hours after stroke induction. We hope to understand therapeutic abilities by studying its effectiveness on the blood brain barrier permeability and gene expression changes of the microglial subtypes involved in neuro-inflammation and neurogenesis factors in the peri-infarct region. Our results indicate that S-enantiomer of fluoxetine may be more beneficial compared to the R-enantiomer. The S-enantiomer was effective in tightening the blood brain barrier in contrast to the R-enantiomer, in which the latter showed a greater Evans Blue dye permeability …

Intrinsic And Extrinsic Regulation Of Pineal Melatonin Rhythms, Ye Li

Theses and Dissertations--Biology

Circadian rhythm is a biological rhythm with period of about 24 hours. Circadian rhythm is universal in phyla from bacteria to mammals and exists in different level from gene expression to behavior. Circadian system consists of three components: 1) a self-sustained oscillator; 2) an input pathway which can alter the phase of the oscillator; and 3) an output such as gene expression, enzyme activity, hormone production, heart rate, body temperature or locomotor activities. The way the oscillator regulates its outputs is complicated, in that on one hand usually the oscillator is not the only factor affecting the outputs, and on …

The Effects Of Exercise Intensity On Acylated Ghrelin, Active Glucagon-Like Peptide-1, And Appetite: Examining The Potential Involvement Of Interleukin-6, Hashim Islam

Theses and Dissertations (Comprehensive)

Interleukin-6 (IL-6) stimulates the release of appetite-regulating hormones in animals and associates with decreased energy intake in humans. Thus, IL-6 may contribute to the intensity- dependent effects of exercise on appetite-related parameters. The purpose of this study was to examine the effects of exercise intensity on IL-6, appetite-regulating hormones, and appetite perceptions. Eight active young males completed four sessions: 1) Moderate-intensity continuous training (MICT; 30 min running, 65% VO2max); 2) High-intensity continuous training (HICT; 30 min running, 85% VO2max); 3) Sprint interval training (SIT; 4 x 30 sec “all-out” running bouts separated by 4 min recovery); 4) Control (CTRL; no …

Anthropomorphic Denial Of Fish Pain, Lynne U. Sneddon, Matthew C. Leach

Animal Sentience

Key (2016) affirms that we do not know how the fish brain processes pain but denies — because fish lack a human-like cortex — that fish can feel pain. He affirms that birds, like fish, have a singly-laminated cortex and that the structure of the bird brain is quite different from that of the human brain, yet he does not deny that birds can feel pain. In this commentary we describe how Key cites studies that substantiate mammalian pain but discounts the same kind of data as evidence of fish pain. We suggest that Key's interpretations are illogical, do not …

The Effect Of Pre-Deployment Physiology As A Predictor Of Post-Traumatic Stress Disorder Among A Sample Of United States Army National Guard And Reserve Soldiers, David J. Rothman

Theses and Dissertations

Potential risk factors for development of Post-Traumatic Stress Disorder (PTSD) are still unclear. One potential risk factor for the development of PTSD is an individual’s cardiovascular reactivity and recovery in response to stressor tasks. The current study was conducted with 763 Army National Guard and Army Reserve soldiers. Participants completed a stressful induction along with self-report measures prior to deployment. Post-deployment, self-report measures were completed to assess PTSD symptomatology and experiences related to deployment and combat. Multiple regression was used to determine the ability of blood pressure response to stress to predict PTSD symptoms immediately and one-year after return from …

The Reproducibility And Validity Of Using The Dmax Method To Predict Physical Working Capacity At Fatigue Threshold, Joshua Riffe

Electronic Theses and Dissertations

Although the original (ORG) physical working capacity at fatigue threshold (PWCFT) assessment has demonstrated strong reliability and sensitivity to training and/or nutritional interventions, limitations may exist regarding the method of PWCFT estimation. However, a relatively new mathematical method, called Dmax, has been shown to be objective and reliable when estimating metabolic and neuromuscular fatigue thresholds. To date, however, no study has examined whether the Dmax method for estimating the work rate at PWCFT is similar to the ORG method. PURPOSE: The purpose of this study is to evaluate the reliability and validity of the Dmax-EMG method for estimating and tracking …

Resistance Exercise Elicits Selective Mobilization And Adhesion Characteristics Of Granulocytes And Monocyte Subsets, Adam Jajtner

Electronic Theses and Dissertations

Resistance exercise eliciting muscle damage results in an immune response, leading to increases in circulating cytokines, and immune cell mobilization. Classical monocytes respond to muscle damage, however, little is known about the intermediate or nonclassical monocyte response to resistance exercise. Moreover, the impact of polyphenol supplementation in conjunction with resistance exercise on the innate immune response is unknown. The purpose of this study was to examine the immune response following resistance exercise with (PPB) and without (PL) polyphenol supplementation. Thirty-nine untrained men were randomized into three groups: PPB (n=13, 21.8±2.5yrs, 171.2±5.5cm, 71.2±8.2kg), PL (n=15, 21.6±2.5yrs, 176.5±4.9cm, 84.0±15.7kg) or a control …

An Analysis Of Plasticity In The Rat Respiratory System Following Cervical Spinal Cord Injury And The Application Of Nanotechnology To Induce Or Enhance Recovery Of Diaphragm Function, Janelle Lorien Walker

Wayne State University Dissertations

Second cervical segment spinal cord hemisection (C2Hx) results in ipsilateral hemidiaphragm paralysis. However, the intact latent crossed phrenic pathway can restore function spontaneously over time or immediately following drug administration.

WGA bound fluorochromes were administered to identify nuclei associated with diaphragm function in both the acute and chronic C2Hx models. WGA is unique in that it undergoes receptor mediated endocytosis and is transsynaptically transported across select physiologically active synapses. Comparison of labeling in the acutely injured to the chronically injured rat provided an anatomical map of spinal and supraspinal injury induced synaptic plasticity. The plasticity occurs over time in the …

The Role Of Oxidative Stress In The Establishment Of Resistance To Cisplatin In Epithelial Ovarian Cancer Cells, Jimmy Belotte

Wayne State University Dissertations

Epithelial ovarian cancer is the deadliest of all gynecologic cancers with an estimated 22,280 new cases and 14,240 deaths expected in 2016 in the US alone. This high mortality rate can be partially attributed to a lack of universal screening and the development of resistance to the recommended chemotherapeutics. Typically, the treatment of ovarian cancer requires both cytoreductive surgery (CRS) and platinum/taxane combination chemotherapy. Initially, 50–80% of patients with advanced disease will achieve complete clinical response. Unfortunately, most will relapse within 18 months with chemoresistant disease. Thus, understanding the mechanisms of platinum resistance is critical in order to improve the …

Calponin And Cytoskeleton Dynamics In Macrophage Functions And The Pathogenesis Of Atherosclerosis, Rong Liu

Wayne State University Dissertations

Arterial atherosclerosis is an inflammatory disease. Macrophages play a major role in the pathogenesis and progression of atherosclerotic lesions. Modulation of macrophage function is a therapeutic target for the treatment of atherosclerosis. Calponin is an actin-filament-associated regulatory protein that inhibits the activity of myosin-ATPase and dynamics of the actin cytoskeleton. Encoded by the Cnn2 gene, calponin isoform 2 is expressed at significant levels in macrophages. Deletion of calponin 2 increases macrophage migration and phagocytosis. In the present study, we investigated the effect of deletion of calponin 2 in macrophages on the pathogenesis and development of atherosclerosis. The results showed that …

Mathematical Modeling Of Heart Rate Deflection Point In Relation To Respiratory Compensation And Treadmill Running Performance, Kayla Baker

Electronic Theses and Dissertations

Heart rate deflection point (HRDP), identified as the second breakpoint in the "intensity / heart rate" relationship, is indicative of the anaerobic threshold (AT). This point can be determined via bi-segmental linear regression (2SEG) or through use of the maximal distance model (D-max); however, the relationship between these methods has yet to be investigated 2. Purpose: To compare the use of 2SEG and D-max methods to determine HRDP and to examine the relationship between these values with a metabolic threshold, respiratory compensation point (RCP), as well as running performance [5,000 meter treadmill time trial (5Ktime)]. Methods: Nineteen recreationally active men …

A Plan For The Implementation And Evaluation Of Diet Education In Type 2 Diabetes, Soy Ramsumeer

Walden Dissertations and Doctoral Studies

Abstract

Type 2 Diabetes Mellitus (T2DM) is the seventh leading cause of death in the United States with a projected increase of 552 million people worldwide who will be affected with this illness by 2030. The need to address this issue is vital to prevent complications and reduce healthcare spending. The DNP project is aimed at planning and designing a nutritional education program tailored toward specific ethnic groups in order to increase knowledge in making healthy food choices. This project is intended to educate Registered Nurses (RNs) on nutrition so that they can offer dietary knowledge to T2DM patients. Additional …

Nanosecond Pulsed Platelet-Rich Plasma (Nsprp) Improves Mechanical And Electrial Cardiac Function Following Myocardial Reperfusion Injury, Barbara Y. Hargrave, Frency Varghese, Nektarios Barabutis, John Catravas, Christian Zemlin

School of Medical Diagnostics & Translational Sciences Publications

Ischemia and reperfusion (I/R) of the heart is associated with biochemical and ionic changes that result in cardiac contractile and electrical dysfunction. In rabbits, platelet-rich plasma activated using nanosecond pulsed electric fields (nsPRP) has been shown to improve left ventricular pumping. Here, we demonstrate that nsPRP causes a similar improvement in mouse left ventricular function. We also show that nsPRP injection recovers electrical activity even before reperfusion begins. To uncover the mechanism of nsPRP action, we studied whether the enhanced left ventricular function in nsPRP rabbit and mouse hearts was associated with increased expression of heat-shock proteins and altered mitochondrial …

Diabetes Mellitus Type 2: A Quality Improvement And Patient Safety Initiative, Remeliza Navarrete Tukay

Walden Dissertations and Doctoral Studies

The purpose of the quality improvement (QI) project was to examine the relationship between amended nursing education concerning diabetes mellitus (DM) Type 2 self-care management incorporating Tune in, Explore, Assist, Communicate, and Honor (TEACH) and Motivational Interviewing (MI) strategies and techniques and the Glycosylated hemoglobin (HgbA1C) of veteran patients with uncontrolled diabetes. The target sample included the 2 licensed practical nurses and 2 registered nurses assigned to 2 primary care teams, and the 10 purposively sampled patients with uncontrolled DM Type 2 from each team. The nurses' competencies were measured through descriptive comparison before and after nursing education implementation using …

Cardiac Calcium Atpase Dimerization Measured By Fluorescence Resonance Energy Transfer And Chemical Cross-Linking, Daniel Blackwell

Dissertations

The cardiac sarco/endoplasmic reticulum calcium ATPase (SERCA) establishes the intracellular calcium gradient across the sarcoplasmic reticulum membrane. It has been proposed that SERCA forms homo-oligomers that increase the catalytic rate of calcium transport. We investigated SERCA oligomerization in rabbit left ventricular myocytes using a photoactivatable cross-linker. Western blotting of cross-linked SERCA revealed higher molecular weight species consistent with SERCA oligomerization. Fluorescence resonance energy transfer (FRET) measurements in cells transiently transfected with fluorescently-labeled SERCA2a revealed that SERCA readily forms homo-dimers. These dimers formed in the absence or presence of the SERCA regulatory partner, phospholamban (PLB) and were unaltered by PLB phosphorylation …

Critical Role Of C-Jun N-Terminal Kinase Signaling In Binge Alcohol-Driven Atrial Arrhythmic Remodeling, Jiajie Yan Yan

Dissertations

Holiday Heart Syndrome (HHS) is cardiac arrhythmia induced by binge alcohol consumption, a drinking pattern affects 38 million adults in our society. Atrial fibrillation (AF) is the most frequently diagnosed arrhythmia in HHS and causes significant morbidity and mortality. Despite the intensive prevention effort nationwide, the binge drinking population keeps rising. However, no effective treatment strategies are available for binge drinking induced AF due to the unknown underlying mechanisms. In this thesis, I aim to elucidate the mechanisms of binge alcohol-promoted atrial arrhythmogenicity by using a mouse model of repeated binge alcohol exposure established in the Ai Lab.

The Ai …

High Affinity Block Of Icl,Swell By Thiol-Reactive Small Molecules, Sung H. Park

Theses and Dissertations

Ebselen (Ebs) is considered as a glutathione peroxidase (GPx) mimetic and primarily thought to function by scavenging intracellular reactive oxygen species (ROS). Previous to our work, Deng et al. (2010a) demonstrated complete block of I_Cl,swell with 15 microM Ebs following endothelin-1 (ET-1) induced activation of the current in cardiomyocytes. This block was presumed to take effect mainly via the quenching of ROS. Nonetheless, our work with DI TNC1 astrocytes strongly emphasizes that Ebs might function by an alternative mechanism based on its kinetic profile in blocking I_Cl,swell. Our experiments showed that 45 nM Ebs can fully block …

Changes In Brain Water Content In The Rainbow Trout (Oncorhynchus Mykiss) And In The Goldfish (Carassius Auratus) Due To High External Ammonia Exposure, Phillip Q.H. Pham-Ho

Theses and Dissertations (Comprehensive)

In fishes, hyperammonemia may occur following feeding or exposure to abnormally high concentrations of environmental ammonia due to sewage effluents, agricultural run-off and in crowded aquaculture pens. Increased internal ammonia can result in hyperactivity, convulsions, coma and death. In mammals, it is also associated with potentially fatal brain edema, in which the accumulation of intracellular water results in swelling, increased intracranical pressure and herniation leading to death. Recently it was shown that rainbow trout (Oncorhynchus mykiss) and goldfish (Carassius auratus) experience brain swelling following exposure to high external ammonia (HEA). However, the mechanism of ammonia-induced brain …