Physiology Commons^™

Age-Associated Metabolic And Morphologic Changes In Mitochondria Of Individual Mouse And Hamster Oocytes, Fatma Simsek-Duran, Fang Li, Wentia Ford, R. James Swanson, Howard W. Jones Jr., Frank J. Castora

Biological Sciences Faculty Publications

Background: In human oocytes, as in other mammalian ova, there is a significant variation in the pregnancy potential, with approximately 20% of oocyte-sperm meetings resulting in pregnancies. This frequency of successful fertilization decreases as the oocytes age. This low proportion of fruitful couplings appears to be influenced by changes in mitochondrial structure and function. In this study, we have examined mitochondrial biogenesis in both hamster (Mesocricetus auratus) and mouse (Mus musculus) ova as models for understanding the effects of aging on mitochondrial structure and energy production within the mammalian oocyte.

Methodology/Principal Findings: Individual metaphase …

The Relationship Between Heart Rate Variability, Shooting Experience, And Tactical Pistol Performance, Andrew Gerard Thompson

Human Movement Studies & Special Education Theses & Dissertations

PURPOSE: At rest and during a tactical pistol competition classifier, heart rate variability (HRV) time-domain, high frequency (HF), low frequency (LF), total power (TP), and inter-beat-interval (IBI) measurements were examined in 28, healthy subjects (37 ± 11 yr old, 180 ± 7 cm, 90.6 ± 16.9 kg) for correlations to performance. METHODS: Resting measurements were taken supine in a quiet, dimmed room, during metronomic breathing. The shooting task consisted of three stages, comprised of several strings each. The strings were initiated by a starting buzzer and involved several tactical maneuvers, including holster draws, engaging multiple targets, firing on the move, …

Treatment Of Aortic Heart Valve Conduit With Glutamine And Heat Shock As A Means To Deter The Constituent Cellular Population From Becoming Apoptotic, Alyce Marie Linthurst Jones

Theses and Dissertations in Biomedical Sciences

Cryopreserved allograft heart valves represent the best solution for a patient with a failing heart valve. However, the constituent cells become apoptotic and within months of transplant the heart valve becomes acellular and the recipient's cells do not repopulate the allograft (3, 51). A strategy to prevent this situation would be to minimize or prevent apoptosis from occurring by strategically altering steps during heart valve processing. Recently it has been demonstrated that: 1) Heat shock protein 70 is a negative modulator of the apoptotic cascade; 2) Cells in culture exposed to hypothermic conditions produce heat shock protein 70 upon rewarming; …

The Influence Of Interlimb Differences On Anterior Cruciate Ligament Injury Risk Factors In Female Collegiate Soccer Athletes, Eric Kevin Greska

Human Movement Studies & Special Education Theses & Dissertations

Female collegiate soccer athletes suffer anterior cruciate ligament (ACL) injuries at a rate of 0.31 per 1000 athlete-exposures, with 67% of injuries expected to be from a non-contact mechanism. As well, previous studies have demonstrated dissimilarity in non-contact ACL injuries relative to dominant and non-dominant legs; with the non-dominant leg being the involved side in 48-67% of reported incidents. The aim of this study was to examine lower extremity neuromechanics relative to leg dominance during an unanticipated sidestep cutting task, with differing states of fatigue and training.

Twenty female collegiate soccer players performed three valid trials for both the dominant …

The Effects Of Acute Hypoxia On Marksmanship, Chelsea Mary Moore

Human Movement Studies & Special Education Theses & Dissertations

Expeditionary warfighters in the military have the responsibility of being ready to deploy on short notice to any part of the world and be able to effectively complete their mission even if deployed to altitude. Studies demonstrate that altitude can significantly effect heart rate, ventilation rate, arterial oxygen saturation (SaO2) and cognition. Decrements in these areas could lead to a reduction in shooting accuracy. Only two studies have looked at the effects of marksmanship at various altitudes and their results are inconclusive. This study evaluated the effects of acute hypoxia on marksmanship and the effect of a physical exertion task …

Electric Field Exposure Triggers And Guides Formation Of Pseudopod-Like Blebs In U937 Monocytes, Mikhail A. Rassokhin, Andrei G. Pakhomov

Bioelectrics Publications

We describe a new phenomenon of anodotropic pseudopod-like blebbing in U937 cells stimulated by nanosecond pulsed electric field (nsPEF). In contrast to "regular," round-shaped blebs, which are often seen in response to cell damage, pseudopod-like blebs (PLBs) formed as longitudinal membrane protrusions toward anode. PLB length could exceed the cell diameter in 2 min of exposure to 60-ns, 10-kV/cm pulses delivered at 10-20 Hz. Both PLBs and round-shaped nsPEF-induced blebs could be efficiently inhibited by partial isosmotic replacement of bath NaCl for a larger solute (sucrose), thereby pointing to the colloid-osmotic water uptake as the principal driving force for bleb …

Aging, Neuromuscular Decline, And The Change In Physiological And Behavioral Complexity Of Upper-Limb Movement Dynamics, S. Morrison, K. M. Newell

Rehabilitation Sciences Faculty Publications

Aging is characterized by a general decline in physiological and behavioral function that has been widely interpreted within the context of the loss of complexity hypothesis. In this paper, we examine the relation between aging, neuromuscular function and physiological-behavioral complexity in the arm-hand effector system, specifically with reference to physiological tremor and isometric force production. Experimental findings reveal that the adaptive behavioral consequences of the aging-related functional decline in neurophysiological processes are less pronounced in simple motor tasks which provides support for the proposition that the motor output is influenced by both extrinsic (e.g., task related) and intrinsic (e.g., coordination, …

An Extracellular Domain Of The Accessory Beta1 Subunit Is Required For Modulating Bk Channel Voltage Sensor And Gate, Aleksandra Gruslova, Iurii Semenov, Bin Wang

Bioelectrics Publications

A family of tissue-specific auxiliary beta subunits modulates large conductance voltage- and calcium-activated potassium (BK) channel gating properties to suit their diverse functions. Paradoxically, beta subunits both promote BK channel activation through a stabilization of voltage sensor activation and reduce BK channel openings through an increased energetic barrier of the closed-to-open transition. The molecular determinants underlying beta subunit function, including the dual gating effects, remain unknown. In this study, we report the first identification of a beta1 functional domain consisting of Y74, S104, Y105, and I106 residues located in the extracellular loop of beta1. These amino acids reside within two …

In Vitro Measurements Of Tracheal Constriction Using Mice, Iurii Semenov, Jeremiah T. Herlihy, Robert Brenner

Bioelectrics Publications

Transgenic and knockout mice have been powerful tools for the investigation of the physiology and pathophysiology of airways(1,2). In vitro tensometry of isolated tracheal preparations has proven to be a useful assay of airway smooth muscle (ASM) contractile response in genetically modified mice. These in vitro tracheal preparations are relatively simple, provide a robust response, and retain both functional cholinergic nerve endings and muscle responses, even after long incubations. Tracheal tensometry also provides a functional assay to study a variety of second messenger signaling pathways that affect contraction of smooth muscle. Contraction in trachea is primarily mediated by parasympathetic, cholinergic …

First Transcriptome Of The Testis-Vas Deferens-Male Accessory Gland And Proteome Of The Spermatophore From Dermacentor Variabilis (Acari: Ixodidae), Daniel E. Sonenshine, Brooke W. Bissinger, Noble Egekwu, Kevin V. Donohue, Sayed M. Khalil, R. Michael Roe

Biological Sciences Faculty Publications

Ticks are important vectors of numerous human diseases and animal diseases. Feeding stimulates spermatogenesis, mating and insemination of male factors that trigger female reproduction. The physiology of male reproduction and its regulation of female development are essentially a black box. Several transcriptomes have catalogued expression of tick genes in the salivary glands, synganglion and midgut but no comprehensive investigation has addressed male reproduction and mating. Consequently, a new global approach using transcriptomics, proteomics, and quantitative gene expression is needed to understand male reproduction and stimulation of female reproduction.This first transcriptome to the reproductive biology of fed male ticks, Dermacentor variabilis …

Plantar Pressure Distribution Differences In Physically Active Individuals With And Without Functional Ankle Instability During A Side Cut, J'Nai Pittman

Human Movement Studies & Special Education Theses & Dissertations

Context: Functional ankle instability (FAI) is a popular condition in the physically active population. There is a surplus of evidence to demonstrate how FAI decreases proprioception, balance, and overall athletic performance. However, to date there is no evidence that demonstrates how F AI correlates to alterations in plantar pressure distribution differences in a physically active population while completing athletic tasks. Objective: To assess differences in plantar pressure distributions while completing a side cut in individuals with and without FAI. Design: Observational case-control. Setting: Outdoor field turf and research laboratory. Participants: Fifty ankles from physically active individuals (FAI: age= 21.47±3.02 yrs; …

The Comparison Of Hamstring/Quadriceps Strength Ratio In The Dominant Versus The Non-Dominant Leg Of Elite Female Artistic Gymnasts, Marina Moure

Human Movement Studies & Special Education Theses & Dissertations

The majority of the injuries in women's artistic gymnastics occur in the lower limbs (Marshall et al, 2007). Hamstring muscle strains are prevalent in sports that involve sprinting (Ekstrand & Quillist, 1985; Orchard, 2001; Croisier et al., 2008) with a high reinjury ratio (Orchard, 1993; Orchard, 1997). Recent studies have linked an increase of muscle injury rate in the lower limbs to strength muscle imbalance between legs and the relation between the hamstring and quadriceps strength (Croisier et al., 2002; Yamamoto, 2008; Sugiura et al, 2008). In order to determine if there is an imbalance in women's artistic gymnastics, forty-three …

Effect Of Body Mass And Added Mass On Treadmill Performance, Rachel Elizabeth Walker

Human Movement Studies & Special Education Theses & Dissertations

Strenuous physical activity in extreme environments is an important part of the activities of military personnel. The average infantry combat load for an infantry on the loaded tasks. Subjects performed an outdoor 4.8-km run and four maximal treadmill tests wearing the loads listed above. Subjects' pulmonary function was tested with load prior to each treadmill session. Peak values of heart rate, VO₂, exercise ventilation, and respiratory exchange ratio were measured at the end of each maximal treadmill test. Significant decreases were found in pulmonary function measures (FEV₁, FVC and MVV) with increasing load. Most peak responses …

Dose-Dependent Thresholds Of 10-Ns Electric Pulse Induced Plasma Membrane Disruption And Cytotoxicity In Multiple Cell Lines, Bennett L. Ibey, Caleb C. Roth, Andrei G. Pakhomov, Joshua A. Bernhard, Gerald J. Wilmink, Olga N. Pakhomova

Bioelectrics Publications

In this study, we determined the LD50 (50% lethal dose) for cell death, and the ED50 (50% of cell population staining positive) for propidium (Pr) iodide uptake, and phosphatidylserine (PS) externalization for several commonly studied cell lines (HeLa, Jurkat, U937, CHO-K1, and GH3) exposed to 10-ns electric pulses (EP). We found that the LD50 varied substantially across the cell lines studied, increasing from 51 J/g for Jurkat to 1861 J/g for HeLa. PS externalized at doses equal or lower than that required for death in all cell lines ranging from 51 J/g in Jurkat, to 199 J/g in CHO-K1. Pr …

Imbalanced Learning For Functional State Assessment, Feng Li, Frederick Mckenzie, Jiang Li, Guanfan Zhang, Roger Xu, Carl Richey, Tom Schnell, Thomas E. Pinelli (Ed.)

Electrical & Computer Engineering Faculty Publications

This paper presents results of several imbalanced learning techniques applied to operator functional state assessment where the data is highly imbalanced, i.e., some function states (majority classes) have much more training samples than other states (minority classes). Conventional machine learning techniques usually tend to classify all data samples into majority classis and perform poorly for minority classes. In this study, we implemented five imbalanced learning techniques, including random under-sampling, random over-sampling, synthetic minority over-sampling technique (SMOTE), borderline-SMOTE and adaptive synthetic sampling (ADASYN) to solve this problem. Experimental results on a benchmark driving test dataset show that accuracies for minority classes …

A Systematic Approach For Engagement Analysis Under Multitasking Environments, Guangfan Zhang, John Leddo, Roger Xu, Carl Richey, Tom Schnell, Frederick Mckenzie, Jiang Li, Thomas E. Pinelli (Ed.)

Electrical & Computer Engineering Faculty Publications

An overload condition can lead to high stress for an operator and further cause substantial drops in performance. On the other extreme, in automated systems, an operator may become underloaded; in which case, it is difficult for the operator to maintain sustained attention. When an unexpected event occurs, either internal or external to the automated system, a disengaged operation may neglect, misunderstand, or respond slowly/inappropriately to the situation. In this paper, we discuss a systematic approach monitor for extremes of cognitive workload and engagement in multitasking environments. Inferences of cognitive workload and engagement are based on subjective evaluations, objective performance …

The Correlation Between Visual Observations And Inclinometric Measurements For Special Tests Related To Patellofemoral Pain Syndrome, Erin Quada

Human Movement Studies & Special Education Theses & Dissertations

Patellofemoral pain syndrome (PFPS) is generally described as an insidious onset with pain increasing during running, jumping, ascending and descending stairs as well as sitting with the knees flexed for an extended period of time. Clinicians often use special tests in injury evaluations and range of motion assessments, usually making a visual observation of that status of the patient. The purpose of this study was to examine how interval measurements utilized in flexibility and range of motion assessments correlate with visual observations of special tests (active knee extension test, Thomas test, Ober's test, rectus femoris flexibility, and Craig's test). The …

The Effects Of Two Different Fatigue Protocols On Lower Extremity Kinematics And Kinetics During An Unanticipated Running Stop-Jump, David L. Quammen

Human Movement Studies & Special Education Theses & Dissertations

Altered neuromuscular control strategies and biomechanical movement risk factors are most likely to contribute to the increased incidence of non-contact ACL injury for female athletes. Neuromuscular control strategies and movement patterns are further altered when the effects of fatigue are present. The purpose of this study was to determine neuromechanical differences between two fatigue protocols [Slow Linear Oxidative Fatigue Protocol (SLO-FP) and Functional Agility Short-Term Fatigue Protocol (FASTFP)] when performing a running stop-jump task (RS). A sample of convenience of fifteen Division I female soccer players (age= 19.2 ± 0.8 years; height= 1.67 ± 0.05 m; mass= 61.7 ± 8.1 …

The Effects Of Two Types Of Fatigue On An Unanticipated Side-Step Cutting Task As Measured By Kinematic And Kinetic Variables, Shawn E. Lucci

Human Movement Studies & Special Education Theses & Dissertations

Fatigue has been shown to alter the neuromechanics of lower extremity. To date, no study has examined the effects of two types of fatigue on kinetics and kinematics. To determine neuromechanical differences between two fatigue protocols [Slow Linear Oxidative Fatigue Protocol (SLO-FP) and Functional Agility Short-Term Fatigue Protocol (FAST-FP)] when performing an unanticipated sidestep cutting task. Fifteen female collegiate soccer players (age=19.2±0.8 years; height=l.67±0.05m; mass=61.7±8.lkg) participated in this study. Participants were free of lower extremity injury at time of participation, and performed five successful trials of a sidestep cutting (SS) task pre and post fatigue protocols. For the SLO-FP, a …

The Effects Of Cocaine And Ecstasy On Cardiac Myocytes And The Intact Myocardium, David A. Tiangco

Theses and Dissertations in Biomedical Sciences

Cocaine and ecstasy are widely used illicit drugs. Both drugs have undergone intense scrutiny as information regarding their side-effects has become available. One important yet incomplete area of investigation pertains to their effects on the heart. The purpose of the current studies was to test the hypothesis that exposure to cocaine or ecstasy will adversely affect cellular homeostasis and normal heart function. Cultured cardiac myocytes (H9c2) and New Zealand White rabbits (Oryctolagus cuniculus) were used to measure the responses to various concentrations of cocaine or ecstasy at both the cellular and intact organ system levels. We observed that cocaine and …

Balance Training Reduces Falls Risk In Older Individuals With Type 2 Diabetes, Steven Morrison, Sheri R. Colberg, Mita Mariano, Henri K. Parson, Arthur I. Vinik

Rehabilitation Sciences Faculty Publications

OBJECTIVE - This study assessed the effects of balance/strength training on falls risk and posture in older individuals with type 2 diabetes.

RESEARCH DESIGN AND METHODS - Sixteen individuals with type 2 diabetes and 21 age-matched control subjects (aged 50-75 years) participated. Postural stability and falls risk was assessed before and after a 6-week exercise program.

RESULTS - Diabetic individuals had significantly higher falls risk score compared with control subjects. The diabetic group also exhibited evidence of mild-to-moderate neuropathy, slower reaction times, and increased postural sway. Following exercise, the diabetic group showed significant improvements in leg strength, faster reaction times, …

Spawning-Related Movements Of Barred Sand Bass Paralabrax Nebulifer, In Southern California: Interpretations From Two Decades Of Historical Tag And Recapture Data, E. T. Jarvis, Christi Linardich, C. F. Valle

Biological Sciences Faculty Publications

During the 1960s and 1990s, the California Department of Fish and Game tagged 8,634 barred sand bass in southern California, and 972 fish (11%) were recaptured. Tag returns suggest barred sand bass are transient aggregate spawners that form spawning aggregations consisting of both resident and migrant individuals. Spawning residency at a historic spawning location was estimated by the frequency of returns over time; most same-year returns (82%, n  =  141) were recaptured within a 7 to 35-day period. The maximum recapture distance was 92 km. The average (± SD) non-spawning season recapture distance from peak spawning season tagging locations was …

Isometric Isokinetic And Isotonic Strength Evaluation Of Subjects With Stable And Unstable Ankles, Lauren Nicole Snyder

Human Movement Studies & Special Education Theses & Dissertations

Injuries to the lateral ankle ligaments can result in ankle instability problems. Limited data exists in relation to isokinetic and isometric strength measures of the ankle, with an absence of data in relation to isotonic power measures. Therefore, the purpose of this study is to identify if there is a difference between subjects with unstable ankles and subjects with stable ankles with respect to isokinetic ankle strength, isometric ankle strength, and isotonic ankle power. Eighteen subjects with ankle instability (age, 23.9 ± 2.81 years; height 170.4 ± 8.9 cm; mass 73 ± 18.9 kg) and eighteen subjects with healthy ankles …

Mechanisms Of Regulation Of Proximal Tubule Sodium Transporters In Obesity-Induced Hypertension, Marta A. Ambrozewicz

Theses and Dissertations in Biomedical Sciences

Hypertension is one of the common complications of obesity. Using a rat model of diet induced obesity and hypertension we investigated some of the mechanisms that are involved in regulation of blood pressure in obesity. The first aim of this study was to determine the role of proximal tubule transporters on the renal sodium handling in obese hypertensive (OP) and lean normotensive (OR) rats. An acute increase in renal perfusion pressure resulted in a blunted natriuretic response in OP vs. OR rats and indicated that increased sodium reabsorption in the proximal tubule is casual, at least in part, for hypertension …

The Effect Of Intensity Of Aerobic Training On Insulin Sensitivity, Carmine Richard Grieco Iii

Human Movement Studies & Special Education Theses & Dissertations

Epidemiological studies have demonstrated that the prevalence of type 2 diabetes is increasing. It is estimated that I 0. 7% of adults over the age of 20 years and 23.1 % of adults over the age of 60 years have diabetes (NIH, 2007). Individuals with type 2 diabetes are 2-4 times more likely to die from heart disease than those without diabetes and twice as likely to die of all-cause mortality (CDC, 2005). Additionally Mokdad et al. (2000) report that the prevalence of diabetes increased 33% between 1990 and 1998. Furthermore it has been estimated that nearly 26% of the …

Pge2 Receptor Subsets Mediate Hcg Regulation Of The Plasminogen Activator/Plasmin System In Primate Periovulatory Follicles, Nune Markosyan

Theses and Dissertations in Biomedical Sciences

Elevated follicular prostaglandin E2 (PGE2) levels are necessary for follicle rupture during ovulation. As the degradation of the follicle wall requires proteolysis, it was hypothesized that regulation of proteolysis by gonadotropins is PGE2 mediated.

There are four PGE2 receptors: EP1, EP2, EP3 and EP4. To determine which PGE2 receptors are present in monkey periovulatory follicles, granulosa cells and whole ovaries were collected 0, 12, 24, and 36 hours after administration of an ovulatory dose of human chorionic gonadotropin (hCG), or 36 hours after hCG from monkeys that received the prostaglandin (PG) synthesis inhibitor celecoxib. All EP receptor mRNAs were present …

Validation Of A New Method Of Estimating Vo2max Based On Vo2Reserve With Treadmill Exercise, Julie Lynn Ward

Human Movement Studies & Special Education Theses & Dissertations

The purpose of the current study was to establish and then validate a treadmill protocol to predict VO_2max based on VO₂Reserve.

Methods

A treadmill protocol was developed to elicit approximately 60-70% of heart rate reserve (HRR) in the fifth and sixth minutes of the final stage. The protocol involved walking on a treadmill at 3.0 mph, with grade increasing by 1 % each minute until 50% HRR was reached. Then, an additional 5 minutes of walking at the final grade was performed. A study population of 60 volunteers (30 male; 30 female), considered low risk by ACSM …

The Effect Of Intensity Of Aerobic Vo2max Resting Heart Rate And Blood Pressure, Shannan Elizabeth Gormley

Human Movement Studies & Special Education Theses & Dissertations

The AGSM recommends 20 to 60 minutes of continuous or intermittent activity 3 to 5 times per week to maintain cardio respiratory fitness (Pollock et al., 1998). The 1996 Surgeon General's Report on Physical Activity and Health indicates that Americans are not meeting these physical activity recommendations (DHHS). Several clear consequences of physical inactivity are the appearance of cardiovascular disease risk factors, such as hypertension, hyperlipidemia and obesity. Two recent review articles suggested that higher intensity exercise will elicit a greater reduction in cardiovascular disease risk factors (Swain and Franklin, 2002; Swain and Franklin, 2006). In order to determine whether …

Assessment Of High School Athletic Coaches Knowledge Of The Prevention Recognition And Treatment Of Heat Illnesses, Erica Lea Borgia

Human Movement Studies & Special Education Theses & Dissertations

As evidenced by the current history of heat related deaths, heat-related illness is a vast problem in the United States. The purpose of this study was to assess the knowledge of high school athletic coaches' knowledge of the prevention, recognition, and treatment of exertional heat illnesses (EHI). A twenty-five question multiple choice assessment survey was developed to measure knowledge levels concerning the recognition, treatment, and prevention of EI-II. In addition, a questionnaire was utilized to collect demographic characteristics that could have had an effect on knowledge scores. The instrument was reviewed by a panel of experts for face and content …

The Effect Of Instruction Of Jump-Landing Motion Patterns And Impact Forces, Mary Elizabeth Joos

Human Movement Studies & Special Education Theses & Dissertations

The use of visual instruction could be a valuable tool in prevention strategies for anterior cruciate ligament injuries, especially in large group settings, through the alterations of jump-landing motion patterns and impact forces. The purpose of this study was to determine the effectiveness of two visual modeling cues in altering jump-landing motion patterns, as measured by the Landing Error Scoring System (LESS), and impact forces, vertical ground reaction force (PVGRF) and peak posterior ground reaction force (PPGRF). Seventy-three physically active individuals (age= 20.89 ± 1.72 years; height = 172 ± 9.87 cm; mass= 68.43 ± 15.97 kg) were baseline tested …