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Cholera Toxin Mediated Regulation Of The Expression Of Gq Alpha And G11 Alpha Gtp Binding Proteins, B H. Shah
Cholera Toxin Mediated Regulation Of The Expression Of Gq Alpha And G11 Alpha Gtp Binding Proteins, B H. Shah
Department of Biological & Biomedical Sciences
Previously it has been shown that persistent activation of the stimulatory adenylyl cyclase pathway with cholera toxin (CT) downregulates the Gs alpha polypeptide (80%) in a cAMP-independent manner in C6 glioma cells (Shah, 1997). This study was conducted to examine the short and long term effects of CT on the regulation of pertussis toxin-sensitive and -insensitive G proteins and their transcripts in C6 glioma cells. Treatment of C6 cells with CT (100 ng/ml) up to 16 h had no effect on either Gi or Gq/11 alpha proteins. However, prolonged exposure (24-48 h) caused increased expression of Gi (20-30%) and Gq/11 …