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Cholera Toxin Mediated Regulation Of The Expression Of Gq Alpha And G11 Alpha Gtp Binding Proteins, B H. Shah
Cholera Toxin Mediated Regulation Of The Expression Of Gq Alpha And G11 Alpha Gtp Binding Proteins, B H. Shah
Department of Biological & Biomedical Sciences
Previously it has been shown that persistent activation of the stimulatory adenylyl cyclase pathway with cholera toxin (CT) downregulates the Gs alpha polypeptide (80%) in a cAMP-independent manner in C6 glioma cells (Shah, 1997). This study was conducted to examine the short and long term effects of CT on the regulation of pertussis toxin-sensitive and -insensitive G proteins and their transcripts in C6 glioma cells. Treatment of C6 cells with CT (100 ng/ml) up to 16 h had no effect on either Gi or Gq/11 alpha proteins. However, prolonged exposure (24-48 h) caused increased expression of Gi (20-30%) and Gq/11 …
Co-Activation Of Gi And Gq Proteins Exerts Synergistic Effect On Human Platelet Aggregation Through Activation Of Phospholipase C And Ca2+ Signalling Pathways, B H. Shah, A Siddiqui, K A. Qureshi, M Khan, S Rafi, V A. Ujan, M Y. Yakoob, Y Yaqub, H Rasheed, S A. Saeed
Co-Activation Of Gi And Gq Proteins Exerts Synergistic Effect On Human Platelet Aggregation Through Activation Of Phospholipase C And Ca2+ Signalling Pathways, B H. Shah, A Siddiqui, K A. Qureshi, M Khan, S Rafi, V A. Ujan, M Y. Yakoob, Y Yaqub, H Rasheed, S A. Saeed
Department of Biological & Biomedical Sciences
Our previous studies have shown that subthreshold concentrations of two platelet agonists exert synergistic effects on platelet aggregation. Here we studied the mechanism of synergistic interaction of 5-hydroxytryptamine (5-HT) and epinephrine mediated platelet aggregation. We show that 5-HT had no or little effect on aggregation but it did potentiate the aggregation response of epinephrine. The synergistic interaction of 5-HT (1-5 microM) and epinephrine (0.5-2 microM) was inhibited by alpha2-adrenoceptor blocker (yohimbine; IC50= 0.4 microM), calcium channel blockers (verapamil and diltiazem with IC50 of 10 and 48 mM, respectively), PLC inhibitor (U73122; IC50=6 microM) and nitric oxide (NO) donor, SNAP (IC50=1.6 …
Atherosclerosis And Plaque Rupture: An Update, M N. Afzal, S A. Saeed, B H. Shah
Atherosclerosis And Plaque Rupture: An Update, M N. Afzal, S A. Saeed, B H. Shah
Department of Biological & Biomedical Sciences
No abstract provided.