Physiology Commons^™

Acclimation, Long-Term Repeatability, And Phenotypic Correlations Of Aerobic Metabolic Traits In The Gulf Killifish, Fundulus Grandis, Jessica E. Reemeyer

LSU New Orleans Theses and Dissertations

This research examined the effects of acclimation to lowered salinity, elevated temperature, and hypoxia on aerobic metabolism of the Gulf killifish, Fundulus grandis, a common estuarine resident of the Gulf of Mexico. Standard metabolic rate (SMR), maximum metabolic rate (MMR), absolute aerobic scope (AAS), and critical oxygen tension (P_crit) were each influenced by one or more acclimation treatments. Assessing the consistency of these traits measured in the same individuals over time, all were found to be significantly repeatable with no indication that the repeatability of any traits was affected by acclimation conditions. Significant correlations were found between …

Trade-Offs Between Survival And Reproduction In Starvation-Selected Drosophila Melanogaster, Tammara Beeghly

UNLV Theses, Dissertations, Professional Papers, and Capstones

Evolutionary outcomes are demonstrated by traits that affect an organism’s schedule of reproduction and survival. These life history traits are reflected in an organism’s physiology, development and behavior. Environmental changes, such as availability of nutritional resources, can profoundly affect evolutionary outcomes of individuals and populations. When shortages arise, there will be trade-offs in the allocation of resources, where one trait prevails at the expense of another.

In the laboratory, we can mimic conditions in nature and study the specific effects of the conditions that we re-create. In our case, over 100 generations of Drosophila melanogaster have been selected for starvation …

Eastern Collared Lizard (Crotaphytus Collaris) Population Declines In Ozark Landscapes: An Assessment Of Environmental Constraints., Casey L. Brewster

Graduate Theses and Dissertations

The Eastern Collared Lizard (Crotaphytus collaris) has experienced extensive population declines over the past half century in the Ozark Mountains. Previous research suggests that glade habitat degradation through woody vegetation encroachment is an important factor resulting in population declines. In this dissertation I used information on time-energy budgets to investigate the link between habitat degradation and shifts in life history traits likely resulting in population declines of Eastern Collared Lizards (Crotaphytus collaris) in the Ozarks. In chapter one, I addressed the influence of dense woody vegetation encroachment on age-specific growth, body size, body condition and reproduction of C. collaris in …

Stress And Body Composition Of Juvenile Alligator Snapping Turtles (Macrochelys Temminckii), Brandon Scott Tappmeyer

Graduate Theses/Dissertations

The alligator snapping turtle (Macrochelys temminckii), is a species of conservation concern that is the subject of multiple head-start and reintroduction efforts across its range. In captive propagation programs, producing offspring that are in optimal physiological condition maximizes the likelihood of success after release. The purpose of my study was to compare stress and body composition between one free-ranging reintroduced population and two captive populations. The two captive populations were both housed in southern Oklahoma, but one group was reared indoors whereas the other inhabited outdoor ponds at a national fish hatchery. I used circulating glucocorticoid (corticosterone) concentrations as an …

Comparative Thermal Ecology Of Coastal And Inland Pacific Rattlesnakes (Crotalus Oreganus), Hayley Layne Crowell

Master's Theses

Global biodiversity is declining as a direct result of anthropogenic climate change. Ectothermic species have become focal organisms for studying the ecological effects of altered climates due to the clear relationship between environmental temperatures and ectotherms’ basic physiological functions. Historically, examinations of these effects have focused heavily on heliothermic lizards, and most others have tended to focus on single populations or sympatric species within a single community. Addressing the longterm energetic implications of environmental temperature variation will provide valuable insight into the cascading physiological effects that certain populations or species may experience as a result of altered climates.

In this …

Effects Of Acute Hypoxia On Danio Rerio, Karly Crail

Williams Honors College, Honors Research Projects

The purpose of this research experiment was to determine the effects of acute hypoxia on the embryonic zebrafish (Danio rerio) cardiovascular system and assess the critical oxygen level at which embryonic function is compromised. This goal was achieved by exposing embryos to decreasing oxygen concentrations, (6.0 mg/L-0 mg/L) and observing the heart under an inverted microscope. Data analysis was conducted to determine end systolic volume, end diastolic volume, stroke volume, heart rate, and cardiac output. It was hypothesized that hypoxia would stimulate cardiac activity, because in early developmental stages, tissues are supplied with oxygen through bulk diffusion, and …

Body Condition Helps To Explain Metabolic Rate Variation In Wolf Spiders, Stella F. Uiterwaal, John P. Delong

School of Biological Sciences: Faculty Publications

1. Metabolism is the fundamental process that powers life. Understanding what drives metabolism is therefore critical to our understanding of the ecology and behavior of organisms in nature.

2. Metabolic rate generally scales with body size according to a power law. However, considerable unexplained variation in metabolic rate remains after accounting for body mass with scaling functions.

3. We measured resting metabolic rates (oxygen consumption) of 227 field-caught wolf spiders. Then, we tested for effects of body mass, species, and body condition on metabolic rate.

4. Metabolic rate scales with body mass to the 0.85 power in these wolf spiders, …

Quantifying The Morphological And Physiological Effects Of Drought, Grass Invasion, And Fire On Longleaf, Loblolly, And Slash Pine, Laura Young

College of Graduate Studies: Theses & Dissertations

Climate change is altering ecosystems on a global scale, creating novel ecological scenarios with which plant species must cope. Factors such as altered precipitation and fire regimes and non-native plant invasion may negatively affect native plant species, while interactions between these stressors could magnify their impacts. The complexity of multiple stressors and the effects they have on native pine seedlings are difficult to predict without evaluating their combinations in field experiments. In a multi-year study, I investigated the effects of drought and plant invasion on three southeastern pine species under pre and post-fire conditions. We planted longleaf (Pinus palustris …

Population Health Of Spotted Salamanders (Ambystoma Maculatum) In Created Vernal Pools: An Integrative Approach, Alice R. Millikin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Habitat creation is an important tool for conservation to counteract habitat loss and degradation. Vernal pools are susceptible to destruction due to limited detection, protection, and regulation. These wetlands provide fishless breeding habitat for many amphibian species including spotted salamanders (Ambystoma maculatum) in eastern North America. Determining whether created vernal pool habitat is successful is often determined by demographic data of colonizing populations. I suggest that hormone levels, population genetics, and disease prevalence can improve our understanding of population health in created habitat. The goal of this dissertation was to assess the health of spotted salamander larvae in …

Impacts Of Ammonia And Temperature On Freshwater Snail Behavior And Physiology., Megan Christine Dewhatley

Electronic Theses and Dissertations

Gastropods are one of the most imperiled groups of animals in North America, yet there are major gaps in the literature pertaining to pollutants and climate change, and especially sublethal impacts. This dissertation assesses the effects of climate warming and unionized ammonia (NH₃), one of the most abundant water pollutants, on the behavior and physiology of two caenogastropod snails: fine-ridged elimia (Elimia semicarinata) and Shawnee rocksnails (Lithasia obovata) (Gastropoda: Neotaenioglossa: Pleuroceridae). Righting behavior, or the movement used by snails to turn themselves right-side-up, was used as the main endpoint; delays in this behavior compromise …

Behavioral Insights Into Nociceptor Function: A Systematic Approach To Understanding Postsurgical And Neuropathic Pain Mechanisms In Rats, Max Odem

Dissertations and Theses (Open Access)

Postsurgical and neuropathic pain are each clinically common, and often associated with ongoing pain. Ongoing pain has been linked to ongoing activity (OA) in human C-fiber nociceptors. Preclinical studies using rodent neuropathic models have concentrated on allodynia driven by OA generated in non-nociceptive Aβ fibers, but little attention has been paid to postsurgical pain in sham controls or to C-fiber nociceptor OA promoting ongoing pain.

Operant assays that reveal negative motivational and cognitive aspects of voluntary pain-related behavior may be particularly sensitive to pain-related alterations. In the mechanical conflict (MC) test, rodents can freely choose to escape from a brightly …

The Thermal Ecology Of Sceloporus Occidentalis, Luis Patricio Burgos

Master's Theses

With temperatures rising globally, assessing the possible impacts of the changing climate becomes more and more urgent. Ectotherms are excellent indicators of potential climatic ramifications on biodiversity because of their heavy reliance on the environment for their thermoregulation. Studies have historically looked at thermal tolerance values to establish predictive models for population and species extinctions.

In chapter 1, we looked at recent studies that suggest that thermal tolerance may be a plastic trait and test the effects empirically. Most studies are based on captive lizards acclimated to laboratory conditions that do not necessarily reflect natural environments, and if thermal tolerance …

Reproductive Timing Of The Scarlet Shiner (Lythrurus Fasciolaris) In Northern Alabama, Bruce Stallsmith, Toacca Taylor, Chelsie Smith

Southeastern Fishes Council Proceedings

The Scarlet Shiner (Lythrurus fasciolaris) is a cyprinid species widely distributed in parts of the Ohio, Cumberland and Tennessee river drainages of the United States. The objective of this study was to determine the Scarlet Shiner’s reproductive schedule. Maturation of ovaries and oocytes was determined through the categorization of developmental stages from early maturing to ripe, along with the calculation of monthly gonadosomatic index (GSI) measurements over two reproductive seasons in 2012 and 2015. In both years reproductive competence began in April. Average monthly GSI for females peaked in May, followed by a slow decline through August. Average clutch …

Effect Of Torpor On Host Transcriptomic Responses To A Fungal Pathogen In Hibernating Bats, Kenneth A. Field, Brent J. Sewall, Jenni M. Prokkola, Gregory G. Turner, Marianne Gagnon, Thomas M. Lilley, J Paul White, Joseph S. Johnson, Christopher L. Hauer, Deeann M. Reeder

Faculty Journal Articles

Hibernation, the use of prolonged torpor to depress metabolism, is employed by mammals to conserve resources during extended periods of extreme temperatures and/or resource limitation. Mammalian hibernators arouse to euthermy periodically during torpor for reasons that are not well understood and these arousals may facilitate immune processes. To determine if arousals enable host responses to pathogens, we used dual RNA‐Seq and a paired sampling approach to examine gene expression in a hibernating bat, the little brown myotis (Myotis lucifugus). During torpor, transcript levels differed in only a few genes between uninfected wing tissue and adjacent tissue infected with …

Grapevine Vein Clearing Virus: Epidemiological Patterns And Construction Of A Clone, Cory Von Keith

Graduate Theses/Dissertations

Grapevine vein clearing virus (GVCV) is a recently discovered virus belonging to the Badnavirus genus. Characteristic to its name, the virus is associated with a disease where symptoms manifest as pronounced vein-clearing, resulting in severe berry deformation and vine decline in susceptible grape varieties. Sustainable production of wine is dependent on healthy plants. The associated disease is mainly found in Midwest vineyards. Attempts were made in this thesis to provide evidence of causality of the virus to the associated disease and to infer the historical path and migration pattern of GVCV. Conclusions and discussions will provide grape producers with the …

Local Adaptation Signatures In Thermal Performance Of The Temperate Coral Astrangia Poculata, Hannah Elise Aichelman

Biological Sciences Theses & Dissertations

The Northern Star Coral (Astrangia poculata) is an understudied temperate scleractinian coral that provides unique opportunities to understand the roles of phenotypic plasticity and local adaptation in coral physiological tolerance limits. Astrangia poculata inhabits hard bottom ecosystems from the northwestern Atlantic to the Gulf of Mexico and withstands an annual temperature range up to 20°C. Additionally, A. poculata is facultatively symbiotic and co-occurs in both symbiotic (“brown”) and aposymbiotic (“white”) states. Here, brown and white A. poculata were collected from Virginia (VA) and Rhode Island (RI), USA and exposed to heat (18-32°C) and cold (18-6°C) temperature assays during …

Radical Social Ecology As Deep Pragmatism: A Call To The Abolition Of Systemic Dissonance And The Minimization Of Entropic Chaos, Arielle Brender

Student Theses 2015-Present

This paper aims to shed light on the dissonance caused by the superimposition of Dominant Human Systems on Natural Systems. I highlight the synthetic nature of Dominant Human Systems as egoic and linguistic phenomenon manufactured by a mere portion of the human population, which renders them inherently oppressive unto peoples and landscapes whose wisdom were barred from the design process. In pursuing a radical pragmatic approach to mending the simultaneous oppression and destruction of the human being and the earth, I highlight the necessity of minimizing entropic chaos caused by excess energy expenditure, an essential feature of systems that aim …

Determining The Physiological And Behavioral Aspects Of Salinity Tolerance In The Asian Clam, Corbicula Fluminea, John Roden

Undergraduate Honors Theses

The Asian clam, Corbicula fluminea, is an invasive bivalve species that now occurs through most of the lower 48 United States. While a significant degree of salinity tolerance has been observed in C. fluminea, owing to its estuarine lineage, the physiological and behavioral responses to changes in salinity by these organisms are not completely understood. It was hypothesized that Corbicula would initially avoid elevated salinity levels (>1 g/L) behaviorally through valve closure, but would eventually have to open to dispel anaerobic waste products and deal with the salinity. To explore this, Corbicula were collected and put through …

Escaping The Arrhenius Tyranny: Metabolic Compensation During Exposure To High Temperature In Daphnia, Bret L. Coggins

Electronic Theses and Dissertations

Poikilothermic organisms experience trade-offs by differential physiological demands generated by temperature extremes. Many such organisms exhibit acclimatory effects, adjusting their metabolism and physiology to recently experienced temperatures. One such acclimatory effect is metabolic compensation, the deceleration of biological rates below Arrhenius expectations. Daphnia magna is eurythermal, and if acclimated to mildly stressful temperatures first, survives longer in lethal temperatures. This study examined the effect of ambient temperature (5°C-37°C) and acclimation history (lifetime at 10°C or 25°C) on the oxygen consumption rates of 8 genotypes of Daphnia with high or low acute temperature tolerance. There were decelerations of respiratory rates across …

Function Of The Posterior Laryngeal Constrictor In The Túngara Frog (Engystomops Pustulosus), Ashna Rahman Haque, Marcos Gridi-Papp

Pacific Research & Scholarship Day

No abstract provided.

The Impact Of Invasive Lionfish On The Feeding Performance Of Endemic Spotted Scorpionfish, Nathaniel Zbasnik

Masters Theses & Specialist Projects

Invasive species, such as the red lionfish, Pterois volitans, are damaging many ecosystems around the world by out-competing native species. However, little work has been done to determine if P. volitans have a direct influence on the feeding performance of native species with which they compete. This study examines the feeding performance in terms of suction pressure, kinematic timing, and excursion distances of spotted scorpionfish, Scorpaena plumieri. Through multiple trials it was examined how S. plumieri modulate their kinematic behavior in response to P. volitans and a conspecific. The creation of a smaller buccal cavity and a decrease in time …

The Mechanism Of Biotremor Production In The Veiled Chameleon (Chamaeleo Calyptratus), Samuel Tegge

Masters Theses & Specialist Projects

Vibratory communication has evolved in numerous animal groups, including insects, spiders, fishes, mammals, and was recently discovered in veiled chameleons (Chamaeleo calyptratus). I examined the mechanism by which C. calyptratus produce these biotremors. Muscle activity data were gathered during simulated anti-predator responses via electromyography (EMG) with simultaneous recordings of biotremor production using an accelerometer. I correlated EMG data with the accelerometer data to implicate the muscles responsible for the production of the biotremors. Mixed-effect linear regression models described the mechanism, and a model selection framework determined which model fit the data best. I then used an analysis of variance to …

Killi-Data News (Winter), Tyrone Genade

Killifish Research Review

Valued readers, it is with a heavy heart that I inform you that this is the last issue of Killi-Data News. The good news is that we will be back as Killifish Research Review. The dissolution of Killi-Data International created a prob- lem: how can the newsletter of a defunct organization live on without that organization? But other additional problems were building in the background. The first issue numbered 15 pages. The previous issue was 28 pages. The number of killifish related papers is increasing while time on our end (the editorial team) is running out. It takes a lot …

Killi-Data News (Fall), Tyrone Genade

Killifish Research Review

Many interesting papers have been published over the last three months. The large volume of papers coupled with the start of the new college semester (and the workload it brings) delayed this issue of Killi-Data News. But better late than never—or so I hope! In this issue Richard van der Laan provides an insightful review of the recent Aphanius papers as to their taxonomic implications and questions. The systematic issues he raises show the importance of the Molecular project: we need to get more samples of the various cyprinodontiforme families to resolve unsettled systematic and taxonomic issues. In the Next …

Killi-Data News (Summer), Tyrone Genade

Killifish Research Review

Over the last three months several interesting and exciting pa- pers have been published. By now most of you have heard the Nothobranchius fish poo news emanating from the Valenzano lab. That paper is reviewed and certainly has repercussions for the health of our captive fish. Polaçik et al have published interesting data with ramifications as to how we breed and incubate annual killifish. The big news in this issue is the paper from the Reznick lab which Jean Huber reviews. The contents of that paper goes to the heart of the question of just what a killifish is. The …

Killi-Data News (Spring), Tyrone Genade

Killifish Research Review

This is the start of Killi-Data News’ second year. In this first issue of the year we have the usual review of research pub- lications as well as input from Martin Reichard on his lab’s Nothobranchius research. Martin is responding to my reviews of his lab’s work in the previous edition. I am serious about making sure the content in this newsletter is reliable but I erred in the previous edition and Martin has written extensively to correct my mistake in the section “Erratum”. This reply is welcomed and owed to readers. I must confess that I don’t know everything …

Killi-Data News (Winter), Tyrone Genade

Killifish Research Review

This is the fourth edition, and concluding issue of the first volume, of Killi-Data News and I am happy that it has been well received by readers. At 25 pages this issue is a bit thin- ner than the last but this is because we agreed to make the cut-off for submissions the 1 st of December so we could get this edition out by the New Year. This is an exciting edition full of new species descrip- tions and analyses that will keep taxonomists busy for years to come. Costa has given us two molecular phylogenies on Melanorivulus as …

Killi-Data News (Spring), Tyrone Genade

Killifish Research Review

This is the start of Killi-Data News’ second year. In this first issue of the year we have the usual review of research pub- lications as well as input from Martin Reichard on his lab’s Nothobranchius research. Martin is responding to my reviews of his lab’s work in the previous edition. I am serious about making sure the content in this newsletter is reliable but I erred in the previous edition and Martin has written extensively to correct my mistake in the section “Erratum”. This reply is welcomed and owed to readers. I must confess that I don’t know everything …

Mrub_1325, Mrub_1326, Mrub_1327, And Mrub_1328 Are Orthologs Of B_3454, B_3455, B_3457, B_3458, Respectively Found In Escherichia Coli Coding For A Branched Chain Amino Acid Atp Binding Cassette (Abc) Transporter System, Bennett Tomlin, Adam Buric, Dr. Lori Scott

Meiothermus ruber Genome Analysis Project

In this project we investigated the biological function of the genes Mrub_1325, Mrub_1326, Mrub_1327, and Mrub_1328 (KEGG map number 02010). We predict these genes encode components of a Branched Chain Amino Acid ATP Binding Cassette (ABC) transporter: 1) Mrub_1325 (DNA coordinates 1357399-1358130 on the reverse strand) encodes the ATP binding domain; 2) Mrub_1326 (DNA coordinates 1358127-1359899 on the reverse strand) encodes the ATP-binding domain and permease domain; 3) Mrub_1327 (DNA coordinates 1359899-1360930 on the reverse strand) encodes a permease domain; and 4)Mrub_1328 (DNA coordinates 1711022-1712185 on the reverse strand) encodes the substrate binding domain. This system is not predicted to …

Environmental Effects On Drosophila Brain Development And Learning, Xia Wang, Amei Amei, J. Steven De Belle, Stephen P. Roberts

Biological Sciences Faculty Research & Creative Works

Brain development and behavior are sensitive to a variety of environmental influences including social interactions and physicochemical stressors. Sensory input in situ is a mosaic of both enrichment and stress, yet little is known about how multiple environmental factors interact to affect brain anatomical structures, circuits and cognitive function. In this study, we addressed these issues by testing the individual and combined effects of sub-adult thermal stress, larval density and early-adult living spatial enrichment on brain anatomy and olfactory associative learning in adult Drosophila melanogaster. In response to heat stress, the mushroom bodies (MBs) were the most volumetrically impaired among …