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Articles 1 - 30 of 60
Full-Text Articles in Environmental Health
The Role Of The State, Multinational Oil Companies, International Law & The International Community: Intersection Of Human Rights & Environmental Degradation Climate Change In The 21st Century Caused By Traditional Extractive Practices, The Amazon Rainforest, Indigenous People And Universal Jurisdiction To Resolve The Accountability Issue, Marcela Cabrera Luna
Master's Theses
Local, national and international conventions that protect indigenous sovereignty and their territories, where many of the resources are extracted from by multinational corporations (MNCs) particularly oil, the number one commodity of the world and cause of climate change, continue to be jeopardized because of the lack of a clear international legal framework that can protect them and potentially hold multinationals accountable for their actions. These practices are causing not only environmental issues to the indigenous and surrounding communities, but climate change is in fact, the real human rights issue of the 21st century and it affects everyone. By using …
The Smell Of Hopelessness, Juline Cariba Rachel Party
The Smell Of Hopelessness, Juline Cariba Rachel Party
Capstones
Rafts of sargassum seaweed kilometers wide have been invading the Caribbean for more than five years, but 2015 was the worst. Tons of seaweed stretch from Texas south to Trinidad and Tobago, with the Lesser Antilles suffering the most damage. Residents near beaches are exposed to an insidious and pernicious danger, scientists say, from fumes that smell like rotten eggs, emitted by the decaying algae. The odor also drives tourists away from beaches and restaurants, and the tourism economy is collapsing.Amid the algae crisis, an elderly woman, Virginie Mark, is trying to survive. She struggles with her health, finances and …
Impact Of Executive Order 13211 On Environmental Regulation: An Empirical Study, Elizabeth Ann Glass Geltman
Impact Of Executive Order 13211 On Environmental Regulation: An Empirical Study, Elizabeth Ann Glass Geltman
Publications and Research
A great deal has been written about the Energy Policy Act of 2005 exempting oil and gas operations using hydraulic fracturing from the purview of certain federal environmental laws. Far less attention has been paid to George W. Bush’s Executive Order 13211 (EO 13211), entitled “Actions Concerning Regulations that Significantly Affect Energy Supply, Distribution or Use.” The executive order requires federal agencies to evaluate the impact of federal regulations on “supply, distribution and use of energy.” This study examined the impact of EO 13211 on United States environmental and conservation regulations proposed and promulgated by federal agencies. The study found …
High Prevalence And Genetic Heterogeneity Of Rodent-Borne Bartonella Species On Heixiazi Island, China, Dong-Mei Li, Yong Hou, Xiu-Ping Song, Ying-Qun Fu, Gui-Chang Li, Ming Li, Marina E. Eremeeva, Hai-Xia Wu, Bo Pang, Yu-Juan Yue, Ying Huang, Liang Lu, Jun Wang, Qi-Yong Liu
High Prevalence And Genetic Heterogeneity Of Rodent-Borne Bartonella Species On Heixiazi Island, China, Dong-Mei Li, Yong Hou, Xiu-Ping Song, Ying-Qun Fu, Gui-Chang Li, Ming Li, Marina E. Eremeeva, Hai-Xia Wu, Bo Pang, Yu-Juan Yue, Ying Huang, Liang Lu, Jun Wang, Qi-Yong Liu
Environmental Health Sciences: Faculty Publications
We performed genetic analysis of Bartonella isolates from rodent populations from Heixiazi Island in northeast China. Animals were captured at four sites representing grassland and brushwood habitats in 2011 and examined for the prevalence and genetic diversity of Bartonella species, their relationship to their hosts, and geographic distribution. A high prevalence (57.7%) and a high diversity (14 unique genotypes which belonged to 8 clades) of Bartonella spp. were detected from 71 rodents comprising 5 species and 4 genera from 3 rodent families. Forty-one Bartonella isolates were recovered and identified, including B. taylorii, B. japonica, B. coopersplainsensis, B. grahamii, B. washoensis …
Macrobenthic Communities In The Northern Gulf Of Mexico Hypoxic Zone: Testing The Pearson-Rosenberg Model, Shivakumar Shivarudrappa
Macrobenthic Communities In The Northern Gulf Of Mexico Hypoxic Zone: Testing The Pearson-Rosenberg Model, Shivakumar Shivarudrappa
Dissertations
The Pearson and Rosenberg (P-R) conceptual model of macrobenthic succession was used to assess the impact of hypoxia (dissolved oxygen [DO] ≤ 2 mg/L) on the macrobenthic community on the continental shelf of northern Gulf of Mexico for the first time. The model uses a stress-response relationship between environmental parameters and the macrobenthic community to determine the ecological condition of the benthic habitat. The ecological significance of dissolved oxygen in a benthic habitat is well understood. In addition, the annual recurrence of bottom-water hypoxia on the Louisiana/Texas shelf during summer months is well documented.
The P-R model illustrates the decreasing …
Conversion Of Waste Cooking Oils Into Environmentally Friendly Biodiesel, Heidi Lai Ling Li, Peter Hoi Fu Yu
Conversion Of Waste Cooking Oils Into Environmentally Friendly Biodiesel, Heidi Lai Ling Li, Peter Hoi Fu Yu
Practical Social and Industrial Research Symposium
No abstract provided.
Β-Thujaplicin: A Soil Antifungal, Dennis J. Baumgardner
Β-Thujaplicin: A Soil Antifungal, Dennis J. Baumgardner
Journal of Patient-Centered Research and Reviews
Background: β-thujaplicin (β-Th), also known as hinokitiol, naturally occurs in cedar mulch, is found in personal care products and has in vitro antitumor activities. It is antibacterial and antifungal, but has not been tested on soil. Scedosporium apiospermum (Sce) is an emerging “extremophile” fungal pathogen found in built outdoor environments.
Purpose: Pilot β-Th as “natural” soil antimicrobial or for isolation of extremophiles, and to explore β-Th resistance as selective advantage to Sce in mulched landscape.
Methods: A variety of outdoor and indoor environments were used for 2 sets of 24 paired soil samples. Soil/H20 slurry (0.1 ml) was spread on …
A Tail Of Two Phages: Genomic And Functional Analysis Of Listeria Monocytogenes Phages Vb_Lmos_188 And Vb_Lmos_293 Reveal The Receptor-Binding Proteins Involved In Host Specificity, Aidan Casey, Kieran Jordan, Horst Neve, Aidan Coffey, Olivia Mcauliffe
A Tail Of Two Phages: Genomic And Functional Analysis Of Listeria Monocytogenes Phages Vb_Lmos_188 And Vb_Lmos_293 Reveal The Receptor-Binding Proteins Involved In Host Specificity, Aidan Casey, Kieran Jordan, Horst Neve, Aidan Coffey, Olivia Mcauliffe
Department of Biological Sciences Publications
The physical characteristics of bacteriophages establish them as viable candidates for downstream development of pathogen detection assays and biocontrol measures. To utilize phages for such purposes, a detailed knowledge of their host interaction mechanisms is a prerequisite. There is currently a wealth of knowledge available concerning Gram-negative phage-host interaction, but little by comparison for Gram-positive phages and Listeria phages in particular. In this research, the lytic spectrum of two recently isolated Listeria monocytogenes phages (vB_LmoS_188 and vB_LmoS_293) was determined, and the genomic basis for their observed serotype 4b/4e host-specificity was investigated using comparative genomics. The late tail genes of these …
Recursos Y Servicios Disponibles Para Personas Afectadas Por El Uso De Agroquímicos Tóxicos En Avia Terai, Chaco, Argentina / Resources And Services That Are Available To People Who Are Affected By The Use Of Toxic Agrochemicals In Avia Terai, Chaco, Argentina, David Andersen
Independent Study Project (ISP) Collection
Introducción: El Chaco es una provincia del norte de Argentina con los peores indicadores de salud y de menor ingreso. El estudio se desarrolló en Avia Terai, un pueblo que tiene cinco mil habitantes, está rodeado por cultivos que son fumigados con agroquímicos tóxicos y que tiene niveles muy altos de cáncer y de discapacidad. Muchas personas en Avia Terai trabajan con agroquímicos y viven cerca de los campos fumigados. La mayoría de las personas en el pueblo no tienen seguros de salud y usan el sector público. En el pueblo hay un Centro Integrador Comunitario (CIC), un hospital, dos …
Exposure Monitoring In Developing Countries, Luis Collado
Exposure Monitoring In Developing Countries, Luis Collado
Graduate Theses & Non-Theses
Formaldehyde is widely used in the healthcare industry. Formaldehyde is used as a disinfectant and sterilant. In addition, formaldehyde/formalin is used as fixative/ preservative of anatomical specimens in pathology departments. Individuals may experience adverse effects following short term exposure above 0.1ppm. Following a laboratory study in the early 1980s, the U.S. Environmental Protection Agency (EPA) classified formaldehyde as a human carcinogen in 1987.
Although the healthcare industry in the United States made major advancement in the safe use of formaldehyde/formalin since the implementation of the Occupational Health and Safety Administration (OSHA) Formaldehyde standard, other developing countries are still struggling with …
Bleaching, Disease, And Colonization: The Ecology Of Coral Health In Southeastern Nosy Be, Madagascar, Luella Allen-Waller
Bleaching, Disease, And Colonization: The Ecology Of Coral Health In Southeastern Nosy Be, Madagascar, Luella Allen-Waller
Independent Study Project (ISP) Collection
Coral reefs constitute irreplaceable networks of marine biodiversity as well as an important economic resource to many coastal communities in the tropics. Many factors threaten these fragile ecosystems worldwide: overfishing, pollution, ocean acidification, and increasing sea temperatures all interact to diminish reef-building coral health in a variety of ways. This study aims to characterize the taxonomic and spatial patterns of several acute negative health conditions affecting hard corals in and near Lokobe National Park, Nosy Be, Madagascar. Bleaching, coral disease, filamentous algal overgrowth, and soft coral colonization were surveyed at six fringing reef sites representing different ecological zones. While Acropora …
A Comparative Study Of Faecal Sludge Management In Malawi And Zambia: Status, Challenges And Opportunities In Pit Latrine Emptying, Rochelle Holm, James Madalitso Tembo, Bernard Thole
A Comparative Study Of Faecal Sludge Management In Malawi And Zambia: Status, Challenges And Opportunities In Pit Latrine Emptying, Rochelle Holm, James Madalitso Tembo, Bernard Thole
Faculty and Staff Scholarship
This review paper covers the issues of pit latrine emptying national policies and regulations with a focus on Malawi and Zambia. With 2.4 billion people worldwide still lacking improved sanitation facilities, developing countries need to look at policy, regulation and practice for household sanitation service provision with a new lens. What happens “next,” when improved sanitation facilities eventually become full? An emphasis on faecal sludge management has multiplied this important issue in the past few years. The authors compare the pit latrine emptying situation in Malawi and Zambia with a focus on status, challenges and opportunities. To build this comparison, …
Using Choice Experiment Valuation Methods To Measure Public Preference For A New National Park In Maine, Alexander G. Wilsterman
Using Choice Experiment Valuation Methods To Measure Public Preference For A New National Park In Maine, Alexander G. Wilsterman
Journal of Environmental and Resource Economics at Colby
With global population increasing faster than ever, the need to protect land from development is at an all-time high. This paper seeks the measure the public preference for a new national park located in northern Maine. A national park will both protect the land and inject a much-needed economic stimulus to the surrounding communities. The study uses the choice experiment valuation method to quantify its results. Through this revealed preference we can quantify which characteristics are most important to the public so that these characteristics may be considered if the project is ever approved.
Golf Courses In Maine: Land Type Valuation Versus A Hedonic Pricing Analysis, Gregory Ladd, Jason Buco
Golf Courses In Maine: Land Type Valuation Versus A Hedonic Pricing Analysis, Gregory Ladd, Jason Buco
Journal of Environmental and Resource Economics at Colby
Many studies have been conducted analyzing the economic impact of golf courses on the local communities. These studies examine economic impacts of tournaments, endorsements, and vacation expenses of the major golf courses in the United States. However, there is little research conducted on the environmental impact of Golf courses, specifically in Maine. This paper performs a hedonic pricing analysis using housing prices in proximity to golf courses and compares it to the valuation of various land types in Maine. Housing prices were collected in the towns of Auburn, and North Yarmouth both near and distant from the local golf course. …
Cost Benefit Analysis Of Café Standards Compared To The Alternative Fuel/Carbon Tax, Brian Levinson
Cost Benefit Analysis Of Café Standards Compared To The Alternative Fuel/Carbon Tax, Brian Levinson
Journal of Environmental and Resource Economics at Colby
The Cafe Standards were introduced in 1975 following the Oil Embargo. The goals of this program were simple. To reduce co2 emissions in vehicles by increasing a mandatory average mpg level for car manufacturers in the United States. Recently, in 2012, the Obama Administration set new Cafe Standard requirements for car manufacturers. By 2025, all new cars on the road in the United States must average 54.5 miles per gallon, which would double the current 27 mpg average in place right now. While the intentions of this policy are to reduce co2 emissions while at the same time increasing savings …
The Economics Of The Audubon Society's Sanctuary Program For Golf Courses, Dan Hyszczak
The Economics Of The Audubon Society's Sanctuary Program For Golf Courses, Dan Hyszczak
Journal of Environmental and Resource Economics at Colby
Millions of people play golf every year, and in 2011 Golf Courses gained $22 billion dollars in revenue. This statistic combined with golf’s inherent place in the natural environment lead to questions of value and development for golf course owners. In 1991, The Audubon Society created their Cooperative Sanctuary Program for Golf Courses (ACSP) to promote environmentally sustainable practices for golf courses and to recognize the courses that are the most environmentally in both the United States and around the world.
This paper builds off of prior research that connects golf course beauty with revenue by examining the effects of …
Preferences For Coral Reef And Fishery Management In Okinawa, Japan, Nils Carlson
Preferences For Coral Reef And Fishery Management In Okinawa, Japan, Nils Carlson
Journal of Environmental and Resource Economics at Colby
This study is an example of how a nonmarket valuation method – a choice experiment – can be used to influence and guide ecosystem conservation efforts. Using a choice experiment survey, this study estimates the willingness to pay (WTP) for certain hypothetical attributes of a restored and conserved coral reef in Okinawa, Japan. I find that an increase in the amount of fish available to catch in ten years as well as an increase in the extent and health of the coral reefs and the number of marine biodiversity found in the Okinawan waters after ten-years, both positively affect respondent’s …
An Exploratory Statistical Analysis Of The External And Internal Effects Of Art Museums In The United States, John Eder
Journal of Environmental and Resource Economics at Colby
An Exploratory Statistical Analysis of the External and Internal Effects of Art Museums in the United States
The Effects Of Land Conservation On Productivity, Robert Mccormick, Carolyn Fuwa
The Effects Of Land Conservation On Productivity, Robert Mccormick, Carolyn Fuwa
Journal of Environmental and Resource Economics at Colby
In this research the relationship between productivity of land conservation is analyzed using panel data of the GDP and acres conserved of all 50 U.S. states from 1998-2005. Two main theories of the conservation productivity relationship exist. The first maintains that conservation impedes productivity because it does not allow land cultivation. Conversely, the second theory maintains that land conservation has a positive effect on GDP due to the recreation and tourism it creates. Through the use of fixed effects and random effects regressions, the amount of land conserved per state is not a significant predictor of state GDP The GDP …
A Survey On Climate Change: How Beliefs Shape Responsibility, Connor P. Clancy, Sarah Beth Solomon
A Survey On Climate Change: How Beliefs Shape Responsibility, Connor P. Clancy, Sarah Beth Solomon
Journal of Environmental and Resource Economics at Colby
This paper examines the lack of awareness and feelings of personal responsibility for climate change. Previous literature suggests that while part of the United States population may be aware of climate change, they do not feel personally responsible for causing it despite feeling climate change’s effects just like everyone else. Furthermore, studies suggest that individuals also do not believe climate change will affect them now, but rather it is an issue for future generations. Using a survey, we examine which demographics and personal factors are most important in eliciting awareness for climate change, and furthermore, personal responsibility for its effects. …
Invited Introduction To Jerec, Noelwah Netusil
Invited Introduction To Jerec, Noelwah Netusil
Journal of Environmental and Resource Economics at Colby
No abstract provided.
Welcome By The Editor, Sahan T. M. Dissanayake
Welcome By The Editor, Sahan T. M. Dissanayake
Journal of Environmental and Resource Economics at Colby
No abstract provided.
Nanotechnology In The Real World: Redeveloping The Nanomaterial Consumer Products Inventory, Marina E. Vance, Todd Kuiken, Eric P. Vejerano, Sean P. Mcginnis, Michael F. Hochella Jr., David Rejeski, Matthew S. Hull
Nanotechnology In The Real World: Redeveloping The Nanomaterial Consumer Products Inventory, Marina E. Vance, Todd Kuiken, Eric P. Vejerano, Sean P. Mcginnis, Michael F. Hochella Jr., David Rejeski, Matthew S. Hull
Faculty Publications
To document the marketing and distribution of nano-enabled products into the commercial marketplace, the Woodrow Wilson International Center for Scholars and the Project on Emerging Nanotechnologies created the Nanotechnology Consumer Products Inventory (CPI) in 2005. The objective of this present work is to redevelop the CPI by leading a research effort to increase the usefulness and reliability of this inventory. We created eight new descriptors for consumer products, including information pertaining to the nanomaterials contained in each product. The project was motivated by the recognition that a diverse group of stakeholders from academia, industry, and state/federal government had become highly …
An Investigation Of The Effect Of Malathion On Adaptive Plasticity Of Pseudacris Sierra, Michael Jonathan Maples
An Investigation Of The Effect Of Malathion On Adaptive Plasticity Of Pseudacris Sierra, Michael Jonathan Maples
Master's Theses
This thesis is composed of two chapters. Chapter one reviews what is known about adaptive plasticity in response to predators, describes the physiological systems involved in such plasticity, and outlines the evolutionary consequences of adaptive plasticity. Chapter two describes a scientific experiment that investigates how malathion may impact adaptive plasticity in the Sierran Treefrog, Pseudacris sierra. Anuran tadpoles suffer high mortality rates due to predation. In response to strong selective forces relating to these high predation rates, tadpoles evolved the ability to adaptively respond to predators through morphological and behavioral plasticity. The morphological and behavioral responses are varied and depend …
Sustaining The Saco Estuary: Final Report 2015, Christine B. Feurt Ph D, Pamela A. Morgan, Mark D. O. Adams, Anna L. Bass, Carrie J. Byron, Michael C. Daley, Michael O. Esty, Noah G. Perlut, Kayla Smith, Tyler Spillane, Michelle M. Steen-Adams, James Sulikowski, Stephan I. Zeeman, Jacob Aman, Michele Dionne, Jeremy Miller, Kristin Wilson
Sustaining The Saco Estuary: Final Report 2015, Christine B. Feurt Ph D, Pamela A. Morgan, Mark D. O. Adams, Anna L. Bass, Carrie J. Byron, Michael C. Daley, Michael O. Esty, Noah G. Perlut, Kayla Smith, Tyler Spillane, Michelle M. Steen-Adams, James Sulikowski, Stephan I. Zeeman, Jacob Aman, Michele Dionne, Jeremy Miller, Kristin Wilson
Environmental Studies Faculty Publications
This study focuses on the Saco estuary, the tidal portion of the Saco River, which drains the largest watershed in southern Maine. With headwaters in the White Mountains of New Hampshire, the watershed encompasses more than 4,400 km2, and provides clean healthy drinking water to over 100,000 people living and working in communities in southern Maine.
When the study began in 2009, very little was known about the ecology of the Saco estuary. Researchers at the University of New England and the Wells National Estuarine Research Reserve employed the process of collaborative learning to bring together people who …
Cold Plasma Treatment Of Biodegradable Films And Smart Packaging, Shashi Pankaj
Cold Plasma Treatment Of Biodegradable Films And Smart Packaging, Shashi Pankaj
Doctoral
Cold plasma is an emerging technology offering many potential applications for food packaging. While it was originally developed to increase the surface energy of polymers, enhancing their adhesion and printability, it has recently emerged as a powerful tool for surface sterilisation of both food and food packaging materials. The food packaging industry is still dominated by petroleum derived polymers but in the past few decades there has been significant interest in the development of environment friendly, biodegradable green polymers. In this study, the interaction and effects of cold plasma with biopolymer based packaging materials (polylactic acid, zein, sodium caseinate, starch, …
Removal Of Heavy Metal Ions From Aqueous Solution Using Rice Husks-Based Adsorbents, Fanuel J Ligate, James E.G Mdoe
Removal Of Heavy Metal Ions From Aqueous Solution Using Rice Husks-Based Adsorbents, Fanuel J Ligate, James E.G Mdoe
Tanzania Journal of Science
This study reports on the preparation and characterization of rice husks-based adsorbents and their application in the removal of heavy metal ions from aqueous solution. Three different adsorbents were developed: rice husks ash (RHA), rice husks ash-derived silica (RHS) and triaminopropyl-silica hybrid (TSH-R). The prepared materials were characterized by acid titration, Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy, Scanning Electron Microscopy-Energy Dispersive X-ray (SEM-EDX) spectroscopy and nitrogen physisorption analysis. Results from the ATR-FTIR analysis indicated that pure silica was obtained from RHA and that after surface modification the triaminopropyl group was successfully attached onto the silica framework with a …
Assessment Of Heavy Metal Concentration In Water Around The Proposed Mkuju River Uranium Project In Tanzania, Firmi P. Banzi, Peter K. Msaki, Najat K. Mohammed
Assessment Of Heavy Metal Concentration In Water Around The Proposed Mkuju River Uranium Project In Tanzania, Firmi P. Banzi, Peter K. Msaki, Najat K. Mohammed
Tanzania Journal of Science
Effective verification for compliance with water quality standards in uranium mining in Tanzania requires data sensitive to monitor heavy metal concentration in water around the Mkuju River Uranium Project before mining commences. The area susceptible for pollution by the project was estimated using AERMOD dispersion model and found to cover about 1300 km2. Thirty one surface and groundwater samples were collected and analysed for heavy metals and physicochemical properties using ICP-MS and standards techniques, respectively. The physicochemical properties for water samples analysed ranges from 5.7 to 7.8 for pH, 2.8 to 80.2 mg/L for TDS and 15 to 534.5 mS/cm …
Anion-Responsive Metallopolymer Hydrogels For Healthcare Applications, Jiuyang Zhang, Jing Yan, Parasmani Pageni, Yi Yan, Adam Wirth, Yun-Ping Chen, Yali Qiao, Qian Wang, Alan W. Decho, Chuanbing Tang
Anion-Responsive Metallopolymer Hydrogels For Healthcare Applications, Jiuyang Zhang, Jing Yan, Parasmani Pageni, Yi Yan, Adam Wirth, Yun-Ping Chen, Yali Qiao, Qian Wang, Alan W. Decho, Chuanbing Tang
Faculty Publications
Metallopolymers combine a processable, versatile organic polymeric skeleton with functional metals, providing multiple functions and methodologies in materials science. Taking advantage of cationic cobaltocenium as the key building block, organogels could be simply switched to hydrogels via a highly efficient ion exchange. With the unique ionic complexion ability, cobaltocenium moieties provide a robust soft substrate for recycling antibiotics from water. The essential polyelectrolyte nature offers the metallopolymer hydrogels to kill multidrug resistant bacteria. The multifunctional characteristics of these hydrogels highlight the potential for metallopolymers in the field of healthcare and environmental treatment.
Quantitative Sequencing For The Determination Of Kdr-Type Resistance Allele (V419l,L925i, I936f) Frequencies In Common Bed Bug, Cimex Lectularius L., (Hemiptera: Cimicidae) Populations Collected From Israel, Daniel J. Palencar, Kyle J. Gellatly, Kyong-Sup Yoon, Kosta Y. Mumcuoglu, Uri Shalom, J Marshall Clark
Quantitative Sequencing For The Determination Of Kdr-Type Resistance Allele (V419l,L925i, I936f) Frequencies In Common Bed Bug, Cimex Lectularius L., (Hemiptera: Cimicidae) Populations Collected From Israel, Daniel J. Palencar, Kyle J. Gellatly, Kyong-Sup Yoon, Kosta Y. Mumcuoglu, Uri Shalom, J Marshall Clark
SIUE Faculty Research, Scholarship, and Creative Activity
Human bed bug infestations have dramatically increased worldwide since the mid-1990s. A similar phenomenon was also observed in Israel since 2005, when infestations were reported from all over the country. Two single nucleotide polymorphisms (V419L and L925I) in the bed bug voltage sensitive sodium channel confer kdr-type resistance to pyrethroids. Using quantitative sequencing (QS), the resistance allele frequencies of Israeli bed bug populations from across the country were determined. Genomic DNA was extracted from samples of 12 populations of bed bugs collected from Israel and DNA fragments containing the V419L or L925I and I936F mutations sites were PCR amplified. The …