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Articles 1 - 30 of 156
Full-Text Articles in Environmental Health
Integrating Space Use And Demography To Support Management Of An Endangered Alpine Ungulate, Danielle J. Berger
Integrating Space Use And Demography To Support Management Of An Endangered Alpine Ungulate, Danielle J. Berger
All Graduate Theses and Dissertations, Fall 2023 to Present
The places animals choose to live affect the food they find, the dangers they face, and the weather conditions they experience. These experiences influence whether individual animals survive and reproduce, which in turn affects the size and health of animal populations. Although scientists have long believed that animal movements play an important role in population trends, there has been limited real-world evidence showing this connection. Advances in GPS tracking technology now allow researchers to follow animals over long periods and better understand how movement influences survival. Using 20 years of GPS tracking data from the endangered Sierra Nevada bighorn sheep …
Terrestrial Abiotic Synthesis Of Simple Precursor Molecules Starting From Two Approaches: The Universality Of Carbon Reduction, Eloi Camprubi, Ben K.D. Pearce, Martina Preiner
Terrestrial Abiotic Synthesis Of Simple Precursor Molecules Starting From Two Approaches: The Universality Of Carbon Reduction, Eloi Camprubi, Ben K.D. Pearce, Martina Preiner
School of Integrative Biological & Chemical Sciences Faculty Publications
To address the chemistry responsible for life’s emergence, we examine the simple chemicals accessible on early Earth and the processes that could have transformed them. We focus on carbon reduction as a process central to the direct precursors of life. Two main approaches for the emergence of life’s building blocks and their associated functions are discussed. One approach investigates chemical routes for forming life’s building blocks via processes that worked differently from those of extant life, referred to as “unrestricted” prebiotic chemistries. The other approach establishes a direct mechanistic connection between prebiotic building blocks and extant biochemistry, referred to as …
Somatic Prosthetics For Planetary Resonance, Disha Dharesh Kumar R
Somatic Prosthetics For Planetary Resonance, Disha Dharesh Kumar R
Masters Theses
This thesis investigates how industrial design can transcend extractive technological paradigms in favor of relational interfaces that foster planetary attunement. Framing the climate crisis as a 'crisis of imagination', the research challenges the Western bifurcation of nature and culture by drawing on Indic cosmologies- which recognize stones, plants, and ecosystems as conscious at different levels and participants in a shared cosmic field. By synthesizing research in Biosemiotics, Quantum Information Pansycishm, and Neuroscience, the project redefines intelligence as a distributed, more-than-human phenomenon.
The research materializes as a speculative design artifact: a device that functions as a somatic prosthetic for planetary resonance. …
Acute Effects Of The Psychoactive Pollutant Fluoxetine On The Antipredator Behavior Of The Gulf Killifish (Fundulus Grandis), Semyra Reus
Honors Theses
Pharmaceutical contaminants are increasingly prevalent in aquatic environments, yet their sublethal effects on ecologically relevant behaviors remain poorly understood, particularly in estuarine systems. Selective serotonin reuptake inhibitors (SSRIs), such as fluoxetine, are of particular concern due to the conserved role of serotonin in regulating behavior across vertebrates. Here, we investigated how acute exposure to environmentally relevant concentrations of fluoxetine influences anti-predator behavior in Gulf killifish (Fundulus grandis), a key estuarine fish commonly found in saltmarsh habitats. Killifish were exposed to control (0 ng/L), low (17.8 ng/L), and high (596 ng/L) fluoxetine concentrations and subsequently tested in the presence …
Enhancing Walleye (Ogaa) Recruitment: Evaluating Assisted Reproduction As A Mitigation Strategy, Douglass Keiser
Enhancing Walleye (Ogaa) Recruitment: Evaluating Assisted Reproduction As A Mitigation Strategy, Douglass Keiser
Biology Graduate Theses
Over the past several decades, cold- and cool-water fish populations across the Upper Midwest, including Minnesota and Wisconsin, have shown troubling signs of decline. Among the most affected is the walleye/Ogaa (Sander vitreus), a species deeply embedded in the ecological balance, cultural identity, and economic vitality of the region, and central to the lifeways, treaty-reserved rights, and resource stewardship of Tribal Nations, including Ojibwe communities across the Upper Midwest. Despite ongoing stocking efforts and harvest regulations, many lake systems have experienced persistent declines in natural recruitment, prompting resource agencies to investigate the underlying causes. One such system is …
Enhancing Urban Garden Capacity By Linking Biodiversity And Ecosystem Function, Mary T. Nguyen, Carleen Rauh, Shelby Gresch, Charlotte Correiro, Heidi Elmendorf, Adriane Fugh-Berman, Gina Wimp, Alexandra L. Decandia
Enhancing Urban Garden Capacity By Linking Biodiversity And Ecosystem Function, Mary T. Nguyen, Carleen Rauh, Shelby Gresch, Charlotte Correiro, Heidi Elmendorf, Adriane Fugh-Berman, Gina Wimp, Alexandra L. Decandia
Virginia Journal of Science
Urban agriculture broadly describes food and plant cultivation in metropolitan areas. While biodiversity is thought to support ecosystem health and resilience, the relationship between above-ground and below-ground diversity has been understudied in urban green spaces. This study examines plant, animal, bacterial, and fungal diversity across three distinct urban habitats: a turf lawn, a forb garden, and a farming space. Using bacterial 16S rRNA and fungal ITS2 sequencing, soil nutrient analyses, and surveys of vegetation and wildlife, we connect diverse philosophies in urban agriculture to their role in advancing community diversity and ecosystem function. We found that different urban gardens support …
Microplastic-Mediated Delivery Of Di-Butyl Phthalate Alters C. Elegans Lifespan And Reproductive Fidelity, Chiara Angelyn Maldonado
Microplastic-Mediated Delivery Of Di-Butyl Phthalate Alters C. Elegans Lifespan And Reproductive Fidelity, Chiara Angelyn Maldonado
Presentations - 2026
•The challenge: microplastic contamination •The question: How do microplastics and chemical contaminants affect living organisms? •The follow-up: What’s next!
Trophic Structure And Mercury Bioaccumulation In Walleye And Yellow Perch In The Upper And Lower Red Lake Basins, Marissa Pribyl
Trophic Structure And Mercury Bioaccumulation In Walleye And Yellow Perch In The Upper And Lower Red Lake Basins, Marissa Pribyl
Biology Graduate Theses
Mercury is a persistent global contaminant that biomagnifies through aquatic food webs, with dietary and environmental factors serving as the primary drivers of accumulation in fishes. Trophic structure and methylmercury dynamics in Walleye (ogaa; Sander vitreus) and Yellow Perch (asaawens; Perca flavescens) were investigated in the Upper and Lower Red Lake basins in Red Lake, Minnesota, during 2024-2025. Diets of Walleye and Yellow Perch were assessed through stomach dissections, and tissue samples from both species were analyzed for total mercury concentrations. Additional analyses included shiners (gigoozens; Notropis spp., Hudsonius spp.) along with a variety of freshwater fish and …
Temporal Variation Of Benthic Aquatic Macroinvertebrate Communities In Two Upland Intermittent Streams In The Boston Mountains, R. J. Mccollough, K. B. Hecke
Temporal Variation Of Benthic Aquatic Macroinvertebrate Communities In Two Upland Intermittent Streams In The Boston Mountains, R. J. Mccollough, K. B. Hecke
Journal of the Arkansas Academy of Science
Benthic aquatic macroinvertebrates are some of the most highly used organisms for biomonitoring and assessing stream health. However, biomonitoring is often done in one season, potentially missing important taxa and inaccurately reflecting the community as well as stream health. In addition, while road crossing effects have also been extensively researched the last few decades, these studies are also often conducted in one season, likely misrepresenting the community structure and the overall effects of the road crossing. The highly known River Continuum Concept (RCC) is often applied to these benthic aquatic macroinvertebrate communities. This study aimed to examine aquatic macroinvertebrate communities …
Evaluating Sampling Strategies To Monitor Southern Fox Squirrel (Sciurus Niger Niger) Populations, Casey Robin Hitchens
Evaluating Sampling Strategies To Monitor Southern Fox Squirrel (Sciurus Niger Niger) Populations, Casey Robin Hitchens
Theses, Dissertations and Capstones
Reliable population monitoring is essential for evaluating wildlife translocations and informing conservation management, yet traditional monitoring approaches are often labor intensive and require prolonged sampling. This study evaluated marking techniques, survey methods, and population estimation approaches for monitoring a translocated population of southern fox squirrels (Sciurus niger niger) at Marine Corps Recruit Depot Parris Island, South Carolina, USA (MCRDPI). From January-June 2023, I conducted six live trapping and camera trapping sessions across five survey sites. I compared the performance of color-coded collars and tail shaves for individual identification and the recapture probability between live traps and camera traps. Logistic mixed-effects …
Neonicotinoid Bioaccumulation In Bluegill And Bullhead Within Lake Julia And Lake Zumwalde, Jake Stuber
Neonicotinoid Bioaccumulation In Bluegill And Bullhead Within Lake Julia And Lake Zumwalde, Jake Stuber
Journal of Earth and Life Science
Brown Bullhead Ameiurus nebulosus, Yellow Bullhead Ameiurus natalis, and Bluegill Lepomis macrochirus are unique in their stark differences. Bullhead are scaleless bottom feeders that can tolerate high pollution and low O2 conditions. In contrast, Bluegill are scaled, aggressive nesters and are less tolerant of high pollutants or low-oxygen conditions. Among these pollutants include a class of chemicals called neonicotinoids (hereafter referred to as neonics). Neonics are a class of insecticides primarily used within agriculture and pest management. Due to the effectiveness, application has escalated on a global scale. They have been well documented by many toxicology assessments. …
Mixture Effects On Metal Accumulation In The Asian Clam, Corbicula Fluminea, Mark A. Huff
Mixture Effects On Metal Accumulation In The Asian Clam, Corbicula Fluminea, Mark A. Huff
Electronic Theses and Dissertations
Field collected Corbicula fluminea were exposed to trace level copper, lead, and zinc with the aim of examining the preferential accumulation between the soft tissue and shell. Metal levels were based on those in a permitted industrial effluent. At 1X effluent and 2X effluent exposure levels, copper was found to preferentially accumulate in the clam soft tissue, lead was found to preferentially accumulate in the clam shell, and zinc was found to excrete back into the diluent after preferentially accumulating in the clam soft tissue. When exposed as a mixture at 1X effluent levels, copper and lead were found to …
From Rifles To Running Shoes: How Outdoor Recreation Trends Are Reshaping Conservation Efforts, Lane James Arthur
From Rifles To Running Shoes: How Outdoor Recreation Trends Are Reshaping Conservation Efforts, Lane James Arthur
All Graduate Theses and Dissertations, Fall 2023 to Present
Parks and protected areas (PPAs) serve a dual purpose: they conserve wildlife and natural habitats while providing spaces for people to enjoy outdoor recreation. However, balancing conservation with recreation can be challenging, especially as outdoor activities continue to grow and evolve. In Utah, Wildlife Management Areas (WMAs) are set aside to protect important wildlife habitats and provide opportunities for hunting and fishing. Recently, these areas have seen an increase in activities such as mountain biking, trail running, and dog walking, some of which take place in areas which prohibit recreational activity in effort to protect wildlife. These shifts in recreation …
Living Heritage, Resilience And Ethically Informed Infrastructures In Malawi, Martha B. Lerski, Frank Puluputu Mwale
Living Heritage, Resilience And Ethically Informed Infrastructures In Malawi, Martha B. Lerski, Frank Puluputu Mwale
Publications and Research
Rooted in a rural community’s needs, this paper explores how historically, and ethically informed infrastructures can support community engagement towards identification, documentation, and dissemination of local, traditional, and indigenous knowledge relating to climate change. The paper examines climate adaptation and sustainability issues through the lenses of community organizing and Library & Information Science best practices. It draws on a growing body of literature identifying a role for expanded epistemological and subject discipline perspectives. While the impacts of climate on material culture are increasingly recognized, intangible, and biocultural heritage have not received as much attention. Living heritage traditions and knowledge systems …
Plastic In The Pack: Assessing Microplastic Risk To Urban Coyotes (Canis Latrans) In The San Francisco Bay Area, Elaina E. Herrmann
Plastic In The Pack: Assessing Microplastic Risk To Urban Coyotes (Canis Latrans) In The San Francisco Bay Area, Elaina E. Herrmann
Master's Projects and Capstones
Microplastics are an emerging concern worldwide. However, their impact on terrestrial mammals, such as Canis latrans (coyotes), has yet to be extensively studied. Coyotes are unique in their ability to adapt to urban environments and have recently begun bridging the gap between marine and terrestrial food webs. This research examines the potential sources and impact of microplastic exposure in urban coyotes in the San Francisco Bay Area and possible food web impacts through literature synthesis and Geographic Information System (GIS) mapping. This research examined potential mechanisms for direct and trophic microplastic uptake by coyotes resulting from the anthropogenic, terrestrial, and …
Microbial Biodiversity Of The James River Rock Pool Ecosystem., Jordan C. Heydt
Microbial Biodiversity Of The James River Rock Pool Ecosystem., Jordan C. Heydt
Masters Theses, 2020-current
Rock pools are ephemeral aquatic environments that offer a unique opportunity to study complex communities and their responses to environmental changes. The urban James River rock pool system consists of over 700 individual rock pools varying in size and depth. This unique urban freshwater ecosystem has been studied for its invertebrate, plant, and zooplankton populations but not for its microbial community composition. To fill that gap, the microbial community of this system was characterized using 16S rRNA gene sequencing, and the influence of environmental variables on the community composition was analyzed. Our findings show variation in microbial community composition across …
Impact Of Unconventional Gas Extraction On Tributaries Of Tenmile Creek, Greene County, Pennsylvania: A 12-Year Perspective, Christian L. Beall
Impact Of Unconventional Gas Extraction On Tributaries Of Tenmile Creek, Greene County, Pennsylvania: A 12-Year Perspective, Christian L. Beall
Electronic Theses and Dissertations
Much of southwestern Pennsylvania has been central to a rapid increase in development for unconventional natural gas extraction in the past two decades. The continued monitoring of streams over their lifetime as extraction sites is pivotal to understanding the effects of fracking on local waterways and their ability to provide for wildlife and human use. Here, using multiple indices of biotic integrity (IBI), I perform analyses of fish and macroinvertebrate communities as well as water chemistry on two streams, Bates Fork (with shale wells) and Fonner Run (without), as part of a follow-up to work performed there from 2010-2012. Fish …
Investigating Drivers In Phytoplankton Between Lentic And Lotic Ecosystems Within Twelvepole Creek Watershed, Devin Lee Farmer
Investigating Drivers In Phytoplankton Between Lentic And Lotic Ecosystems Within Twelvepole Creek Watershed, Devin Lee Farmer
Theses, Dissertations and Capstones
Harmful Algal Blooms (HABs) threaten aquatic ecosystems, local economies, and water quality. While extensively studied in lakes (lentic systems), their impact on flowing waters (lotic systems) remains underexplored. This study examined algal drivers in both ecosystems within the same watershed, focusing on Beech Fork Lake (lentic) and Twelve Pole Creek (lotic). Key environmental factors, including pH, turbidity, dissolved oxygen, and nutrient levels, were measured using in situ sensors and laboratory analysis. Water samples were analyzed for nitrogen, phosphorus, and algal biomass for comparison to environmental conditions. Phytoplankton was preserved for identification in future research. By comparing these environments, this study …
Ms Environmental Biology Capstone: Evaluating Environmental Risks And Conflicts In Golf Course Landscapes, Meredith Boos
Ms Environmental Biology Capstone: Evaluating Environmental Risks And Conflicts In Golf Course Landscapes, Meredith Boos
Regis University Student Publications (comprehensive collection)
1) This review investigates the relationship between pesticide use on golf courses and in agriculture to determine whether the adverse health effects documented in agricultural settings also apply to golf course environments. While the negative health effects from agricultural pesticide use are well established, golf courses use pesticides with greater frequency and intensity, suggesting a potentially higher exposure risk for both workers and players.
2) This study proposes methods to quantify pesticide bioaccumulation on golf courses and adjacent lands. We hypothesize that areas surrounding golf courses will exhibit a spatial gradient of pesticide accumulation, increasing with proximity to treated sites …
Aquatic Invertebrate Populations In Roadside Wetlands In Relation To Water Characteristics, Ellen P. Isakson
Aquatic Invertebrate Populations In Roadside Wetlands In Relation To Water Characteristics, Ellen P. Isakson
Journal of Earth and Life Science
The continual habitat destruction and draining of wetland systems makes the need to understand the ecology of roadside wetlands all the more necessary. Roadside wetlands provide valuable habitat to countless aquatic invertebrates, which can provide insight into the health of the wetland system due to their diversity. The objective of this study was to analyze the populations of aquatic invertebrates in roadside wetlands in relation to water characteristics such as pH, salinity, conductivity, water depth and temperature, and dissolved oxygen. This was done by collecting samples of invertebrate populations from roadside wetlands, in addition to data on water quality characteristics …
Oyster Horizons: Spatial Prioritization For Oyster Restoration At Alabama Living Shorelines, Trinity Henry-Jack, Aaron Bland, Christa Russell, Ronald Baker
Oyster Horizons: Spatial Prioritization For Oyster Restoration At Alabama Living Shorelines, Trinity Henry-Jack, Aaron Bland, Christa Russell, Ronald Baker
Gulf and Caribbean Research
No abstract provided.
The Relationship Between Plant Density And Microplastic Concentration In The Water In Lake Bemidji, Mn, Arian Garcia
The Relationship Between Plant Density And Microplastic Concentration In The Water In Lake Bemidji, Mn, Arian Garcia
Journal of Earth and Life Science
Microplastics, plastic particles smaller than 5 mm, are an increasing environmental threat, particularly due to their widespread distribution and toxicity. While much research has focused on larger freshwater and marine ecosystems, the presence and impacts of microplastics in smaller inland lakes remain less understood. This study investigates the relationship between plant density and microplastic concentrations in Lake Bemidji, a smaller freshwater lake. A total of 30 water samples were collected across varying vegetation conditions, ranging from open water (0%) to 90% plant coverage. Microplastics were identified and categorized into six morphological types: fibers, fragments, films, spheres/beads, and foam. The samples …
Effects Of Round-Up On The Environment, Sandra J. Marcu
Effects Of Round-Up On The Environment, Sandra J. Marcu
Journal of Earth and Life Science
Many people around the world have used and still currently use Roundup but are unaware of the effects it has on the environment. Roundup is a spray on application weedkiller that is widely used around the world today both residentially and commercially. It enables its user to grow a garden or a field of crops with a no-tilling approach to eliminate weeds. It is a well-known and popular choice for killing weeds that has been around since the mid 1970’s (Oca, 2017). John Franz, a Monsanto scientist discovered that glyphosate (main ingredient in Roundup) was an herbicide or weedkiller, and …
The Effectiveness Of The Minnesota Buffer Law And Its Impacts On People, Ellen P. Isakson
The Effectiveness Of The Minnesota Buffer Law And Its Impacts On People, Ellen P. Isakson
Journal of Earth and Life Science
In January 2015, the then-governor of Minnesota Mark Dayton announced new legislation that would require 50-foot vegetation buffers along all waterways in Minnesota; this legislation would come to be known as the Minnesota buffer law, exact requirements being “perennial vegetative buffers of up to 50 feet along lakes, rivers, and streams and buffers of 16.5 feet along ditches” (Albert, 2017; MN Board of Water and Soil Resources [MN BWSR] 4 ). A buffer in the context of vegetation and natural resource management is a strip of vegetation around a body of water “designed to intercept stormwater runoff and minimize soil …
The Effects Of Salinity On The Cutaneous Microbiome And Pathogen Infection On The Pacific Tree Frog (Pseudacris Regilla) In Monterey Bay, California, Usa, Hannah Eunhae Kim
The Effects Of Salinity On The Cutaneous Microbiome And Pathogen Infection On The Pacific Tree Frog (Pseudacris Regilla) In Monterey Bay, California, Usa, Hannah Eunhae Kim
EWU Masters Thesis Collection
Global climate change and infectious diseases are considered a leading cause of amphibian population declines worldwide. Changes in precipitation and salinity have altered watershed systems and microbial communities, reducing habitat availability and physiological performances for amphibian survival. The salt refuge hypothesis, however, is a proposed explanation that higher saline environments may reduce susceptibility to pathogens such as Batrachochytrium dendrobatidis (Bd). The Moro Cojo Slough State Marine Reserve (MCSSMR) (California, USA) is known as an abundant breeding ground for endangered/threatened amphibian species. To test and expand the salt refuge hypothesis, I evaluated Bd prevalence and intensity and the cutaneous microbiome of …
Wildlife Distribution Mapping And Analysis In Gorongosa National Park, Mozambique, Using Site And Satellite Imagery Data, Pinho Joaquim Munhequeira Mr
Wildlife Distribution Mapping And Analysis In Gorongosa National Park, Mozambique, Using Site And Satellite Imagery Data, Pinho Joaquim Munhequeira Mr
Murray State Theses and Dissertations
One of Mozambique's most important conservation sites, Gorongosa National Park has seen critical ecological changes over the past several decades resulting from climate variability, habitat loss, and political instability. This research integrates Geographic Information Systems (GIS) and remote sensing to examine the relationship between vegetation dynamics and wildlife population, and distribution. The main goals of this study are to evaluate the spatial distribution of particular wildlife species: buffalo (Syncerus caffer), elephant (Loxodonta africana), hippopotamus (Hippopotamus amphibius), and zebra (Equus quagga), and determine the impact of vegetation density and land cover on their …
Investigating The Acute Physiological Effects Of Effluent Crystal Violet On The Freshwater Bioindicator Daphnia Pulex, Hoang K. Ho
Investigating The Acute Physiological Effects Of Effluent Crystal Violet On The Freshwater Bioindicator Daphnia Pulex, Hoang K. Ho
2024 Fall Honors Capstone Projects - Archive
This study examines the effects of acute exposure to crystal violet (CV) on the freshwater zooplankton Daphnia pulex in response to growing concerns over synthetic dyes from industrial runoff in aquatic ecosystems. The primary objective is to assess CV's impact on D. pulex's heart rate, mobility, morphology, and lethality via a series of acute exposure tests. 48-h acute toxicity (0-2000 μg/L), 48-h immobilization (0-180 μg/L), and 9-h heart rate (0-2,000 μg/L) tests were conducted using third-generation daphnids, followed by stereomicroscopic examination and statistical analysis. Results showed increasing mortality and decreasing mobility and heart rate with increasing CV concentration; the …
Emerging Contaminants In Extreme Environments: Microplastics Monitoring In Karst Springs Of Montenegro, Marko Nikolic, Djuradj Milosevic, Ana Manovic, Vladimir Pesic, Melita Mustafic, Dragana Asceric, Milica Nikolic, Dimitrija Savic-Zdravkovic
Emerging Contaminants In Extreme Environments: Microplastics Monitoring In Karst Springs Of Montenegro, Marko Nikolic, Djuradj Milosevic, Ana Manovic, Vladimir Pesic, Melita Mustafic, Dragana Asceric, Milica Nikolic, Dimitrija Savic-Zdravkovic
FRONTIERS UNBOUND: Exploring Extreme Environments
No abstract provided.
From Turf To Turf: Fungal Invasion Of Ascocoryne Turficola In A Northern Michigan Bog, Evan L. Coscarelli-Trott
From Turf To Turf: Fungal Invasion Of Ascocoryne Turficola In A Northern Michigan Bog, Evan L. Coscarelli-Trott
All NMU Master's Theses
This is the first paper to report Ascocoryne turficola Boud. 1905 (Helotiales: Ascomycota) within the United States. It is an obligate bog fungus native to Europe and Siberia, where it is extremely rare across most of its range. A. turficola appeared at Lily Pond Bog in Marquette, Michigan (87.47º W, 46.59º N) in 2019, almost contemporaneous with anecdotal reports in Minnesota and Ontario. Since 2019, the Lily Pond population has exploded and become the dominant fungus in this bog but has yet to spread to similar bogs nearby. This species is adapted to long distance dispersal and carries the potential …
Microplastic Accumulation In The Marsh Periwinkle (Littoraria Irrorata), Sarah Keaton, Hannah Lukhard, Eli Windy, Manar Hasan
Microplastic Accumulation In The Marsh Periwinkle (Littoraria Irrorata), Sarah Keaton, Hannah Lukhard, Eli Windy, Manar Hasan
DePaul Discoveries
Contamination of oceans by microplastics (mm) currently poses a major threat to aquatic and terrestrial ecosystems worldwide. Recent attention towards this issue has raised questions about the extent to which microplastics have accumulated in the environment, and has led to an increase in studies on the effects of microplastics in various organisms. However, levels of contamination in protected natural areas are still largely unexplored, yet can offer an important empirical perspective on the issue. In addition, little is known about the potential effects of microplastics on behavior in the field. This research was conducted within the protected ACE Basin National …