Pharmacology, Toxicology and Environmental Health Commons^™

Parthenolide Blocks Cocaine’S Effect On Spontaneous Firing Activity Of Dopaminergic Neurons In The Ventral Tegmental Area, David Schwarz, Damaris Bloom, Rocío Castro, Oné R. Pagán, C. A. Jiménez-Rivera

Biology Faculty Publications

Chronic cocaine administration leads to catecholamine reuptake inhibition which enhances reward and motivational behaviors. Ventral Tegmental Area dopaminergic (VTA DA) neuronal firing is associated with changes in reward predictive signals. Acute cocaine injections inhibit putative VTA DA cell firing in vertebrates. Parthenolide, a compound isolated from the feverfew plant (Tanacetum parthenium), has been shown to substantially inhibit cocaine’s locomotion effects in a planarian animal model (Pagán et al., 2008). Here we investigated the effects of parthenolide on the spontaneous firing activity of putative VTA DA neurons in anesthetized male rats (250-300g). Single-unit recordings were analyzed after intravenous (i.v.) parthenolide administration …

Specific Thiazolidinediones Inhibit Ovarian Cancer Cell Line Proliferation And Cause Cell Cycle Arrest In A Pparγ Independent Manner, Linah Al-Alem, R. Chase Southard, Michael W. Kilgore, Thomas E. Curry

Pharmacology and Nutritional Sciences Faculty Publications

BACKGROUND: Peroxisome Proliferator Activated Receptor gamma (PPARγ) agonists, such as the thiazolinediones (TZDs), have been studied for their potential use as cancer therapeutic agents. We investigated the effect of four TZDs--Rosiglitazone (Rosi), Ciglitazone (CGZ), Troglitazone (TGZ), and Pioglitazone (Pio)--on ovarian cancer cell proliferation, PPARγ expression and PPAR luciferase reporter activity. We explored whether TZDs act in a PPARγ dependent or independent manner by utilizing molecular approaches to inhibit or overexpress PPARγ activity.

PRINCIPAL FINDINGS: Treatment with CGZ or TGZ for 24 hours decreased proliferation in three ovarian cancer cell lines, Ovcar3, CaOv3, and Skov3, whereas Rosi and Pio had no …

National Center For Pharmaceutical Crops, Wei Yuan, Shiyou Li, Zushang Su, Ping Wang

NCPC Presentations

The mission of the National Center for Pharmaceutical Crops is to develop crops for the production of pharmaceutical and other bioactive compounds. The NCPC, located in the Arthur Temple College of Forestry and Agriculture, is the only one of its kind in the U.S. and it seeks to improve human health, revitalize rural economies and enhance U.S. security in strategic pharmaceuticals. This poster provides a background to the department, its functions and activities and notable achievements.

Evaluating The Effectiveness Of A Commercial Portable Air Purifier In Homes With Wood Burning Stoves: A Preliminary Study, Julie Hart, Tony Ward, Terry Spear, Richard Rossi, Ph.D., Nicolas Holland M, Brodie Loushin

Safety Health & Industrial Hygiene

Wood burning for residential heating is prevalent in the Rocky Mountain regions of the United States. Studies have shown that wood stoves can be a significant source of PM2.5 within homes. In this study, the effectiveness of an electrostatic filter portable air purifier was evaluated (1) in a home where a wood stove was the sole heat source and (2) in a home where a wood stove was used as a supplemental heat source. Particle count concentrations in six particle sizes and particle mass concentrations in two particle sizes were measured for ten 12-hour purifier on and ten purifier off …

Estrogen Sulfotransferase (Sult1e1) Expression And Function In Mcf10a-Series Breast Epithelial Cells: Role As A Modifier Of Breast Carcinogenesis And Regulation By Proliferation State, Jiaqi Fu

Wayne State University Dissertations

Estrogen sulfotransferase (SULT1E1) catalyzes the sulfonation of estrogens, which limits estrogen mitogenicity. TaqMan Gene Expression assays were used to profile the mRNA expression of estrogen receptor (ERα and ERβ) and estrogen metabolism enzymes including cytosolic sulfotransferases (SULT1E1, SULT1A1, SULT2A1, and SULT2B1), steroid sulfatase (STS), aromatase (CYP19), 17β-hydroxysteroid dehydrogenases (17βHSD1 and 2), CYP1B1, and catechol-O-methyltransferase (COMT) in an MCF10A-derived lineage cell culture model for basal-like human breast cancer progression and in ERα-positive luminal MCF7 breast cancer cells. Low levels of ERα and ERβ mRNA were present in MCF10A-derived cell lines. SULT1E1 mRNA was more abundant in confluent relative to subconfluent MCF10A …

Hedgehog Signaling: A Potential Therapeutic Target For Non-Small Cell Lung Cancer, Ma'in Yehya Maitah

Wayne State University Dissertations

The American Cancer Society estimated that 222,520 Americans were diagnosed with lung cancer and 157,300 died of lung cancer in 2010 (Jemal et al. 2009, 225-249;Jemal et al. 2011, 69-90). The clinical outcome of patients diagnosed with non-small cell lung cancer (NSCLC), the major lung cancer sub-types, is very poor, which calls for innovative research for finding novel therapeutic targets and agents for better treatment outcome.

Emerging evidences have suggested that a phenomenon called Epithelial-to-Mesenchymal Transition (EMT), which shares similar molecular characteristics with cancer stem-like cells, contributes to lung cancer treatment failure. In view of the fact that EMT process …

Full Issue: Volume 4, Number 2

The Science Journal of the Lander College of Arts and Sciences

No abstract provided.

Compensatory Renal Hypertrophy, Mitochondria And Redox Status, Bavneet Benipal

Wayne State University Dissertations

A reduction in functional renal mass occurs in humans during aging and severe kidney damage from diseases, injuries, infections and congenital conditions and after nephrectomy. Nephrectomy, or surgical removal of a kidney or a section of a kidney, is performed for treatment of unilateral secondary renal cancer, infections and for kidney transplantation. As a result, the remaining renal tissue undergoes compensatory growth due primarily to hypertrophy, in which both the size and functional capacity of the remaining kidney are increased. Renal compensatory hypertrophy is associated with a series of physiological, morphological and biochemical changes that also have toxicological implications.

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Adding Value To Ecological Risk Assessment With Population Modeling, Valery E. Forbes, Peter Calow, Volker Grimm, Takehiko I, Hayashi, Tjalling Jager, Agnete Katholm, Annemette Palmqvist, Rob Pastorok, Dan Salvito, Richard Sibly, Julann Spromberg, John Stark, Richard A. Stillman

Valery Forbes Publications

Current measures used to estimate the risks of toxic chemicals are not relevant to the goals of the environmental protection process, and thus ecological risk assessment (ERA) is not used as extensively as it should be as a basis for cost-effective management of environmental resources. Appropriate population models can provide a powerful basis for expressing ecological risks that better inform the environmental management process and thus that are more likely to be used by managers. Here we provide at least five reasons why population modeling should play an important role in bridging the gap between what we measure and what …

Effects Of Marmin Isolated From Aegle Marmelos Correa On L-Histidine Decarboxylase Enzyme In Rbl-2h3 Cells, Agung Endro Nugroho, Novrizal Abdi, Sugeng Riyanto, Kazutaka Maeyama, Zullies Ikawati

The Thai Journal of Pharmaceutical Sciences

Marmin or (7-(6’,7’-dihydroxygeranyl-oxy) coumarin is a compound isolated from Aegle marmelos Correa, in the study, we investigated the effects of marmin on the L-histidine decarboxylase (HDC), a rate-limiting histamine synthesizing enzyme, in rat ma...

Rapid Titrimetric And Spectrophotometric Assay Of Tramadol In Bulk Drug And In Formulation Using N-Bromosuccinimide And Methyl Orange As Reagents, K. B. Vinay, H. D., N. Rajendraprasad, M. S. Raghu, P. J. Ramesh, M. X. Cijo, K. Basavaiah

The Thai Journal of Pharmaceutical Sciences

Tramadol is a centrally acting analgesic used for prevention and treatment of moderate to severe pain. Two simple, rapid and sensitive methods by titrimetric and spectrophotometric techniques are described for the determination of tramadol hydrochloride ...

Allylmercaptocaptopril Attenuates Sodium Selenite Induced Experimental Cataractogenesis: An In Vitro And In Vivo Study, K. S. Lakshmi, Shrinivas Sharma

The Thai Journal of Pharmaceutical Sciences

The present study sought to assess the efficacy of the allylmercaptocaptopril เท preventing seleniteinduced cataractogenesis in an experimental setting. The first, in vitro phase of the study was performed on lenses from Wistar rats incubated for 24 ...

Design And Characterization Of Bioadhesive Ophthalmic Films Of Flurbiprofen, Rashi Saroot, Ritu Mehra Gilhotra

The Thai Journal of Pharmaceutical Sciences

An attempt has been made to formulate sustained release ophthalmic films of flurbiprofen along with bioadhesive polymers. Ocular films were prepared by using polymers; sodium alginate, chitosan and HPMC; alone and in different combinations. Glycerol was ...

Influence Of Surfactants On Crystal Form Of Mefenamic Acid, Kaival P., Kulkarni P.K., Mudit Dixit, Ashwini G. Kni

The Thai Journal of Pharmaceutical Sciences

Mefenamic acid is an anti inflammatory and poorly water-soluble drug. Since the drug powder can stick to any type of surface, and is not easy to handle in granulation and tabletting process, it is more desirable to recrystallize the mefenamic acid in suc...

Acute And Sub-Chronic Toxicity Studies Of A Standardized Extract Of Centella Asiatica Eca 233, Songpol Chivapat, Pranee Chavalittumrong, Mayuree H. Tantisira

The Thai Journal of Pharmaceutical Sciences

Acute toxicity study of Centella asiatica standardized extract ECa 233 was conducted by an oral administration of 10.0 g/kg extract into 10 male and 10 female mice. The extract at the given dose did not cause any toxic signs and death within the observat...

Hptlc-Densitometric Method Development And Validation For Simultaneous Determination Of Lamivudine, Nevirapine And Zidovudine In Fixed Dose Combinations, Gebremedhin Solomon, Ariaya Hymete, Abdel-Maaboud I. Mohamed, Adnan A. Bekhit

The Thai Journal of Pharmaceutical Sciences

In this study an HPTLC-densitometric method was developed for the simultaneous determination of lamivudine, nevirapine and zidovudine in pharmaceutical dosage forms which are used widely as first line HIV/AIDS treatment. Chromatographic separation of the...

Ion-Pair Titrimetric Assays Of Quetiapine Fumarate In Pharmaceuticals Using Sodium Tetraphenylboron And Sodium Lauryl Sulphate, N. Rajendraprasad, K. Basavaiah, Kanakapura B. Vinay

The Thai Journal of Pharmaceutical Sciences

Two simple, rapid, precise and accurate titrim etric methods employing sodium tetraphenylboron (STPB) and sodium lauryl sulphate (SLS) as titrants are described for the quantitative determination of quetiapine fumarate (QTF) in bulk drug and tablets. The...

Simultaneous Determination Of Ampicillin Sodium And Sulbactam Sodium In Binary Mixtures And Commercial Dosage Forms Using Chemometrics - Assisted Spectrophotometric Techniques, Zelalem M, Abdel-Maaboud I., Adnan A. Bekhit, Ariaya Hymete

The Thai Journal of Pharmaceutical Sciences

The binary mixture of ampicillin sodium and sulbactam sodium is officially analyzed by HPLC but can not be simultaneously determined by the normal spectrophotometric techniques since their absorption spectra have a strong degree of overlapping. The curre...

Minocycline And Magnesium In Combination Attenuates Oxidative Stress And Decreases Ischemic Neuronal Damage In Vivo, Pallab Bhattacharya, Anand Kr., Sudip Paul

The Thai Journal of Pharmaceutical Sciences

A neuroprotective strategy through a combination therapy is always being superior to any other singular therapeutic interventions, as these acts through a multifaceted approach within the brain during focal cerebral ischemia. Therefore, the development o...

Use Of Bromate - Bromide Mixture As A Reagent For The Determination Of Dothiepin Hydrochloride In Pharmaceuticals, Sameer A.M. Abdulrahman, Kanakapura Basavaiah

The Thai Journal of Pharmaceutical Sciences

The present study describes one titrimetric and two spectrophotometric methods for the determination of dothiepin hydrochloride (DOTH) in bulk drug and in tablets. The methods employed bromate-bromide mixture in acid medium as an oxidimetric as well as b...

Antioxidant Properties Of Squeezed Mangosteen Juice, Monrudee Sukma, Tanasait Ngawhirunpat, Praneet Opanasopit, Pongpun Siripong

The Thai Journal of Pharmaceutical Sciences

The aim of this study was to investigate antioxidant effects of squeezed mangosteen (Garcinia mangostana) juice as preferable diet for human consumption. Mangosteen juice was prepared from the aril part. Antioxidant properties were evaluated by total ant...

Author Index, Keyword Index, N/A

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

Synthesis And Antihepatotoxic Activity Of Some New Xanthones Containing 1,4-Dioxane Ring System, Shah Alam Khan, Bahar Ahmed

The Thai Journal of Pharmaceutical Sciences

This study was aimed at the synthesis of few xanthone derivatives containing 1,4-dioxane ring system. The reaction of 3,4-dihydroxy xanthone derivatives (3a-c) with ethylene dibromide and epichlorohydrin under approriate reaction conditions afforded xant...

Effect Of Ocimum Sanctum Extract On Sodium Nitrite - Induced Experimental Amnesia In Mice, Manoj Dokania, Kamal Kishore, P.K. Sharma

The Thai Journal of Pharmaceutical Sciences

The present study investigated the antiamnesic effect of the extract of Ocimum sanctum (OS) on time induced amnesia and sodium nitrite-induced amnesia in mice by using two different rodent models of memory. The aqueous extract of OS was administered to m...

High Resolution Hybrid Mass Spectrometry For The Determination Of Marine Biotoxins, Azaspiracids, And Their Biotransformation Products., Zuzana Skrabakova

Theses

Azaspiracids (AZAs), polyether marine toxins, are produced by marine microalgae and accumulate in filter-feeding bivalve molluscs, such as mussels, clams, oysters and scallops, while grazing on phytoplankton, from which they derive nutrition. Although being discovered quite recently, AZAs have caused several human intoxication events and thus became a problem for health authorities and shellfish industries. The rapid development of highly sensitive liquid chromatography multiple tandem mass spectrometry (LC-MS/MS, LC-MS") methods enabled the identification of numerous AZA analogues and added to the control of shellfish contamination. Tandem mass spectrometry techniques, while being highly selective and sensitive for determination of AZAs in …

An Overview Of New Psychoactive Substances And The Outlets Supplying Them, Cathy Kelleher, Rachel Christie, Kevin Lalor, John Fox, Matt Bowden, Cora O'Donnell

Reports

This report represents the outcome of a review of new psychoactive substances2 within the Irish context, including a review of the outlets that supply these substances. The review was commissioned by the National Advisory Committee on Drugs (NACD) in accordance with Action 14 of the National Drugs Strategy (interim) 2009–2016. Action 14 provides for the monitoring of ‘head shops’3 and other outlets for the sale of psychoactive substances, under the Misuse of Drugs Act 1977 and the Misuse of Drugs (Amendment) Regulations 2007. Researchers at the Centre for Social and Educational Research (CSER) within the School of Social Sciences and …

Novel Inhibitors Of Lysine Specific Demethylase 1 As Epigenetic Modulators, Michael Crowley

Wayne State University Theses

The recently discovered enzyme lysine-specific demethylase 1 (LSD1) plays an important role in the epigenetic control of gene expression, and aberrant gene silencing secondary to LSD1 over expression is thought to contribute to the development of cancer. We recently reported a series of (bis)guanidines and (bis)biguanides that are potent inhibitors of LSD1, and induce the re-expression of aberrantly silenced tumor suppressor genes in tumor cells in vitro. We now report a new series of isosteres that are also potent inhibitors of LSD1. These compounds induce increases in methylation at the histone 3 lysine 4 (H3K4) chromatin mark, a specific target …

Gene And Environment Interactions Modulate Immune System Influences On Social Behavior, Yubin Zhang

Legacy Theses & Dissertations (2009 - 2024)

Autism is a neurodevelopmental disorder with unknown etiology, affecting about 1 in 110 children in the US. Here, we show that developmental exposure to anti-brain Abs impaired mouse social behaviors. BTBR mice, a mouse strain with high serum anti-brain Abs and behaviors that resemble autism, have more activated splenic B cells, CD4+ T cells (Th1, Th2 and Th17 subsets), and CD4+ T cells with expression of Vb6 chains than B6 mice (the control H-2 identical strain). Elevated numbers of activated (CD25+) CD4+Vb6+ cells also were detected in thymic preparations. All of the immunological and behavioral parameters of BTBR mice were …

The Role Of Androstane Neurosteroids In Alcohol-Mediated Social Behavior, Jason J. Paris

Legacy Theses & Dissertations (2009 - 2024)

Androgenic steroids, such as testosterone, may influence the propensity for aggression in both people and animals. Some of testosterone's effects on aggression may be due, in part, to its metabolic products that are 5á-reduced and 3á-hydroxylated to form, 3á-androstanediol (3á-diol), which can also enhance aggression in mice. Notably, alcohol (EtOH) consumption facilitates aggression in people and animals, particularly among those predisposed to act aggressively. In rats, EtOH can increase 3á-diol in prefrontal cortex, which may facilitate aggression. The present work aimed to elucidate the role of 3á-diol for EtOH-enhanced aggression. We hypothesized that EtOH would enhance inter-male aggression, social dominance, …

The Nasal Toxicity Of The Herbicide 2,6-Dichlorobenzonitrile : Mechanisms And Biomarkers, Fang Xie

Legacy Theses & Dissertations (2009 - 2024)

The overall goal of this study is to investigate mechanisms of the potent nasal specific toxicity of the herbicide 2,6-dichlorobenzonitrile (DCBN) in rodents, and to determine whether DCBN could induce similar nasal toxicity in humans. The central hypotheses are 1) that the nasal specific toxicity of DCBN in rodents is mediated by its electrophilic intermediates that are formed through metabolic activation catalyzed by target tissue cytochrome P450 enzymes (P450) and can interfere with stem cell regeneration and differentiation in the olfactory epithelium (OE); and 2) that human nasal tissues are also capable of catalyzing bioactivation of DCBN. The specific aims …