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Synthesis, Characterization And Manipulation Of Creighton Silver Nanoparticles For Future Cytotoxicity Studies, Sesha Lakshmi Paluri
Synthesis, Characterization And Manipulation Of Creighton Silver Nanoparticles For Future Cytotoxicity Studies, Sesha Lakshmi Paluri
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Nowadays, 24% of the nanomaterial-based consumer products contain silver nanoparticles (AgNPs) and exploit the well-known antimicrobial properties of silver. Although AgNPs have a wide range of biomedical research and industrial applications, very little is known about their toxicity. The main goal of this study is to synthesize, characterize and manipulate Creighton colloidal AgNPs for future cytotoxicity studies. These "naked" AgNPs were free from chemically aggressive capping/stabilizing agents, reaction byproducts or organic solvents. To achieve this goal, colloidal AgNPs were successfully: a) synthesized in large volumes (5L) using a slightly modified Creighton method by the reduction of silver nitrate with sodium …
In-Vitro Biocompatibility Of Silver Nanoparticles Anchored On Multi-Walled Carbon Nanotubes, Alicia Brooks Castle
In-Vitro Biocompatibility Of Silver Nanoparticles Anchored On Multi-Walled Carbon Nanotubes, Alicia Brooks Castle
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Silver (Ag) nanoparticles were anchored on the surface of three different multi-walled carbon nanotubes (MWNTs): Ag-MWNTs (pure carbon), Ag-MWNT-COx (carbonyl and carboxyl doped), and Ag-MWNT-CNx (Nitrogen-doped). The chemical reactivity of the nanotubes was MWNTs< COx-MWNTs< CNx-MWNT. Ag-NP anchored on the MWNTs had an average size corresponding to MWNTs 6.64nm +/- 2.25nm; CNx-MWNTs, 13.24nm +/- 3.94nm, and COx-MWNT/Ag 11.75nm +/- 4.65nm. Cellular function and immune response were evaluated to determine biocompatibility of the synthesized nanomaterials (NM) on the human keratinocyte cell line (HaCaT). Cellular assays revealed toxicity after 24h however, full cellular recovery was observed at 48h. These results suggest Ag nanoparticles anchored on MWNTs have the ability to interact with cells without disrupting cellular function.
In Vitro Toxicity Assessment Of Silver Nanoparticles In Rat Alveolar Macrophages, Cataleya Carlson
In Vitro Toxicity Assessment Of Silver Nanoparticles In Rat Alveolar Macrophages, Cataleya Carlson
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The present study was conducted to assess the toxicity of nanosized silver particles (Ag-15nm, 30nm and 55nm) in rat alveolar macrophages. For toxicity evaluations, cellular morphology, mitochondrial function (MTT assay), membrane leakage (LDH assay) and reactive oxygen species generation (ROS) were assessed after a 24h under control and exposed conditions. The morphological appearance of control and exposed cells were observed by an inverted, phase contrast microscope and the uptake of nanoparticles was observed using the CytoViva Ultra Resolution Imaging (URI) system. Morphological images captured at 100x magnification demonstrated that nanoparticle-exposed cells at higher doses became abnormal in size, and most …