Pharmacology, Toxicology and Environmental Health Commons^™

The Standardized Fish Bioassay Procedure For Detecting And Culturing Actively Toxic Pfiesteria, Used By Two Reference Laboratories For Atlantic And Gulf Coast States, Joann M. Burkholder, Harold G. Marshall, David W. Seaborn, Nora J. Deamer-Melia

Biological Sciences Faculty Publications

In the absence of purified standards of toxins from Pfiesteria species, appropriately conducted fish bioassays are the "gold standard" that must be used to detect toxic strains of Pfiesteria slop. from natural estuarine water or sediment samples and to culture actively toxic Pfiesteria. In this article, we describe the standardized steps of our fish bioassay as an abbreviated term for a procedure that includes two sets of trials with fish, following the Henle-Koch postulates modified for toxic rather than infectious agents. This procedure was developed in 1991, and has been refined over more than 12 years of experience in …

Casco Bay Watershed Wetlands Characterization, State Planning Office

Maine Collection

Casco Bay Watershed Wetlands Characterization

State of Maine Executive Department - State Planning Office

Augusta, Maine, May 2001

Contents: Abstract / Background / Getting Started / Designing the System / The Characterization / The Prioritization / Results / Cautions / Significance of the Approach / Literature Cited / Figure 1 - Biophysical Regions of Maine / Appendix A: Steering Committee Members / Appendix B: Field Verification Form / Appendix C: Contacts

Escherichia Coli O157:H7 In Free-Ranging Deer In Nebraska, David G. Renter, Jan M. Sargeant, Scott E. Hygnstrom, Jeff D. Hoffmann, Jerry R. Gillespie

School of Natural Resources: Faculty Publications

In order to determine the prevalence and distribution of the human pathogen, Escherichia coli O157:H7, in free-ranging deer, hunters were asked to collect and submit fecal samples from deer harvested during a regular firearm season (14–22 November 1998). Prior to the season, 47% of the hunters with permits in the southeastern Nebraska (USA) study area indicated a willingness to participate in the study. Approximately 25% of successful hunters in the area submitted deer fecal samples. Escherichia coli O157:H7 was cultured from four (0.25%) of 1,608 total samples submitted. All of the fecal samples that were properly identified (1,426) and all …

Effects Of Humic Acids And Salinity On Pesticide Bioavailability And Toxicity As Estimated By Spme And Toxicity Tests, Laurent C. Mézin

Dissertations, Theses, and Masters Projects

The interactive effects of humic acids (HAs) and salinity on the bioavailability and toxicity of the pesticides chlorpyrifos and 4,4'-dichlorodiphenyltrichloroethane (DDT) were investigated. The effects of various HAs on the toxicity of chlorpyrifos were initially assessed with the chronic MicrotoxRTM test. Environmentally relevant concentrations of Aldrich, Peat, Leonardite and Suwannee River HAs had no significant effect on the toxicity of either chlorpyrifos or copper (Cu), the test's positive control. as reductions in contaminant toxicity had previously been reported for some contaminants by HAs, it appeared that salinity might be a mitigating factor. Thus salinity effects were further investigated with Aldrich …

Heat Shock Protein (Hsp70) Response In The Eastern Oyster, Crassostrea Virginica, Exposed To Various Contaminants (Pahs, Pcbs And Cadmium), Luis A. Cruz Rodriguez

Dissertations, Theses, and Masters Projects

The stress protein response has been proposed as a general indicator of exposure to stress as their expression might integrate overall biological impact and interactions among multiple stressors. as a marker of contaminant effects, a major advantage is the premise of higher sensitivity over other indices such as condition index, scope for growth and survival. Laboratory exposure to suspended field contaminated sediments (SFCS) elicited a stress protein response (HSP70) in the eastern oyster, ( Crassostrea virginica). The stress response probably resulted from the combined effect of various contaminants including PAHs, metals and PCBs. Exposure to 1--2g suspended clay particles spiked …