Molecular, Genetic, and Biochemical Nutrition Commons^™

Regulation Of P53 Stability And Apoptosis By A Ror Agonist, Yongjun Wang, Laura A. Solt, Douglas J. Kojetin, Thomas P. Burris

Department of Nutrition and Health Sciences: Faculty Publications

Activation of p53 function leading to cell-cycle arrest and/or apoptosis is a promising strategy for development of anticancer therapeutic agents. Here, we describe a novel mechanism for stabilization of p53 protein expression via activation of the orphan nuclear receptor, RORa. We demonstrate that treatment of cancer cells with a newly described synthetic ROR agonist, SR1078, leads to p53 stabilization and induction of apoptosis. These data suggest that synthetic ROR agonists may hold utility in the treatment of cancer.

Regulation Of Expression Of Citrate Synthase By The Retinoic Acid Receptor-Related Orphan Receptor A (Rorα), Christine Crumbley, Yongjun Wang, Subhashis Banerjee, Thomas P. Burris

Department of Nutrition and Health Sciences: Faculty Publications

The retinoic acid receptor-related orphan receptor α (RORα) is a member of the nuclear receptor superfamily of transcription factors that plays an important role in regulation of the circadian rhythm and metabolism. Mice lacking a functional RORα display a range of metabolic abnormalities including decreased serum cholesterol and plasma triglycerides. Citrate synthase (CS) is a key enzyme of the citric acid cycle that provides energy for cellular function. Additionally, CS plays a critical role in providing citrate derived acetyl-CoA for lipogenesis and cholesterologenesis. Here, we identified a functional RORα response element (RORE) in the promoter of the CS gene. ChIP …

Effects Of Single Nucleotide Polymorphisms In The Human Holocarboxylase Synthetase Gene On Catalytic Activity, Shingo Esaki

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Holocarboxylase synthetase (HCS) catalyzes the covalent binding of biotin to carboxylases and histones in eukaryotic cells. Biotinylated carboxylases play essential roles in the metabolism of fatty acids, amino acids, and glucose; biotinylated histones play essential roles in gene regulation and genome stability. HCS null individuals are not viable whereas HCS deficiency is linked to developmental delays and phenotypes such as short life span and low stress resistance. Greater than 2,500 single nucleotide polymorphisms (SNPs) have been reported for HCS, but the biological importance of these polymorphisms is unknown. We hypothesized that some of these SNPs impair catalytic activity and that …

High-Density Lipoprotein Metabolism In Human Apolipoprotein B100 Transgenic/Brown Adipose Tissue Deficient Mice: A Model Of Obesity-Induced Hyperinsulinemia, Sarah J. Ehlers, Stephanie M. Larson, Heather E. Rasmussen, Young-Ki Park, Ji-Young Lee

Department of Nutrition and Health Sciences: Faculty Publications

Obese and diabetic humans display decreased plasma high-density lipoprotein cholesterol (HDL-C) concentrations and an increased risk for coronary heart disease. However, investigation on HDL metabolism in obesity with a particular emphasis on hepatic ATP-binding cassette transporter A1 (ABCA1), the primary factor for HDL formation, has not been well studied. Human apolipoprotein B₁₀₀ transgenic (hApoB^tg) and brown adipose tissue deficient (BATless) mice were crossed to generate hApoB^tg/BATless mice. Male and female hApoB^tg and hApoB^tg/BATless mice were maintained on either a regular rodent chow diet or a diet high …

Regulation Of Sterol Transport By Dietary Phytosterol Esters, Trevor J. Carden

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

LDL cholesterol is associated with the development of atherosclerosis and is therefore considered an important target for intervention to prevent cardiovascular diseases. The inhibition of cholesterol absorption in the small intestine is an attractive approach to lowering plasma cholesterol, one that is exploited by drug therapy as well as dietary supplementation with plant sterols. The mechanism of action of plant sterol esters (PSE) is still incompletely understood, therefore this study was conducted to test the hypothesis that hydrolysis of plant sterol esters is necessary for their cholesterol-lowering effects to be realized.

Male Syrian hamsters were fed diets containing no PSE, …

Unsaturated Fatty Acids Repress The Expression Of Atp-Binding Cassette Transporter A1 In Hepg2 And Fhs 74 Int Cells, Chai Siah Ku, Heather E. Rasmussen, Young-Ki Park, Elliot D. Jesch, Ji-Young Lee

Department of Nutrition and Health Sciences: Faculty Publications

Adenosine triphosphate–binding cassette transporter A1 (ABCA1) plays a critical role in the formation and metabolism of high-density lipoproteins (HDLs). Adenosine triphosphate–binding cassette transporter A1 in the liver and small intestine, in particular, accounts for approximately 90% of plasma HDL cholesterol. Therefore, any alterations in the hepatic and intestinal expression of ABCA1 could have a large impact on HDL biogenesis. We tested the hypothesis that ABCA1 expression is regulated differentially by different types of fatty acids in the liver and small intestine. Human hepatoma HepG2 and human small intestine epithelial FHs 74 Int cells were used as an in vitro model. …

Mechanisms Of Decreased Cholesterol Absorption Mediated By Phytosterols In The Intestinal Lumen, Andrew W. Brown

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Phytosterols and their fatty acyl esters have been known for decades to lower LDL cholesterol, making them powerful nutraceuticals in lowering cardiovascular disease risk. The mechanisms by which phytosterols lower cholesterol, though, have been incompletely characterized. Three studies were executed to examine three aspects of cholesterol and phytosterol interactions in the intestinal lumen. In the first study, the ability of pancreatic cholesterol esterase to hydrolyze phytosterol esters was examined. Pancreatic cholesterol esterase hydrolyzed phytosterol esters, but the rate of hydrolysis proved sensitive to the structures of both the sterol and ester components. In the second study, cholesterol micellarization was challenged …

Adipose Tissue Abca1 Contributes To Hdl Biogenesis In Vivo, Soonkyu Chung, Janet K. Sawyer, Abraham K. Gebre, Nobuyo Maeba, John S. Parks

Department of Nutrition and Health Sciences: Faculty Publications

Background—Adipose tissue (AT) is the body’s largest free cholesterol (FC) reservoir and abundantly expresses ATP binding cassette transporter A1 (ABCA1), a key cholesterol transporter for HDL biogenesis. However, the extent to which AT ABCA1 expression contributes to HDL biogenesis in vivo is unknown.

Methods and Results—Adipocyte-specific ABCA1 knockout mice (ABCA1^−A/−A) were

generated by crossing ABCA1floxed mice with aP2 cre transgenic mice. AT from ABCA1^−A/−A mice had −^A/−A mice had a twofold increase in FC content, compared to WT mice, and failed to efflux cholesterol to apoA-I. …

The Role Of Histone H4 Biotinylation In The Structure Of Nucleosomes, Nina A. Filenko, Carol Kolar, John T. West, S. Abbie Smith, Yousef I. Hassan, Gloria E. O. Borgstahl, Janos Zempleni, Yuri L. Lyubchenko

Department of Nutrition and Health Sciences: Faculty Publications

Background: Post-translational modifications of histones play important roles in regulating nucleosome structure and gene transcription. It has been shown that biotinylation of histone H4 at lysine-12 in histone H4 (K12Bio-H4) is associated with repression of a number of genes. We hypothesized that biotinylation modifies the physical structure of nucleosomes, and that biotin-induced conformational changes contribute to gene silencing associated with histone biotinylation.

Methodology/Principal Findings: To test this hypothesis we used atomic force microscopy to directly analyze structures of nucleosomes formed with biotin-modified and non-modified H4. The analysis of the AFM images revealed a 13% increase in the length of …

Body Mass Index Is Associated With Dietary Patterns And Health Conditions In Georgia Centenarians, Dorothy B. Hausman, Mary Ann Johnson, Adam Davey, Leonard W. Poon

Department of Nutrition and Health Sciences: Faculty Publications

Associations between body mass index (BMI) and dietary patterns and health conditions were explored in a population-based multiethnic sample of centenarians from northern Georgia. BMI ≤20 and ≥25 was prevalent in 30.9% and 25.3% of study participants, respectively. In a series of logistic regression analyses controlled for gender and place of residence, the probability of having BMI ≥25 was increased by being black versus white and having a low citrus fruit, noncitrus fruit, orange/yellow vegetable or total fruit and vegetable intake. The probability of having BMI ≤20 was not associated with dietary intake. When controlled for race, gender, residence, and …

Regulation Of Atp Binding Cassette Transporter A1 And G1 Expression By Fatty Acids, Chai Siah Ku

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

High density lipoprotein (HDL) is an atheroprotective element which is critical in the reverse cholesterol transport (RCT) pathway to eliminate excess cholesterol from the periphery of the body. A low plasma HDL cholesterol levels indicate a higher risk of getting cardiovascular diseases (CVD). Formation and metabolism of HDL largely rely on ATP binding cassette transporter A1 (ABCA1) and G1 (ABCG1) that are transporters to facilitate the efflux of cellular cholesterol to outside of cells. ABCA1 in the liver and small intestine is the major contributing factor for HDL formation. On the other hand, ABCA1 and ABCG1 present in macrophages contribute …

Holocarboxylase Synthetase-Dependent Biotinylation Of Histone H4, Luisa F. Rios Avila

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Holocarboxylase synthetase (HCS) catalyzes the binding of biotin to lysine (K) residues in histones H3 and H4. Histone biotinylation marks play important roles in the repression of genes and retrotransposons. Preliminary studies suggested that K16 in histone H4 is a target for biotinylation by HCS. Here we demonstrated that H4K16bio is overrepresented in repeat regions {pericentromeric alpha satellite repeats; long terminal repeats (LTR)} compared with euchromatin promoters. H4K16bio was also enriched in the repressed interleukin-2 gene promoter. The enrichment at LTR22 and promoter 1 of the sodium-dependent multivitamin transporter (SMVT) depended on biotin supply; and was significantly lower in fibroblasts …

Modulation Of Lipid Metabolism By Phytosterol Stearates And Black Raspberry Seed Oils, Mark Mckinley Ash

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Naturally occurring compounds and lifestyle modifications as combination and mono-therapy are increasingly used for dyslipidemia. Specficially, phytosterols and fatty acids have demonstrated an ability to modulate cholesterol and triglyceride metabolism in different fashions. In two separate studies, the lipid-lowering effects of black raspberry seed oil and three different phytosterol stearates were examined in order to elucidate the effects of these dietary components and the factors influencing their therapeutic actions.

The first study examined high cholesterol diets supplemented with crude and refined black raspberry seed oils, coconut oil or soybean oil. The crude and refined raspberry seed oil (RSO) treatments significantly …

Combined Therapy Of Dietary Fish Oil And Stearoyl-Coa Desaturase 1 Inhibition Prevents The Metabolic Syndrome And Atherosclerosis, J. Mark Brown, Soonkyu Chung, Janet K. Sawyer, Chiara Degirolamo, Heather M. Alger, Tam M. Nguyen, Xuewei Zhu, My-Ngan Duong, Amanda L. Brown, Caleb Lord, Ramesh Shah, Matthew A. Davis, Kathryn Kelley, Martha D. Wilson, Jennifer Madenspacher, Michael B. Fessler, John S. Parks, Lawrence L. Rudel

Department of Nutrition and Health Sciences: Faculty Publications

Background—Stearoyl-CoA desaturase 1 (SCD1) is a critical regulator of energy metabolism and inflammation. We have previously reported that inhibition of SCD1 in hyperlipidemic mice fed a saturated fatty acid (SFA)-enriched diet prevented development of the metabolic syndrome, yet surprisingly promoted severe atherosclerosis. In this study we tested whether dietary fish oil supplementation could prevent the accelerated atherosclerosis caused by SCD1 inhibition.

Methods and Results—LDLr^-/-, ApoB^100/100 mice were fed diets enriched in saturated fat or fish oil in conjunction with antisense oligonucleotide (ASO) treatment to inhibit SCD1. As previously reported, in SFA-fed mice, SCD1 inhibition dramatically protected against …

Liver Injury In Acute Fatty Liver Of Pregnancy: Possible Link To Placental Mitochondrial Dysfunction And Oxidative Stress, Sathish Kumar Natarajan, Kavitha R. Thangaraj, C. E. Eapen, Anup Ramachandran, Ashis Mukhopadhya, Mathews Mathai, Lakshmi Seshadri, Abraham Peedikayil, Banumathi Ramakrishna, Kunnissery A. Balasubramanian

Department of Nutrition and Health Sciences: Faculty Publications

Acute fatty liver of pregnancy (AFLP) is a rare disorder which is fatal if not recognized and treated early. Delivery of the feto-placental unit results in dramatic improvement in maternal liver function, suggesting a role for the placenta. However, the mechanisms by which defects in the fetus or placenta lead to maternal liver damage are not well understood and form the focus of this study. Placenta and serum were obtained at delivery from patients with AFLP, and placental mitochondria and peroxisomes were isolated. Placental mitochondrial function, oxidative stress, and fatty acid composition as well asserumantioxidants, oxidativeandnitrosative stress markers,andfatty acid analysis …

Targeted Deletion Of Hepatocyte Abca1 Leads To Very Low Density Lipoprotein Triglyceride Overproduction And Low Density Lipoprotein Hypercatabolism, Soonkyu Chung, Jenelle M. Timmins, Myngan Duong, Chiara Degirolamo, Shunxing Rong, Janet K. Sawyer, Roshni R. Singaraja, Michael R. Hayden, Nobuyo Maeda, Lawrence L. Rudel, Gregory S. Shelness, John S. Parks

Department of Nutrition and Health Sciences: Faculty Publications

Loss of ABCA1 activity in Tangier disease (TD) is associated with abnormal apoB lipoprotein (Lp) metabolism in addition to the complete absence of high density lipoprotein (HDL). We used hepatocyte-specific ABCA1 knock-out (HSKO) mice to test the hypothesis that hepatic ABCA1 plays dual roles in regulating Lp metabolism and nascent HDL formation. HSKO mice recapitulated the TD lipid phenotype with postprandial hypertriglyceridemia, markedly decreased LDL, and near absence of HDL. Triglyceride (TG) secretion was 2-fold higher in HSKO compared with wild type mice, primarily due to secretion of larger TG-enriched VLDL secondary to reduced hepatic phosphatidylinositol 3-kinase signaling. HSKO mice …

Nutrition-Related Practices And Attitudes Of Kansas Skipped-Generation(S) Caregivers And Their Grandchildren, Mary Meck Higgins, Bethany J. Murray

Department of Nutrition and Health Sciences: Faculty Publications

Despite growing numbers, the nutrition practices and attitudes of skipped-generation(s) kinship caregivers regarding feeding the dependent children in their care have not been examined. In this qualitative study, transcriptions of semi-structured interviews with 19 female and four male skipped-generation(s) Kansas caregivers (ages 47 to 80, 92% non-Hispanic whites, 83% female, 78% grandparents and 22% great-aunt or great-grandparent caregivers; caring for a range of one to four children, ages three to 18, for an average of nine years) were content analyzed for how their nutrition-related practices and attitudes had changed since parenting the first time. Sub-themes regarding practices included: being more …

Predicting Successful Aging In A Population-Based Sample Of Georgia Centenarians, Jonathan Arnold, Jianliang Dai, Lusine Nahapetyan, Ankit Arte, Mary Ann Johnson, Dorothy Hausman, Willard L. Rodgers, Robert Hensley, Peter Martin, Maurice Macdonald, Adam Davey, Ilene C. Siegler, S. Michal Jazwinski, Leonard W. Poon

Department of Nutrition and Health Sciences: Faculty Publications

Used a population-based sample (Georgia Centenarian Study, GCS), to determine proportions of centenarians reaching 100 years as (1) survivors (43%) of chronic diseases first experienced between 0–80 years of age, (2) delayers (36%) with chronic diseases first experienced between 80–98 years of age, or (3) escapers (17%) with chronic diseases only at 98 years of age or older. Diseases fall into two morbidity profiles of 11 chronic diseases; one including cardiovascular disease, cancer, anemia, and osteoporosis, and another including dementia. Centenarians at risk for cancer in their lifetime tended to be escapers (73%), while those at risk for cardiovascular disease …

Alteration Of The Gastrointestinal Microbiota Of Mice By Edible Blue-Green Algae, H. E. Rasmussen, I. Martínez, J. Y. Lee, J. Walter

Department of Nutrition and Health Sciences: Faculty Publications

Aims: To characterize the effect of edible blue-green algae (cyanobacteria) on the gastrointestinal microbiota of mice. Methods and Results: C57BL⁄6J mice were fed a diet supplemented with 0% or 5% dried Nostoc commune, Spirulina platensis, or Aphanizominon flos-aquae (w⁄w) for 4 weeks. Molecular fingerprinting of the colonic microbiota using denaturing gradient gel electrophoresis revealed that administration of N. commune induced major alterations in colonic microbiota composition, while administration of S. platensis or A. flos-aquae had a more subtle impact. Community profile analysis revealed that administration of N. commune did not reduce microbial diversity indices of the colonic microbiota. …

Hypocholesterolemic Effect Of Nostoc Commune Var. Sphaeroides Kützing, An Edible Blue-Green Alga, Heather E. Rasmussen, Kara R. Blobaum, Elliot D. Jesch, Chai Siah Ku, Young-Ki Park, Fan Lu, Timothy P. Carr, Ji-Young Li

Department of Nutrition and Health Sciences: Faculty Publications

Background Intake of an edible blue-green alga Nostoc commune var. sphaeroides Kützing (N. commune) has been shown to lower plasma total cholesterol concentration, but the mechanisms behind the hypocholesterolemic effect have not been elucidated. Aim of the study To elucidate the mechanisms underlying the cholesterol-lowering effect of N. commune in mice. Methods Male C57BL/6J mice were fed the AIN-93 M diet supplemented with 0 or 5% (wt/wt) dried N. commune for 4 weeks. Lipid levels in the plasma and liver, intestinal cholesterol absorption, and fecal sterol excretion were measured. Expression of hepatic and intestinal genes involved in cholesterol …

Effects Of Β-Alanine Supplementation And High-Intensity Interval Training On Endurance Performance And Body Composition In Men; A Double-Blind Trial, Abbie E. Smith, Ashley A. Walter, Jennifer L. Graef, Kristina L. Kendall, Jordan R. Moon, Christopher M. Lockwood, David H. Fukuda, Travis W. Beck, Joel T. Cramer, Jeffrey R. Stout

Department of Nutrition and Health Sciences: Faculty Publications

Background: Intermittent bouts of high-intensity exercise result in diminished stores of energy substrates, followed by an accumulation of metabolites, promoting chronic physiological adaptations. In addition, β-alanine has been accepted has an effective physiological hydrogen ion (H⁺) buffer. Concurrent high-intensity interval training (HIIT) and β-alanine supplementation may result in greater adaptations than HIIT alone. The purpose of the current study was to evaluate the effects of combining β-alanine supplementation with high-intensity interval training (HIIT) on endurance performance and aerobic metabolism in recreationally active college-aged men.

Methods: Forty-six men (Age: 22.2 ± 2.7 yrs; Ht: 178.1 ± 7.4 cm; Wt: …

Effects Of Β-Alanine Supplementation And High-Intensity Interval Training On Endurance Performance And Body Composition In Men; A Double-Blind Trial, Abbie E. Smith, Ashley A. Walter, Jennifer L. Graef, Kristina L. Kendall, Jordan R. Moon, Christopher M. Lockwood, David H. Fukuda, Travis W. Beck, Joel T. Cramer, Jeffrey R. Stout

Department of Nutrition and Health Sciences: Faculty Publications

Background: Intermittent bouts of high-intensity exercise result in diminished stores of energy substrates, followed by an accumulation of metabolites, promoting chronic physiological adaptations. In addition, β-alanine has been accepted has an effective physiological hydrogen ion (H⁺) buffer. Concurrent high-intensity interval training (HIIT) and β-alanine supplementation may result in greater adaptations than HIIT alone. The purpose of the current study was to evaluate the effects of combining β-alanine supplementation with high-intensity interval training (HIIT) on endurance performance and aerobic metabolism in recreationally active college-aged men.

Methods: Forty-six men (Age: 22.2 ± 2.7 yrs; Ht: 178.1 ± 7.4 cm; Wt: …

Acute Effects Of A Thermogenic Nutritional Supplement On Cycling Time To Exhaustion And Muscular Strength In College-Aged Men, Ashley A. Walter, Trent J. Herda, Eric D. Ryan, Pablo B. Costa, Katherine M. Hoge, Travis W. Beck, Jeffrey R. Stout, Joel T. Cramer

Department of Nutrition and Health Sciences: Faculty Publications

Background: The purpose of the present study was to examine the acute effects of a thermogenic nutritional supplement containing caffeine, capsaicin, bioperine, and niacin on muscular strength and endurance performance.

Methods: Twenty recreationally-active men (mean ± SD age = 21.5 ± 1.4 years; stature = 178.2 ± 6.3 cm; mass = 76.5 ± 9.9 kg; VO_{2 PEAK} = 3.05 ± 0.59 L/min^-1) volunteered to participate in this randomized, double-blinded, placebo-controlled, cross-over study. All testing took place over a three-week period, with each of the 3 laboratory visits separated by 7 days (± 2 hours). During the initial …

Initial Interaction Of Apoa-I With Abca1 Impacts In Vivo Metabolic Fate Of Nascent Hdl, Anny Mulya, Ji-Young Lee, Abraham K. Gebre, Elena Y. Boudyguina, Soonkyu Chung, Thomas L. Smith, Perry L. Colvin, Xian-Cheng Jiang, John S. Parks

Department of Nutrition and Health Sciences: Faculty Publications

We investigated the in vivo metabolic fate of preb HDL particles in human apolipoprotein A-I transgenic (hA-I Tg) mice. Pre-b HDL tracers were assembled by incubation of [125I]tyramine cellobiose-labeled apolipoprotein A-I (apoA-I) with HEK293 cells expressing ABCA1. Radiolabeled pre-b HDLs of increasing size (pre-b1, -2, -3, and -4 HDLs) were isolated by fast-protein liquid chromatography and injected into hA-I Tg-recipient mice, after which plasma decay, in vivo remodeling, and tissue uptake were monitored. Pre-b2, -3, and -4 had similar plasma die-away rates, whereas pre-b1 HDL was removed 7-fold more rapidly. Radiolabel recovered in liver and kidney 24 h after tracer …

Lipid Extract Of Nostoc Commune Var. Sphaeroides Kützing, A Blue-Green Alga, Inhibits The Activation Of Sterol Regulatory Element Binding Proteins In Hepg2 Cells, Heather E. Rasmussen, Kara R. Blobaum, Young-Ki Park, Sarah J. Ehlers, Fan Lu, Ji-Young Lee

Department of Nutrition and Health Sciences: Faculty Publications

Nostoc commune var. sphaeroides Kützing (N. commune), a blue-green alga, has been used as both a food ingredient and in medicine for centuries. To determine the effect of N. commune on cholesterol metabolism, N. commune lipid extract was incubated at increasing concentrations (25–100 mg/L) with HepG2 cells, a human hepatoma cell line. The addition of N. commune lipid extract markedly reduced mRNA abundance of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) and LDL receptor (LDLR) (P < 0.05), with a concomitant decrease in their protein expression (P < 0.001). Reduced HMGR activity by 90% with N. commune lipid extract confirmed the inhibitory role of N. commune in cholesterol synthesis (P < 0.006). To elucidate a molecular mechanism underlying the repression of HMGR and LDLR by N. commune lipid extract, expression of sterol regulatory …

Effects Of A Supplement Designed To Increase Atp Levels On Muscle Strength, Power Output, And Endurance, Trent J. Herda, Eric D. Ryan, Jeffrey R. Stout, Joel T. Cramer

Department of Nutrition and Health Sciences: Faculty Publications

Background: The present study examined the acute effects of a nutritional supplement intended to improve adenosine triphosphate (ATP) concentrations on vertical jump height, isometric strength of the leg extensors, leg extension endurance, and forearm flexion endurance.

Methods: Twenty-four healthy men (mean age ± SD = 23 ± 4 yrs, stature = 181 ± 7 cm, and body mass = 82 ± 12 kg) volunteered to complete a familiarization trial plus 2 randomly-ordered experimental trials separated by a 7-day washout period. Participants received either 6 (body mass < 91 kg) or 8 (body mass ≥ 91 kg) tablets of the treatment (TR; 625 mg of adenylpyrophosphoric acid and calcium pyruvate, 350.8 mg of cordyceps sinensis extract and yohimbine hydrochloride) or placebo (PL; 980 mg of microcrystalline cellulose) 1 hour prior to the following tests: countermovement vertical jump (CVJ), forearm flexion repetitions to exhaustion, isometric maximal voluntary contractions (MVCs) of the leg extensors, and a 50-repetition maximal concentric isokinetic leg extension endurance test.

Results: There were no differences between the TR and PL trials for CVJ height …

The Effect Of Beta-Alanine Supplementation On Neuromuscular Fatigue In Elderly (55–92 Years): A Double-Blind Randomized Study, Jeffrey R. Stout, B. Sue Graves, Abbie E. Smith, Michael J. Hartman, Joel T. Cramer, Travis W. Beck, Roger C. Harris

Department of Nutrition and Health Sciences: Faculty Publications

Background: Ageing is associated with a significant reduction in skeletal muscle carnosine which has been linked with a reduction in the buffering capacity of muscle and in theory, may increase the rate of fatigue during exercise. Supplementing beta-alanine has been shown to significantly increase skeletal muscle carnosine. The purpose of this study, therefore, was to examine the effects of ninety days of beta-alanine supplementation on the physical working capacity at the fatigue threshold (PWC_FT) in elderly men and women.

Methods: Using a double-blind placebo controlled design, twenty-six men (n = 9) and women (n = 17) (age ± …

Inhibition Of Stearoyl-Coa Desaturase 1 (Scd1) Dissociates Insulin Resistance And Obesity From Atherosclerosis, J. Mark Brown, Soonkyu Chung, Janet K. Sawyer, Chiara Degirolamo, Heather M. Alger, Tam Nguyen, Xuewei Zhu, My-Ngan Duong, Amanda L. Wibley, Ramesh Shah, Matthew A. Davis, Kathryn Kelley, Martha D. Wilson, Carol Kent, John S. Parks, Lawrence L. Rudel

Department of Nutrition and Health Sciences: Faculty Publications

Background—Stearoyl-CoA Desaturase 1 (SCD1) is a well-known enhancer of the metabolic syndrome. The purpose of the present study was to investigate the role of SCD1 in lipoprotein metabolism and atherosclerosis progression.

Methods and Results—Antisense oligonucleotides were used to inhibit SCD1 in a mouse model of hyperlipidemia and atherosclerosis (LDLr^-/-Apob^100/100). In agreement with previous reports, inhibition of SCD1 protected against diet-induced obesity, insulin resistance, and hepatic steatosis. However, unexpectedly SCD1 inhibition strongly promoted aortic atherosclerosis, which could not be reversed by dietary oleate. Further analyses revealed that SCD1 inhibition promoted accumulation of saturated fatty acids …

Total Body Water Estimations In Healthy Men And Women Using Bioimpedance Spectroscopy: A Deuterium Oxide Comparison, Jordan R. Moon, Sarah E. Tobkin, Michael D. Roberts, Vincent J. Dalbo, Chad M. Kerksick, Michael G. Bemben, Joel T. Cramer, Jeffrey R. Stout

Department of Nutrition and Health Sciences: Faculty Publications

Background: Total body water (TBW) estimations have been used to estimate body composition, particularly fat-free mass, to aid in nutritional interventions, and to monitor hydration status. In the past, bioimpedance spectroscopy (BIS) devices have been used to estimate TBW. Previous investigations have examined the validity of the XiTRON 4000B (XiTRON Technologies) BIS device for estimating TBW. Recently, a new BIS device (Imp™ SFB7) has become available, claiming greater precision when estimating TBW. The Imp™ SFB7 (SFB7) is based on similar BIS principles, while offering increased portability and a greater range of frequencies when compared to older devices, such as the …

Minimal Nutrition Intervention With High-Protein/Low-Carbohydrate And Low-Fat, Nutrient-Dense Food Supplement Improves Body Composition And Exercise Benefits In Overweight Adults: A Randomized Controlled Trial, Christopher M. Lockwood, Jordan R. Moon, Sarah E. Tobkin, Ashley A. Walter, Abbie E. Smith, Vincent J. Dalbo, Joel T. Cramer, Jeffrey R. Stout

Department of Nutrition and Health Sciences: Faculty Publications

Background: Exercise and high-protein/reduced-carbohydrate and -fat diets have each been shown separately, or in combination with an energy-restricted diet to improve body composition and health in sedentary, overweight (BMI > 25) adults. The current study, instead, examined the physiological response to 10 weeks of combined aerobic and resistance exercise (EX) versus exercise + minimal nutrition intervention designed to alter the macronutrient profile, in the absence of energy restriction, using a commercially available high-protein/low-carbohydrate and low-fat, nutrient-dense food supplement (EXFS); versus control (CON).

Methods: Thirty-eight previously sedentary, overweight subjects (female = 19; male = 19) were randomly assigned to either CON (n …