Molecular, Genetic, and Biochemical Nutrition Commons^™

Total Body Water Estimations In Healthy Men And Women Using Bioimpedance Spectroscopy: A Deuterium Oxide Comparison, Jordan R. Moon, Sarah E. Tobkin, Michael D. Roberts, Vincent J. Dalbo, Chad M. Kerksick, Michael G. Bemben, Joel T. Cramer, Jeffrey R. Stout

Department of Nutrition and Health Sciences: Faculty Publications

Background: Total body water (TBW) estimations have been used to estimate body composition, particularly fat-free mass, to aid in nutritional interventions, and to monitor hydration status. In the past, bioimpedance spectroscopy (BIS) devices have been used to estimate TBW. Previous investigations have examined the validity of the XiTRON 4000B (XiTRON Technologies) BIS device for estimating TBW. Recently, a new BIS device (Imp™ SFB7) has become available, claiming greater precision when estimating TBW. The Imp™ SFB7 (SFB7) is based on similar BIS principles, while offering increased portability and a greater range of frequencies when compared to older devices, such as the …

Effects Of A Supplement Designed To Increase Atp Levels On Muscle Strength, Power Output, And Endurance, Trent J. Herda, Eric D. Ryan, Jeffrey R. Stout, Joel T. Cramer

Department of Nutrition and Health Sciences: Faculty Publications

Background: The present study examined the acute effects of a nutritional supplement intended to improve adenosine triphosphate (ATP) concentrations on vertical jump height, isometric strength of the leg extensors, leg extension endurance, and forearm flexion endurance.

Methods: Twenty-four healthy men (mean age ± SD = 23 ± 4 yrs, stature = 181 ± 7 cm, and body mass = 82 ± 12 kg) volunteered to complete a familiarization trial plus 2 randomly-ordered experimental trials separated by a 7-day washout period. Participants received either 6 (body mass < 91 kg) or 8 (body mass ≥ 91 kg) tablets of the treatment (TR; 625 mg of adenylpyrophosphoric acid and calcium pyruvate, 350.8 mg of cordyceps sinensis extract and yohimbine hydrochloride) or placebo (PL; 980 mg of microcrystalline cellulose) 1 hour prior to the following tests: countermovement vertical jump (CVJ), forearm flexion repetitions to exhaustion, isometric maximal voluntary contractions (MVCs) of the leg extensors, and a 50-repetition maximal concentric isokinetic leg extension endurance test.

Results: There were no differences between the TR and PL trials for CVJ height …

The Effect Of Beta-Alanine Supplementation On Neuromuscular Fatigue In Elderly (55–92 Years): A Double-Blind Randomized Study, Jeffrey R. Stout, B. Sue Graves, Abbie E. Smith, Michael J. Hartman, Joel T. Cramer, Travis W. Beck, Roger C. Harris

Department of Nutrition and Health Sciences: Faculty Publications

Background: Ageing is associated with a significant reduction in skeletal muscle carnosine which has been linked with a reduction in the buffering capacity of muscle and in theory, may increase the rate of fatigue during exercise. Supplementing beta-alanine has been shown to significantly increase skeletal muscle carnosine. The purpose of this study, therefore, was to examine the effects of ninety days of beta-alanine supplementation on the physical working capacity at the fatigue threshold (PWC_FT) in elderly men and women.

Methods: Using a double-blind placebo controlled design, twenty-six men (n = 9) and women (n = 17) (age ± …

Cholesterol-Lowering Properties Of Plant Sterols Esterified With Beef Tallow Fatty Acids In Hamsters, David M. Guderian Jr., Heather E. Rasmussen, Curtis A. Wray, Patrick Dussault, Timothy P. Carr

Department of Nutrition and Health Sciences: Faculty Publications

Plant sterol esters (PSE) reduce plasma low-density lipoprotein cholesterol concentration by inhibiting cholesterol absorption. Previous work in our laboratory indicated that PSE containing stearic acid (SA), compared to linoleic acid, were significantly more effective at reducing cholesterol absorption. We tested the cholesterol lowering properties of PSE containing fatty acids from beef tallow, a natural and abundant source of SA. Male hamsters were fed diets containing 0.0%, 0.5%, 1.0%, or 5.0% beef tallow PSE for 4 weeks. Dose-dependent reductions (P < .05) were observed in cholesterol absorption, liver esterified cholesterol, and plasma nonhigh-density lipoprotein cholesterol concentration; hamsters fed 5.0% PSE exhibited reductions of 56%, 98%, and 38%, respectively, compared to controls. Concurrent increases (P < .05) in fecal sterol excretion were also observed. In a second hamster study, the cholesterol-lowering properties of beef tallow PSE were compared to hamsters fed molar equivalents of free plant sterols (PS) and free SA. Beef tallow PSE were significantly more effective at reducing liver and plasma cholesterol concentrations than PS and SA consumed individually. Whether PS and SA act through independent or synergistic mechanisms will require further research, although the present findings support the use of dietary PSE containing beef tallow fatty acids in the management of hypercholesterolemia.

Feeding Drosophila A Biotin-Deficient Diet For Multiple Generations Increases Stress Resistance And Lifespan And Alters Gene Expression And Histone Biotinylation Patterns, Erin M. Smith, Jia Tse Hoi, Joel C. Eissenberg, James D. Shoemaker, Wendi S. Neckameyer, Anne M. Ilvarsonn, Lawrence G. Harshman, Vicki L. Schlegel, Janos Zempleni

Department of Nutrition and Health Sciences: Faculty Publications

Caloric restriction increases stress resistance and lifespan in Drosophila melanogaster and other species. The roles of individual nutrients in stress resistance and longevity are largely unknown. The vitamin biotin is a potential candidate for mediating these effects, given its known roles in stress signaling and gene regulation by epigenetic mechanisms, i.e., biotinylation of histones. Here, we tested the hypothesis that prolonged culture of Drosophila on biotin-deficient medium increases stress resistance and lifespan. Flies were fed a biotin-deficient diet for multiple generations; controls were fed a biotin-normal diet. In some experiments, a third group of flies was fed a biotin-deficient diet …

Aromatic Compounds In Three Varieties Of Turnip Greens Harvested At Three Maturity Levels, Georgia Jones, Ola Goode Sanders, Casey Grimm

Department of Nutrition and Health Sciences: Faculty Publications

Turnip greens (Brassica rapa) are commonly consumed in the southern U.S.A. Typically, they have a bitter taste, which increases with maturity, probably because of increased levels of glucosinolates. While glucosinolate degradation products have been isolated from various members of the Brassica family, the effect of variety and maturity on these products has not been determined. This study focused on the glucosinolate degradation products of three varieties of turnip greens: Purple Top, Seven Top and Tokyo Cross, harvested 45, 60 and 75 days after planting. Four volatile components (benzene acetonitrile, benzene propane nitrile, 1H-indole-3-acetonitrile and benzene ethyl isothiocyanate) were isolated, identified …

Reduction In Cholesterol Absorption Is Enhanced By Stearate-Enriched Plant Sterol Esters In Hamsters, Heather E. Rasmussen, David M. Guderian Jr., Curtis A. Wray, Patrick H. Dussault, Vicki L. Schlegel, Timothy P. Carr

Department of Nutrition and Health Sciences: Faculty Publications

Consumption of plant sterol esters reduces plasma LDL cholesterol concentration by inhibiting intestinal cholesterol absorption. Commercially available plant sterol esters are prepared by esterifying free sterols to fatty acids from edible plant oils such as canola, soybean, and sunflower. To determine the influence of the fatty acid moiety on cholesterol metabolism, plant sterol esters were made with fatty acids from soybean oil (SO), beef tallow (BT), or purified stearic acid (SA) and fed to male hamsters for 4 wk. A control group fed no plant sterol esters was also included. Hamsters fed BT and SA had significantly lower cholesterol absorption …

Renal Damage In Experimentally-Induced Cirrhosis In Rats: Role Of Oxygen Free Radicals, Sathish Kumar Natarajan, Jayasree Basivireddy, Anup Ramachandran, Simmy Thomas, Prabhu Ramamoorthy, Anna B. Pulimood, Molly Jacob, Kunissery A. Balasubramanian

Department of Nutrition and Health Sciences: Faculty Publications

Cirrhosis with ascites is associated with impaired renal function accompanied by sodium and water retention. Although it has been suggested that mediators such as nitric oxide play a role in the development of renal failure in this situation, other mechanisms underlying the process are not well understood. This study examined the role of oxidative stress in mediating renal damage during the development of cirrhosis in order to understand mechanisms involved in the process. It was shown that carbon tetrachloride– or thioacetamide-induced cirrhosis in rats results in oxidative stress in the kidney as seen by increased lipid peroxidation and protein oxidation, …

Intestinal Mucosal Alterations In Rats With Carbon Tetrachloride-Induced Cirrhosis: Changes In Glycosylation And Luminal Bacteria, Sathish Kumar Natarajan, Prabhu Ramamoorthy, Simmy Thomas, Jayasree Basivireddy, Gagandeep Kang, Anup Ramachandran, Anna B. Pulimood, K. A. Balasubramanian

Department of Nutrition and Health Sciences: Faculty Publications

Spontaneous bacterial peritonitis is a major cause of mortality after liver cirrhosis. Altered permeability of the mucosa and deficiencies in host immune defenses through bacterial translocation from the intestine due to intestinal bacterial overgrowth have been implicated in the development of this complication. Molecular mechanisms underlying the process are not well known. In order to understand mechanisms involved in translocation of bacteria, this study explored the role of oxidative stress in mediating changes in intestinal mucosal glycosylation and luminal bacterial content during cirrhosis. CCl₄-induced cirrhosis in rats led to prolonged oxidative stress in the intestine, accompanied by increased …

Mechanomyographic Amplitude And Frequency Responses During Dynamic Muscle Actions: A Comprehensive Review, Travis W. Beck, Terry J. Housh, Joel T. Cramer, Joseph P. Weir, Glen O. Johnson, Jared W. Coburn, Moh H. Malek, Michelle Mielke

Department of Nutrition and Health Sciences: Faculty Publications

The purpose of this review is to examine the literature that has investigated mechanomyographic (MMG) amplitude and frequency responses during dynamic muscle actions. To date, the majority of MMG research has focused on isometric muscle actions. Recent studies, however, have examined the MMG time and/or frequency domain responses during various types of dynamic activities, including dynamic constant external resistance (DCER) and isokinetic muscle actions, as well as cycle ergometry. Despite the potential influences of factors such as changes in muscle length and the thickness of the tissue between the muscle and the MMG sensor, there is convincing evidence that during …

Orientation To Nutrition Care Process Standards Improves Nutrition Care Documentation By Nutrition Practitioners, Nancy Hakel-Smith, Nancy M. Lewis, Kent M. Eskridge

Department of Nutrition and Health Sciences: Faculty Publications

Objective — To compare documentation of two groups of clinical nutrition practitioners for evidence of the nutrition care process.

Design — This study used a comparative descriptive design. A retrospective chart review was conducted on all nutrition documentation in closed patient records. Documentation of two groups of nutrition practitioners (institution A = practitioners who received initial orientation and routine reinforcement in use of nutrition care process standards; institution B = practitioners who received orientation in use of a further assessment and medical nutrition therapy intervention procedure) was compared for evidence of a six-step nutrition care process.

Sample/Setting — The sample …

Serum Lipids Of Physically Active Adults Consuming Omega-3 Fatty Acid–Enriched Eggs Or Conventional Eggs, Carrie A. Sindelar, Sarah B. Scheerger, Sheri L. Plugge, Kent M. Eskridge, Rosemary C. Wander, Nancy M. Lewis

Department of Nutrition and Health Sciences: Faculty Publications

This study was designed to compare the effects of the consumption of one omega-3 (n-3) polyunsaturated fatty acid (PUFA)–enriched egg or one conventional egg on serum lipids in physically active adults. A total of 12 adults (mean age 33 ± 7 years, mean body mass index [BMI] 24 ± 3) were recruited, and dietary treatments were randomly assigned. After a 2-week lead-in period (baseline), participants received each 4-week treatment in a crossover arrangement with a 4-week washout period between treatments. Participants completed a 3-day food record at baseline and during each treatment period. Food records were analyzed for carbohydrates, protein, …