Nutrition Commons^™

The Effect Of Heat Treatment Of Fresh Frozen Vells On Rennin Extractability, Leesther Mifflin Holm

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A procedure was developed for the extraction of rennet from fresh frozen vells. Frozen vells were minced in a Model VCM-25 Hobart Vertical Cutter/Mixer. Dry sodium chloride was added until the salt concentration in the moisture of the tissue was 10%. Salted tissue was placed in galvanized steel cells and heated in a water bath to 49, 54, 57 and 60 C for 0, 15, 30, 45 and 60 minutes. The heated tissue was dried to about 4% moisture at ambient temperature with the aid of an electric fan. The dried salted tissue was extracted with a sodium chloride solution …

Purification And Characterization Of A Protease From A Lamb Gastric Extract Used For Cheese Flavor Improvement, Ramjibhai V. Chaudhari

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

An assay for catheptic activity of lamb gastric tissue extract has been proposed which involves the use of a pH 3.5 hemoglobin substrate following activation of zymogens at pH 2.0, 25C for 30 min.; and inactivation at pH 8.0, 40C for 30 min thereby eliminating the effects of pepsin and rennin. Cathepsin was isolated and purified by ammonium sulfate fractionation, acetone precipitation and gel filtration. The purified cathepsin represented approximately 50 fold increase in specific activity over the original extract and a recovery of 15% of the original activity. Degree of purity was monitored by isoelectric focusing in polyacrylamide gels. …

Detection And Quantitation Of Staphylococcus Aureus Deoxyribonuclease In Cheese, Cyril Newell Maughan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A specific method has been developed for the extraction and measurement of staphylococcal nuclease in cheese in which Staphylococcus aureus has grown. Ten grams of cheese sample were homogenized with ninety milliliters of pH ten buffer for three minutes. Ammonium sulfate fractionation was used and a forty to eighty percent fraction was collected and concentrated using ultrafilters. The nuclease activity was determined using a toluidine blue deoxyribonucleic acid agar slide method and a spectophotometric method. The DNA agar slide method was used to compare staphylococcal growth with nuclease production in cheese under varying conditions. When Staphylococcus aureus plate counts indicated …

Assay And Control Of Staphylococcal Enterotoxin A Development In Cheddar Cheese Slurries, Niranjan R. Gandhi

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Attempts were made to adapt the microtiter hemagglutination inhibition assay technique for the assay of enterotoxin A. The presence of a potent hemagglutinin in crude and partially purified preparations and the instability of sensitized erythrocytes prevented its use for routine analysis of enterotoxin from culture media and foods.

A capillary tube immunological assay was developed in which 1 μ g of enterotoxin/ml was detected in less than 1 hr . Interfacial reaction of antisera and enterotoxin solutions in a 1 mm internal diameter capillary tube allowed rapid detection and serological typing of enterotoxins.

Staphylococcus aureus growth and enterotoxin A development …

Essential Tyrosine Residues In Calf Liver Uridine Diphosphoglucose Pyrophosphorylase, E.C. 2.7.7.9., Robert Carl Bachmann

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The catalytic necessity of tyrosine residues in uridine diphospho- glucose pyrophosphorylase [E.C. 2.7.7.9] was investigated. Chemical modification of the pyrophosphorylase by N-acetylimidazole indicated that tyrosine residues were essential for activity. Approximately 23 of 112 tyrosines per molecule of 475,000 Daltons could be 0-acetylated. Solvent perturbation difference spectroscopy supported this number of exposed tyrosine side chains and in conjunction with chemical modification indicated that at least 11 to 12 tyrosyl residues per protein molecule are fully exposed. it her subst rate, uridine t riphosphate or uridine diphosphoglucose, afforded significant protection against inactivation by N-acetylimidazole.

The significance of these tyrosine residues is …

Continuous Flow Analysis Of Non-Casein Protein In Milk, Robert Steven White

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Technicon AutoAnalyzer II was evaluated for automatic quantitation of non-casein protein (NCP) in milk. The Lowry method for colorimetric measurement of proteins was adapted to the automated method and found to be accurate in the determination of non-casein protein in milk. The automated Lowry method obeyed Beer's law. Casein content was calculated by difference between total protein determined by infrared milk analysis (IRMA) and non-casein protein. In the analysis of twenty-five replicate milk samples, the standard deviation was 0.32 and the coefficient of variability was 0.90. The casein content as a percentage of total protein in the milk of …

Formation And Control Of Chlorophyll And Solanine In Tubers Of Solanum Tuberosum, L. And Evaluation Of Solanine Toxicity, Bhaskarrao C. Patil

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Chlorophyll and solanine syntheses as influenced by cultivars, specific gravities, light intensities, and duration of exposure to light were studied in potato (Solanum tuberosum, L.) tubers. Chemicals, gamma radiation, clear polyethylene packaging in 15 per cent CO₂, and storage at subatmospheric pressure were employed to control the formation of these two compounds. The toxicity of solanine to mice, the fungus Trichoderma viride, and cholinesterase inhibition patterns in rabbits and a dog were evaluated. The influence of the central nervous system stimulating drugs was investigated to find if they counteracted solanine toxicity.

Of the 11 cultivars …

Study Of Air Cell Migration And The Effect Of Whipping Temperature On The Overrun, Body And Storage Stability Of A Dairy-Based Frozen Whipped Topping, William J. Locker

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A dairy-based whipped topping consisting of 22.0 percent milk fat, 7.5 percent milk solids-not-fat, 12.0 percent sucrose, 10.0 percent corn syrup solids, 0.60 percent gum arabic, 0.06 percent carrageenin, 0.19 percent polyoxyethylene sorbitan rnonostearate, 0.19 percent polyoxythylene sorbitan tristearate, and 0.12 percent sodium stearoyl-2-lactylate was developed that would withstand the rigors of frozen storage. The best products were obtained when the topping was whipped on a Creamery Package 3M-30 continuous type ice cream freezer. Toppings whipped in the laboratory at temperatures higher or lower than -2.2 to -1.0 centigrade were weak and slightly wet. After 18 days frozen storage the …

Serum Lipids And Urinary Estrogens Of Non-Pregnant Menstruating Young Women, Shiao-Fan Lee

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Twelve university women students served as experimental subjects in a study of the serum lipids and urinary estrogens of healthy nonpregnant menstruating young women, who were living under their usual conditions.

The subjects maintained constant weight on their ordinary diets during the entire study period. Antecubital blood and 24-hour urine specimens were collected on certain days which represented different stages of the menstrual cycle. Quantitative analyses were made on serum total cholesterol, lipid phosphorus (phospholipids), triglycerides and total lipids. Gas-liquid chromatographic analysis of the fatty acid composition of each serum lipid component was also made. Urinary estrone, 17β- estradiol and …

Effects Of Controlled Atmosphere Storage On Specific Biochemical Changes In Apricot And Peach Fruits, Bartley Norman Wankier

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The effects of controlled atmosphere storage vs conventional refrigerated storage on the metabolism of Moorpark and Large Early Montgamet apricot and Elberta peach fruits were studied. The controlled atmospheres studied were 0.0 percent CO₂-5.0 percent O₂; 2.5 percent CO₂-5.0 percent O₂; 5.0 percent CO₂-2.5 percent O₂; 7.5 percent CO₂-5.0 percent O₂; as compared to conventional refrigerated storage. Nitrogen percent was assumed to be the difference between the total CO₂ and O₂ content minus 100.

Fruits were periodically removed from the storage and …

The Effects Of Sources And Levels Of Vitamin A And D On Some Aspects Of Sulfur Metabolism, Akbar Malekpour

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Two trials were conducted using 48 infant pigs of both sexes. Pigs were taken from the sow when three to seven days old and placed in wire-bottomed metal cages by pairs. Animals in the first trial received synthetic diets containing 100 or 1000 international units of ergocalciferol and 1000 or 4000 international units of retinyl acetate or four or sixteen milligrams of beta carotene per kilogram of feed. Baby pigs in the second trial received the same synthetic diet plus 1000 international units of ergocalciferol (D₂) or cholecalciferol (D₃) and 2000 international units of vitamin A …

Effects Of Water Hardness On Processed Quality Of Carrots, Sweet Cherries, And Apricots, Jack C. Chiang

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Honey Sweet carrots were canned with Ethyienediamine tetracetic acid (CaNa₂EDTA) and Sodium hexametaphosphate (Ha-HMP) at five different water hardness (0 , 20, 40, 80, 160 ppm of calcium and 20 of magnesium) , then stored at temperatures of 70 and 100 F. Evaluations were made at sixty-day i intervals for six months. Firmness and color degradation decreased significantly when water hardness or storage time increased. Under storage at 100 F and 0 hardness of water, the decrease of color and firmness was constantly accelerated.

When hard water (above 80 ppm or below 40 ppm) was used for …

Nutritive Values Of Russian Wildrye, Crested Wheatgrass, And Intermediate Wheatgrass Grazed By Cattle On Utah Foothill Ranges, George W. Mitchell

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A comparative index to digestible nutrients was established for Russian wildrye, crested wheatgrass, and intermediate wheatgrass seeded on foothill areas of Utah. The nutritive values of these grasses were compared by the lignin ratio method, the chlorophyllan ratio method, and the in vivo rumen bag process.

Average digestion coefficients computed by the lignin ratio method for dry matter, ether extract, cellulose, and gross energy were highest for intermediate wheatgrass. Protein was more digestible for Russian wildrye. Digestible energy was adequate in all species to meet requirements for late lactation.

Results from the chlorophyllan ratio method were excessively high and were …

Quantitative Evaluation Of Saponin Content In Du Puits Alfalfa Foliage (Medicago Sativa L.) With Trichoderma, Wang Li-Chun

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Selections in Du Puits alfalfa (Medicago sativa L. ) were evaluated for saponin content. The data obtained from the Trichoderma bioassay were converted to saponin values by regressions and standard curves. It was necessary to adjust the concentration of alfalfa extract in the bioassay so that the saponin content in alfalfa was estimated in that portion of the curve showing a marked response to saponin. A different regression was established for each group of low and high saponin plants of Du Puits alfalfa. The standard curve prepared with extract of Du Puits alfalfa used on the basis of its …

Food And Nutritional Needs Of The World Population By 2000 A. D., Sitaram Shankar Kharbas

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Investigations were carried out to project the food needs of the world population by 2000 A. D., and to explore the possibilities of meeting these requirements.

About 70 percent of the world population presently living in developing countries do not get adequate diets, and suffer from many diseases of under-nutrition and malnutrition. They are unable to increase food production themselves. People in the developed nations have enough food and their food production is increasing at a rate faster than the rate of growth of population.

Cultivable land is the main source of food for man. The seas can also be …

Physio-Chemical And Toxicological Studies On Controlled Atmosphere Storage Of Certain Deciduous Fruits, Neil Adair Littlefield

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The effects of controlled atmosphere on the metabolism of Golden Delicious and Rome Beauty apples, Anjou pears, and Lambert and Bing cherries were studied with respect to changes caused in the normal metabolic functions carried on during storage of these fruits. Atmospheres consisting of 1.5 per cent CO₂ and 2.5 per cent O₂ for the apples and pears and 10.5 per cent CO₂ and 2.5 per cent O₂ for the cherries were employed.

Fungi of the strains Botrytis alli, Rhizopus nigricans, and Penicillium expansum were subjected to controlled atmospheres at 21° C to determine …

Development And Evaluation Of A Waiver Examination For The Introductory Foods Course At Utah State University, Virginia Clark Bragg

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A written objective-type waiver examination for the introductory foods course at Utah State University has been developed and evaluated.

Results of the study showed that the examination improved with revision. In addition, it was found that there was a statistically significant relationship between the score students achieved on the waiver examination and the total numerical score representing the final grade in the basic foods course.

There was apparently no significant relationship between the number of years experience students had in food preparation, the type of experience in food preparation, and their performance on the waiver examination. Apparently depth of knowledge …

The Effect Of Oven Temperature, Cooking Method, And Breed On The Tenderness, Juiciness, And Flavor Of Beef, Carma B. Stembridge

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The effect of oven temperature, dry-roasting, and breed on the eating quality of beef was determined by sensory and objective methods. Two adjacent standing rib and two adjacent chuck roasts of prime, choice, and good grades from animals of Hereford, Shorthorn, and Charolais breeding were dry-roasted. Control roasts (9-12th ribs) were cooked at 325 F. Experimental roasts (6-8th rib and two chucks) were assigned an oven temperature of 325 or 250 F at random. All roasts were allowed to reach ah internal temperature of 155 F as recorded on a potentiometer.

Tenderness, as determined by a Warner-Bratzler shear, was greater …

5 Β- Pregnane- 3Α- 20Α- Diol Excretion In Urine Of University Women During The Menstrual Period, Rita Shin-Hui Liao

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Five normal healthy young university women while on self-chosen diets and living under normal conditions served as experimental subjects in this study. Urinary pregnanediol was measured for 10 days of the menstrual cycle. Meal frequency (3 meals vs. 2 meals with no breakfast) was also included in the study.

A modification of the method of Eberlein and Bongiovanni on thin layer chromatography was used to determine pregnanediol in the urine sample.

Urine pregnanediol excretion was in the pattern as investigated by other workers. The values rose in the latter half of the menstrual cycle, and fell prior to the onset …

Determination Of Estrone, 17b-Estradiol And Estriol In Urine Of Young Adults, Shiao-Fan Lee

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A study was made of the urinary excretion of estrogens of healthy university students consuming self-selected diets living under home conditions (4 men and 5 women). Two 31-day test periods were used. During the two test periods, two meal patterns were used; three meals a day or two meals a day with no lunch. Weight was kept constant. Calories, protein, carbohydrate, and fat of their diets were calculated.

Urine specimens were collected twice a week, and additional samples were also collected on specified days for female subjects. Urinary estrogens were determined by using Brown's method (1955, 1957) with some modifications. …

Lipid Composition Of Human Red Blood Cells And White Blood Cell-Platelets With And Without Administration Of Nicotinic Acid, Nuggehalli Narayan Tara

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Five normal, healthy, men (age 33-42) served as experimental subjects. The subjects were on self-chosen diets and received a test-dose of buffered nicotinic acid (Nico-Span in capsule form) of 2 gm daily for three weeks, from the second through the fourth weeks, of the six and one-half week test period. About 30 ml of venous blood was obtained twice weekly from each subject, after an overnight fast of at least 12 hours. Each subject collected a 24-hour urine sample of the day prior to the blood sampling and also kept a record of food eaten.

The blood was separated into …

Serum Lipid Components Of University Women During The Menstrual Cycle, Hih-Min Sang

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Four healthy university women students (age 21-26) while on self-selected diets and living under normal living conditions served as experimental subjects in a study of changes of serum total lipids, distribution of lipid classes, and the fatty acid composition of lipid fractions during the menstrual cycle. Effect of meal frequency (2 meals vs 3 meals with no breakfast) was also included.

Total lipids were extracted from serum. Chemical determinations of total lipids, total and free cholesterols, and lipid phosphorous were made on these extracts. Concentration of cholesterol esters and phospholipids were calculated and triglycerides plus free fatty acids were calculated …

Venison Flavor: The Free Fatty Acid Content Of Fat From Lean Meat Tissue, Von T. Mendenhall

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Millions of pounds of venison are harvested each year in the United States. Over 12,000,000 pounds of dressed venison are harvested during the regular season each year in the State of Utah. The hunters of venison contribute approximately 10 1/2 billion dollars to the economy of the state for licenses, lodging, transportation, and other expense of hunting. In many cases this source of meat is a significant part of the household meat supply. Venison is often wasted, however, because of its undesirable flavor. The lack of knowledge as to the causes of variation in venison flavor has prompted this study. …

Serum Alpha-Tocopherol, Cholesterol, And Total Lipid Values And Unsaturation Of Lipids In 33- To 60-Year Old Adults On Self-Selected Diets At Different Levels Of Polyunsaturated Fat, Marjorie Miner Christiansen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The levels and weekly variations of certain lipid components were investigated in healthy adults, 33 to 60 years of age, on self-selected diets under home-living conditions with and without an increased proportion of polyunsaturated fats for a period of 26 weeks. Ten individuals served as controls and 16 were assigned to the experimental diet. Each of the groups contained an equal number of men and women. The experimental diet provided a ratio of polyunsaturated to saturated fatty acids of approximately one, with the substitution of vegetable oils for animal fats within the normal eating patterns of the participants. The control …

Amino Acids As Precursors Of Volatile Components In Tomato Fruit, Ming-Ho Yu

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The composition of tomato fruit at nine different maturity stages was studied. The dry matter content decreased gradually, whereas the alcohol soluble material and non-protein nitrogen increased with ripening. Total nitrogen decreased up to the large green stage and then increased during ripening. Starch accumulated until nearly the large green stage and then diminished rapidly. Of the two components of starch, amylose decreased much faster than amylopectin, and the ratios of amylopectin and amylose thus increased during ripening. Among the amino acids, glutamic acid was present in an exceptionally high concentration, and its concentration doubled as the fruit ripened. A …

A Two Year Course Outline In Quantity Foods For Intermountain High School Girls, June C. Larson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Bureau of Indian Affairs has recently completed or is now in the process of building 19 new grade schools on the Navajo reservation. Some of these schools are boarding schools, and some are day schools, but each new school needs school lunch personnel. To meet this need intermountain School added to their curriculum a course in Quantity Foods to be given to Junior and Senior High School girls as a two year vocation. This necessitated the development of a 2 year course outline for the 2 year Quantity Food class, which gave the girls 4 hours daily practical training …

A Longitudinal Study: The Relationship Of Various Physical Characteristics To Serum Cholesterol In Preadolescents And Adolescents, Carol Ann Milligan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Although many theories have been formulated concerning atherosclerosis and its relationship to the incidence of coronary heart disease, few actual facts are known. It is known that atherosclerosis is very complex and appears to be influenced by many factors. It is postulated that an elevated serum cholesterol level is one predisposing factor.

Serum cholesterol levels are likewise influenced by many factors. Age, sex, body build, exercise, socio-economic levels, smoking, health status, weight loss or gain, dietary fat, endocrine function, and stress have been shown to be related to serum cholesterol levels, and according to some authors also are related to …

A Comparative Gas Chromatographic Study Of Venison And Beef Flavors, K. Prakash Reddy

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Venison is an important food item in the diet of the people of the United States. Deer is hunted both as sport and for food. Annually, nearly 180,000 hunters go afield in Utah in search of deer. Over 130,000 animals are killed which amounts to an annual harvest of over 15,000,000 pounds of dressed venison. This contributes about 10 1/2 million dollars to the economy of the state from licenses, lodgings, transportation, and other expenses of hunting.

Studies On The Relationship Of Serum Lipids And Dietary Intake Of Fat In Senior Citizens, Lina Wu

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A number of research studies have shown that aging is a continuous and cumulative process of biological changes which frequently result in cardiovascular diseases in the older age group. Degenerative diseases of old age such as atherosclerosis and coronary artery diseases appear to be related to abnormal lipid metabolism. The findings which indicate serum cholesterol values rise with age are by no means unanimous. The combined evidence in the literature points out that serum cholesterol of newborn infants is low, then rises steadily with age in both sexes from adolescence through middle age with a higher value in males than …

Neutral 17-Ketosteroids And 17, 21-Dihydroxy-20-Ketosteroids In Urine Of University Students, Shon-Hua Yu

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Degenerative disease of old age such as atherosclerosis and cerebral hemorrhage appear to be related to abnormal lipid metabolism. Many investigators have studied the role of steroid hormones on lipid and cholesterol metabolism in rats and chickens. The results have consistantly indicated that both corticosteroids and sex hormones play an important role in the regulation of lipid and cholesterol metabolism, in vivo. Serum cholesterol values in humans have been decreased by estrogens, while androgen administration tends to increase circulating cholesterol. Estrogens have been shown to decrease circulating cholesterol and prevent cholesterol-induced coronary atherosclerosis in chickens. A study of the …