Molecular and Cellular Neuroscience Commons^™

Distributions Of Hypothalamic Neuron Populations Co-Expressing Tyrosine Hydroxylase And The Vesicular Gaba Transporter In The Mouse., Kenichiro Negishi, Mikayla A. Payant, Kayla S. Schumacker, Gabor Wittman, Rebecca M. Butler, Ronald M. Lechan, Harry W. M. Steinbusch M, Arshad M. Khan, Melissa J. Chee

Arshad M. Khan, Ph.D.

No abstract provided.

Mapping Molecular Datasets Back To The Brain Regions They Are Extracted From: Remembering The Native Countries Of Hypothalamic Expatriates And Refugees, Arshad M. Khan, Alice H. Grant, Anais Martinez, Gully Apc Burns, Brendan S. Thatcher, Vishwanath T. Anekonda, Benjamin W. Thompson, Zachary S. Roberts, Daniel H. Moralejo, James E. Blevins

Arshad M. Khan, Ph.D.

Microarray Analysis Of Aging-Associated Immune System Alterations In The Rostral Ventrolateral Medulla Of F344 Rats, Sivasai Balivada, Chanran K. Ganta, Yongqing Zhang, Hitesh N. Pawar, Richard J. Ortiz, Kevin G. Becker, Arshad M. Khan, Michael J. Kenney

Arshad M. Khan, Ph.D.

Whole Genome Dna Methylation Sequencing Of The Chicken Retina, Cornea And Brain, Isac Lee, Bejan A. Rasoul, Ashton S. Holub, Alannah Lejeune, Raymond A. Enke, Winston Timp

Ray Enke Ph.D.

No abstract provided.

Rna Sequencing Analysis Of The Developing Chicken Retina, Christophe Langouet-Astrie*, Annamarie Meinsen*, Emily R. Grunwald*, Stephen Turner, Raymond A. Enke

Ray Enke Ph.D.

Therapeutic Raavrh10 Mediated Sod1 Silencing In Adult Sod1(G93a) Mice And Nonhuman Primates, Florie Borel, Gwladys Gernoux, Brynn Cardozo, Jake P. Metterville, Gabriela Toro Cabrera, Lina Song, Qin Su, Guang Ping Gao, Mai K. Elmallah, Robert H. Brown Jr., Christian Mueller

Christian Mueller

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease; survival in ALS is typically 3-5 years. No treatment extends patient survival by more than three months. Approximately 20% of familial ALS and 1-3% of sporadic ALS patients carry a mutation in the gene encoding superoxide dismutase 1 (SOD1). In a transgenic ALS mouse model expressing the mutant SOD1(G93A) protein, silencing the SOD1 gene prolongs survival. One study reports a therapeutic effect of silencing the SOD1 gene in systemically treated adult ALS mice; this was achieved with a short hairpin RNA, a silencing molecule that has raised multiple safety concerns, and …

Keynote Speaker Presentations: 5th Annual Umass Center For Clinical And Translational Research Retreat (Video), Robert H. Brown Jr., Thomas Grisso

Thomas Grisso

This video features the full keynote presentations from the 5th Annual UMass Center for Clinical and Translational Science Research Retreat at the University of Massachusetts Medical School (UMMS) in Worcester, MA, on May 20, 2014.

Beginning at 12:40

1st Keynote Speaker: Robert H. Brown, Jr., MD, D.Phil, Chair, Department of Neurology, UMMS. “Lou Gehrig Disease: From Mapping to Medicines”

Beginning at 1:22:19

2nd Keynote Speaker: Thomas Grisso, PhD, Director, Law and Psychiatry Program and Professor, Department of Psychiatry, UMMS. Recipient, Chancellor’s Medal for Distinguished Scholarship. “Translational Research in Law and Psychiatry”

Also included is a brief introductory presentation with updates …

Role Of Gluk1 Kainate Receptors In Seizures, Epileptic Discharges, And Epileptogenesis, Brita Fritsch, Janine Reis, Maciej Gasior, Rafal M. Kaminski, Michael A. Rogawski

Michael A. Rogawski

Kainate receptors containing the GluK1 subunit have an impact on excitatory and inhibitory neurotransmission in brain regions, such as the amygdala and hippocampus, which are relevant to seizures and epilepsy. Here we used 2-amino-3-(3-hydroxy-5-tert-butylisoxazol-4-yl) propanoic acid (ATPA), a potent and selective agonist of kainate receptors that include the GluK1 subunit, in conjunction with mice deficient in GluK1 and GluK2 kainate receptor subunits to assess the role of GluK1 kainate receptors in provoking seizures and in kindling epileptogenesis. We found that systemic ATPA, acting specifically via GluK1 kainate receptors, causes locomotor arrest and forelimb extension (a unique behavioral characteristic of GluK1 …

Jmu 4-Va Grant: Gene Expression Analysis In The Developing Vertebrate Retina Using Next Generation Sequencing, Raymond A. Enke

Ray Enke Ph.D.

Jmu 4-Va Grant: Characterizing Epigenetic Regulation Of Gene Experession During Development Of The Vertebrate Retina, Raymond A. Enke

Ray Enke Ph.D.

Enhanced Nicotine Self-Administration And Suppressed Dopaminergic Systems In A Rat Model Of Diabetes, Laura O'Dell

Laura Elena O'Dell

Patients with diabetes display a heightened propensity to use tobacco; however, it is unclear whether they experience enhanced rewarding effects of nicotine. Thus, this study examined the reinforcing effects of nicotine in a rodent model of diabetes involving administration of streptozotocin (STZ), a drug that is toxic to pancreatic insulin-producing cells. The first study compared STZ- and vehicle-treated rats that had 23-hour access to intravenous self-administration (IVSA) of nicotine or saline and concomitant access to food and water. In order to examine the contribution of dopamine to our behavioral effects, dopamine transporter (DAT), D1 and D2 receptor levels were compared …

Effects On Pharmacological Properties Of Chimeric Α6Β2Β3* Achrs With Α3 Cytoplasmic Domain(S) And The Ml (Methionine To Leucine) Mutation At Position 211 Of Α6, Carson K. Ley

Carson K Ley

α6β2β3* acetylcholine receptors (AChRs) on dopaminergic neurons located in the brain are critical targets for drugs to treat nicotine addiction and Parkinson's disease. a6 and a3 are acetylcholine receptor (AChR) subunits closely related in sequence. a3 assembles efficiently with b2 subunits to form functional AChRs in Xenopus laevis oocytes, but a6 does not, presumably because specific chaperones needed for efficient expression are missing (Gotti et al., 2010). Chimeras of a6 with a3 assemble efficiently to form functional AChRs . To be useful and not misleading, it is vital that the chimeras incorporate only enough a3 sequence to insure efficient assembly …

Application Of Three-Dimensional Culture Conditions To Human Embryonic Stem Cell-Derived Definitive Endoderm Cells Enhances Hepatocyte Differentiation And Functionality, Thamil Selvee Ramasamy

Thamil Selvee Ramasamy

Human embryonic stemcells (hESCs) and induced pluripotent stemcells (iPSCs) provide an unlimited source for the generation of human hepatocytes, owing to their indefinite self-renewal and pluripotent properties. Both hESC-/iPSC-derived hepatocytes hold great promise in treating liver diseases as potential candidates for cell replacement therapies or as an in vitro platform to conduct newdrug trials. It has been previously demonstrated that the initiation of hESC differentiation in monolayer cultures increases the generation of definitive endoderm (DE) and subsequently of hepatocyte differentiation. However, monolayer culture may hinder the maturation of hESC-derived hepatocytes, since such two-dimensional (2D) conditions do not accurately reflect the …

Retinal Ganglion Cell Differentiation And Transplantation, Jonathan Hertz

Jonathan Hertz

Adult central nervous system (CNS) neurons fail to regenerate following injury, and there is no repair or replacement of cells lost after injury or in neurodegenerative diseases. There is much interest in transplanting stem cell-derived neurons into the injured nervous system and enhancing the differentiation of donor cells into mature, integrated and functional neurons. Little is known, however, about what signals control the differentiation and integration of neurons, either during development or in the adult. Generating appropriate types of donor neurons from stem cells has been challenging because the signals that regulate neural subtype-specific fates are largely unknown. Therefore, it …

Conditional Knockdown Of Dna Methyltransferase-1 (Dnmt1) Reveals A Key Role Of Retinal Pigment Epithelium In Mammalian Photoreceptor Differentiation, I. O. Nasonkin, S. L. Merbs, K. Lazo, V. F. Oliver, M. Brooks, K. Patel, Raymond Enke, J. Nellissery, M. Jamrich, Y. Z. Le, K. Bharti, R. N. Fariss, R. A. Rachel, D. J. Zack, E. Rodriguez-Boulan, A. Swaroop

Ray Enke Ph.D.

No abstract provided.

Step Regulation Of Seizure Thresholds In The Hippocampus, Janice R. Naegele

Janice R Naegele

Purpose:  To investigate whether striatal enriched protein tyrosine phosphatase (STEP) influences ictogenesis. Methods:  STEP knockout mice were compared to wild-type (WT) mice in pilocarpine-induced seizures. Hippocampal slices were also prepared from these two mouse populations, allowing the examination of ictal-like stimulation in these slices using calcium imaging and electrophysiologic recordings. Key Findings:  To examine seizure thresholds, increasing doses of pilocarpine were administered to adult mice and seizures were scored behaviorally. Significantly fewer STEP knockout mice developed seizures that progressed to the stage of status epilepticus compared to WT mice. To examine potential differences in neural circuits that might account for …

Tangled Roots: Digging Deeper Into Astrocyte Or Interneuron Dysfunction In Temporal Lobe Epilepsy., Janice R. Naegele

Janice R Naegele

No abstract provided.

Effect Of Nicotine On Body Composition, Laura O'Dell

Laura Elena O'Dell

No abstract provided.

Flatbed Scanner Report - Optical Density Dynamic Range, George Mcnamara

George McNamara

George McNamara (now at University of Miami) report for Hua Yu and Richard Jove, City of Hope National Medical Center, on optical density dynamic range of several flatbed scanners.

Roles Of The Ion Channel Nalcn In Neuronal Excitability Control, Boxun Lu

Boxun Lu

The resting membrane potential (RMP) of a neuron is set by a complex balance between charged ions, ion channels and transporters. Many of the ion channels have been identified at the molecular level. Missing from the molecular identification has been the voltage-insensitive background sodium ‘‘leak’’ conductance that depolarizes the RMP from the equilibrium potential of potassium and provides a crucial contribution to neuronal excitability One candidate for the molecular identity of this conductance is the protein NALCN. NALCN is a previously uncharacterized orphan member in the sodium/calcium channel family. It is widely expressed in the nervous system. My thesis project …

Introduction To Nanoscopy Nano-Talk, George Mcnamara

George McNamara

T7-1 is the designation for the LMRG Nanoscopy session at ABRF in Orlando, FL, on March 20, 2012. The PDF file here is a draft of my presentation.

May not be very helpful since (1) would probably help to know what is in my head and each slide will [hopefully] prompt me to say, and (2) 10 minute talk so I am going to push the "next slide" button after saying very little.

__________________

Publisher statement:

The T7-1 Introduction to Nanoscopy Nano Talk is copyrighted (c) George McNamara, 2012. Except for (1) screenshots from research articles (which are copyrighted by …

Rogers Pmn Movie - Background Information, George Mcnamara

George McNamara

Please see

http://mdc.custhelp.com/app/answers/detail/a_id/18689/~/metamatters-newsletters

for my series of MetaMorph MetaMatters articles in volume 2, numbers 3 through 6, on the Rogers PMN Panorama data set.

http://mdc.custhelp.com/app/answers/detail/a_id/18689/~/metamatters-newsletters

Brief summary (for more, see the Word doc)

Map Kinases Couple Hindbrain-Derived Catecholamine Signals To Hypothalamic Adrenocortical Control Mechanisms During Glycemia-Related Challenges, Arshad M. Khan, Kimberly L. Kaminski, Graciela Sanchez-Watts, Todd A. Ponzio, J. Brent Kuzmiski, Jaideep S. Bains, Alan G. Watts

Arshad M. Khan, Ph.D.

No abstract provided.

Mcnamara 2011 Mpmicro - Multi-Probe Microscopy (10/31/2011), George Mcnamara

George McNamara

Multi-Probe Microscopy is an ~1500 page Word document summarizing what I know and/or found interesting in light microscopy, fluorescence microscopy and digital image analysis, from 1995-2005. Very little has been updated since 2005.

Nico-Teen: Neural Substrates That Mediate Adolescent Tobacco Abuse., Laura O'Dell

Laura Elena O'Dell

No abstract provided.

Nico-Teen: Neural Substrates That Mediate Adolescent Tobacco Abuse., Laura O'Dell

Laura Elena O'Dell

No abstract provided.

Impact Of Serotonin 2c Receptor Null Mutation On Physiology And Behavior Associated With Nigrostriatal Dopamine Pathway Function., Laura O'Dell

Laura Elena O'Dell

No abstract provided.

Alcohol Self-Administration Acutely Stimulates The Hypothalamic-Pituitary-Adrenal (Hpa) Axis, But Alcohol Dependence Leads To A Dampened Neuroendocrine State, Laura O'Dell

Laura Elena O'Dell

No abstract provided.

Female Rats Display Dose-Dependent Differences To The Rewarding And Aversive Effects Of Nicotine In An Age-, Hormone-, And Sex-Dependent Manner., Laura O'Dell

Laura Elena O'Dell

No abstract provided.

Rodent Models Of Nicotine Reward: What Do They Tell Us About Tobacco Abuse In Humans?, Laura O'Dell

Laura Elena O'Dell

No abstract provided.