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Molecular and Cellular Neuroscience Commons™

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Developmental Neuroscience

Selected Works

Articles 1 - 13 of 13

Full-Text Articles in Molecular and Cellular Neuroscience

Micrornas Are Necessary For Bmp-7-Induced Dendritic Growth In Cultured Rat Sympathetic Neurons, Vidya Chandrasekaran Sep 2019

Micrornas Are Necessary For Bmp-7-Induced Dendritic Growth In Cultured Rat Sympathetic Neurons, Vidya Chandrasekaran

Vidya Chandrasekaran

Neuronal connectivity is dependent on size and shape of the dendritic arbor. However, mechanisms controlling dendritic arborization, especially in the peripheral nervous system, are not completely understood. Previous studies have shown that bone morphogenetic proteins (BMPs) are important initiators of dendritic growth in peripheral neurons. In this study, we examined the hypothesis that post-transcriptional regulation mediated by microRNAs (miRNAs) is necessary for BMP-7-induced dendritic growth in these neurons. To examine the role of miRNAs in BMP-7-induced dendritic growth, microarray analyses was used to profile miRNA expression in cultured sympathetic neurons from the superior cervical ganglia of embryonic day 21 rat pups at 6 and 24 h after treatment with BMP-7 (50 ng/mL). Our data showed that BMP-7 significantly …

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Mapping Molecular Datasets Back To The Brain Regions They Are Extracted From: Remembering The Native Countries Of Hypothalamic Expatriates And Refugees, Arshad M. Khan, Alice H. Grant, Anais Martinez, Gully Apc Burns, Brendan S. Thatcher, Vishwanath T. Anekonda, Benjamin W. Thompson, Zachary S. Roberts, Daniel H. Moralejo, James E. Blevins Jun 2018

Mapping Molecular Datasets Back To The Brain Regions They Are Extracted From: Remembering The Native Countries Of Hypothalamic Expatriates And Refugees, Arshad M. Khan, Alice H. Grant, Anais Martinez, Gully Apc Burns, Brendan S. Thatcher, Vishwanath T. Anekonda, Benjamin W. Thompson, Zachary S. Roberts, Daniel H. Moralejo, James E. Blevins

Arshad M. Khan, Ph.D.

This article, which includes novel unpublished data along with commentary and analysis,
focuses on approaches to link transcriptomic, proteomic, and peptidomic datasets mined from
brain tissue to the original locations within the brain that they are derived from using digital atlas
mapping techniques. We use, as an example, the transcriptomic, proteomic and peptidomic
analyses conducted in the mammalian hypothalamus. Following a brief historical overview, we
highlight studies that have mined biochemical and molecular information from the hypothalamus
and then lay out a strategy for how these data can be linked spatially to the mapped locations in a
canonical brain atlas …

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Pleiotropic And Isoform-Specific Functions For Pitx2 In Superior Colliculus And Hypothalamic Neuronal Development, Mindy Waite Dec 2012

Pleiotropic And Isoform-Specific Functions For Pitx2 In Superior Colliculus And Hypothalamic Neuronal Development, Mindy Waite

Mindy Waite

Transcriptional regulation of gene expression during development is critical for proper neuronal differentiation and migration. Alternative splicing and differential isoform expression have been demonstrated for most mammalian genes, but their specific contributions to gene function are not well understood. In mice, the transcription factor gene Pitx2 is expressed as three different isoforms (PITX2A, PITX2B, and PITX2C) which have unique amino termini and common DNA binding homeodomains and carboxyl termini. The specific roles of these isoforms in neuronal development are not known. Here we report the onset of Pitx2ab and Pitx2c isoform-specific expression by E9.5 in the developing mouse brain. Using …

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Retinal Ganglion Cell Differentiation And Transplantation, Jonathan Hertz Dec 2012

Retinal Ganglion Cell Differentiation And Transplantation, Jonathan Hertz

Jonathan Hertz

Adult central nervous system (CNS) neurons fail to regenerate following injury, and there is no repair or replacement of cells lost after injury or in neurodegenerative diseases. There is much interest in transplanting stem cell-derived neurons into the injured nervous system and enhancing the differentiation of donor cells into mature, integrated and functional neurons. Little is known, however, about what signals control the differentiation and integration of neurons, either during development or in the adult. Generating appropriate types of donor neurons from stem cells has been challenging because the signals that regulate neural subtype-specific fates are largely unknown. Therefore, it …

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Effect Of Nicotine On Body Composition, Laura O'Dell May 2012

Effect Of Nicotine On Body Composition, Laura O'Dell

Laura Elena O'Dell

No abstract provided.

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A Novel Taulacz Allele Reveals A Requirement For Pitx2 In Formation Of The Mammillothalamic Tract, Mindy Waite Dec 2011

A Novel Taulacz Allele Reveals A Requirement For Pitx2 In Formation Of The Mammillothalamic Tract, Mindy Waite

Mindy Waite

The hypothalamic mammillary region is critical for spatial memory and vestibular processing. Pitx2 encodes a paired-like transcription factor that is highly expressed in the developing mammillary region and is required for subthalamic nucleus formation. Here we analyzed a loss of function Pitx2-TaulacZ knock-in allele to study the effects of Pitx2 deficiency on neuronal projections in the embryonic mammillary region. Pitx2-expressing neurons contribute axons to principal mammillary, mammillotegmental and mammillotectal tracts. Embryos with Pitx2 deficiency exhibit axonal fibers in the principal mammillary tract that are improperly bundled and disorganized, yet project caudally toward the tectum and tegmentum. Embryos …

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Distinct Populations Of Gabaergic Neurons In Mouse Rhombomere 1 Express But Do Not Require The Homeodomain Transcription Factor Pitx2, Mindy Waite Dec 2011

Distinct Populations Of Gabaergic Neurons In Mouse Rhombomere 1 Express But Do Not Require The Homeodomain Transcription Factor Pitx2, Mindy Waite

Mindy Waite

Hindbrain rhombomere 1 (r1) is located caudal to the isthmus, a critical organizer region, and rostral to rhombomere 2 in the developing mouse brain. Dorsal r1 gives rise to the cerebellum, locus coeruleus, and several brainstem nuclei, whereas cells from ventral r1 contribute to the trochlear and trigeminal nuclei as well as serotonergic and GABAergic neurons of the dorsal raphe. Recent studies have identified several molecular events controlling dorsal r1 development. In contrast, very little is known about ventral r1 gene expression and the genetic mechanisms regulating its formation. Neurons with distinct neurotransmitter phenotypes have been identified in ventral r1 …

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Gabaergic And Glutamatergic Identities Of Developing Midbrain Pitx2 Neurons, Mindy Waite Jan 2011

Gabaergic And Glutamatergic Identities Of Developing Midbrain Pitx2 Neurons, Mindy Waite

Mindy Waite

Pitx2, a paired-like homeodomain transcription factor, is expressed in post-mitotic neurons within highly restricted domains of the embryonic mouse brain. Previous reports identified critical roles for PITX2 in histogenesis of the hypothalamus and midbrain, but the cellular identities of PITX2-positive neurons in these regions were not fully explored. This study characterizes Pitx2 expression with respect to midbrain transcription factor and neurotransmitter phenotypes in mid-to-late mouse gestation. In the dorsal midbrain, we identified Pitx2-positive neurons in the stratum griseum intermedium (SGI) as GABAergic and observed a requirement for PITX2 in GABAergic differentiation. We also identified two Pitx2-positive neuronal populations in the …

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Nico-Teen: Neural Substrates That Mediate Adolescent Tobacco Abuse., Laura O'Dell Dec 2010

Nico-Teen: Neural Substrates That Mediate Adolescent Tobacco Abuse., Laura O'Dell

Laura Elena O'Dell

No abstract provided.

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Nico-Teen: Neural Substrates That Mediate Adolescent Tobacco Abuse., Laura O'Dell Dec 2010

Nico-Teen: Neural Substrates That Mediate Adolescent Tobacco Abuse., Laura O'Dell

Laura Elena O'Dell

No abstract provided.

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Protracted Withdrawal From Alcohol And Drugs Of Abuse Impairs Long-Term Potentiation Of Intrinsic Excitability In The Juxtacapsular Bed Nucleus Of The Stria Terminalis, Laura O'Dell Dec 2008

Protracted Withdrawal From Alcohol And Drugs Of Abuse Impairs Long-Term Potentiation Of Intrinsic Excitability In The Juxtacapsular Bed Nucleus Of The Stria Terminalis, Laura O'Dell

Laura Elena O'Dell

No abstract provided.

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The Effects Of Opioids And Opioid Analogs On Animal And Human Endocrine Systems, Laura O'Dell Dec 2008

The Effects Of Opioids And Opioid Analogs On Animal And Human Endocrine Systems, Laura O'Dell

Laura Elena O'Dell

No abstract provided.

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Pdf Of All O'Dell Manuscripts, Laura O'Dell Dec 2008

Pdf Of All O'Dell Manuscripts, Laura O'Dell

Laura Elena O'Dell

No abstract provided.

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