Neuroscience and Neurobiology Commons^™

The Neonatal Anti-Viral Response Fails To Control Measles Virus Spread In Neurons Despite Interferon-Gamma Expression And A Th1-Like Cytokine Profile, Priya Ganesan

Electronic Theses and Dissertations

Neonates are highly susceptible to infections in the central nervous system (CNS) and have a greater risk of viral infections and encephalopathies. Neurotropic viral infections can lead to blindness, hearing loss and neurological deficiencies such as cognitive impairment, epilepsy, and even death in the neonatal and pediatric populations. Viral infections also are hypothesized to indirectly contribute to neurodegenerative and neuropsychiatric diseases such as Schizophrenia and Parkinson’s disease later in life due to early neuronal damage or stress. Many diverse viruses are capable of invading the neonatal CNS including Borna Disease Virus, Coxsackievirus (CV), Herpes simplex viruses (HSV), and measles virus. …

A Behavioral And Neuroimmune System Model Of The Effects Of Chronic Low-Level Lead Exposure In Young Male C57bl/6j Mice, Mayra Gisel Flores-Montoya

Open Access Theses & Dissertations

Chronic low-level lead exposure reduces memory in children however the brain mechanisms mediating these effects are not known. In previous studies we showed that early lead exposure reduced olfactory memory and exploratory behavior in young mice, and reduced microglia cell density in hippocampus/dentate gyrus. The present studies aimed to identify additional behavioral tests that were sensitive to early low-level lead exposure in young mice; and to examine whether microglia upregulated factors known to promote cell migration. Seventy-two C57BL/6J male mice were exposed to 0 ppm (controls), 30 ppm (low-dose), or 430 ppm (high-dose) of lead acetate via dams' milk from …

To Identify All The Relevant Factors Is To Explain Feeling, Arthur S. Reber

Animal Sentience

Several additional comments on Reber (2016a) have appeared. Like those addressed in Reber (2016b), they reflect points of agreement and disagreement on various elements of my Cellular Basis of Consciousness (CBC) model. Some, however, seem to have missed key points. I'm willing to take some responsibility for this. Perhaps I was not clear about some of the more radical points of the model. Hopefully the case-by-case review here will help.

Consciousness, Evidence, And Moral Standing, Irina Mikhalevich

Animal Sentience

Woodruff (2017) claims to have identified the neural correlates of phenomenal consciousness (“p-consciousness”) in fishes, and argues that these neurological data, along with behavioral evidence, suggest that teleost fishes are in all probability sentient organisms. Woodruff’s case may be strengthened by challenging key assumptions behind a common criticism of accounts such as his: that fishes cannot be p-conscious because they lack the cortical structures necessary for p-consciousness. A more serious objection to Woodruff’s proposal would be that his evidence for p-consciousness establishes only that fishes are “access-conscious” (“a-conscious”), where a-conscious states are cognitive representations that are made available to cognitive …

Quantification Of Brain-Region Specific Alterations In Insulin-Like Growth Factor-1 Expression During Developmental Hypothyroidism, Cari Lee Graber-Feesl

All Graduate Theses, Dissertations, and Other Capstone Projects

Insufficient thyroid hormone (TH) during development results in permanent neurological deficits. These deficits are the result of perturbed TH-mediated brain development. Interestingly, insufficient insulin-like growth factor 1 (Igf-1) during development results in neurological deficits that are similar to those reported for developmental hypothyroidism. This observation suggests that deficits associated with low TH during development may be the result of altered Igf-1 expression in the developing brain. To test this, timed-pregnant mice were treated with thyroid gland inhibitors from gestational day 16 (GD16) until postnatal day 21 (P21) to induce a hypothyroid state. A parallel set of untreated timed-pregnant mice were …

The Default Mode Network And Executive Function: Influence Of Age, White Matter Connectivity, And Alzheimer’S Pathology, Christopher A. Brown

Theses and Dissertations--Neuroscience

The default mode network (DMN) consists of a set of interconnected brain regions supporting autobiographical memory, our concept of the self, and the internal monologue. These processes must be maintained at all times and consume the highest amount of the brain’s energy during its baseline state. However, when faced with an active, externally-directed cognitive task, the DMN shows a small, but significant, decrease in activity. The reduction in DMN activity during the performance of an active, externally-directed task compared to a baseline state is termed task-induced deactivation (TID), which is thought to ‘free-up’ resources required to respond to external demands. …

Neuropathological And Genetic Correlates Of Survival And Dementia Onset In Synucleinopathies: A Retrospective Analysis, David J. Irwin, Murray Grossman, Daniel Weintraub, Howard I. Hurtig, John E. Duda, Sharon X. Xie, Edward B. Lee, Vivianna M. Van Deerlin, Oscar L. Lopez, Julia K. Kofler, Peter T. Nelson, Gregory A. Jicha, Randy Woltjer, Joseph F. Quinn, Jeffery Kaye, James B. Leverenz, Debby Tsuang, Katelan Longfellow, Dora Yearout, Walter Kukull, C. Dirk Keene, Thomas J. Montine, Cyrus P. Zabetian, John Q. Trojanowski

Sanders-Brown Center on Aging Faculty Publications

Background

Great heterogeneity exists in survival and the interval between onset of motor symptoms and dementia symptoms across synucleinopathies. We aimed to identify genetic and pathological markers that have the strongest association with these features of clinical heterogeneity in synucleinopathies.

Methods

In this retrospective study, we examined symptom onset, and genetic and neuropathological data from a cohort of patients with Lewy body disorders with autopsy-confirmed α synucleinopathy (as of Oct 1, 2015) who were previously included in other studies from five academic institutions in five cities in the USA. We used histopathology techniques and markers to assess the burden of …

Immunotherapy Against Drugs Of Abuse, Gianna Raimondi (Class Of 2017)

Writing Across the Curriculum

Current treatments for drug addiction involve classical pharmacological therapy, involving the use of competitive or noncompetitive agonists (full, partial, or inverse) and antagonists. Drugs of abuse enter the brain after crossing the blood-brain barrier rapidly and binding to the proper receptor(s). They are able to do so because they are small and lipid soluble, and produce reinforcing effects by increasing levels of dopamine in brain areas associated with reward. This occurs in specific systems associated with addiction. In the mesolimbic system, neuron cell bodies originate in the ventral tegmental area (VTA) and project to the nucleus accumbens (NAc), amygdala, hippocampus, …

Mitochondrial Transplantation After Spinal Cord Injury: Effects On Tissue Bioenergetics And Functional Neuroprotection, Jenna L. Gollihue

Theses and Dissertations--Physiology

Contusion spinal cord injury (SCI) results in devastating life-long debilitation in which there are currently no effective treatments. The primary injury site presents a complex environment marked by subsequent secondary pathophysiological cascades involving excessive reactive oxygen and nitrogen species (ROS/RNS) production, glutamate-induced excitotoxicity, calcium dysregulation, and delayed neuronal apoptosis. Many of these cascades involve mitochondrial dysfunction, thus a single mitochondrial-centric therapy that targets a variety of these factors could be far reaching in its potential benefits after SCI. As such, this dissertation examines whether transplantation of exogenous mitochondria after SCI can attenuate secondary injury cascades to decrease the spread and …

What Harmful Practices? The Material Scope Of Animal Protection Legislation, Eze Paez

Animal Sentience

Jonathan Birch proposes a criterion for the subjective scope of animal protection legislation. He says nothing about its material scope: which harmful practices it should regulate. I argue, first, that most moral views would agree that the worst forms of animal exploitation should be legally forbidden, even if there will inevitably be disagreement about some cases of animal experimentation. I also argue that, when feasible, there should be legal provisions to help wild animals.

Raising The Moral Consciousness Of Science, Bernard Rollin

Animal Sentience

Precaution on behalf of sentient animals should not be tempered by the questionable principle of the amorality of science.

Consciousness Is Not Inherent In But Emergent From Life, Jon Mallatt, Todd E. Feinberg

Animal Sentience

Reber’s theory of the cellular basis of consciousness (CBC) is right to emphasize that we should study consciousness (sentience) in its simplest form, taking its evolution into account. However, not enough evidence is presented to support CBC’s unorthodox claim that even simple, one-celled organisms are conscious. As pointed out by other commentators, the CBC seems to be based on outdated ideas about evolution and does not acknowledge that consciousness could be an evolutionary novel feature. Such emergent features are abundant in living organisms. We review our own emergentist solution, in which consciousness evolved in the elaborating nervous systems of the …

Bladder Antimuscarinics And Cognitive Decline In Elderly Patients, Daniela Claudia Moga, Erin L. Abner, Qishan Wu, Gregory A. Jicha

Pharmacy Practice and Science Faculty Publications

Introduction: The evidence on the impact of bladder antimuscarinics initiation on cognitive function in older adults is inconsistent.

Methods: A retrospective analysis of data from the National Alzheimer's Coordinating Center (NACC) on enrollees 65 years and older evaluated the association between antimuscarinic initiation and cognitive decline. We defined decline from baseline (yes/no) for cognitive assessments included in the NACC Uniform Data Set 2.0 battery. New users were matched on year of enrollment and time in the cohort to randomly selected nonusers. Analyses were conducted using inverse probability of treatment weights based on baseline propensity scores.

Results: Our analyses included 698 …

Effects Of Chromium On Mouse Splenic T Lymphocytes And Effects Of Ethanol Exposure During Early Neurodevelopment On Behaviors In Mice, Lu Dai

Theses and Dissertations--Toxicology and Cancer Biology

The dissertation consists of three major projects with the focus on the immunotoxicity of chromium and the behavior disorders caused by early ETOH exposure respectively.

Hexavalent chromium [Cr(VI)] is widely used in various industrial processes and has been recognized as a carcinogen. As the first line of host defense system, the immune system can be a primary target of Cr(VI). T cell population represents a major arm of the immune system that plays a critical role in host anti-tumor immunity. Dysfunction of T cells compromises host anti-tumor immunity resulting in oncogenesis. Using mouse splenic T cells as an in vitro …

Dogs Consciously Experience Emotions: The Question Is, Which?, Ralph Adolphs

Animal Sentience

I discuss three themes related to Kujala’s target article. First, the wealth of emerging data on cognitive studies in dogs will surely show that dogs have a very rich repertoire of cognitive processes, for most of which we find homologues in humans. Second, understanding the internal states that mediate social behaviors, such as emotions, requires us to consider both a dog’s behaviors with other dogs, and the emergence of new behavioral patterns in interaction with humans. Third, all of this will certainly narrow the range of justifications for denying that dogs have subjective experiences of emotions.

Changes In Postural Sway Behavior Across The Life Span, Maria M B N R Santos

Graduate Student Theses, Dissertations, & Professional Papers

The present study aimed to investigate human balance control by assessing postural sway on three groups representing three stages of life (6-12, 19-40 and 65-74 years old). There were 14 individuals in each group and they were tested during upright bipedal stance with either eyes open or closed. Focus was given to multiple sway indices representing multi-dimensional features of postural sway in quiet stance and included: the center of pressure area, amplitude, root mean square (RMS), velocity, jerkiness, and sample entropy. Results confirmed that children and seniors swayed more (p<.004), faster (p<.001) and their body sway was shakier (p<.001) than young adults. Seniors also presented faster (p<.006) and shakier (p<.001) sway than children and a more unpredictable pattern of body sway in time (p<.002) than children and young adults. In addition, children presented a more random anterior-posterior sway (p<.034) and a more regular medio-lateral sway (p<.043) than young adults, and a higher synchronization between anterior-posterior and medio-lateral body sway (p<.012) than young adults and seniors. We also observed that postural control of children and young adults becomes relatively more challenged in experimental situations when eyes were closed for most postural indices. In conclusion, this study suggests that multi-dimension posturography is sensitive to detect subtle age-related changes in the postural behavior and each stage of life may have their own signature patterns of postural behavior. Therefore, we expect that quantifications of this nature may be used to assess not only postural instability and fall risk but also to aid the testing of the efficacy of balance interventional protocols.

Of Cortex And Consciousness: “Phenomenal,” “Access,” Or Otherwise, Scott A. Husband

Animal Sentience

From the perspective of a comparative neuroanatomist studying the avian pallium, Woodruff’s (2017) claims about the behavioral and electrophysiological evidence for teleost sentience blur the lines between phenomenal and access consciousness (Block, 1995). I discuss the bias that complex cognition can only arise in the cortical layering typical of the mammalian pallium and conclude that Woodruff makes a good case that the tecto-pallial connections in teleosts are sufficiently complex to support something like sentience.

Identification Of Novel Sleep Related Genes From Large Scale Phenotyping Experiments In Mice, Shreyas Joshi

Theses and Dissertations--Biology

Humans spend a third of their lives sleeping but very little is known about the physiological and genetic mechanisms controlling sleep. Increased data from sleep phenotyping studies in mouse and other species, genetic crosses, and gene expression databases can all help improve our understanding of the process. Here, we present analysis of our own sleep data from the large-scale phenotyping program at The Jackson Laboratory (JAX), to identify the best gene candidates and phenotype predictors for influencing sleep traits.

The original knockout mouse project (KOMP) was a worldwide collaborative effort to produce embryonic stem (ES) cell lines with one of …

The Neuromodulatory Mechanisms That Monoamines Control Simple And Complex Behaviors, Paul Rafael Bermeo Sabandal

Open Access Theses & Dissertations

For my Dissertation, I worked on three projects in the fruit fly Drosophila melanogaster, which is a well-established genetic animal model. The first project (Chapter 1) is to elucidate the neural, cellular and molecular mechanisms underlying behavioral control named response inhibition. In this study, I identified that a particular subset of dopamine neurons, D1 dopamine receptors and the neural substrates crucial for response inhibition. Abnormal response inhibition is associated with the mental disorders including attention deficit hyperactivity disorder (ADHD), autism, schizophrenia, obsessive compulsive disorder, posttraumatic stress disorder and addiction, This study may help enhance our understanding the pathogenesis mechanisms of …

4-Nonylphenol Induces Neurodegeneration By Altering Cytoskeleton, Cynthia Carreon

Open Access Theses & Dissertations

4-nonylphenol (4-NP), an endocrine-disrupting compound (EDC), has been shown to affect brain development and may cause neurodegeneration. In the environment, 4-NP arises as a degradation product of alkylphenol polyethoxylates, compounds widely used as nonionic surfactants in commercial production, as well as in herbicides, pesticides, polystyrene plastics, and paints and has been shown to undergo a high level of accumulation in biological tissues. However, the mechanism by which 4-NP exerts its effect is not understood. Recent results from our laboratory indicate that Gβγ, an important component of the G protein-signaling pathway, induces neuronal outgrowth and differentiation by modulating microtubule (MT) assembly, …

Characterization Of Sex Differences In The Reinforcing Effects Of Nicotine, Rodolfo Jesus Flores Garcia

Open Access Theses & Dissertations

It is presently unclear whether ovarian hormones, such as estradiol (E2) promote the reinforcing effects of nicotine in females. Thus, we compared extended access to nicotine intravenous self-administration (IVSA) in intact male, intact female, and OVX female rats (Study 1) as well as OVX females that received vehicle or E2 supplementation (Study 2). The E2 supplementation procedure involved a 4-day procedure involving 2 days of vehicle administration and 2 days of E2 administration. Two doses of E2 (25 or 250 ug) were assessed in separate groups of OVX females in order to examine the dose-dependent effects of this hormone on …

The Metabolically Sentient Arcuate Nucleus: A Functional, Chemoarchitectural And Connectional Study In The Adult Male Rat, Anais Martinez

Open Access Theses & Dissertations

As the obesity pandemic continues to increase, so does the incidence rate of related conditions including diabetes, cardiovascular disease, and neurodegenerative disorders (Yu and Kim 2012). At the level of the brain, the hypothalamus is a key structure for the integratration of autonomic, neuroendocrine and somatomotor functions for the control of metabolism and homeostasis. It has long been implicated as a critical player in the deregulation of an animal's energy status and in the onset of obesity, diabetes and metabolic syndrome (Elmquist et al., 1999). While many ongoing studies are aimed at preventing or treating these conditions, the underlying mechanisms …

Predatory Behavior And Neuroanatomy Of The Sessile Rotifer, Cupelopagis Vorax , Elizabeth Preza

Open Access Theses & Dissertations

Predator-prey interactions contribute to the evolution of behavioral and morphological characteristics of species. However, research on zooplankton has mainly focused on planktonic species such Mesocyclops edax (Arthropoda, Cyclopoida) and Asplanchna spp. (Rotifera, Ploima). Cupelopagis vorax (Rotifera, Collothecaceae) provides a unique model for studying the predatory behavior of a sessile species. Cupelopagis was chosen because it is the only rotifer known to exhibit rheotaxic behaviors in the presence of prey and because it undergoes indirect development where non-feeding, free-swimming larvae mature into feeding adults. The integration of behavioral techniques, immunohistochemistry, and confocal microscopy were used to provide insight as to how …

Method & Madness: Advanced Techniques For Characterizing Rat Hypothalamic Chemoarchitecture And For Modernizing Legacy Data, Claire Eugenia Wells

Open Access Theses & Dissertations

The hypothalamus is a region of the brain with exceptionally high anatomical, chemical, and functional diversity. It regulates homeostatic functions and a number of motivated behaviors impacting homeostasis, such as ingestion and reproduction; it also serves as a point of connection between the forebrain and hindbrain. An improved understanding of the chemical anatomy of this area would benefit scientists working in many neuroscientific disciplines. The work described herein contributes to a chemoarchitectural atlas of the rat hypothalamus, which will include data on the distribution of various hypothalamic neurotransmitters, and on their co-localizations and synaptic interactions. Importantly, the methodology used to …

What Can Vigilance Tell Us About Fear?, Guy Beauchamp

Animal Sentience

Animal vigilance is concerned with the monitoring of potential threats caused by predators and conspecifics. Researchers have argued that threats are part of a landscape of fear tracking the level of risk posed by predators and conspecifics. Vigilance, which is expected to vary with the level of risk, could thus be used as a measure of fear. Here, I explore the relationship between vigilance and fear caused by predators and conspecifics. The joint occurrence of vigilance and other physiological responses to fear, such as increased heart rate and stress hormone release, would bolster the idea that vigilance can be a …

Operationalizing Fear Through Understanding Vigilance, Ralph Adolphs

Animal Sentience

Beauchamp’s target article raises important questions about the features that often accompany fear. How reliable an indicator of fear is vigilance? Is it constitutive, cause, or consequence of fear? These questions force us towards a clearer definition of “fear.”

Individual Variation In Fear Behaviour, Rob Found

Animal Sentience

Individual variation in behaviour adds another layer of complexity to studies of animal responses to fear and risk, but when individual responses are consistent, and correlated with other behaviours, these patterns can provide a structure that furthers our understanding. I interpret Beauchamp’s review of vigilance and fear from the perspective of my own studies of elk personality. Wild elk responses to changing stimuli (benign or aversive) illustrate how personality is a key factor in explaining variation in vigilance and fear behavior. Personality even influences how wildlife can learn to assess risk and adjust their fear responses accordingly.

Canine Emotions As Seen Through Human Social Cognition, Miiamaaria V. Kujala

Animal Sentience

It is not possible to demonstrate that dogs (Canis familiaris) feel emotions, but the same is true for all other species, including our own. The issue must therefore be approached indirectly, using premises similar to those used with humans. Recent methodological advances in canine research reveal what dogs experience and what they derive from the emotions perceptible in others. Dogs attend to social cues, they respond appropriately to the valence of human and dog facial expressions and vocalizations of emotion, and their limbic reward regions respond to the odor of their caretakers. They behave differently according to the …

Direct Perception Of Animal Mind, Paul Morris

Animal Sentience

Kujala’s (2017) target article is ostensibly focused on how everyday folk (fail to) make sense of canine emotions. However, the theories outlined in the article apply to making sense of all aspects of the mentality of both human and non-human animals. The target article neglects the fundamental arguments surrounding the problem of other minds. I explore the relevant arguments and briefly review approaches suggesting that our everyday-life sense that both human and non-human animals are thinking, feeling, emotional beings has a secure epistemological basis.

Canine Emotions And The Bond Between Humans And Dogs, Robert G. Franklin Jr.

Animal Sentience

I explore how the findings reviewed by Kujala (2017) influence our understanding of the human-dog bond. The special social cognitive abilities of dogs may be unique among animal species, probably influenced by the circumstances surrounding canine domestication. The bond has influenced animal welfare policies, giving dogs a privileged place among animals. To determine what protections other species should have in a rational animal welfare policy, more research is needed on whether other species have similar social cognitive abilities.