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Influence Of Bovine Respiratory Syncytial Virus F Glycoprotein N-Linked Glycans On In Vitro Expression And On Antibody Responses In Balb/C Mice, Holly A. Klink, Ryan Brady, Christina L. Topliff, Kent M. Eskridge, Subramaniam Srikumaran, Clayton L. Kelling
Influence Of Bovine Respiratory Syncytial Virus F Glycoprotein N-Linked Glycans On In Vitro Expression And On Antibody Responses In Balb/C Mice, Holly A. Klink, Ryan Brady, Christina L. Topliff, Kent M. Eskridge, Subramaniam Srikumaran, Clayton L. Kelling
Nebraska Center for Virology: Faculty Publications
Bovine respiratory syncytial virus (BRSV) is an etiological component of the bovine respiratory tract disease complex. Infection with BRSV following vaccination, or re-infection following natural infection is common since protection is incomplete. The objectives of this study were to create plasmid DNA constructs encoding single or multiple N-glycosylation-site deletion BRSV fusion (F) proteins, and evaluate their expression in cell culture, and potential to induce anti-BRSV F antibody responses in BALB/ c mice. Four plasmid DNAs were constructed, each encoding 1-4 N-glycosylation-site deletions: Gly4, Gly2/4, Gly1/2/4 and Gly1/2/3/4. Each of the N-glycosylation-site deletion BRSV F proteins were expressed …