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Full-Text Articles in Virology
The Inability Of Human Immunodeficiency Virus To Infect Chimpanzee Monocytes Can Be Overcome By Serial Viral Passage In Vivo, Howard Gendelman, Garth D. Ehrlich, Lisa M. Baca, Shawn Conley, Jorge Ribas, D. Chester Kalter, Monte S. Meltzer, Bernard J. Poiesz, Peter Nara
The Inability Of Human Immunodeficiency Virus To Infect Chimpanzee Monocytes Can Be Overcome By Serial Viral Passage In Vivo, Howard Gendelman, Garth D. Ehrlich, Lisa M. Baca, Shawn Conley, Jorge Ribas, D. Chester Kalter, Monte S. Meltzer, Bernard J. Poiesz, Peter Nara
Nebraska Center for Virology: Faculty Publications
Studies of lentivirus infection in ruminants, nonhuman primates, and humans suggest that virus infection of macrophages plays a central role in the disease process. To investigate whether human immunodeficiency virus type 1 (HIV-1) can infect chimpanzee macrophages, we recovered monocytes from peripheral blood mononuclear cells of HIV-1-negative animals and inoculated these and control human monocytes with a panel of four human-passaged monocytotropic virus strains and one chimpanzee-passaged isolate. HIV-1-infected human monocytes synthesized proviral DNA, viral mRNA, p24 antigen, and progeny virions. In contrast, except for the chimpanzee-passaged HIV-1 isolate, chimpanzee monocytes failed to support HIV-1 replication when cultured under both …
Transactivation Of The Human Immunodeficiency Virus Promoter By Human Herpesvirus 6 (Hhv-6) Strains Gs And Z-29 In Primary Human T Lymphocytes And Identification Of Transactivating Hhv-6(Gs) Gene Fragments, Rebecca Horvat, Charles Wood, Steven Josephs, N. Balanchandran
Transactivation Of The Human Immunodeficiency Virus Promoter By Human Herpesvirus 6 (Hhv-6) Strains Gs And Z-29 In Primary Human T Lymphocytes And Identification Of Transactivating Hhv-6(Gs) Gene Fragments, Rebecca Horvat, Charles Wood, Steven Josephs, N. Balanchandran
Nebraska Center for Virology: Faculty Publications
Human herpesvirus 6 (HHV-6) can activate the human immunodeficiency virus (HIV) promoter and accelerate cytopathic effects in HIV-infected human T cells. This study examines the regions of the HIV promoter required for HHV-6 transactivation in a heterogeneous population of primary human T lymphocytes with or without antigenic stimulation. Two different strains of HHV-6, GS and Z29, transactivated the HIV promoter. The GS strain transactivated the promoter in both stimulated and resting T cells, while the Z29 strain increased HIV promoter activity only in stimulated T cells. Three DNA clones containing HHV-6(GS) genomic fragments transactivated the HIV promoter in cotransfected T …
Transactivation Of The Human Immunodeficiency Virus Promoter By Human Herpesvirus 6 (Hhv-6) Strains Gs And 2-29 In Primary Human T Lymphocytes And Identification Of Transactivating Hhv-6(Gs) Gene Fragments, Rebecca Horvat, Charles Wood, Steven Josephs, N. Balachandran
Transactivation Of The Human Immunodeficiency Virus Promoter By Human Herpesvirus 6 (Hhv-6) Strains Gs And 2-29 In Primary Human T Lymphocytes And Identification Of Transactivating Hhv-6(Gs) Gene Fragments, Rebecca Horvat, Charles Wood, Steven Josephs, N. Balachandran
Nebraska Center for Virology: Faculty Publications
Human herpesvirus 6 (HHV-6) can activate the human immunodeficiency virus (HIV) promoter and accelerate cytopathic effects in HIV-infected human T cells. This study examines the regions of the HIV promoter required for HHVd transactivation in a heterogeneous population of primary human T lymphocytes with or without antigenic stimulation. Two different strains of HHV-6, GS and 229, transactivated the HIV promoter. The GS strain transactivated the promoter in both stimulated and resting T cells, while the 229 strain increased HIV promoter activity only in stimulated T cells. Three DNA clones containing HHV-6(GS) genomic fragments transactivated the HIV promoter in cotransfected T …
Rapid Detection Of Bovine Viral Diarrhea Virus By Polymerase Chain Reaction, O. J. Lopez, Fernando A. Osorio, Ruben O. Donis
Rapid Detection Of Bovine Viral Diarrhea Virus By Polymerase Chain Reaction, O. J. Lopez, Fernando A. Osorio, Ruben O. Donis
Nebraska Center for Virology: Faculty Publications
The polymerase chain reaction was used to detect genomic sequences of the positive-stranded RNA of bovine viral diarrhea virus (BVDV), a member of the family Togaviridae. Using a set of 20-bp primers located within the conserved 3' region of the BVDV genome, we were able to consistently amplify a 205-bp target sequence from BVDV cDNA. BVDV RNAs from cell culture-propagated BVDV reference strains, diverse unrelated cytopathic and noncytopathic field isolates, and clinical serum samples were transcribed to cDNA by using avian myeloblastosis virus reverse transcriptase and further specifically amplified by using the polymerase chain reaction assay. The amplification assay …