Virology Commons^™

Proteomic Analysis Of Mycobacteriophage Crimd, William Moeller

Undergraduate Honors Theses

Bacteriophages represent a large portion of the biomatter on our planet, and many of them have yet to be fully characterized. Here we discuss the proteomic analysis of a particular Bacteriophage, Mycobacteriophage CrimD. This phage was discovered on the Campus of William & Mary and has had its genome characterized. We took the next logical step of proteomic analysis.

In our analyses we made use high pressure liquid chromatography paired with linear ion trap mass spectrometry to analyze the proteome of CrimD at specific time points after the infection of its host, Mycobacterium smegmatis. Additionally, we used nanospray ionization with …

An Evaluation Of Norovirus Persistence In Estuarine Water And Of Methods For Its Detection In Treated Sewage Effluent, Margaret H. Fagan

Dissertations, Theses, and Masters Projects

No abstract provided.

The Infection Dynamics Of Pav1 In The Caribbean Spiny Lobster Panulirus Argus, Caiwen Li

Dissertations, Theses, and Masters Projects

Panulirus argus Virus 1 (PaV1) is an emerging disease in Caribbean spiny lobster Panulirus argus. It is considered a threat to the lobster industry in the Florida Keys. In order to understand the infection dynamics of the PaV1 virus in the lobster host, a sensitive and specific fluorescence in situ hybridization (FISH) assay was developed for diagnosis of PaV1 in lobsters. The lower limit of detection using the 110-bp DNA probe in a dot-blot hybridization for PaV1 was 10 pg of cloned DNA template and 10 ng of genomic DNA extracted from hemolymph of diseased spiny lobster. The probe specifically …

Frna Coliphages As A Viral Indicator Of Sewage Pollution In An Estuary, David Miller Boyd

Dissertations, Theses, and Masters Projects

No abstract provided.