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Full-Text Articles in Virology
Genetic Diversity Of Mycobacteriophages And The Unique Abilities Of Cluster K, Shea A. Morris
Genetic Diversity Of Mycobacteriophages And The Unique Abilities Of Cluster K, Shea A. Morris
The Corinthian
Mycobacteriophage are a diverse set of viruses that infect a broad host range of mycobacterium. Genome analysis of this phage group reveals its diversity and provides evidence that they have experienced substantial horizontal gene transfer Collectively they have a large range of host bacteria they can infect, although each viral type may have a limited host range. However, this range is easily expanded and constantly changing in nature by mutation within and between virus types. These phages can lytically destroy their host mycobacteria, and it is this viral ability that have many mycobacteriophage researchers excited about the potential that phage …
Isolation Of A Mycobacterium Virus With The Infectivity Rate Tested At Various Temperatures, Katie Wegenast
Isolation Of A Mycobacterium Virus With The Infectivity Rate Tested At Various Temperatures, Katie Wegenast
Undergraduate Theses
Isolation techniques developed by the HHMI and the SEA-PHAGES program were used to obtain a bacteriophage from a mixture of loamy clay soil. The sample was retrieved from a forest edge in central Kentucky. The isolate produced large lytic plaques on a lawn of the bacterium Mycobacterium smegmatis. The bacteriophage was purified, amplified, and analyzed using restriction digest analysis with visualization by gel electrophoresis. Restriction digest analysis suggests the virus belongs to a group known as “A”. Mycobacteriophages are placed into distinct groups based on DNA sequences comparison. The next step in the procedure will be to document with …