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Full-Text Articles in Virology
Influence Of The Ccr2-V64i Polymorphism On Human Immunodeficiency Virus Type 1 Coreceptor Activity And On Chemokine Receptor Function Of Ccr2b, Ccr3, Ccr5, And Cxcr4, Benhur Lee, Benjamin J. Doranz, Shalini Rana, Yanji Yi, Mario Mellado, Jose M. R. Frade, Carlos Martinez-A., Stephen J. O'Brien, Michael Dean, Ronald G. Collman, Robert W. Doms
Influence Of The Ccr2-V64i Polymorphism On Human Immunodeficiency Virus Type 1 Coreceptor Activity And On Chemokine Receptor Function Of Ccr2b, Ccr3, Ccr5, And Cxcr4, Benhur Lee, Benjamin J. Doranz, Shalini Rana, Yanji Yi, Mario Mellado, Jose M. R. Frade, Carlos Martinez-A., Stephen J. O'Brien, Michael Dean, Ronald G. Collman, Robert W. Doms
Biology Faculty Articles
The chemokine receptors CCR5 and CXCR4 are used by human immunodeficiency virus type 1 (HIV-1) in conjunction with CD4 to infect cells. In addition, some virus strains can use alternative chemokine receptors, including CCR2b and CCR3, for infection. A polymorphism in CCR2 (CCR2-V64I) is associated with a 2- to 4-year delay in the progression to AIDS. To investigate the mechanism of this protective effect, we studied the expression of CCR2b and CCR2b-V64I, their chemokine and HIV-1 coreceptor activities, and their effects on the expression and receptor activities of the major HIV-1 coreceptors. CCR2b and CCR2b-V64I were expressed at …
Cytoplasmic Assembly And Accumulation Of Human Immunodeficiency Virus Types 1 And 2 In Recombinant Human Colony-Stimulating Factor-1-Treated Human Monocytes: An Ultrastructural Study, Jan Marc Orenstein, Monte S. Meltzer, Terri Phipps, Howard Gendelman
Cytoplasmic Assembly And Accumulation Of Human Immunodeficiency Virus Types 1 And 2 In Recombinant Human Colony-Stimulating Factor-1-Treated Human Monocytes: An Ultrastructural Study, Jan Marc Orenstein, Monte S. Meltzer, Terri Phipps, Howard Gendelman
Nebraska Center for Virology: Faculty Publications
Recombinant human colony-stimulating factor-1-treated human peripheral blood-derived monocytes-macrophages are efficient host cells for recovery of the human immunodeficiency virus (HIV) from blood leukocytes of patients with acquired immunodeficiency syndrome. These cells can be maintained as viable monolayers for intervals exceeding 3 months. Infection with HIV resulted in virus-induced cytopathic effects, accompanied by relatively high levels of released progeny virus, followed by a prolonged low-level release of virus from morphologically normal cells. In both acutely and chronically infected monocytes, viral particles were seen budding into and accumulating within cytoplasmic vacuoles. The number of intravacuolar virions far exceeded those associated with the …
Biological Characterization Of Rev Variation In Equine Infectious Anemia Virus, Michael Belshan, Matthew E. Harris, Anne E. Shoemaker, Thomas J. Hope, Susan Carpenter
Biological Characterization Of Rev Variation In Equine Infectious Anemia Virus, Michael Belshan, Matthew E. Harris, Anne E. Shoemaker, Thomas J. Hope, Susan Carpenter
Nebraska Center for Virology: Faculty Publications
Sequence analysis identified significant variation in the second exon of equine infectious anemia virus (EIAV) rev. Functional analysis indicated that limited amino acid variation in Rev significantly altered the export activity of the protein but did not affect Rev-dependent alternative splicing. EIAV Rev can mediate export through two independent cis -acting Rev-responsive elements (RREs), and differences among Rev variants were more pronounced when both RREs were present. Variation in Rev may be an important mechanism for regulation of virus replication in vivo and may contribute to changes in clinical disease.
Adenovirus Preterminal Protein Binds To The Cad Enzyme At Active Sites Of Viral Dna Replication On The Nuclear Matrix, Peter C. Angeletti, Jeffrey A. Engler
Adenovirus Preterminal Protein Binds To The Cad Enzyme At Active Sites Of Viral Dna Replication On The Nuclear Matrix, Peter C. Angeletti, Jeffrey A. Engler
Nebraska Center for Virology: Faculty Publications
Adenovirus (Ad) replicative complexes form at discrete sites on the nuclear matrix (NM) via an interaction mediated by the precursor of the terminal protein (pTP). The identities of cellular proteins involved in these complexes have remained obscure. We present evidence that pTP binds to a multifunctional pyrimidine biosynthesis enzyme found at replication domains on the NM. Far-Western blotting identified proteins of 150 and 240 kDa that had pTP binding activity. Amino acid sequencing of the 150-kDa band revealed sequence identity to carbamyl phosphate synthetase I (CPS I) and a high degree of homology to the related trifunctional enzyme known as …
Localization Of Human Cytomegalovirus Structural Proteins To The Nuclear Matrix Of Infected Human Fibroblasts, V. Sanchez, Peter C. Angeletti, J. A. Engler, W. J. Britt
Localization Of Human Cytomegalovirus Structural Proteins To The Nuclear Matrix Of Infected Human Fibroblasts, V. Sanchez, Peter C. Angeletti, J. A. Engler, W. J. Britt
Nebraska Center for Virology: Faculty Publications
The intranuclear assembly of herpesvirus subviral particles remains an incompletely understood process. Previous studies have described the nuclear localization of capsid and tegument proteins as well as intranuclear tegumentation of capsid-like particles. The temporally and spatially regulated replication of viral DNA suggests that assembly may also be regulated by compartmentalization of structural proteins. We have investigated the intranuclear location of several structural and nonstructural proteins of human cytomegalovirus (HCMV). Tegument components including pp65 (ppUL83) and ppUL69 and capsid components including the major capsid protein (pUL86) and the small capsid protein (pUL48/49) were retained within the nuclear matrix (NM), whereas the …
Human Immunodeficiency Virus Neurotropism: An Analysis Of Viral Replication And Cytopathicity For Divergent Strains In Monocytes And Microglia, Anuja Ghorpade, Adeline Nukuna, Myhanh Che, Sherryl Haggerty, Yuri Persidsky, Eboni Carter, Leeroy Carhart, Laura Shafer, Howard Gendelman
Human Immunodeficiency Virus Neurotropism: An Analysis Of Viral Replication And Cytopathicity For Divergent Strains In Monocytes And Microglia, Anuja Ghorpade, Adeline Nukuna, Myhanh Che, Sherryl Haggerty, Yuri Persidsky, Eboni Carter, Leeroy Carhart, Laura Shafer, Howard Gendelman
Nebraska Center for Virology: Faculty Publications
Productive replication of human immunodeficiency virus type 1 (HIV-1) in brain macrophages and microglia is a critical component of viral neuropathogenesis. However, how virus-macrophage interactions lead to neurological disease remains incompletely understood. Possibly, a differential ability of virus to replicate in brain tissue macrophages versus macrophages in other tissues underlies HIV-1 neurovirulence. To these ends, we established systems for the isolation and propagation of pure populations of human microglia and then analyzed the viral life cycles of divergent HIV-1 strains in these cells and in cultured monocytes by using identical viral inocula and indicator systems. The HIV-1 isolates included those …
Human Immunodeficiency Virus Neurotropism: An Analysis Of Viral Replication And Cytopathicity For Divergent Strains In Monocytes And Microglia, Anuja Ghorpade, Adeline Nukuna, Myhanh Che, Sheryl Haggerty, Yuri Persidsky, Eboni Carter, Leeroy Carhart, Laura Shafer, Howard Gendelman
Human Immunodeficiency Virus Neurotropism: An Analysis Of Viral Replication And Cytopathicity For Divergent Strains In Monocytes And Microglia, Anuja Ghorpade, Adeline Nukuna, Myhanh Che, Sheryl Haggerty, Yuri Persidsky, Eboni Carter, Leeroy Carhart, Laura Shafer, Howard Gendelman
Nebraska Center for Virology: Faculty Publications
Productive replication of human immunodeficiency virus type 1 (HIV-1) in brain macrophages and microglia is a critical component of viral neuropathogenesis. However, how virus-macrophage interactions lead to neurological disease remains incompletely understood. Possibly, a differential ability of virus to replicate in brain tissue macrophages versus macrophages in other tissues underlies HIV-1 neurovirulence. To these ends, we established systems for the isolation and propagation of pure populations of human microglia and then analyzed the viral life cycles of divergent HIV-1 strains in these cells and in cultured monocytes by using identical viral inocula and indicator systems. The HIV-1 isolates included those …
Role Of The Β-Chemokine Receptors Ccr3 And Ccr5 In Human Immunodeficiency Virus Type 1 Infection Of Monocytes And Microglia, Anuja Ghorpade, Meng Qi Xia, Bradley T. Hyman, Yuri Persidsky, Adeline Nukuna, Paul Bock, Myhanh Che, Janae Limoges, Howard Gendelman, Charles R. Mackay
Role Of The Β-Chemokine Receptors Ccr3 And Ccr5 In Human Immunodeficiency Virus Type 1 Infection Of Monocytes And Microglia, Anuja Ghorpade, Meng Qi Xia, Bradley T. Hyman, Yuri Persidsky, Adeline Nukuna, Paul Bock, Myhanh Che, Janae Limoges, Howard Gendelman, Charles R. Mackay
Nebraska Center for Virology: Faculty Publications
Human immunodeficiency virus type 1 (HIV-1) infection in mononuclear phagocyte lineage cells (monocytes, macrophages, and microglia) is a critical component in the pathogenesis of viral infection. Viral replication in macrophages serves as a reservoir, a site of dissemination, and an instigator for neurological sequelae during HIV-1 disease. Recent studies demonstrated that chemokine receptors are necessary coreceptors for HIV-1 entry which determine viral tropism for different cell types. To investigate the relative contribution of the β -chemokine receptors CCR3 and CCR5 to viral infection of mononuclear phagocytes we utilized a panel of macrophage-tropic HIV-1 strains (from blood and brain tissue) to …
Human Herpesvirus 8 As A Potential Sexually Transmitted Agent In Honduras, C. Sosa, W. Klaskala, B. Chandran, R. Soto, L. Sieczkowski, M. H. Wu, M. Baum, Charles Wood
Human Herpesvirus 8 As A Potential Sexually Transmitted Agent In Honduras, C. Sosa, W. Klaskala, B. Chandran, R. Soto, L. Sieczkowski, M. H. Wu, M. Baum, Charles Wood
Nebraska Center for Virology: Faculty Publications
The seroprevalence of human herpesvirus 8 (HHV-8) was studied in 326 human immunodeficiency virus (HIV)–positive and –negative persons from Honduras; women constituted 77% (n = 251) of the subjects. Sera were tested for lytic HHV-8 antibodies by an IFA, and positive samples were confirmed by a radioimmunoprecipitation assay. Of the 326 persons tested, 58 (17.8%) had HHV- 8 antibodies. Among the HIV-infected women, 22.7% were seropositive; 11.3% of the HIV-negative women were seropositive. HHV-8 seroprevalence was almost four times higher in HIV-positive female commercial sex workers (36%) than in HIV-negative female non–commercial sex workers (9.9%; odds ratio = 3.8, 95% …
Seroprevalence Of Human Herpesvirus 8 Among Zambian Women Of Childbearing Age Without Kaposi’S Sarcoma (Ks) And Mother-Child Pairs With Ks, Jun He, Ganapati Bhat, Chipepo Kankasa, Chifumbe Chintu, Charles Mitchell, Wenjing Duan, Charles Wood
Seroprevalence Of Human Herpesvirus 8 Among Zambian Women Of Childbearing Age Without Kaposi’S Sarcoma (Ks) And Mother-Child Pairs With Ks, Jun He, Ganapati Bhat, Chipepo Kankasa, Chifumbe Chintu, Charles Mitchell, Wenjing Duan, Charles Wood
Nebraska Center for Virology: Faculty Publications
The seroprevalence of human herpesvirus 8 (HHV-8) among a group of Zambian women of reproductive age and among mother-child pairs in which either one of them has Kaposi’s sarcoma (KS) was determined. A cross-sectional group of 378 pregnant women was randomly recruited into the study, and 183 (48.4%) had HHV-8 antibodies. Among the human immunodeficiency virus (HIV)-1–infected women, 51.1% were HHV-8–seropositive, whereas of HIV- 1–negative women, 47.3% were HHV-8–seropositive. In addition, 21 women index patients with KS and 5 young children index patients with KS were studied. All children with KS had mothers who were HHV-8–seropositive, while not all children …