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Articles 1 - 4 of 4
Full-Text Articles in Virology
Near-Complete Genome Sequences Of Vesicular Stomatitis Virus Indiana Laboratory Strains Hr And T1026r1 And Plaque Isolates 22-20 And 22-25, Thomas M. Russell, Evan E. Santo, Lisa Golebiewski, Nathan S. Haseley, Maureen Ferran
Near-Complete Genome Sequences Of Vesicular Stomatitis Virus Indiana Laboratory Strains Hr And T1026r1 And Plaque Isolates 22-20 And 22-25, Thomas M. Russell, Evan E. Santo, Lisa Golebiewski, Nathan S. Haseley, Maureen Ferran
Articles
We report four near-complete genome sequences of vesicular stomatitis virus (VSV) Indiana obtained with Sanger and Illumina next-generation sequencing, namely, laboratory strains HR (heat resistant) and T1026R1 and isolates 22-20 and 22-25. Previously, only the M gene of these viruses had been sequenced, and these sequences were not deposited in GenBank.
Evasion Of Host Innate Immunity By Emerging Viruses: Antagonizing Host Rig-I Pathways, Maureen Ferran, Gary Skuse
Evasion Of Host Innate Immunity By Emerging Viruses: Antagonizing Host Rig-I Pathways, Maureen Ferran, Gary Skuse
Articles
Viruses confront a seemingly dichotomous relationship with their host cells. They must overcome host defenses in order to complete their infectious cycles and generate new viruses yet the host must remain healthy and hospitable for that to take place. Shortly after infection, the RIGI-like receptors (RLRs) within the cytoplasm of the infected cell recognize foreign motifs present in the pathogen. The host responds by activating a signaling pathway that leads to activation of cellular transcription factors, including the NF-κB and interferon regulatory factor 3 (IRF3), that are necessary for induction of the type 1 interferon genes. Many viruses subdue components …
Development And Validation Of A Novel Reporter Assay For Human Papillomavirus Type 16 Late Gene Expression, Beatrice Orru, Ciaran Cunniffe, Fergus Ryan, Stefan Schwartz
Development And Validation Of A Novel Reporter Assay For Human Papillomavirus Type 16 Late Gene Expression, Beatrice Orru, Ciaran Cunniffe, Fergus Ryan, Stefan Schwartz
Articles
To facilitate the investigations of HPV-16 late gene expression HPV-16 reporter plasmids were generated using previously described sub-genomic HPV-16 plasmids, named pBEL and pBELM, that, similar to the full viral genome, produce primarily HPV-16 early mRNAs and very little, if any, late mRNAs in cervical cancer cells. The HPV-16 late L1 gene was replaced by the chloramphenicol acetyltransferase (CAT) reporter gene, or green fluorescent protein (GFP), preceded by the poliovirus internal ribosome entry site (IRES). Results show that the reporter genes mimic the expression of L1 from these plasmids. For example, overexpression of adenovirus E4orf4 protein (E4orf4), polypyrimidine tract binding …
Human Papillomavirus Prevalence And Genotypes In An Opportunistically Screened Irish Female Population, Helen Keegan, Fergus Ryan, Alison Malkin, Mairead Griffin, Helen Lambkin
Human Papillomavirus Prevalence And Genotypes In An Opportunistically Screened Irish Female Population, Helen Keegan, Fergus Ryan, Alison Malkin, Mairead Griffin, Helen Lambkin
Articles
This study aims to evaluate human papillomavirus (HPV) prevalence and predominating genotypes in liquid-based cervical cytology samples from an Irish urban female population. In addition to use of routine cervical cytology testing, women are screened for HPV using the MY09/11 primers for the HPV L1 gene and primers for β-globin amplification in a multiplex format. Overall, 996 women between the ages of 16 and 72 years (average age: 35) are included in the study and HPV prevalence was 19.8%. Cytology results showed that 88.9% were normal, 9% borderline or mild dyskaryosis, 1.1% moderate dyskaryosis and 0.9% severe dyskaryosis. Human papillomavirus …