Open Access. Powered by Scholars. Published by Universities.®
- Discipline
-
- Bacteriology (2)
- Diseases (2)
- Immunology and Infectious Disease (2)
- Medical Sciences (2)
- Medicine and Health Sciences (2)
-
- Organisms (2)
- Pathogenic Microbiology (2)
- Virus Diseases (2)
- Agricultural Science (1)
- Agriculture (1)
- Animal Diseases (1)
- Animal Sciences (1)
- Bacteria (1)
- Biochemistry, Biophysics, and Structural Biology (1)
- Biology (1)
- Biotechnology (1)
- Cell and Developmental Biology (1)
- Dairy Science (1)
- Environmental Microbiology and Microbial Ecology (1)
- Food Microbiology (1)
- Food Science (1)
- Genetics and Genomics (1)
- Immunology of Infectious Disease (1)
- Medical Biochemistry (1)
- Medical Microbiology (1)
- Organismal Biological Physiology (1)
- Parasitic Diseases (1)
- Keyword
-
- 1.6 BIOLOGICAL SCIENCES (2)
- 3. MEDICAL AND HEALTH SCIENCES (2)
- 3.3 HEALTH SCIENCES (2)
- Biochemistry and molecular biology (2)
- Microbiology (2)
-
- Technologies involving identifying the functioning of DNA, (2)
- Virology (2)
- 1.5 EARTH AND RELATED ENVIRONMENTAL SCIENCES (1)
- 3.4 HEALTH BIOTECHNOLOGY (1)
- 4. AGRICULTURAL SCIENCES (1)
- 4.2 ANIMAL AND DAIRY SCIENCE (1)
- 4.4 AGRICULTURAL BIOTECHNOLOGY (1)
- Animal and dairy science (1)
- Biomaterials (1)
- Biopharming (1)
- Cell biology, (1)
- Codon optimization (1)
- Environmental sciences (1)
- Epidemiology (1)
- Expression host (1)
- Gastroenteritis -- Ireland | Virology | Evolution (Biology) | Rotavirus infections -- Ireland | Coronavirus infections | Epidemiology -- Ireland (1)
- Genome (1)
- Infectious diseases (1)
- Johne's disease (1)
- Lactococcus lactis (1)
- MAP antigens (1)
- MAP vaccine (1)
- MMP (1)
- MptD (1)
- Non-dairy (1)
- Publication Type
Articles 1 - 3 of 3
Full-Text Articles in Virology
Enhanced Expression Of Codon Optimized Mycobacterium Avium Subsp. Paratuberculosis Antigens In Lactobacillus Salivarius, Christopher D. Johnston, John P. Bannatine, Rodney Govender, Lorraine Endersen, Daniel Pletzer, Helge Weingart, Aidan Coffey, Jim O'Mahony, Roy D. Sleator
Enhanced Expression Of Codon Optimized Mycobacterium Avium Subsp. Paratuberculosis Antigens In Lactobacillus Salivarius, Christopher D. Johnston, John P. Bannatine, Rodney Govender, Lorraine Endersen, Daniel Pletzer, Helge Weingart, Aidan Coffey, Jim O'Mahony, Roy D. Sleator
Department of Biological Sciences Publications
It is well documented that open reading frames containing high GC content show poor expression in A+T rich hosts. Specifically, G+C-rich codon usage is a limiting factor in heterologous expression of Mycobacterium avium subsp. paratuberculosis (MAP) proteins using Lactobacillus salivarius. However, re-engineering opening reading frames through synonymous substitutions can offset codon bias and greatly enhance MAP protein production in this host. In this report, we demonstrate that codon-usage manipulation of MAP2121c can enhance the heterologous expression of the major membrane protein (MMP), analogous to the form in which it is produced natively by MAP bacilli. When heterologously over-expressed, antigenic determinants …
Phages Of Non-Diary Lactococci: Isolation And Characterization Of Phi L47, A Phage Infecting The Grass Isolate Lactococcus Lactis Ssp Cremoris Dpc6860, Daniel Cavanagh, Caitríona M. Guinane, Horst Neve, Aidan Coffey, R. Paul Ross, Gerald F. Fitzgerald, Olivia Mcauliffe
Phages Of Non-Diary Lactococci: Isolation And Characterization Of Phi L47, A Phage Infecting The Grass Isolate Lactococcus Lactis Ssp Cremoris Dpc6860, Daniel Cavanagh, Caitríona M. Guinane, Horst Neve, Aidan Coffey, R. Paul Ross, Gerald F. Fitzgerald, Olivia Mcauliffe
Department of Biological Sciences Publications
Lactococci isolated from non-dairy sources have been found to possess enhanced metabolic activity when compared to dairy strains. These capabilities may be harnessed through the use of these strains as starter or adjunct cultures to produce more diverse flavor profiles in cheese and other dairy products. To understand the interactions between these organisms and the phages that infect them, a number of phages were isolated against lactococcal strains of non-dairy origin. One such phage, ΦL47, was isolated from a sewage sample using the grass isolate L. lactis ssp. cremoris DPC6860 as a host. Visualization of phage virions by transmission electron …
Gastroenteritis Viruses In Ireland: Epidemiology And Evolution, Lynda Gunn
Gastroenteritis Viruses In Ireland: Epidemiology And Evolution, Lynda Gunn
Theses
Viral gastroenteritis is a common disease of both humans and animals. This thesis focuses on the use of classical virology, coupled with phylogenetics to study gastroenteritis viruses [Bocavirus (BoV), Coronavirus (CoV), and Rotavirus A (RVA)], isolated from selected samples from humans and animals. RVA was associated with outbreaks in elderly care facilities in Northern Ireland, isolates were genotyped and we reported the first detection of G2 lineage lie RVA in Ireland, with other common human RVA genotypes G1P[8], G2P[4] and G9P[8]. Evolutionary dynamics of human RVA VP7 genotypes was examined using codon models of evolution, results show heterogeneous selective pressure …