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Articles 1 - 11 of 11
Full-Text Articles in Virology
Virology: Mutagenizing The Proteolytic Cleavage Site In The Major Capsid Protein In Ssv1, Thejanee Liyanaarachchi
Virology: Mutagenizing The Proteolytic Cleavage Site In The Major Capsid Protein In Ssv1, Thejanee Liyanaarachchi
University Honors Theses
SSV1, spindle-shaped virus 1, is an archaeal virus with a unique shape that belongs to the family of fuselloviruses and infects hyperthermophilic archaea that thrive at 80°C and pH 3. SSV1 has two capsid proteins, VP1 and VP3, that presumably produce the lemon-shaped virion structure characteristic of fuselloviruses. The VP1 amino acid sequence is highly conserved among SSVs. Here, we investigate the importance of the proteolytic cleavage site in position E66 in VP1 by changing its glutamic acid to the other 19 amino acids. We have developed a new protocol for creating SSV1 mutants through HiFi Assembly (NEB) to increase …
A Bioinformatic And Biochemical Analysis Of Cruciviruses, George William Kasun
A Bioinformatic And Biochemical Analysis Of Cruciviruses, George William Kasun
Dissertations and Theses
Cruciviruses are novel ssDNA viruses discovered through metagenomics and direct environmental DNA amplification and cloning. The genomes of cruciviruses suggest that gene transfer between RNA and DNA viruses occurred due to the presence of putative protein-encoding genes that are homologous to both ssRNA and ssDNA viruses. In order to gain a better understanding of this group of viruses both bioinformatic analyses and in vitro biochemical experiments were employed. The results of the bioinformatic analyses show that cruciviruses are a highly diverse group of ssDNA viruses. Their placement within established ssDNA phylogenies is difficult due to heterogeneity in their putative replication-associated …
Bacteriophages Infecting Enterobacter Cloacae To Reduce Bloater Damage In Fermented Cucumbers
Bacteriophages Infecting Enterobacter Cloacae To Reduce Bloater Damage In Fermented Cucumbers
Symposium of Student Scholars
Fermented cucumbers are one of the most important fermented vegetables consumed worldwide. During cucumber fermentations, certain undesirable changes may occur. One of such changes is known as bloater defect (hollow cavities in fermented cucumbers), which is primarily caused by gas-producing bacteria including Enterobacter cloacae. Bloater defect lowers product quality and leads to significant economic loss to the pickle industry, and effective preventative methods are needed. Bacteriophages (phages) are highly host-specific bacterial killers. Use of phages to control unwanted bacteria in foods is a promising approach because phages do not change food properties. This research was to isolate, characterize, and …
Characterizing The Microbiome Of Floral Nectar Of Asclepias Syriaca And Other Asclepias Species, Heather Natterer
Characterizing The Microbiome Of Floral Nectar Of Asclepias Syriaca And Other Asclepias Species, Heather Natterer
Undergraduate Honors Theses
In recent decades, Danaus plexippus (the monarch butterfly) has experienced a drastic decrease in population size due to urbanization, climate change, and habitat loss. The primary habitat and food source of D. plexippus is plant species that are within the genus Asclepias (milkweed). The preservation and propagation of Asclepias spp. is necessary to combat D. plexippus’s population declines. A plant’s reproductive success is dependent upon its ability to attract pollinators, which is linked to the plant’s nectar qualities. We propose that, in turn, these nectar qualities may be influenced by the nectar microbiome. This study aims to better understand the …
Mysteries And Uncertainties In Tracing Cryptic Viral Infections, Melaina Jacoby
Mysteries And Uncertainties In Tracing Cryptic Viral Infections, Melaina Jacoby
Undergraduate Honors Theses
Viruses are the most abundant biological entities on Earth. Viral impacts are evident from the level of individual cells and population all the way up to ecosystems and global elemental cycles. Since bacteriophages (viruses that infect bacteria) were first identified in the early twentieth century, the study of these fascinating entities has shown how viral dynamics within ecosystems can influence microbially-mediated processes at a large scale. Viral infections can impact hosts and host-mediated processes in in multiple ways, one of which is through cryptic infections. This state, in which a bacterium may harbor a cryptic phage infection, is known as …
Antibiotic Resistance Crisis Spurring Phage Therapy Research, Cameron Miguel Perry
Antibiotic Resistance Crisis Spurring Phage Therapy Research, Cameron Miguel Perry
Chancellor’s Honors Program Projects
No abstract provided.
Characterization Of Novel Chlorovirus Glycosyltransferases That Synthesize Atypical Glycans, Eric Noel
Characterization Of Novel Chlorovirus Glycosyltransferases That Synthesize Atypical Glycans, Eric Noel
School of Biological Sciences: Dissertations, Theses, and Student Research
Giant dsDNA chloroviruses encode a diverse repertoire of glycosyltransferases (GTases) and methyltransferases (MTases) that biosynthesize unusual, methylated sugars independent of their host chlorella-like green algae prompting a reexamination of glycobiology systems. Unlike most other viruses, the prototype chlorovirus PBCV-1 encodes most, if not all, of the machinery required to glycosylate its major capsid protein (MCP). The structures of the four N-linked glycans do not resemble any other glycans in the three domains of life. Here, we investigated the potential involvement of chlorovirus- encoded putative GTases and MTases in glycosylation of the viral MCP. First, we aimed to generate site-directed …
Disruption Of Cellular P-Bodies During An Adenovirus Infection, La`Quita Randolph, Kevin Neubrecht, Kasey A. Karen
Disruption Of Cellular P-Bodies During An Adenovirus Infection, La`Quita Randolph, Kevin Neubrecht, Kasey A. Karen
Graduate Research Showcase
Adenovirus has made major contributions in medicine by serving as a model DNA virus to study other viruses, such as human papillomavirus (HPV). Adenoviruses are a diverse family of nonenveloped, double-stranded DNA viruses that are ubiquitous to animals and humans. There are over 67 serotypes of human adenoviruses that can cause a variety of illnesses including, gastroenteritis, conjunctivitis and respiratory infections. Adenovirus can cause these infections by invading host cells and producing an environment that is favorable for viral replication. During the early phases of infection, adenovirus expresses various viral proteins such as E4 11k, which has multiple functions. One …
Optimized Microbial Recombinant Production Of Hiv-1 Anti-Envelope Antibody Fragments With Applications To Single Particle Tracking Of Virus Assembly, Merissa Michelle Bruns
Optimized Microbial Recombinant Production Of Hiv-1 Anti-Envelope Antibody Fragments With Applications To Single Particle Tracking Of Virus Assembly, Merissa Michelle Bruns
Electronic Theses and Dissertations
In my findings, I have established a set series of protocols to recombinantly produce, purify and apply various fluorescent probes in vitro for the fluorescent labeling and study of the human immunodeficiency virus type 1 (HIV-1) envelope (Env) protein during HIV viral assembly. There remains insufficient knowledge about the molecular dynamics and interactions of HIV-1 Env protein with its counterpart, Gag, on the inner host cell surface during assembly of a mature virus particle. There also remains an insufficient amount of data for the understanding and clarification of the mechanism of action of a known host cell HIV-1 restriction factor, …
Ngs Rna-Seq Reveals Bromovirus Encapsidate Native And Recombinant Viral Rnas, As Well As Cellular Rnas From The Host, Nipin Shrestha
Ngs Rna-Seq Reveals Bromovirus Encapsidate Native And Recombinant Viral Rnas, As Well As Cellular Rnas From The Host, Nipin Shrestha
Graduate Research Theses & Dissertations
Brome mosaic virus (BMV), Cowpea chlorotic mottle virus (CCMV), and Broad bean mottle virus (BBMV), the members of the genus Bromovirus, are the experimental model viruses in this dissertation research. Packaging of the RNA genome by the viruses is known to be highly selective and specific for the viral RNA but, not 100% accurate. There is evidence that the viruses specifically or accidentally encapsidate viral RNA variants and host (cellular) RNAs. The scope of this dissertation is to identify different types of RNAs that can be packaged by viruses along with the viral RNAs of parental origin.BMV, CCMV, and BBMV …
Characterization Of Higher Order Chromatin Structures And Chromatin States In Cell Models Of Human Herpesvirus Infection, Michael Mariani
Characterization Of Higher Order Chromatin Structures And Chromatin States In Cell Models Of Human Herpesvirus Infection, Michael Mariani
Graduate College Dissertations and Theses
Human herpesviruses are ubiquitous pathogens worldwide with 90% of the global population infected with one or more Human herpesviruses (HHV’s) by adulthood. All herpesviruses have three unique life cycle stages. Upon resolution of a primary acute stage infection, they can establish a latent stage infection within the host cell nucleus. This stage is characterized primarily by transcriptional quiescence of the viral genome. Specific physiological conditions (e.g., cell stress) can cause the latent virus to enter the reactivation stage, often many years after resolution of the acute infection, in which the virus becomes replicationally active again. HHV’s are known to cause …