Pathogenic Microbiology Commons^™

A Study Of Soil Amoebae Infective To Mice, Pamela Mcdaniel

Masters Theses

On January 1, 1973, 10 soil samples were collected from areas in Coles County, Illinois, in an effort to isolate soil amoebae. All 10 soil samples were cultured on bacto-nutrient agar and colonies of amoebae genera isolated were Acanthamoeba and Hartmannella. Amoebabacteria cultures prepared from soil isolates were injected into each of 10 adult mice which were sacrificed at the end of the nine days and examined for evidence of infection and pathogenicity. Lesions were observed in two mice and amoebae cysts and bacterial colonies were present in nine mice. Amoeba-bacteria cultures were purified using streptomycin antibiotic discs and …