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Canine Testes Thin Sections Culture, Nguyen Khoi Nguyen, Ramanpreet Singh
Canine Testes Thin Sections Culture, Nguyen Khoi Nguyen, Ramanpreet Singh
2023 Symposium
Canine testes thin section culture
Testes tissue culture systems would provide a tool to elucidate spermatogenesis mechanisms, with the aim of genetic preservation of mammals, especially endangered species. Our experiment aims to develop a culture system capable of producing viable mammalian sperm cells in vitro.
Dogs were chosen as the model organism because testes are readily available. Canine testes were obtained from a local veterinary clinic. Thin sections were generated using a commercial electric slicer. They then were cleaned using Dulbecco’s Phosphate-Buffered Saline (DPBS) supplemented with antibiotics then cultured in a modified Tissue Culture Medium 199 (TCM-199). Sections were …
Canine Testes Thin Sections Culture, Nguyen Khoi Nguyen, Ramanpreet Singh
Canine Testes Thin Sections Culture, Nguyen Khoi Nguyen, Ramanpreet Singh
2023 Symposium
Canine testes thin section culture
Testes tissue culture systems would provide a tool to elucidate spermatogenesis mechanisms, with the aim of genetic preservation of mammals, especially endangered species. Our experiment aims to develop a culture system capable of producing viable mammalian sperm cells in vitro.
Dogs were chosen as the model organism because testes are readily available. Canine testes were obtained from a local veterinary clinic. Thin sections were generated using a commercial electric slicer. They then were cleaned using Dulbecco’s Phosphate-Buffered Saline (DPBS) supplemented with antibiotics then cultured in a modified Tissue Culture Medium 199 (TCM-199). Sections were …