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Environmental Microbiology and Microbial Ecology Commons™
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- Biogeochemistry (3)
- Carbon (3)
- Microbiology (3)
- Bacteria (2)
- Cave (2)
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- Climate change (2)
- Earth sciences (2)
- Iron reduction (2)
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Articles 1 - 19 of 19
Full-Text Articles in Environmental Microbiology and Microbial Ecology
Exploring Soil Microbial Dynamics In Southern Appalachian Forests: A Systems Biology Approach To Prescribed Fire Impacts, Saad Abd Ar Rafie
Exploring Soil Microbial Dynamics In Southern Appalachian Forests: A Systems Biology Approach To Prescribed Fire Impacts, Saad Abd Ar Rafie
Doctoral Dissertations
Prescribed fires in Southern Appalachian forests are vital in ecosystem management and wildfire risk mitigation. However, understanding the intricate dynamics between these fires, soil microbial communities, and overall ecosystem health remains challenging. This dissertation addresses this knowledge gap by exploring selected aspects of this complex relationship across three interconnected chapters.
The first chapter investigates the immediate effects of prescribed fires on soil microbial communities. It reveals subtle shifts in porewater chemistry and significant increases in microbial species richness. These findings offer valuable insights into the interplay between soil properties and microbial responses during the early stages following a prescribed fire. …
Investigation Of Shocked Basalts From Vargeão Dome And Vista Alegre: Implications For The Search For Life On Mars, Nikol Posnov
Investigation Of Shocked Basalts From Vargeão Dome And Vista Alegre: Implications For The Search For Life On Mars, Nikol Posnov
Electronic Thesis and Dissertation Repository
Impact craters represent excellent astrobiological targets for planetary exploration missions to Mars. The impact of an asteroid or comet into a crystalline, H2O-bearing target may result in development of a hydrothermal system and increase the habitability of the substrate for the colonization of endolithic microorganisms. Given that Mars’ surface is covered by cratered basaltic rock, this study investigated target rocks and impact breccias from Vargeão Dome and Vista Alegre impact structures that formed in basalt in the Paraná Basin of Brazil.
Utilizing petrography and micro-X-ray diffraction (μXRD), the degree of shock metamorphism in plagioclase was quantitively determined. Measuring …
Physiological Constraints, Mechanisms, And Mineral Transformations Of Iron Reduction In Hyperthermophilic Crenarchaea, Srishti Kashyap
Physiological Constraints, Mechanisms, And Mineral Transformations Of Iron Reduction In Hyperthermophilic Crenarchaea, Srishti Kashyap
Doctoral Dissertations
Dissimilatory iron reduction by hyperthermophilic archaea occurs in many geothermal environments and typically relies on microbe-mineral interactions that transform various iron oxide minerals. However, the kinds of iron oxides that can be used, growth rates, extent of iron reduction, and the mineral transformations that occur due to this metabolism are poorly understood. This dissertation improves our fundamental understanding of the physiological mechanisms and mineral transformations of hyperthermophilic iron reduction using two model crenarchaea, Pyrodictium delaneyi and Pyrobaculum islandicum. Using growth yields and metabolite production rates, we demonstrated that a broad range of Fe(III) (oxyhydr)oxides of variable thermodynamic stability was …
Comparing Capabilities Of Shewanella Oneidensis Mr-1 And The Microbial Community Of Iron Caves To Reduce Fe(Iii), Aaron Douglas Pham
Comparing Capabilities Of Shewanella Oneidensis Mr-1 And The Microbial Community Of Iron Caves To Reduce Fe(Iii), Aaron Douglas Pham
Williams Honors College, Honors Research Projects
Caves are generally formed by the erosion and/or dissolution of rock and its subsequent removal by water. Iron ore caves (IOCs) form despite being hosted by relatively insoluble and weathering-resistant rock. Due to the discovery of a microbial community behind the walls of these caves, it was hypothesized that these bacteria could be responsible for speleogenesis. Iron ore exists in an oxidized (Fe(III)) state, but reduced (Fe(II)) form is soluble. It was further reasoned that the bacteria might be able to reduce Fe(III) through direct metabolic activity, which uses iron as an electron acceptor. Here we show that cave microorganisms …
Carbon Metabolism In Cave Subaerial Biofilms, Victoria E. Frazier
Carbon Metabolism In Cave Subaerial Biofilms, Victoria E. Frazier
Masters Theses
Subaerial biofilms (SABs) grow at the interface between the atmosphere and rock surfaces in terrestrial and subterranean environments around the world. Multi-colored SABs colonizing relatively dry and nutrient-limited cave surfaces are known to contain microbes putatively involved in chemolithoautotrophic processes using inorganic carbon like carbon dioxide (CO2) or methane (CH4). However, the importance of CO2 and CH4 to SAB biomass production has not been quantified, the environmental conditions influencing biomass production and diversity have not been thoroughly evaluated, and stable carbon and nitrogen isotope compositions have yet to be determined from epigenic cave SABs. …
Comparison Of Fe(Iii) Reduction Rates By Iron-Reducing Bacteria Within Sub Muros Samples From Quadrilátero Ferrífero Iron-Ore Caves, Brazil, Summer Ellis
Williams Honors College, Honors Research Projects
Previous research investigating the speleogenesis of iron-ore caves (IOC) in the Quadrilátero Ferrífero, or “Iron Quadrangle,” of Brazil suggests that microbial iron reduction and subsequent dissolution of the surrounding Fe(III) rich rocks is responsible for cave formation. A soft intra-wall substance (sub muros) containing iron-reducing bacteria (FeRB) was discovered underneath the durable crusts of cave walls. The goal of the study was to determine if reduction rates were comparable between sub muros samples, while additionally observing how different electron donors affect microbial iron reduction. To do this, I compared Fe(III) reduction rates between sub muros samples collected from …
Biogeochemical Response To Vegetation And Hydrologic Change In An Alaskan Boreal Fen Ecosystem, Danielle L. Rupp
Biogeochemical Response To Vegetation And Hydrologic Change In An Alaskan Boreal Fen Ecosystem, Danielle L. Rupp
Dissertations, Master's Theses and Master's Reports
Boreal peatlands store approximately one third of the earth’s terrestrial carbon, locked away in currently waterlogged and frozen conditions. Peatlands of boreal and arctic ecosystems are affected increasingly by shifting hydrology caused by climate change. The consequences of these relatively rapid ecosystem changes on carbon cycling between the landscape and the atmosphere could provide an amplifying feedback to climate warming. Alternatively, the advancement of terrestrial vegetation into once waterlogged soils could uptake carbon as a sink. Previous work suggests that fens will become an increasingly dominant landscape feature in the boreal. However, studies investigating fens, their response to hydrologic and …
The Effects Of Seasonal Variations In Chemistry And Hydrology On The Microbial Community And Its Sulfide Oxidation Potential In A Naturally Acidic Maine Stream, Raymond C. Kahler Iii
The Effects Of Seasonal Variations In Chemistry And Hydrology On The Microbial Community And Its Sulfide Oxidation Potential In A Naturally Acidic Maine Stream, Raymond C. Kahler Iii
Electronic Theses and Dissertations
Sulfide minerals oxidize through interaction with water and oxygen, releasing hydrogen ions. The process often occurs naturally near metal sulfide deposits, and can be accelerated through mining. Microorganisms accelerate the rate of sulfide oxidation. Acidified streams typically contain high metal concentrations (e.g. aluminum) and microbes in these systems may develop resistances to metal toxicity. Stream flow can affect sulfide oxidation and microbial community structure. Baseflow can influence stream chemistry from interactions with the surrounding bedrock, while stormflow affects stream chemistry and the local microbial community through dilution and addition of microbes transported by runoff. Microbial community composition is affected by …
Investigation Of Biogeochemical Mechanisms Of Greenhouse Gas Production In The Urban Hudson River Estuary, Brian Alan Brigham
Investigation Of Biogeochemical Mechanisms Of Greenhouse Gas Production In The Urban Hudson River Estuary, Brian Alan Brigham
Dissertations, Theses, and Capstone Projects
Coastal megacities deposit significant amounts of carbon (C), nitrogen (N) and other pollutants into surrounding waters. These inputs, including wastewater and surface water runoff, may affect estuarine and adjacent wetland biogeochemical cycles, microbial production and ultimately greenhouse gas (GHG) efflux. In many megacities pollutant loading is typically greatest after periods of precipitation when the volume of wastewater and storm water runoff exceeds local sanitation capacity, resulting in the discharge of raw sewage into adjacent waters. These combined sewage overflow (CSO) events have received considerable attention primarily due to their potential impact on human health and eutrophication. However, whether these events …
Analysis Of Microbial Diversity In Disturbed Soil, Tyler G. Sanda
Analysis Of Microbial Diversity In Disturbed Soil, Tyler G. Sanda
Williams Honors College, Honors Research Projects
This paper uses the composition and abundance of microbial species to analyze soil recovery in disturbed land. Surface mining disturbs ecological communities throughout the world. As organizations seek to reclaim these disturbed lands, a proper analysis of recovery is needed. In previous studies, recovery of disturbed land was limited to surface examinations, which do not characterize the possible unseen devastating effects of the subsoil. Soil microorganisms are extremely sensitive to environmental changes such as strip mining. It is proposed that these microorganisms may serve as better indicators of recovery post disturbance. Our analysis indicates microbial recovery, however it may not …
Microbial Extracellular Enzymes In Marine Sediments: Methods Development And Potential Activities In The Baltic Sea Deep Biosphere, Jenna Marie Schmidt
Microbial Extracellular Enzymes In Marine Sediments: Methods Development And Potential Activities In The Baltic Sea Deep Biosphere, Jenna Marie Schmidt
Masters Theses
The deep biosphere is defined as the subsurface ecosystem in which little energy is available to microorganisms and microorganisms can live for thousands of years. Heterotrophic microbes survive in the deep biosphere even though organic matter is limited and highly recalcitrant in nature. Measuring microbial extracellular enzyme activity provides a potential means to evaluate the rate at which microorganisms are performing carbon remineralization in the energy limited sediment beneath the seafloor. Extracellular enzymes breakdown organic compounds so that the nutrients can move inside the cell and be used for energy. This study explored the role extracellular enzymes play in the …
Effects Of Epichloë Coenophiala−Tall Fescue Symbiosis On Plant-Microbe-Soil Interactions In A Temperate Pasture, Lindsey C. Slaughter
Effects Of Epichloë Coenophiala−Tall Fescue Symbiosis On Plant-Microbe-Soil Interactions In A Temperate Pasture, Lindsey C. Slaughter
Theses and Dissertations--Plant and Soil Sciences
Plants interact in myriad ways with microorganisms to influence ecosystem processes such as nutrient cycling, which can regulate ecosystem response to global change. One important plant-microbe symbiosis occurs between cool-season grasses and asexual fungal Epichloë endophytes, such as tall fescue (Schedonorus arundinaceus) and Epichloë coenophiala. Because the common toxic strain of the endophyte (CTE) harms grazing livestock, non-livestock toxic endophyte (NTE) strains have been developed and are increasingly deployed in pastures. Little is known about how these symbioses impact other plant-microbe interactions and microbe-mediated soil processes in grassland ecosystems. I conducted three studies to determine how E. …
Bacterial Diversity And Function Within An Epigenic Cave System And Implications For Other Limestone Cave Systems, Kathleen Merritt Brannen-Donnelly
Bacterial Diversity And Function Within An Epigenic Cave System And Implications For Other Limestone Cave Systems, Kathleen Merritt Brannen-Donnelly
Doctoral Dissertations
There are approximately 48,000 known cave systems in the United States of America, with caves formed in carbonate karst terrains being the most common. Epigenic systems develop from the downward flow of meteoric water through carbonate bedrock and the solutional enlargement of interconnected subsurface conduits. Despite carbonate karst aquifers being globally extensive and important drinking water sources, microbial diversity and function are poorly understood compared to other Earth environments. After several decades of research, studies have shown that microorganisms in caves affect water quality, rates of carbonate dissolution and precipitation, and ecosystem nutrition through organic matter cycling. However, limited prior …
Rare Occurrences Of Free-Living Bacteria Belonging To Sedimenticola From Subtidal Seagrass Beds Associated With The Lucinid Clam, Stewartia Floridana, Aaron M. Goemann
Rare Occurrences Of Free-Living Bacteria Belonging To Sedimenticola From Subtidal Seagrass Beds Associated With The Lucinid Clam, Stewartia Floridana, Aaron M. Goemann
Masters Theses
Lucinid clams and their sulfur-oxidizing endosymbionts comprise two compartments of a three-stage, biogeochemical relationship among the clams, seagrasses, and microbial communities in marine sediments. A population of the lucinid clam, Stewartia floridana, was sampled from a subtidal seagrass bed at Bokeelia Island Seaport in Florida to test the hypotheses: (1) S. floridana, like other lucinids, are more abundant in seagrass beds than bare sediments; (2) S. floridana gill microbiomes are dominated by one bacterial operational taxonomic unit (OTU) at a sequence similarity threshold level of 97% (a common cutoff for species level taxonomy) from 16S rRNA genes; …
Methane Emissions From Direct-Seeded, Delayed-Flood Rice Grown On A Clay Soil, Alden Daniel Smartt
Methane Emissions From Direct-Seeded, Delayed-Flood Rice Grown On A Clay Soil, Alden Daniel Smartt
Graduate Theses and Dissertations
Due to the production of methane (CH4) under flooded-soil conditions, rice (Oryza sativa L.) cultivation is a major contributor to agricultural CH4 emissions. Studies examining CH4 emissions from rice have only recently been initiated in Arkansas and no data have been collected from rice produced on clay soils in Arkansas. Therefore, research was conducted in 2012 and 2013 at the Northeast Research and Extension Center in Keiser, Arkansas to examine the factors affecting CH4 emissions from rice produced on a Sharkey clay (very-fine, smectitic, thermic Chromic Epiaquerts). The objectives of this study were to determine: 1) the effect of vegetation …
Microbe-Mineral Relationships And Biogenic Mineral Transformations In Actively Venting Deep-Sea Hydrothermal Sulfide Chimneys, Tzihsuan J. Lin
Microbe-Mineral Relationships And Biogenic Mineral Transformations In Actively Venting Deep-Sea Hydrothermal Sulfide Chimneys, Tzihsuan J. Lin
Doctoral Dissertations
This dissertation uses a combination of microbiology, mineralogy, and geochemistry to understand dissimilatory iron reduction in hyperthermophilic archaea and the role and potential impact of these and other vent microorganisms within active deep-sea hydrothermal vent chimneys. The central objective of the dissertation is to determine if mineral composition and chimney type are among the primary determinants of microbial community composition and hyperthermophilic, dissimilatory iron reducer growth, in addition to other environmental factors such as nutrient availability, temperature, pH, and chlorinity. This is done using samples and organisms collected from the Endeavour Segment of the Juan de Fuca Ridge in the …
The Influence Of Hydrogeomorphology, Soil Redox Conditions, And Salinity On The Spatial Zoning Of Saltgrass, Salt Rush, And Cattails In Scotts Creek Marsh, Swanton Pacific Ranch, Ca, Mark D. Gormley
Master's Theses
Scotts Creek Marsh (SCM) is a small coastal wetland ecosystem in Davenport, CA. The vegetation of SCM is dominated by three halophytic zones comprised of saltgrass, salt rush, cattails. The objectives of the study were (i) to investigate the variables that influence the zoning of the three dominant halophyte communities in SCM and (ii) to the test the effectiveness of Indicator of Reduction in Soil (IRIS) tubes to indicate the reduction of S. The study examined the following parameters from April 6 to July 21, 2013: (i) the HGM of Scotts Creek Marsh, (ii) soil oxidation and reduction (redox) conditions, …
Genetic Analysis Of Bacterial Gene Variations In Sulfidic Springs And The Influence On Geochemistry, Brendan Joseph Headd
Genetic Analysis Of Bacterial Gene Variations In Sulfidic Springs And The Influence On Geochemistry, Brendan Joseph Headd
Doctoral Dissertations
Culture-independent methods have revolutionized environmental microbiology and geomicrobiology studies and next-generation sequencing and metagenomics techniques continue to reveal the vast genetic diversity of microorganisms. But, these approaches provide comparatively little quantitative information about the roles that naturally occurring microbial gene variations play in local biogeochemical cycling. The goal of this study was to identify how the physical distribution and genetic diversity of microbial genes within a habitat impact environmental geochemistry by examining the biogeography of 16S rRNA genes and bacterial sulfur oxidation (Sox) genes in terrestrial sulfidic springs. 16S rRNA gene pyrosequences were obtained from microbial mats inhabiting eight sulfidic …
Litter Conditioning Is Differentially Affected By Leaf Species, Phosphorus Enrichment, And Light Availability, Erin E. Scott
Litter Conditioning Is Differentially Affected By Leaf Species, Phosphorus Enrichment, And Light Availability, Erin E. Scott
Graduate Theses and Dissertations
Anthropogenic enrichment of nitrogen and phosphorus is one of the most pervasive and detrimental threats to aquatic ecosystems worldwide. In streams that rely on allochthonous basal food resources, such as leaves, nutrient pollution can result in altered food quality and decreased carbon (C) standing stocks. However, the magnitude and mechanisms of this change in quality are poorly understood. Laboratory microcosm studies were conducted to 1) quantify the response of litter C:P to a gradient of phosphorus (P) enrichment (0, 0.05, and 0.5 mg SRP/L) across leaf species with variable levels of degradability (sugar maple and oak), and 2) quantify the …