Microbiology Commons^™

Georgeoral Immunization Of Rhesus Macaques With Adenoviral Hiv Vaccines Using Enteric-Coated Capsules, George T. Mercier, Pramod N. Nehete, Marco F. Passeri, Bharti N. Nehete, Eric A. Weaver, Nancy Smyth Templeton, Kimberly Schluns, Stephanie S. Buchl, K. Jagannadha Sastry, Michael A. Barry

Nebraska Center for Virology: Faculty Publications

Targeted delivery of vaccine candidates to the gastrointestinal (GI) tract holds potential for mucosal immunization, particularly against mucosal pathogens like the human immunodeficiency virus (HIV). Among the different strategies for achieving targeted release in the GI tract, namely the small intestine, pH sensitive enteric coating polymers have been shown to protect solid oral dosage forms from the harsh digestive environment of the stomach and dissolve relatively rapidly in the small intestine by taking advantage of the luminal pH gradient. We developed an enteric polymethacrylate formulation for coating hydroxy-propyl-methyl-cellulose (HPMC) capsules containing lyophilized Adenoviral type 5 (Ad5) vectors expressing HIV-1 gag …

A Protein Encoded By The Bovine Herpesvirus 1 Latency-Related Gene Interacts With Specific Cellular Regulatory Proteins, Including Ccaat Enhancer Binding Protein Alpha, Florencia Meyer, Sandra Perez, Vicki Geiser, Mark Sintek, Melissa Inman, Clinton J. Jones

Nebraska Center for Virology: Faculty Publications

Following acute infection, bovine herpesvirus 1 establishes latency in sensory neurons of trigeminal ganglia (TG). Reactivation from latency occurs periodically, resulting in the shedding of infectious virus. The latency-related (LR) RNA is abundantly expressed in TG of latently infected calves, and the expression of LR proteins is necessary for dexamethasone-induced reactivation from latency. Previously published studies also identified an alternatively spliced LR transcript which is abundantly expressed in TG at 7 days after infection and has the potential to encode a novel LR fusion protein. Seven days after infection is when extensive viral gene expression is extinguished in TG and …

Genetic Microheterogeneity And Phenotypic Variation Of Helicobacter Pylori Arginase In Clinical Isolates, Justin Hovey, Emily Watson, Melanie Langford, Ellen Hildebrandt, Sangeetha Bathala, Jeffrey Bolland, Domenico Spadafora, George Mendz, David Mcgee

School of Biological Sciences: Faculty Publications

Background: Clinical isolates of the gastric pathogen Helicobacter pylori display a high level of genetic macro- and microheterogeneity, featuring a panmictic, rather than clonal structure. The ability of H. pylori to survive the stomach acid is due, in part, to the arginase-urease enzyme system. Arginase (RocF) hydrolyzes L-arginine to L-ornithine and urea, and urease hydrolyzes urea to carbon dioxide and ammonium, which can neutralize acid.

Results: The degree of variation in arginase was explored at the DNA sequence, enzyme activity and protein expression levels. To this end, arginase activity was measured from 73 minimally-passaged clinical isolates and six laboratory-adapted strains …

Evolutionary Endocrinology: The Developing Synthesis Between Endocrinology And Evolutionary Genetics, Anthony J. Zera, Lawrence G. Harshman, Tony D. Williams

Anthony Zera Publications

A productive synthesis of endocrinology and evolutionary genetics has occurred during the past two decades, resulting in the first direct documentation of genetic variation and correlation for endocrine regulators in nondomesticated animals. In a number of insect genetic polymorphisms (dispersal polymorphism in crickets, butterfly wing-pattern polymorphism), blood levels of ecdysteroids and juvenile hormone covary with morphology, development, and life history. Genetic variation in insulin signaling may underlie life history trade-offs in Drosophila. Vertebrate studies identified variation in brain neurohormones, bone-regulating hormones, and hormone receptor gene sequences that underlie ecologically important genetic polymorphisms. Most work to date has focused on genetically …

Establishment And Maintenance Of An Asm Student Chapter, Amy Cheng Vollmer

Biology Faculty Works

No abstract provided.

Good Things Come In Small Packages: Tiny Plankton Producing Oxygen Near Dead Zone, Abbie Basile

Libraries Faculty & Staff Publications

(First paragraph) For many, the words “Lake Erie” bring to mind large things: big sport fish, sprawling waterfront homes, and an enormous body of water bordering four states and two countries. Similarly, much of the Lake Erie research of which the public is aware deals with larger biological organisms—larger algae and zooplankton, the small fish that eat the zooplankton, and the large fish that feed on those smaller fish. Over the years, the smaller lake life have been somewhat overlooked.

Assessment Of Key Parameters To Achieve Bacteria Transformability In E. Coli And A. Tumefaciens, Zach Regelin

Honors Program Theses

ln this project, growth and transformability of E. coli and A. tumefaciens were investigated. The growth of the bacterial species was monitored under different key parameters including: the strain of the bacteria, the inoculum source (a fresh plate or frozen glycerol stock), and the media type. Growth was monitored by measuring the optical density (OD). Using E. coli strains Dh5a and CdB\ it was determined that the strain, the media type, and the amount of inoculum had a great effect on the growth but the source of the inoculum had little effect. For A. tumefaciens, the strain and the media …

Microbiological Enhancement Of Phosphorus Sequestration In Iron-Enriched Sediments, Lindsay Marie Sandersfeld

Honors Program Theses

An overabundance of phosphorus, primarily due to agricultural run off, is the source of increases in unwanted algae in Iowa's surface waters. Oxidized iron found in mine tailings will sequester phosphorus in sediments and prevent it from entering surface waters. Therefore, adding mine tailings to wetlands is a cheap and easy way to eliminate phosphorus pollution. This phosphorus removal process is enhanced by a microbial component that biochemically solubilizes iron tailings which allows them to efficiently react with free phosphorus in the water. The purpose of this project was to characterize the microbial component in this process. Ferric citrate medium …

Human Papillomavirus Prevalence And Genotypes In An Opportunistically Screened Irish Female Population, Helen Keegan, Fergus Ryan, Alison Malkin, Mairead Griffin, Helen Lambkin

Articles

This study aims to evaluate human papillomavirus (HPV) prevalence and predominating genotypes in liquid-based cervical cytology samples from an Irish urban female population. In addition to use of routine cervical cytology testing, women are screened for HPV using the MY09/11 primers for the HPV L1 gene and primers for β-globin amplification in a multiplex format. Overall, 996 women between the ages of 16 and 72 years (average age: 35) are included in the study and HPV prevalence was 19.8%. Cytology results showed that 88.9% were normal, 9% borderline or mild dyskaryosis, 1.1% moderate dyskaryosis and 0.9% severe dyskaryosis. Human papillomavirus …

Characterization Of Mycobacterium Tuberculosis Central Asian Strain 1 Using Mycobacterial Interspersed Repetitive Unit Genotyping, Asho Ali, Zahra Hasan, Mahnaz Tanveer, Amna Siddiqui, Solomon Ghebremichael, Gunilla Kallenius, Rumina Hasan

Department of Pathology and Laboratory Medicine

Background:
The Central Asian Strain1 (CAS1) genogroup of Mycobacterium tuberculosis (MTB) is the most prevalent in Pakistan, India and Bangladesh. Mycobacterial interspersed repetitive units variable number tandem repeat (MIRU-VNTR) typing is a reliable and reproducible method for differentiation of MTB isolates. However, information of its utility in determining the diversity of CAS1 strain is limited. We performed standard 12 loci based MIRU-VNTR typing on previously spoligotyped CAS1 strains and 'unique' strains in order to evaluate its discriminatory power for these isolates.
Methods:
Twelve loci based MIRU- VNTR typing was used to type178 CAS1 and 189 'unique' MTB strains. The discriminatory …

Comparative Predictive Value Of Three Prognostic Markers--S-Phase Fraction, Pcna And Mitotic Count On Axillary Lymph Node Metastasis In Carcinoma Breast, Shahid Pervez, Muhammad Nadeem Khan, Muhammad Israr Nasir

Department of Pathology and Laboratory Medicine

Background: Axillary lymph node metastasis is the single most important prognostic factor in carcinoma of the breast. Therefore, prognostic markers that may reliably predict probability of lymph node (LN) metastases are of great value. This study was conducted to compare the predictive value of two novel prognostic / proliferative markers i.e. S-phase fraction (SPF) and proliferating cell nuclear antigen (PCNA) in parallel with mitotic index.

Methods: Data of consecutive cases of infiltrating ductal carcinoma (IDC) breast diagnosed from July 2003 to July 2004 at the section of the Histopathology, The Aga Khan University Hospital, Karachi, were reviewed. A total of …