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1996

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Articles 31 - 48 of 48

Full-Text Articles in Microbiology

Effects Of Sunlight On Bacteriophage Viability And Structure, Ke Wommack, Rt Hill, Ta Muller, Rr Colwell Apr 1996

Effects Of Sunlight On Bacteriophage Viability And Structure, Ke Wommack, Rt Hill, Ta Muller, Rr Colwell

VIMS Articles

Current estimates of viral abundance in natural waters rely on direct counts of virus-like particles (VLPs), using either transmission or epifluorescence microscopy. Direct counts of VLPs, while useful in studies of viral ecology, do not indicate whether the observed VLPs are capable of infection and/or replication. Rapid decay in bacteriophage viability under environmental conditions has been observed. However, it has not been firmly established whether there is a corresponding degradation of the virus particles, To address this question, viable and direct counts were carried out employing two Chesapeake Bay bacteriophages in experimental microcosms incubated for 56 h at two depths …

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Examination Of The Endosomal And Lysosomal Pathways In Dictyostelium Discoideum Myosin I Mutants, Lesly A. Temesvari, John M. Bush, Michelle D. Peterson, Kristine D. Novak, Margaret A. Titus, James A. Cardelli Mar 1996

Examination Of The Endosomal And Lysosomal Pathways In Dictyostelium Discoideum Myosin I Mutants, Lesly A. Temesvari, John M. Bush, Michelle D. Peterson, Kristine D. Novak, Margaret A. Titus, James A. Cardelli

Publications

The role of myosin Is in endosomal trafficking and the lysosomal system was investigated in a Dictyostelium discoideum myosin I double mutant myoB-/C-, that has been previously shown to exhibit defects in fluid-phase endocytosis during growth in suspension culture (Novak et al., 1995). Various properties of the endosomal pathway in the myoB-/C- double mutant as well as in the myoB- and myoC- single mutants, including intravesicular pH, and intracellular retention time and exocytosis of a fluid phase marker, were found to be indistinguishable from wild-type parental cells. The intimate connection between the contractile vacuole complex and the endocytic pathway in …

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Artificial Selection On Jhe Activity In Gryllus Assimilis: Nature Of Activity Differences Between Lines And Effect On Jh Binding And Metabolism, Anthony J. Zera, Jeffrey Sall, Robert Schwartz Feb 1996

Artificial Selection On Jhe Activity In Gryllus Assimilis: Nature Of Activity Differences Between Lines And Effect On Jh Binding And Metabolism, Anthony J. Zera, Jeffrey Sall, Robert Schwartz

Anthony Zera Publications

Genetic lines of the cricket Gryllus assimilis selected for elevated vs. decreased activity of hemolymph juvenile hormone esterase (JHE) exhibited a 6.8-fold difference in enzyme activity by the seventh generation of selection. This documents that hemolymph JHE activity has the capability for rapid evolutionary change. This is the only insect endocrine trait for which such data are currently available. The difference in hemolymph JHE activity between the lines was due, to an equivalent degree, to variation in whole-cricket enzyme activity and allocation of JHE activity to the hemolymph compartment. No differences in kinetic or thermostability characteristics were observed between JHEs …

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Infection Of Primary Human Fetal Astrocytes By Human Herpesvirus 6, Jun He, Micheline Mccarthy, Yi Zhou, Bala Chandran, Charles Wood Feb 1996

Infection Of Primary Human Fetal Astrocytes By Human Herpesvirus 6, Jun He, Micheline Mccarthy, Yi Zhou, Bala Chandran, Charles Wood

Nebraska Center for Virology: Faculty Publications

Human herpesvirus 6 (HHV-6) is a lymphotropic betaherpesvirus which productively infects human CD4+ T cells and monocytes. HHV-6 is the etiologic agent for exanthem subitum (roseola), and it is well-known that central nervous system complications occur frequently during the course of HHV-6-associated disease. In addition, HHV-6 has been associated with encephalitis or encephalopathy. However, very little is known about its tropism for neural cells. There are reports that HHV-6 may infect some glial cell lines, but whether it can infect any primary neural cells is not known. Our studies show that both HHV-6A (GS) and HHV-6B (Z-29) can infect …

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V, D And J Gene Rearrangements On The Unexpressed Igh Allele In Rabbit B Cells, Chainarong Tunyaplin Jan 1996

V, D And J Gene Rearrangements On The Unexpressed Igh Allele In Rabbit B Cells, Chainarong Tunyaplin

Dissertations

No abstract provided.

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Nonsense Codon-Mediated Degradation Of Immunoglobulin Heavy-Chain [Mu] Messenger Rna In B Cells, Tianhong Li Jan 1996

Nonsense Codon-Mediated Degradation Of Immunoglobulin Heavy-Chain [Mu] Messenger Rna In B Cells, Tianhong Li

Dissertations

No abstract provided.

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So You Want To Be A Soil Microbiologist?, Mark S. Coyne Jan 1996

So You Want To Be A Soil Microbiologist?, Mark S. Coyne

Plant and Soil Sciences Faculty Book Gallery

A tongue-in-cheek look at soil microbiology.

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Herpesvirus-Like Dna In Aids Kaposi’S Sarcoma In Argentina [Letter To The Editor], Carlos E. Sosa, Jorge A. Benetucci, Laura M. Sieczkowski, Anibal Vazquez, Xue-Lan Wen, Marianna Baum, Winslow Klaskala, William J. Harrington Jr., Charles Wood Jan 1996

Herpesvirus-Like Dna In Aids Kaposi’S Sarcoma In Argentina [Letter To The Editor], Carlos E. Sosa, Jorge A. Benetucci, Laura M. Sieczkowski, Anibal Vazquez, Xue-Lan Wen, Marianna Baum, Winslow Klaskala, William J. Harrington Jr., Charles Wood

Nebraska Center for Virology: Faculty Publications

First paragraph: Recently, Chang et al., using a new molecular biology technique termed Representational Difference Analysis, found herpesvirus-like DNA (KSHV) in AIDS patients with Kaposi’s sarcoma (KS). The presence of KSHV DNA sequences suggests that a new human herpesvirus may be associated with KS. The 5′ end of the 1853-bp flanking region of KSHV (nucleotides 1 to 607) was found to have 66 and 67% homologies to the corresponding regions of the major capsid protein gene of Herpesvirus Saimiri (ORF25) and Epstein Barr virus (BcLF1), respectively, both members of the gammaherpesvirus family. This finding is an important breakthrough, because a …

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The Lipooligosaccharides Of Haemophilus Ducreyi Are Highly Sialylated, William Melaugh, A A. Campagnari, B W. Gibson Jan 1996

The Lipooligosaccharides Of Haemophilus Ducreyi Are Highly Sialylated, William Melaugh, A A. Campagnari, B W. Gibson

Chemistry Faculty Publications

The major lipooligosaccharides of the sexually transmitted pathogen Haemophilus ducreyi 35000 have been previously found to terminate in N-acetyllactosamine and sialyl-N-acetyllactosamine, Neu5Ac alpha 2-->3Gal beta 1-->4GlcNAc (W. Melaugh, N. J. Phillips, A. A. Campagnari, M. V. Tullius, and B. W. Gibson, Biochemistry 33: 13070-13078, 1994). In this study, mass spectrometry and composition analyses have shown that the lipooligosaccharides from three other H. ducreyi strains also contain N-acetyllactosamine and are highly sialylated (approximately 30 to 50%), although one African strain was found to contain neither of these structural features.

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Cyanonews (Vol. 12, No. 2, July 1996), Jeff Elhai Jan 1996

Cyanonews (Vol. 12, No. 2, July 1996), Jeff Elhai

CyanoNews

CyanoNews was a newsletter that served the cyanobacteriological community from 1985 to 2003, with content provided by readers (sort of a blog before there were blogs). The newsletter reported new findings from the lab, summaries of recent meetings (often provided by graduate students and post-docs entering the field), positions sought or available, life transitions, a compendium of recent cyanobacteria-related articles, and other items of interest to those who study cyanobacteria.

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Cyanonews (Vol. 12, No. 1, January 1996), Jeff Elhai Jan 1996

Cyanonews (Vol. 12, No. 1, January 1996), Jeff Elhai

CyanoNews

CyanoNews was a newsletter that served the cyanobacteriological community from 1985 to 2003, with content provided by readers (sort of a blog before there were blogs). The newsletter reported new findings from the lab, summaries of recent meetings (often provided by graduate students and post-docs entering the field), positions sought or available, life transitions, a compendium of recent cyanobacteria-related articles, and other items of interest to those who study cyanobacteria.

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Bacillus Vallismortis Sp. Nov., A Close Relative Of Bacillus Subtilis, Isolated From Soil In Death Valley, California, Michael Roberts, Lawrence Nakamura, Frederick Cohan Jan 1996

Bacillus Vallismortis Sp. Nov., A Close Relative Of Bacillus Subtilis, Isolated From Soil In Death Valley, California, Michael Roberts, Lawrence Nakamura, Frederick Cohan

Frederick M. Cohan

No abstract provided.

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Reca-Like Genes From Three Archaean Species With Putative Protein Products Similar To Rad51 And Dmcl Proteins Of The Yeast Saccharomyces Cerevisiae, Steven Sandler, Leslie H. Satin, Hardeep Sarma, Alvin J. Clark Jan 1996

Reca-Like Genes From Three Archaean Species With Putative Protein Products Similar To Rad51 And Dmcl Proteins Of The Yeast Saccharomyces Cerevisiae, Steven Sandler, Leslie H. Satin, Hardeep Sarma, Alvin J. Clark

Steven Sandler

The process of homologous recombination has been documented in bacterial and eucaryotic organisms. The Escherichia coliRecA and Saccharomyces cerevisiae Rad51 proteins are the archetypal members of two related families of proteins that play a central role in this process. Using the PCR process primed by degenerate oligonucleotides designed to encode regions of the proteins showing the greatest degree of identity, we examined DNA from three organisms of a third phylogenetically divergent group, Archaea, for sequences encoding proteins similar to RecA and Rad51. The archaeans examined were a hyperthermophilic acidophile, Sulfolobus sofataricus (Sso); a halophile, Haloferax volcanii (Hvo); and a hyperthermophilic …

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Physical Linkage Of The Vibrio Cholerae Mannose-Sensitive Hemagglutinin Secretory And Structural Subunit Gene Loci: Identification Of The Mshg Coding Sequence., Jane W. Marsh, Daxi Sun, Ronald K. Taylor Jan 1996

Physical Linkage Of The Vibrio Cholerae Mannose-Sensitive Hemagglutinin Secretory And Structural Subunit Gene Loci: Identification Of The Mshg Coding Sequence., Jane W. Marsh, Daxi Sun, Ronald K. Taylor

Dartmouth Scholarship

Vibrio cholerae O1 expresses a variety of cell surface factors which mediate bacterial adherence and colonization at the intestinal epithelium. The mannose-sensitive hemagglutinin (MSHA), a type IV pilus, is a potential attachment factor of the V. cholerae El Tor biotype. We describe a TnphoA mutant that is defective in its ability to hemagglutinate mouse erythrocytes. The TnphoA insertion maps to a recently identified genetic locus that encodes products that are predicted to be essential for assembly and export of the MSHA pilus. Insertional disruption at this locus in a mshA-phoA reporter strain provides evidence for a role of this locus …

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Communities Of Cloacal Bacteria In Tree Swallow Families, Michael P. Lombardo, Patrick A. Thorpe, R. Cichewicz, M. Henshaw, C. Millard, C. Steen, T. K. Zeller Jan 1996

Communities Of Cloacal Bacteria In Tree Swallow Families, Michael P. Lombardo, Patrick A. Thorpe, R. Cichewicz, M. Henshaw, C. Millard, C. Steen, T. K. Zeller

Peer Reviewed Publications

Our aim in this study was to survey the communities of bacteria found in the cloacae of adult and nestling Tree Swallows (Tachycineta bicolor), determine if there were familial patterns of prevalence, and determine if there were relationships between bacteria loads and nestling size when 12 days old and fledging success.

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Amino Acid Substitutions In Membrane-Spanning Domains Of Hol1, A Member Of The Major Facilitator Superfamily Of Transporters, Confer Nonselective Cation Uptake In Saccharomyces Cerevisiae, Matthew B. Wright, Elizabeth A. Howell, Richard F. Gaber Jan 1996

Amino Acid Substitutions In Membrane-Spanning Domains Of Hol1, A Member Of The Major Facilitator Superfamily Of Transporters, Confer Nonselective Cation Uptake In Saccharomyces Cerevisiae, Matthew B. Wright, Elizabeth A. Howell, Richard F. Gaber

University Faculty Publications and Creative Works

Let A(formula presented) -dimensional compactum and (formula presented)Mn a map into a piecewise linear (formula presented). The main result of this paper asserts that if X is locally (formula presented)-connected and/is (formula presented)-connected, th

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Growth Of Bacterioplankton And Consumption Of Dissolved Organic Carbon In The Sargasso Sea, C. A. Carlson, H. W. Ducklow Jan 1996

Growth Of Bacterioplankton And Consumption Of Dissolved Organic Carbon In The Sargasso Sea, C. A. Carlson, H. W. Ducklow

VIMS Articles

Lability of the bulk dissolved organic carbon (DOG) pool and the amount available to bacterioplankton on short time scales (hours to days) were examined in oligotrophic Sargasso Sea water (near Bermuda). We examined bacterial growth and DOC utilization using seawater culture methodology in combination with measurements of bacterial abundance, cell volume, and DOC. Bulk DOC concentrations were determined by high temperature combustion (HTC) analysis, which proved to be a sensitive method for detecting small changes in natural concentration of DOG. Measurable bacterial growth and DOC utilization only occurred in unamended cultures when initial DDC concentrations were greater than observed in …

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Homology Among Nearly All Plasmids Infecting Three Bacillus Species, Piotr Zawadzki, Margaret A. Riley, Frederick M. Cohan Dec 1995

Homology Among Nearly All Plasmids Infecting Three Bacillus Species, Piotr Zawadzki, Margaret A. Riley, Frederick M. Cohan

Frederick M. Cohan

No abstract provided.

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