Microbiology Commons^™

Use Of Pyocin To Select A Haemophilus Ducreyi Variant Defective In Lipooligosaccharide Biosynthesis, A A. Campagnari, R Karalus, M A. Apicella, William Melaugh, A J. Lesse, B W. Gibson

Chemistry Faculty Publications

Haemophilus ducreyi, a cause of genital ulcer disease in developing countries, appears to facilitate the heterosexual transmission of the human immunodeficiency virus in Africa. Despite an increase in studies of this gram-negative human pathogen, little is known about the pathogenesis of chancroid. Our studies have shown that the lipooligosaccharides (LOS) of H. ducreyi may play an important role in ulcer formation. Monoclonal antibody and mass spectrometric analyses identified a terminal trisaccharide present on H. ducreyi LOS that is immunochemically similar to human paragloboside. This epitope is present on the LOS of Neisseria gonorrhoeae, and it may be the site of …

Cyanonews (Vol. 10, No. 2, July 1994), Jeff Elhai

CyanoNews

CyanoNews was a newsletter that served the cyanobacteriological community from 1985 to 2003, with content provided by readers (sort of a blog before there were blogs). The newsletter reported new findings from the lab, summaries of recent meetings (often provided by graduate students and post-docs entering the field), positions sought or available, life transitions, a compendium of recent cyanobacteria-related articles, and other items of interest to those who study cyanobacteria.

Cyanonews (Vol. 10, No. 1, February 1994), Jeff Elhai

CyanoNews

CyanoNews was a newsletter that served the cyanobacteriological community from 1985 to 2003, with content provided by readers (sort of a blog before there were blogs). The newsletter reported new findings from the lab, summaries of recent meetings (often provided by graduate students and post-docs entering the field), positions sought or available, life transitions, a compendium of recent cyanobacteria-related articles, and other items of interest to those who study cyanobacteria.

60 Hz Magnetic Field Exposure Inhibits Protein Kinase C Dependent Induction Of Neuropeptide Y Mrna In Pc-12 Cells, William James Thomas

Theses Digitization Project

The effects of MF exposure on the chemically induced production of NPY mRNA in the rat pheochromocytoma, PC-12 cell line.

Virus Diseases Of Subterranean Clover Pastures And Their Management, David Ferris, Roger Jones

Journal of the Department of Agriculture, Western Australia, Series 4

In Western Australia and southern Australia generally, subterranean clover mottle (SCMV) and bean yellow mosaic (BYMV) are the most damaging virus diseases of subterranean clover pastures.

Although infected plants cannot be cured of virus infection, pastures can be managed to reduce both virus spread and persistence from year to year. Management options depend on which virus is present and include reseeding with resistant varieties; changing the pasture composition so that non-host species predominate; altering grazing practices to limit spread; and application of pesticide sprays to kill aphids.

Miscellaneous Publication No 33/93 - Virulent Footrot A Farmer's Guide To Eradication, Department Of Agriculture, Western Australia

Animal production and livestock published reports

The first thing to understand about virulent footrot is that it can occur on any farm in the medium to high rainfall zone. Persons who regularly buy and sell sheep have a greater risk, but even closed breeding flock properties are at risk from stray sheep.

People often associate footrot with properties managed by inefficient farmers. This is a false belief. Many good farmers have found the disease in their sheep, including high profile studs.

Footrot can strike anywhere and often without warning.

The important thing to remember is that once it is found, getting to know and understand the …

Characterization Of Fibroblasts With A Unique Defect In Processing Antigens With Disulfide Bonds, Brian J. Merkel

Theses and Dissertations

A Chinese hamster ovary (CHO) fibroblast, transfected with murine major histocompatibility complex (MHC) class II genes, inefficiently simulated CD4+ Th cells specific for ovalbumin (OVA), hen egg lysozyme (HEL), and pork insulin which contains disulfide bonds. However, the fibroblasts elicited a T cell response to λ-repressor, which lacks disulfide bonds, and efficiently presented synthetic peptides. A somatic cell hybrid WALC, generated by fusing the hamster fibroblast with a murine L cell fibroblast, very efficiently processed OVA and HEL, suggesting that impaired processing was genetically complemented, suggesting that the processing defect is a recessive trait. Three distinct processing phenotypes were observed …

A Biosystematic Study Of Three Plant Pathogenic Fungi, Richard James Langham

Masters Theses

Isolates of Chalara australis, Eucalypt Ceratocystis, and Ceratocystis virescens were grown on Potato Dextrose Agar, Malt Extract Agar, and Czapek Agar and examined as part of a biosystematic study on these fungi. Basic micromorphological and macromorphological data on the cultures were recorded and the taxa were found to be morphologically similar. Tissue isolates and single spore isolates were crossed within these taxa and an interspecific cross performed. Chalara australis was found to be either asexual or have only one mating type present in the cultures studied. Eucalypt Ceratocystis was found to have a mating system characteristic of bipolar …

Relative Sensitivities Of Diatoms To Selected Heavy Metals, Jennifer E. Carlson

Masters Theses

Baseline data, including diatom community structure, metal concentrations of water, sediments and periphyton of the Embarras River were determined previously (Vaultonburg, 1991). Data on diatom community structure in conjunction with efforts to identify relative sensitivity of diatoms may be useful in establishing an effective method for biomonitoring metal pollution in the Embarras River and other aquatic systems. The main objectives of this project were: i) develop a bioassay method; ii) evaluate relative toxicities of several metals towards diatoms; and, iii) evaluate relative sensitivities of diatoms toward dissolved metals.

Unialgal cultures of Cyclotella meneghiniana, Navicula vaucheriae, and Nitzschia palea were isolated …

Frequency Of Mug Negative Escherichia Coli In Kentucky Groundwater Samples, Mark S. Coyne, J. C. Shuler

Plant and Soil Sciences Faculty Publications

MUG negative Escherichia coli are a small fraction (2.5%) of the total E. coli in Kentucky groundwater samples. It is unlikely that they alone will cause a significant potential to underestimate fecal contamination using MUG as the primary criterion for that assessment. An unresolved question is how effectively MUG-based, defined-substrate tests address false negative water samples containing MUG positive E. coli.

Vh And D Gene Usage In B-Lineage Cells From Young Rabbits, Martha L. Friedman

Master's Theses

No abstract provided.

Development And Application Of A Thermistor Current Meter, Carl M. Way, Albert J. Burky, Christine Miller-Way

Biology Faculty Publications

This report provides details for the construction of a hot-bead thermistor current meter which is capable of measuring water velocities on a millimeter spatial scale and for the construction of a compact and accurate calibration system. Hot-bead thermistor current meters can be built with response times of 200 ms capable of measuring velocities between 0.1 and 80 cm s-1. The construction of a sturdy probe for application in lotic systems such as high gradient Hawaiian streams was achieved by the use of heavy-duty acrylic tubing, small stainless steel gas-chromatography tubing, and flexible Tygon spaghetti tubing. An acrylic handle anchors the …

Identification, Enrichment, And Cytokine Production Of Murine Bone Marrow Stromal Cells, Phillip E. Funk

Dissertations

No abstract provided.

Nonhomologous Rna Recombination In Tombusviruses: Generation And Evolution Of Defective Interfering Rnas By Stepwise Deletions, K. Andrew White, Thomas Jack Morris

Nebraska Center for Virology: Faculty Publications

We used a protoplast system to study the mechanisms involved in the generation and evolution of defective interfering (DI) RNAs of tomato bushy stunt tombusvirus (TBSV). Synthetic transcripts corresponding to different naturally occurring TBSV DI RNAs, or to various artificially constructed TBSV defective RNAs, were analyzed. The relative levels of competitiveness of different DI RNAs were determined by coinoculating their corresponding transcripts into protoplasts along with helper genomic RNA transcripts and monitoring the level of DI RNA accumulation. Further studies were performed to assess the contribution of naked DI RNA stability and DI RNA encapsidation efficiency to the observed levels …