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Articles 31 - 48 of 48
Full-Text Articles in Microbiology
Antibacterial Activities Of The Extracts Of Allium Sativum (Garlic) And Allium Cepa (Onion) Against Selected Pathogenic Bacteria, Sherifat O. Enejiyon, Al-Hassan A. Abdulrahman, Abdulameen S. Adedeji, Ramatu Abdulsalam, Mary U. Oyedum
Antibacterial Activities Of The Extracts Of Allium Sativum (Garlic) And Allium Cepa (Onion) Against Selected Pathogenic Bacteria, Sherifat O. Enejiyon, Al-Hassan A. Abdulrahman, Abdulameen S. Adedeji, Ramatu Abdulsalam, Mary U. Oyedum
Tanzania Journal of Science
Although many antibiotics have been developed against bacterial diseases, the problems of antibiotic resistance and toxicity have made the continuous search for newer drugs an existential necessity. This study determined the phytochemical components and antibacterial activities of Allium sativum and Allium cepa against Salmonella typhi, Staphylococcus aureus, Streptococcus pneumoniae, Klebsiella pneumoniae and Escherichia coli. The phytochemical analysis was carried out using standard methods. The antibacterial activities of the aqueous, ethanol and acetone extracts of both plants at different concentrations were determined against all the test organisms using agar well diffusion method. All the extracts were found to contain important phytochemicals …
Dengue Vector Distribution And Their Infection Status In Selected Regions In Tanzania, Anitha Philbert, Amina Msonga
Dengue Vector Distribution And Their Infection Status In Selected Regions In Tanzania, Anitha Philbert, Amina Msonga
Tanzania Journal of Science
Dengue fever is an important arboviral infection globally. Tanzania has experienced multiple dengue outbreaks since 2010 leading to mortalities and public distress. Dengue is caused by viruses which are transmitted to humans by vector mosquitoes. Nevertheless, the abundance, distribution and extent of viral infections of these vectors are poorly understood. The aim of this study was to characterize dengue vectors and determine their virus infection rates. Adult mosquitoes were collected from selected regions in Tanzania including Dar es Salaam, Tanga, Coast, and Arusha regions using CO2 baited mosquito magnet traps between December 2018 to February 2019, additional samples were collected …
Assessment Of Biochemical Changes During Fermentation Process For Production Of Traditional Fermented Cassava Meal “Mchuchume”, Severino Alphonce, Lilian Kaale
Assessment Of Biochemical Changes During Fermentation Process For Production Of Traditional Fermented Cassava Meal “Mchuchume”, Severino Alphonce, Lilian Kaale
Tanzania Journal of Science
“Mchuchume” is a ready to eat fermented cassava food whose production is accomplished by a consortium of microorganisms, some of which may be probiotic in nature and others pathogens to humans and of no use in the fermenting system. This work made characterization of biochemical changes evolved at different times in local (open) and biotechnological (closed) fermentation methods. The study also assessed organoleptic properties of final products. Acidification was observed in the retting water as pH ranged from 6.32 to 4.25 and titratable acidity from 0.02% to 0.32%. A decrease in total reducing sugars (6.59 to 4.03 mg/g) in fermenting …
Current Epidemiological Assessment Of Plasmodium Falciparum And Helminth Co-Infections In Children After A Decade Of Implementation Of Control Programs In Morogoro Region, Tanzania, Christopher Kisiringyo, Winifrida Kidima
Current Epidemiological Assessment Of Plasmodium Falciparum And Helminth Co-Infections In Children After A Decade Of Implementation Of Control Programs In Morogoro Region, Tanzania, Christopher Kisiringyo, Winifrida Kidima
Tanzania Journal of Science
A school based cross-sectional study was conducted from July to November 2018 to assess the burden of asymptomatic Plasmodium falciparum, Schistosoma and soil transmitted helminth (STH) infections in Mvomero. A total of 374 children (age range = 5–16 years, mean age = 11.3 years) were recruited from five primary schools. Prevalence of asymptomatic P. falciparum infections were 29.9%, S. haematobium 49.7%, hookworm 20.3%, Ascaris lumbricoides 12.6%, Taenia saginata 0.5% and S. mansoni 0.3%. Malaria parasite density increased with increasing children age (r = 0.99). Only 6.5% (12/186) of S. haematobium infected children were presented with heavy infections, whereas all STH-positive …
Geochemical Characteristics Of Soil In Relation To Occurrence Of Entomopathogenic Nematodes (Epns) In Osogbo, Osun State, Nigeria, Mohammed Rufai, Abidat Shittu, Monsuru Adeleke
Geochemical Characteristics Of Soil In Relation To Occurrence Of Entomopathogenic Nematodes (Epns) In Osogbo, Osun State, Nigeria, Mohammed Rufai, Abidat Shittu, Monsuru Adeleke
Tanzania Journal of Science
The occurrence and abundance of entomopathogenic nematodes (EPNs) vary across locations, and this has been attributed to many factors including soil physico-chemical properties. This study examined the influence of selected soil chemicals on the occurrence of EPNs under different cultivated lands in Osun state, Southwestern Nigeria. EPNs were isolated from 23.33% of the soil samples in selected sites using decantation and centrifugation method and nematodes observed under standard microscopy were identified based on morphological analysis of infective juveniles (IJs). This study has revealed the presence of nematodes of the families Steinernema and Heterorhabditis in the sampled soils. Soil chemical properties, …
Epidemiological Significance Of Parasitic Infections In Olive Baboons (Papio Anubis) At Gombe National Park, Tanzania, Jared S. Bakuza
Epidemiological Significance Of Parasitic Infections In Olive Baboons (Papio Anubis) At Gombe National Park, Tanzania, Jared S. Bakuza
Tanzania Journal of Science
Several parasites of significant health importance have been observed in baboons at Gombe National Park in western Tanzania. However, little is known on impacts these infections have on baboon health. Baboon stools were examined for parasites using formol-ethyl technique and their health and troop membership associated with parasitic infections. Observed nematode parasites were; Streptopharagus sp., Physaloptera sp., unidentified nematodes, hookworms and Trichuris sp. Trematode parasites included Schistosoma mansoni and unidentified trematode, with the latter’s intensity being highest in DB troop baboons. Parasite egg counts (epg) for nematodes ranged from 9 to 12, and that of trematodes from 4 to 40 …
An Investigation Of The Potentials Of 2-[(2-Hydroxyphenyl) Methylidene] Hydrazine-1-Carbothioamide And Its Mn(Ii) And Zn(Ii) Complexes As Antimicrobial Agents, Umar Sani, Musbahu Yahaya, Dailami S. Adam
An Investigation Of The Potentials Of 2-[(2-Hydroxyphenyl) Methylidene] Hydrazine-1-Carbothioamide And Its Mn(Ii) And Zn(Ii) Complexes As Antimicrobial Agents, Umar Sani, Musbahu Yahaya, Dailami S. Adam
Tanzania Journal of Science
Antibiotic resistance as a result of misuse, overuse or abuse of drugs is an issue of serious concern world over. The quest for more active and robust antimicrobial agents remains a scientific challenge and requires multimillion-dollar investment in the area of drug design and development. Here in, we report the synthesis and characterisation of a tridentate thiosemicarbazone (2-[(2-hydroxyphenyl)methylidene]hydrazine-1-carbothioamide) (HL) from salicylaldehyde and thiosemicarbazide. Its complexes with Mn(II) and Zn(II) were prepared following similar technique and characterised by melting point, FT-IR, UV –visible spectrophotometry, and elemental analysis. The Mn(II) complex showed paramagnetism with a magnetic moment value of 5.80 BM while …
In Vivo Antimycobacterial Studies Of Toussaintine A-Chitosan Nanocomposites, Edward Rwegasila, Joan J.E. Munissi, Egid B. Mubofu, Stephen S. Nyandoro, Paul Erasto
In Vivo Antimycobacterial Studies Of Toussaintine A-Chitosan Nanocomposites, Edward Rwegasila, Joan J.E. Munissi, Egid B. Mubofu, Stephen S. Nyandoro, Paul Erasto
Tanzania Journal of Science
Chitosan (CS, molecular weight (MW) 20.2 kDA, stability of 210 °C and degree of deacetylation (DD) 73.31%) was obtained by deacetylation of chitin extracted from shrimp (Litopenaeus vannamei) shell wastes. The encapsulation of the studied bioactive natural product, toussaintine A (TA) isolated from the leaves of Toussaintia orientalis, on a chitosan-tripolyphosphate (CS/TPP) nanoformulation was attained through ionotropic gelation. Characterization of pure CS, CS/TPP and TA-CS/TPP nanocomposites was carried out by FTIR and SEM. The encapsulation efficiency and loading capacity of the TA were 69.33 and 0.46%, respectively. The in vitro release kinetics established an initial release of 27% of TA …
In Vivo Antimycobacterial Studies Of Toussaintine A-Chitosan Nanocomposites, Edward Rwegasila, Joan J.E. Munissi, Egid B. Mubofu, Stephen S. Nyandoro, Paul Erasto
In Vivo Antimycobacterial Studies Of Toussaintine A-Chitosan Nanocomposites, Edward Rwegasila, Joan J.E. Munissi, Egid B. Mubofu, Stephen S. Nyandoro, Paul Erasto
Tanzania Journal of Science
Chitosan (CS, molecular weight (MW) 20.2 kDA, stability of 210 °C and degree of deacetylation (DD) 73.31%) was obtained by deacetylation of chitin extracted from shrimp (Litopenaeus vannamei) shell wastes. The encapsulation of the studied bioactive natural product, toussaintine A (TA) isolated from the leaves of Toussaintia orientalis, on a chitosan-tripolyphosphate (CS/TPP) nanoformulation was attained through ionotropic gelation. Characterization of pure CS, CS/TPP and TA-CS/TPP nanocomposites was carried out by FTIR and SEM. The encapsulation efficiency and loading capacity of the TA were 69.33 and 0.46%, respectively. The in vitro release kinetics established an initial release of 27% of TA …
Occurrence Of Pathogenic Vibrio Cholerae Serogroups O1 And O139 In Some Estuaries Of Tanzania, David Temba, Lucy Namkinga, Sabrina Moyo, Thomas J. Lyimo, Charles V. Lugomela
Occurrence Of Pathogenic Vibrio Cholerae Serogroups O1 And O139 In Some Estuaries Of Tanzania, David Temba, Lucy Namkinga, Sabrina Moyo, Thomas J. Lyimo, Charles V. Lugomela
Tanzania Journal of Science
In this study, the occurrence of culturable Vibrio cholerae serogroup O1 and O139 in three selected Tanzanian Estuaries, Mzinga, Pangani and Ruvu were explored. A total of 480 water and plankton samples were collected along salinity gradients (fresh, brackish and marine waters). Samples were enriched in Alkaline Peptone Water (APW) and subsequently cultured on Thiosulfate Citrate Bile-Salt Sucrose (TCBS) agar. Resulting isolates were serially identified by oxidase test, API-20E test and monoclonal antibodies for V. cholerae serogroup O1 and O139. A total of 670 presumptive V. cholerae isolates were obtained from 416 samples (86.7%), where 137 (20.4%) were oxidase test …
Cytotoxicity, Antimicrobial And Antioxidant Activities Of Boletus Bicolor, A Basidiomycetes Mushroom Indigenous To Tanzania, Donatha Tibuhwa
Cytotoxicity, Antimicrobial And Antioxidant Activities Of Boletus Bicolor, A Basidiomycetes Mushroom Indigenous To Tanzania, Donatha Tibuhwa
Tanzania Journal of Science
Boletus bicolor commonly known as coloured bolete is a basidiomyctes mushroom species widely used as a delicacy and its extract has been used traditionally to treat several human ailments in Tanzania. This study evaluates its extracts in order to provide a scientific foundation on the possible application in folk medicine system based on the biochemical evaluation. Standard microbes Bacillus subtilis and Candida albicans were used to test, the antifungal and antibacterial activity. Cytotoxicity test was also done on the extracts using brine shrimp test. Besides, the extracts were also used to investigate for antioxidant activity using DPPH (1, 1-diphenyl-2-picrylhydrazyl radical). …
Ligninolytic Enzymes Of The Fungus Isolated From Soil Contaminated With Cow Dung, Rose J. Masalu
Ligninolytic Enzymes Of The Fungus Isolated From Soil Contaminated With Cow Dung, Rose J. Masalu
Tanzania Journal of Science
Lignin is the complex polymer and very few microorganisms are able to degrade it. The study aimed at isolating lignin degrading fungi from soil contaminated with cow dung using ligninolytic screening media. The study was able to isolate one ligninolytic fungus by using lignin media. The strain was screened for production of ligninolytic enzymes using Rhemazol Brilliant blue R (RBBR) dye, 2,2-azino-bis (3- ethylbenzthiazoline)-6-sulfonate (ABTS) and guaiacol in a semi-solid medium. The isolate decolourized RBBR dye, ABTS and guaiacol in a semi solid media. The strain was further tested for lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase (Lac) enzymatic …
Antimycobacterial And Cytotoxicity Evaluation Of The Constituents Of Monodora Carolinae, Nyangi Magori, Stephen S Nyandoro, Joan Je Munissi, Matthias Heydenreich
Antimycobacterial And Cytotoxicity Evaluation Of The Constituents Of Monodora Carolinae, Nyangi Magori, Stephen S Nyandoro, Joan Je Munissi, Matthias Heydenreich
Tanzania Journal of Science
Phytochemical investigation of the stem bark of the recently described species Monodora carolinae (Annonaceae) afforded prenylindole derivatives (E)-4-(1H-indol-6-yl)-but-3-en-2-one (1), 5-formylindole (2), fatty acid (Z)-hexade-7-enoic acid (3) and the lignanamide cannabisin B (4). The structures of the isolated compounds were established using NMR and MS analyses. The antimycobacterial activities of the extracts and isolated compounds were evaluated against Mycobacterium madagascariense (MM) and M. indicus pranii (MIP) using the two-fold microtitre dilution method. While the extracts exhibited minimum inhibitory concentration (MIC) ranging from 500 – 1000 µg/mL, the isolated compounds were 125 – 250 µg/mL, indicating very low activity. Cytotoxic effects were …
Composition Of Non Volatile Oils And Antimicrobial Activities Of Extracts From Monanthotaxis Discolor, And An Undescribed Uvariondedron Species, Ds Parmena, Ca Mgina, Cc Joseph
Composition Of Non Volatile Oils And Antimicrobial Activities Of Extracts From Monanthotaxis Discolor, And An Undescribed Uvariondedron Species, Ds Parmena, Ca Mgina, Cc Joseph
Tanzania Journal of Science
The chemical compositions of non volatile oil extracts from two Annoneceous plant species Monanthotaxis discolor and an undescribed Uvariodendron species which are endemic to Eastern Arc Mountains, Tanzania were determined by GC-MS. The biological activities of extracts and fractionated samples were also determined. Both methanol and dichloromethane extracts of the root bark of M. discolor showed mild antimicrobial activity and had positive brine shrimp test (BST). The BST test for dichloromethane extract of M. discolor root bark were LC50 41.794 μg/ml and that of methanol extract showed LC50 13.560 μg/ml. The petroleum ether and dichloromethane extracts of root bark of …
Characteristics And Dyes Biodegradation Potential Of Crude Lignolytic Enzymes From White-Rot Fungus Crepidotus Variabilis Isolated In Coastal Tanzania, G. Y. S. Gys Mtui
Characteristics And Dyes Biodegradation Potential Of Crude Lignolytic Enzymes From White-Rot Fungus Crepidotus Variabilis Isolated In Coastal Tanzania, G. Y. S. Gys Mtui
Tanzania Journal of Science
Lignocellulosic enzymes from Crepidotus variabilis collected from mangrove forests of coastal Tanzania were investigated by using standard methods, and their ability to degrade aromatic compounds were elucidated. The fungal crude enzyme filtrates had maximum laccase (Lac), lignin peroxidase (LiP) and manganese peroxidase (MnP) activities of 70 U/mL, 16 U/mL and 8, U/mL respectively. The crude enzyme extracts were able to oxidize rhemazol brilliant blue-R (RBB-R) dye, phenol, "-naphthol and pyrogallol. Also, they could remove up to 58% and 92% color from raw textile effluent and aromatic dyes, respectively, after 14 days of incubation at 30ºC and pH 4.5. Desalted and …
Antibacterial Activities Of Constituents From Isolona Cauliflora And Cleistochlamys Kirkii, Ac Odebode, Sjm Mdachi, Cc Joseph, Bn Irungu
Antibacterial Activities Of Constituents From Isolona Cauliflora And Cleistochlamys Kirkii, Ac Odebode, Sjm Mdachi, Cc Joseph, Bn Irungu
Tanzania Journal of Science
Antibacterial activities of caulindole D, a mixture of caulindole E and F, pinocembrin and an oxyheptanoid (cleistenolide), obtained from stem barks of Isolona cauliflora and Cleistochlamys kirkii, on Staphylococcus aureus and Pseudomonas phaseolicola were determined. An in vitro assay test by paper disc method showed that the caulindole D, E and F from Isolona cauliflora produced no inhibition zone on both bacteria pathogens at 100-200 ppm while cleistochlamic acid from C. kirkii showed weak to moderate inhibition zone at 100-200 ppm. At concentration above 200 ppm, the bacteria pathogens were inhibited by all the compounds isolated from the plant extracts. …
Cultivating The Edible Oyster Mushroom Pleurotus On Sawdust, Fss Magingo
Cultivating The Edible Oyster Mushroom Pleurotus On Sawdust, Fss Magingo
Tanzania Journal of Science
The edible mushroom Pleurotus pulmonarius was grown on pasteurized saw dust supplemented with either Crotalaria ochroleuca (N2- fixing legume) or with chicken manure. Inoculated, unsupplemented sawdust acted as control. Sawdust supplemented with C. ochroleuca biomass yielded the highest fresh weight of the mushroom with a biological efficiency (B.E) of 83%. Sawdust supplemented with chicken manure had a 67% B.E., while control gave the least harvest (B.E. = 32%). Reasons for the differences are discussed.
Effect Of Filter Pore Size For Liquid Effluent Removal On The Performance Of An Artificial Rumen Reactor, Amelia Kajumulo Kivaisi, Martin Barugahare
Effect Of Filter Pore Size For Liquid Effluent Removal On The Performance Of An Artificial Rumen Reactor, Amelia Kajumulo Kivaisi, Martin Barugahare
Tanzania Journal of Science
The effect of filter pore size on performance of an artificial rumen reactor was studied. Filter units of pore sizes in the range of 10-300 mm were used in the reactor to separate the solids from the fermentation liquid at solid and liquid retention times of 72 and 12 h, respectively. The overall performance of the reactor decrease markedly with increasing filter pore size. Total cellulose activity and substrate degradation decreased by 70 and 30% respectively, when the pre sizes changed from 10 to 300 mm. ciliate protozoa decreased in number with increasing filter pore size and were almost completely …