Microbiology Commons^™

Molecular Analysis Reveals Unique Microbiome In Ileal Pouch During Pouchitis Compared To Healthy Pouches In Ulcerative Colitis And Familial Adenomatous Polyposis, Tiffany Wallingford Glavan

Master's Theses

In severe cases of ulcerative colitis (UC) unresponsive to current treatment options, patients require a complete proctocolectomy, or surgical removal of the colon. Ileal pouch anal anastomosis (IPAA) has become the preferred surgical technique for patients who require surgery, as this method restores rectal function. This procedure is also used to treat colorectal cancers such as adenocarcinoma and familial adenomatous polyposis (FAP). The surgery involves an abdominal colectomy with the construction of an ileal pouch created from folded tissue recovered from the ileal portion of the small intestine. Up to 50% of patients who require IPAA surgery experience an episode …

Pcr Primers For The Detection Of Propane And Butane-Oxidizing Microorganisms, Brian Jeremy Chan

Master's Theses

In an increasingly energy-hungry world, our capacity to meet the heightened energy demands of the future has become a pressing matter. The most urgent of these concerns are tied to the accessibility of petroleum. Various experts have proselytized both the imminent arrival of peak oil production rates and the ensuing decline of those rates thereafter. And to that end, the development of novel and advanced oil exploration methodologies has become almost as important as finding the sources of oil themselves.

The soils above petroleum reservoirs play host to various communities of alkane- oxidizing bacteria that can utilize the natural gas …

Detection Of The Sire1 Env-Like Protein, A Potential Retroviral Protein In Soybean, Garen Gaston

Master's Theses

Long terminal repeat (LTR) retroelements are mobile DNAs that are ubiquitous in eukaryotic genomes and include retroviruses, endogenous retroviruses and retrotransposons. Though retrotransposons, which are noninfectious, can make up as much as 80% of plant genomes, no traces of retroviral elements have been confirmed in plants. However, the 2000-copy soybean retrotransposon SIRE1 has an extra open reading frame, that has been described as env-like, because its conceptual translation product is predicted to have features of retroviral envelope proteins, which sponsor infectivity. Demonstrating that this region encodes an envelope protein would extend the range of endogenous retroviruses to plants. Demonstrating also …

Role Of Type Ii Transmembrane Serine Proteases In Coronavirus Production, Gitanjali Cuckemane Subramanya

Master's Theses

Proteolytic cleavage of Coronavirus spike proteins at the appropriate time and location results in efficient virus entry activation. Type II transmembrane serine proteases (TTSPs), specifically, transmembrane protease serine 2 (TMPRSS2) when expressed on target cells, enhance SARS coronavirus entry by activating spike cleavage. This study investigated the effect of TTSPs expressed in Coronavirus producer cells. Murine Hepatitis Virus strain A59 (MHV A59) viruses produced in the absence of TMPRSS2 required target cell protease activity - either cell surface serine proteases or endosomal acidophilic proteases - for entry. MHV A59 viruses produced in the presence of TMPRSS2 were less infectious overall, …

Designing A Microbial Prolyl Peptidase Delivery System For The Treatment Of Celiac Disease, Matthew J. Shurtleff

Master's Theses

Celiac disease is an autoimmune enteropathy resulting from the ingestion of gluten and gluten-like proteins from wheat, barley and rye in afflicted individuals. Indigestible gluten-derived peptides rich in proline residues are known to be responsible for eliciting the inappropriate immune response characteristic of the disease. In this investigation, surface level expression of prolyl peptidase activity by genetically engineered probiotic lactobacilli was postulated to be a possible treatment for this disease. Plasmid-based reporter vectors were constructed utilizing a novel, homology-based cloning technique to assess the expression and localization signals from the S-layer protein gene of Lactobacillus acidophilus. These plasmids were mutated …

Temperature Influence And Heat Management Requirements Of Microalgae Cultivation In Photobioreactors, Thomas Hagen Mehlitz

Master's Theses

Microalgae are considered one of the most promising feedstocks for biofuel production for the future. The most efficient way to produce vast amounts of algal biomass is the use of closed tubular photobioreactors (PBR). The heat requirement for a given system is a major concern since the best algae growth rates are obtained between 25-30 °C, depending on the specific strain. A procedure to determine temperature influence on algal growth rates was developed for a lab-scale PBR system using the species Chlorella. A maximum growth rate of 1.44 doublings per day at 29 °C (optimal temperature) was determined. In addition, …

Effects Of Rearing Environment On The Somatic Diversification Of Rabbit Immunoglobulin Vdj Genes, David P. Brashinger

Master's Theses

No abstract provided.

Vh And D Gene Usage In B-Lineage Cells From Young Rabbits, Martha L. Friedman

Master's Theses

No abstract provided.

The Inhibitory Effects Of The Extracts Of Zingiber Plants On The Adsorption, Growth, And Replication Of Phage Lpp-1 In Cyanobacterium, Ebby Paul Jido

Master's Theses

No abstract provided.

Quantitation Of Type I Collagen Mrna In Intact Human Fibroblasts, Edward Touma

Master's Theses

No abstract provided.

Comparative Extracellular Killing Of Candida And Tumor Cells By Macrophages At Varying Stages Of Activation, David W. A. Beno

Master's Theses

No abstract provided.

Factors Affecting Germ Tube Formation In Candida Albicans, Walter A. Tatarowicz

Master's Theses

No abstract provided.

Observations On The Adherence Of Bacteroides Bivius To Female Genital Tract And Oral Epithelial Cells, Susan V. Meade

Master's Theses

No abstract provided.

Development Of A Dna Probe For The Rapid Detection Of Cytomegalovirus, Nell Lurain

Master's Theses

No abstract provided.

Chemical And Ultrastructural Characterization Of Trichophyton Mentagrophytes Arthrospore Walls And Septa, Jordan H. Pollack

Master's Theses

No abstract provided.

The In Vitro And In Vivo Effects Of Sm-1213 Drug Therapy On Pathogenesis And Host Defense In Experimentally Induced Candidiasis, Christine Joy Morrison

Master's Theses

No abstract provided.

Characterization Of A Multiple Antibiotic Resistance Plasmid From Proteus Mirabilis, Camille Talanda-Fath

Master's Theses

No abstract provided.

Effects Of Sodium Deoxycholate On Selected Gram-Negative Bacteria, Anthony Paul D'Mello

Master's Theses

No abstract provided.

Reactivity Of Antibodies Versus Streptococcal Carbohydrate And Protein Antigens And Human Glomerular Basement Membrane Carbohydrate And Protein Antigens In Mammalian Tissues, Kathleen A. Chase

Master's Theses

No abstract provided.

Characterization Of Pseudomonas Cepacia Using Biochemical, Antibiotic Susceptibility And Serological Tests, Beatrice Grazina Miceika

Master's Theses

No abstract provided.

Characterization Of The Nonfermentative Gram Negative Rods Of Group Iik Utilizing Biochemical, Serological, And Antibiotic Susceptibility Tests, Donna S. Granstrom

Master's Theses

No abstract provided.

The Biosynthesis Of Mannitol And Mannitol 1-Phosphate In Staphylococci, Kenneth Edwards

Master's Theses

No abstract provided.

Some Environmental Factors Affecting The Morphology Of Streptococcus Mutans 10449, Ruth Gail Emyanitoff

Master's Theses

No abstract provided.

Interspecific Homology Between Ribosomal Proteins: The Characterization Of A 30 S Ribosomal Protein From Bacillus Stearothermophilus Which Functionally Replaces Escherichia Coli S 19, Artemios W. Vassos

Master's Theses

No abstract provided.

The Immunological Response Of A Freshwater Fish To Bacteria Of Marine Origin, Robert S. Pekarek

Master's Theses

No abstract provided.

The Effects Of Herbicides, Fungicides, And Insecticides On Bacteria Of Marine Origin, Kenneth William Pierini

Master's Theses

No abstract provided.

The Influence Of Chemicals On The Growth Of Bacteria, Alice J. Burke

Master's Theses

No abstract provided.