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University of Arkansas, Fayetteville

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Articles 181 - 198 of 198

Full-Text Articles in Microbiology

Efficient Separation And Sensitive Detection Of Listeria Monocytogenes Using Magnetic Nanoparticles, Microfluidics And Interdigitated Microelectrode Based Impedance Immunosensor, Damira Ashimkhanovna Kanayeva Dec 2010

Efficient Separation And Sensitive Detection Of Listeria Monocytogenes Using Magnetic Nanoparticles, Microfluidics And Interdigitated Microelectrode Based Impedance Immunosensor, Damira Ashimkhanovna Kanayeva

Graduate Theses and Dissertations

Listeria monocytogenes continues to be a major foodborne pathogen that causes food poisoning and sometimes death in immunosuppressed people and abortion in pregnant women. Nanoparticles have recently drawn attentions for use in immunomagnetic separation techniques due to their greater surface area/volume ratio and better stability against sedimentation in the absence of a magnetic field. Interdigitated microelectrodes and microfluidics make material transfer more efficient and biological/chemical interaction between the surface and solution phase much quicker. Magnetic nanoparticles (Fe3O4) with a 30 nm diameter were functionalized with rabbit anti-L. monocytogenes antibodies via biotin-streptavidin bonds and then amalgamated with target bacterial cells …


Biogeography And Microhabitat Distribution Of Myxomycetes In High-Elevation Areas Of The Neotropics, Carlos Alonso Rojas-Alvarado May 2010

Biogeography And Microhabitat Distribution Of Myxomycetes In High-Elevation Areas Of The Neotropics, Carlos Alonso Rojas-Alvarado

Graduate Theses and Dissertations

Myxomycetes are a group of amoeboid organisms with the capacity of forming fruiting bodies that resemble some macrofungi. The ecology and global distribution of species within the group have been studied only during the last half century. For this reason, a number of questions regarding the nature of the interactions that exist between myxomycetes and their environment still lack the empirical evidence required to obtain complete answers. In the Neotropical region, species assemblages have been moderately well studied, but their biogeography and macroecology have received little attention. In high-elevation areas of this region, the situation is especially precarious, due the …


Letter From The Dean, Lalit Verma Jan 2009

Letter From The Dean, Lalit Verma

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.


Design Of A Bioreactor To Study The Role Of Red Blood Cells In The Transport Of Nitric Oxide In The Microcirculation, Nupura Bhise, Mahendra Kavdia Jan 2007

Design Of A Bioreactor To Study The Role Of Red Blood Cells In The Transport Of Nitric Oxide In The Microcirculation, Nupura Bhise, Mahendra Kavdia

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Nitric oxide (NO) plays an important role in physiological functions like vasodilation, neurotransmission, and inhibition of platelet aggregation. The endothelium-derived NO diffuses into the vascular lumen where it interacts with flowing blood as well as the smooth muscles where it modulates vascular tone. However, uncertainty exists on how NO escapes the rapid scavenging by hemoglobin (Hb) and reaches smooth muscles. Several proposed hypotheses include 1) a reduced reaction rate of NO with Hb contained inside red blood cells (RBCs) and 2) NO preservation in the bound form of s-nitrosohemoglobin or nitrite. The mechanism and magnitude of reduction of NO reaction …


Transcriptional Control Of The Opp Operon In Clostridium Difficile, S. Lindsey Davis Jan 2003

Transcriptional Control Of The Opp Operon In Clostridium Difficile, S. Lindsey Davis

Inquiry: The University of Arkansas Undergraduate Research Journal

As a serious expense for both the hospital and the patient, nosocomial infections create a burden on the health care industry that is not easily overcome. Among the infections commonly contracted in the hospital environment, those associated with the Clostridium difficile bacterium account for millions of cases each year. Largely due to the nature of C. difficile infection as a response to the disruption of the normal flora of the colon caused by antibiotic activity, no completely effective treatment for this condition has been identified. It is this problem that forms the foundation for research devoted to the development of …


Use Of Irradiated And Formalin-Fixed Trichomonas Vaginalis To Examine Protective Immune Responses In The Mouse Intraperitoneal Model, Terryl L. Hostetler, James J. Daly, Timothy J. O'Brien, Max L. Baker Jan 2003

Use Of Irradiated And Formalin-Fixed Trichomonas Vaginalis To Examine Protective Immune Responses In The Mouse Intraperitoneal Model, Terryl L. Hostetler, James J. Daly, Timothy J. O'Brien, Max L. Baker

Journal of the Arkansas Academy of Science

Gamma irradiated Trichomonas vaginalis was used to initiate a protective immune response in mice by intraperitoneal inoculation using host mortality as the measure of virulence. Irradiated trichomonads of a virulent strain gave some immune protection but no more so than the use of formalin-fixed, virulent trichomonads. A formalin-fixed virulent strain combined with complete Freund's adjuvant (CFA) gave complete protection. Metabolically produced antigens do not appear to be important in conferring protective immunity in these experiments. A common laboratory strain of T. vaginalis (ATCC 30001) was used as an avirulent control. It gave no protection against a virulent strain. Combining CFA …


In Vitro Growth Characteristics Of Two Cryptococcus Neoformans Isolates, Kristy L. Jones, Juneann W. Murphy Jan 1998

In Vitro Growth Characteristics Of Two Cryptococcus Neoformans Isolates, Kristy L. Jones, Juneann W. Murphy

Journal of the Arkansas Academy of Science

Cryptococcus neoformans is an opportunistic pathogen which attacks individuals with weakened immune systems. Two C. neoformans isolates, 184A and NU-2, were examined to determine characteristics that contribute to their difference in virulence. Both isolates were grown under tissue culture conditions, and different characteristics were tested at timed intervals. Isolate 184A was found to grow at a faster rate than isolate NU-2, with doubling times of 4 and 12 hours respectively. The polysaccharide capsule of isolate NU-2 doubled in size in four hours, while that of isolate 184A stayed approximately the same. The NU-2 yeast cell diameter without the polysaccharide capsule …


Non-Measles Hemadsorption In A Cell Line Persistently Infected With Measles Virus (Bgm/Mv), Jay H. Menna, John D. May Jan 1981

Non-Measles Hemadsorption In A Cell Line Persistently Infected With Measles Virus (Bgm/Mv), Jay H. Menna, John D. May

Journal of the Arkansas Academy of Science

Adsorption of Rhesus monkey erythrocytes to the plasma membranes of measles virus infected cells is frequently carried out to detect the presence of plasma membrane-associated measles virus hemagglutinin. The hemagglutinin is a viral genome-coded structural glycoprotein of the measles virion that is associated with the plasma membrane of the host cell during measles virus replication. BGM/MV, anon-virogenic line of African green monkey kidney cells persistently-infected with measles virus, adsorbed Rhesus monkey erythrocytes in an inverse fashion relative to the number of cells present in the culture and the time post-seeding. Serological studies employing the hemadsorption-inhibition and membrane immunofluorescence assay procedures, …


Distribution Of Naegleria Fowleri In Selected Northeast Arkansas Lakes, Robert Dean Evans, Lawrence W. Hinck Jan 1980

Distribution Of Naegleria Fowleri In Selected Northeast Arkansas Lakes, Robert Dean Evans, Lawrence W. Hinck

Journal of the Arkansas Academy of Science

Seven northeast Arkansas recreational lakes were examined for the presence of pathogenic and nonpathogenic Naegleria fowleri. Cultural differentiation and microscopic morphology were used as species determining tests, while mouse pathogenicity tests were conducted to determine virulence. Only one isolate met all criteria utilized for definite identification of Naegleria fowleri, although Naegleria type organisms were found in all of the lakes. None of the isolates were pathogenic in mice.


Effect Of Cold Shock On The Metabolism Of Trichomonas Gallinae, James J. Daly Jan 1980

Effect Of Cold Shock On The Metabolism Of Trichomonas Gallinae, James J. Daly

Journal of the Arkansas Academy of Science

The influence of cold (4°C) used to prepare cells for metabolic investigations was examined with Trichomonas gallinae in this study. Cells washed with cold diluent in a refrigerated centrifuge were found to be less stimulated in their gas production at 37°C when exposed to glucose or maltose than cells washed at room temperature conditions. Such cold-prepared cells had higher initial glycogen contents after washing, faster endogenous glycogen degradation rates when incubated at 37 °C, but lower glycogen synthesis in the presence of glucose or maltose when compared to cells not prepared in the cold. However, uptake of glucose and maltose …


Presence Of An Unusual Eosinophilic Staining Substance In The Blood Of Snakes, James J. Daly Sr., Charles H. Calhoun Jan 1978

Presence Of An Unusual Eosinophilic Staining Substance In The Blood Of Snakes, James J. Daly Sr., Charles H. Calhoun

Journal of the Arkansas Academy of Science

No abstract provided.


Nonpathogenic Free-Living Amoebae In Arkansas Recreational Waters, Leon W. Bone, David A. Becker Jan 1975

Nonpathogenic Free-Living Amoebae In Arkansas Recreational Waters, Leon W. Bone, David A. Becker

Journal of the Arkansas Academy of Science

Selected recreational waters of Arkansas were sampled for pathogenic free-living limax amoebae. Water quality parameters were determined for correlation with amoebic population densities and species diversity. Cultural criteria and animal inoculation revealed no pathogenic strains. The possibility of introduction and/or induction of pathogenic amoebic strains by environmental factors requires further ecological investigations.


Facility For The Biological Treatment Of A Complex Chlorophenolic Waste - A Preliminary Report, William F. Evans Jan 1971

Facility For The Biological Treatment Of A Complex Chlorophenolic Waste - A Preliminary Report, William F. Evans

Journal of the Arkansas Academy of Science

The City of Jacksonville, Arkansas, is attempting to determine if aeration of a combined domestic sewage — chlorophenolic herbicide waste prior to release into conventional waste stabilization lagoons will be useful in the microbiological oxidation of chlorophenols and chlorophenoxy acids.


Growth Patterns In Bacillus Stearothermophilus, Harold B. Betton Jan 1969

Growth Patterns In Bacillus Stearothermophilus, Harold B. Betton

Journal of the Arkansas Academy of Science

No abstract provided.


Absence Of Pasteurella Pestis In The Wildlife Of Arkansas, R. Reece Corey, Leo J. Paulissen, Delbert Swartz Jan 1963

Absence Of Pasteurella Pestis In The Wildlife Of Arkansas, R. Reece Corey, Leo J. Paulissen, Delbert Swartz

Journal of the Arkansas Academy of Science

No abstract provided.


Further Studies On An Antibiotic Substance Produced By Rhizopus Nigricans Ehrenberg, Kenneth D. Mace, Delbert Swartz Jan 1963

Further Studies On An Antibiotic Substance Produced By Rhizopus Nigricans Ehrenberg, Kenneth D. Mace, Delbert Swartz

Journal of the Arkansas Academy of Science

No abstract provided.


Microorganisms And Sanitation In The Carbonated Beverage Industry, Kenneth D. Mace Jan 1955

Microorganisms And Sanitation In The Carbonated Beverage Industry, Kenneth D. Mace

Journal of the Arkansas Academy of Science

No abstract provided.


Method Of Calculating Exposures For Photomicrographs, Harold M. Hefley Jan 1951

Method Of Calculating Exposures For Photomicrographs, Harold M. Hefley

Journal of the Arkansas Academy of Science

No abstract provided.