Microbiology Commons^™

Cryptosporidiosis In The Immunosuppressed Mouse And In Cell Culture, Mercy Prabhu Das

Loma Linda University Electronic Theses, Dissertations & Projects

Cryptosporidiosis is a parasitic infection primarily of animals that has been recently recognized as an important disease in immunocompromised humans. Most cases to date have occurred in patients with acquired immunodeficiency syndrome (AIDS). Persons with abnormal humoral or cellular immune function, and even normal subjects, have also been affected.

To determine whether or not mice, when experimentally immunosuppressed, would develop cryptosporidiosis, a test was designed using the AKR/J strain. This strain was selected because, it was previously reported that it was the strain shown to respond most readily to the production of disease caused by the opportunistic protozoan, Pneumocystis carinii …

A Study Of Transferable Antibiotic Resistance In Enteric Bacteria, Charles H. Regeski

Loma Linda University Electronic Theses, Dissertations & Projects

A group of 48 enteric bacteria was selected from a total of 170 at Loma Linda University Medical Center because of resistance to three or more antibiotics. They were examined to see if any of the antibiotic resistances were transferred by conjugation to either of two recipient bacteria. These were a restrictionless, antibiotic sensitive, azide resistant mutant of Escherichia coli K 12 designated E. coli 1228 and a similar phenotypic strain of Salmonella typhimurium LT2 designated LB 5000. Forty-three transferred one or more antibiotic resistances to either or both recipients, a frequency of 25.3% of the total bacteria and 89.6% …

Lectins, El-Nasir Lalani, M Wells

Department of Pathology and Laboratory Medicine

No abstract provided.

Two Separable Functional Domains Of Simian Virus 40 Large T Antigen: Carboxyl-Terminal Region Of Simian Virus 40 Large T Antigen Is Required For Efficient Capsid Protein Synthesis., Joanne Tornow, Maryellen Polvino-Bodnar, George Santangelo, Charles N. Cole

Dartmouth Scholarship

The carboxyl-terminal portion of simian virus 40 large T antigen is essential for productive infection of CV-1 and CV-1p green monkey kidney cells. Mutant dlA2459, lacking 14 base pairs at 0.193 map units, was positive for viral DNA replication, but unable to form plaques in CV-1p cells (J. Tornow and C.N. Cole, J. Virol. 47:487-494, 1983). In this report, the defect of dlA2459 is further defined. Simian virus 40 late mRNAs were transcribed, polyadenylated, spliced, and transported in dlA2459-infected cells, but the level of capsid proteins produced in infected CV-1 green monkey kidney cells was extremely low. dlA2459 large T …

Dispelling Some Myths About 'Dermo', J R. Edwards

Journal of the Department of Agriculture, Western Australia, Series 4

There are a lot of misconceptions and myths about dermatophilosis, a disease which can kill young lambs, affect wool quality, make shearing difficult and make sheep susceptible to fly strike.

One misconception is perpetuated by the incorrect use of the name mycotic dermatitis, which implies that a fungus causes the disease. This is not so.

The disease is caused by the bacteria Dermatophilus congolensis, which attacks the sheep's skin and eventually forms a lesion or scab in the wool, and so is better called dermatophilosis, 'dermo' or 'lumpy wool'.

Veterinary Epidemiologist at the Albany Regional Office, J.R. Edwards, discusses …

New Developments In Footrot Control, R B. Richards, L. J. Depiazzi, R. V. R. Gwynn

Journal of the Department of Agriculture, Western Australia, Series 4

The eradication policy for footrot in sheep adopted by the Western Australian Department of Agriculture has produced an overall decline in the number of properties with the disease.

Our knowledge of the disease has improved substantially as a result of the research conducted at the Slabany Regional Veterinary Laboratory in recent years. Laboratory tests now sssist stock inspectors and veterinary officers to distinguish between different forms of footrot and to quarantine and eradicate accordingly.

The recent discovery of more effective foot-bathing solutions by officers of the Victorian Department of Agriculture will greatly assist further eradication procedures.

Western Australia's sheep industry …

The Voluntary Water Quality Monitoring Program : 1982 Project Report, Barbara Welch, Judy Potvin, Mary Ellen Dennis

Maine Collection

The Voluntary Water Quality Monitoring Program : 1982 Project Report

by Barbara Welch, Judy Potvin & Mary Ellen Dennis

Division of Environmental Evaluation and Lake Studies, Maine Department of Environmental Protection, Augusta, Maine 1982.

Contents: Understanding Maine's Lakes and Ponds / Acknowledgement / Temperature / Dissolved Oxygen / Other Measures of Lake Water Quality / Nutrients / Algae and Chlorophyll a / Transparency / Morphometry / Sampling / Protection / Classification / Answers to Most Frequently Asked Questions / Glossary of Terms / Appendix A : Maximum, Minimum, and Mean Values / Appendix B : Phosphate Content of Common Cleansers …

Petroleum-Utilizing Bacteria: The Biological Line Defense Against Oil Pollution, Steven W. Sokolowski

Biological Sciences Theses & Dissertations

A silica gel-petroleum (SGP) medium was developed which is reliable, approximately the same cost as that of the corresponding agar-based petroleum medium, and can be made sufficiently firm to use in streaking or the spread-plate technique. Advantages of the medium include: the incorporation of all of the major ions of seawater in accordance with Dittmar's Law (the salinity of which can easily be varied from 0 to 35 parts per thousand); a wide workable pH range (5.5 to 11.6); negligable pH drift(~ 0.2 pH units after 166 days); the elimination of all but negligable amounts of syneresis, the ability to …

Enzyme Linked Immunosorbent Assay For Anatid Herpesvirus, Margaret Anne Lewis

Biological Sciences Theses & Dissertations

Duck plague virus causes a hemorrhagic disease affecting the family Anatidae, resulting in 60-80% mortality in captive flocks. Plaque and neutralization assays for Anatid herpesvirus (AHV) require 5-10 days for completion and are restricted by the seasonal availability of fertile duck eggs. The enzyme linked innnunosorbent assay (ELISA) is rapid and specific and avoids the use of living cells. The Holland strain of AHV was grown on duck embryo fibroblasts and purified by banding on CsCl gradients. The resulting virus was used to raise antisera in prebled New Zealand white male rabbits. Antibody production was confirmed in double diffusion plates …

Isolation And Enumeration Of Enteric Viruses From Primary Wastewater Sludge, Michael Wayne Nath

Biological Sciences Theses & Dissertations

The presence of large numbers of enteric viruses in wastewaters has resulted in concern regarding their significance and consequences for humans. Assessments of relative significance or consequences may most accurately be made following precise measurements of the range of virus types and amounts in wastewaters and sludges and after epidemiological considerations. In this study, results are presented pertaining to enteric virus isolation, concentration, and enumeration from primary wastewater sludges.

As an integral part of a comparison of isolation procedures, several anionic surfactants were examined for extraction of enteric viruses from primary wastewater sludges. Plaquing efficiencies for poliovirus type 2 and …

The Development Of Techniques For The Enumeration Of Selected Pathogenic Bacteria In Lynnhaven Estuarine Sediments, Mark T. Frank

Biological Sciences Theses & Dissertations

Methodologies for the improved recovery of selected pathogenic bacteria from estuarine sediments were investigated. Laboratory and field studies were conducted to document the sensitivity of the techniques for isolating Salmonella species, Pseudomonas aeruginosa, and Vibrio parahaemolyticus. Vibrio parahaemolyticus-like organisms were enriched in a seawater yeast extract broth and isolated from Thiosulfate Citrate Bile Salts Sucrose plated media. Enrichment procedures with and without elevated temperatures and subsequent transfers to selective and differential plated media have been examined for the isolation of Salmonella species and P. aeruginosa. These pathogens were initially assayed for utilizing primary enrichments of asparagine …

Cell Mediated Immunity By Cytotoxicity Assay And The Effect Of Corynebacterium Parvum And Radiation On Mice Bearing Herpes-Induced Tumors, Robert B. Stagg

Loma Linda University Electronic Theses, Dissertations & Projects

Cell mediated immune (CMI) cytotoxic reactivity of Balb/c mice against H238 cells, a Herpes simplex virus (HSV) Type 2 - induced sarcoma, was measured by the ¹²⁵IUdR release assay. The Balb/c mouse response to the growing HSV-induced tumor, treated with radiation and , was measured by survival rate, tumor growth and immune cytotoxicity of spleen and peritoneal exudate cells (PEC) as determined by ¹²⁵IUdR release assay. Subcutaneous (s.c.) inoculation of 1 x 10⁶ H238 cells (high dose) produced progressive tumor growth while s.c. inoculation of 1 x 10⁴ H238 cells (low dose) produced no tumors. A …

Effects Of Trypanosoma Musculi Kendall, 1906 Immune Serum On The Reproduction Of Trichinella Spiralis (Owen, 1835) Railliet, 1896 In Mice, Samuel L. Chafin

Loma Linda University Electronic Theses, Dissertations & Projects

The possible effects of ablastin, a reproduction-inhibiting antibody which is produced during the infection of the blood parasite, Trypanosoma musculi in the mouse, were investigated by fractionating the parasite into a supernatant fraction (designated as Group A test mice) and a pellet fraction (Group B test mice). These groups A and B mice were injected with the respective fractions, plus Freund’s Complete Adjuvant (FCA). Control mice were injected with FCA only. Sera from these three groups of mice were removed after the Group A and Group B mice were shown to be negative to T. musculi challenge, and Group A …

Effect Of Time And Diet On The Immune Status Of Mice Challenged With Herpes-Transformed Cells, Daila S. Gridley

Loma Linda University Electronic Theses, Dissertations & Projects

Human Herpes simplex virus type 2 (HSV 2)-transformed Balb/c mouse cells (H238 cells) are malignant in Balb/c mice. The immune stratus of mice bearing H238 cell-induced tumors was studied and changes in immune response with time and with altered diet of the mice were measured.

In the experiments dealing with the status of immunity with time after tumor cell injection, mice were fed Purina Laboratory Chow (P.L.C.), injected with H238 cells, and sacrificed three to eight weeks post-injection. In the diet experiments, groups of mice were fed either P.L.C. or one of six equicaloric diets, consisting of low or high …

Demonstration Of Unique Mycelial Components In Candida Albicans, David Jon Mancuso

Biological Sciences Theses & Dissertations

Mycelial (M) and blastopore (B) cell homogenates of Candida albicans strain BJ85 were compared to detect and recover unique M components. Qualitative differences in the protein and lipid content of Mand B homogenates were suggested by discontinuous polyacrylamide gel electrophoresis (DPAGE). In addition, a quantitative and possibly qualitative shift in the protein content during germ tube formation was implied by DPAGE. Unique M components were detected using both DPAGE and immunoelectrophoretic (IE) techniques. One unique M component by DPAGE, four by IE, and two by combined DPAGE and IE techniques were suggested. Of these three methods, only DPAGE and the …

Lumpy Wool May Increase By Dipping : Research Roundup, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

Lumpy wool, or mycotic dermatitis, is an infectious disease on the skin of the sheep caused by the bacterium Dermatophilus conglensis. The organism invades the wool and hair follicles, causing formation of skin lesions producing scabby material which binds the wool fibres into a hard mass.

Research shows that 'dipping' may increase the incidence of lumpy wool with some chemicals a greater risk than others.

A Photomicrographic Guide To The Saprolegniaceae Of Illinois, Ronald L. Butler

Masters Theses

There is a paucity of information concerning the occurrence and distribution of the Saprolegniaceae of Illinois. These fungi, commonly called "water molds", are of particular interest as many are potential parasites of fish, crustaceans, and other aquatic plant and animal life. It is of considerable interest to students of the fungi and of practical value to the fisheries biologists and environmentalists to have available a means of identification for these organisms.

This paper provides a descriptive and photomicrographic account of the Saprolegniaceae of Illinois. Included herein are dichotomous keys to the genera of this family as well as generic descriptions …

Metabolic Capabilities Of Sulfur Oxidizing Bacteria And Their Role In Water Pollution, M. I. H. Aleem

KWRRI Research Reports

This report describes investigations into the physiology of microorganisms that are commonly involved in the oxidation of inorganic sulfur compounds. The metabolic activities of these bacteria play a potent role in several fields of economic importance such as strip mining operations, water pollution, corrosion, metallurgy, petroleum technology and soil fertility processes.

The oxidation of inorganic sulfur compounds was studied in the chemolithotrophs Thiobacillus denitrificans, Thiobacillus A₂, Thiobacillus neapolitanus, and a photolithotroph Rhodopseudomonas palustris. Cell suspensions from all of the thiobacilli catalyzed completely the oxidation of sulfide, thiosulfate, and sulfite to sulfate. The oxidation of thiosulfate in R. palustris …

The Deoxythymidine Kinase-Deoxythymidylate Kinase Enzymes In Salmonella Potsdam And Escherichia Coli K12 Infected With Bacteriophage P3, James D. Kettering

Loma Linda University Electronic Theses, Dissertations & Projects

Metabolic alterations in bacterial hosts caused by virus infections have been studied for the last thirty years. The virulent T-even bacteriophages have been studied the most and have been shown to be responsible for the induction of a number of new, virus-specific enzymes that are necessary for successful phage growth. In addition, the activity of other host enzymes present prior to infection has been found to be increased by T-even infection. Temperate bacteriophages, being more difficult to work with than the virulent viruses, have not been studied as extensively. This is especially true for metabolic studies, since most temperate phage …

Phytoplankton Succession In The Lafayette River, Estuary, Norfolk, Virginia, Thomas W. Purcell Lll

Biological Sciences Theses & Dissertations

Abstract unavailable.

Role Of Membrane-Associated Components Of Mycoplasma Arthritidis On The Biosynthesis Of Humoral Antibodies And Arthritogenic Properties In Experimental Animals, Lois M. Bergquist

Loma Linda University Electronic Theses, Dissertations & Projects

The virulence of animal-passaged Mycoplasma arthritidis (158) was demonstrated to be enhanced in rats by growth in oleic acid-enriched broth. The virulence of a laboratory-maintained culture of the same strain was unaffected by similar growth conditions. No differences either in the immune response as measured by complement-fixing (CF) antibodies or in the histogenesis of the disease were detected when rats were injected with animal-passaged M. arthritidis grown either in the presence or in the absence of oleic acid. All tests for metabolism-inhibiting (MI) antibodies in the rat were negative.

A Mycoplasma-associated immunosuppression of the primary and of the secondary hemagglutinin …

The Role Of D-Gluconic Acid In The Regulation Of The Synthesis Of The Enzymes Of The Entner-Doudoroff Pathway In Pseudomonas Fluorescens, Steven C. Quay

Honors Theses

Induction of Entner-Duodoroff pathway enzymes in Pseudomonas fluorescens was investigated to study the role of gluconate as a possible inducer. Glucose oxidase-deficient mutants were isolated and characterized. One of these mutants, gox-7, was deficient in particulate glucose oxidase; another mutant, gox-17, was deficient in particulate glucose and gluconate oxidase activities. Gluconate, but not glucose, induced synthesis of gluconokinase and 6-phosphogluconate dehydratase in both mutants. High constitute levels of 2-keto-3-deoxy-6-phosphogluconate aldolase were found when both mutants were grown on glucose. Growth of parent and both mutant strains on glycerol also resulted in high levels of Entner-Doudoroff pathway enzymes. It was concluded …

Bacteriuria As A Precursor Of Intracatheter Encrustation, Namcy Moll

Loma Linda University Electronic Theses, Dissertations & Projects

The problem with which this study was concerned was the development of criteria that could be used to determine when urinary catheter changes were necessary. By determining when encrustation occurred, it was felt that some complications could be eliminated by changing catheters before obstruction occurred due to encrustation.

It was the purpose of this study to: 1) explore some indications for catheter change, 2) determine if encrustation of urinary catheters necessitates catheter change, and 3) estimate potential criteria for a routine time for catheter changes.

Forty-five catheters were examined after their removal from patients. Flow rates were determined on 33 …

The Effect Of Elevated Glucose On Neutrophilic Phagocytosis In Genetically Diabetic Mice, Larry B. Thompson

Loma Linda University Electronic Theses, Dissertations & Projects

Infiltration to an injury site or area of bacterial contamination by polymorphonuclear leucocytes (or neutrophils) is an early and essential phase of the early inflammatory reaction. Their action on foreign matter is the most critical step taken by body defense mechanisms in the first 24 hours. Anything which interferes with that action, phagocytosis, greatly hampers early inflammation. This study was designed to see the effect of elevated glucose levels on neutrophil phagocytosis in a unique strain of genetically diabetic mice.

Twenty-two diabetic and twenty-four control female mice were used. Blood glucose determinations and total white counts were made on each …

Histopathological Variations Of Periapical Granulomas And Radicular Cysts, Thanapen Kanchanasut

Loma Linda University Electronic Theses, Dissertations & Projects

The periapical dental granuloma and cyst is the most frequent sequela of pulpitis. These lesions are always intraosseous and often become quiescent after the acute phases are over, forming a space which primarily consists of chronic inflammatory tissue. This chronic inflammatory tissue usually appears as a one-centimeter area of punched out bone on the x-ray film and is referred to as a dental granuloma. Should epithelium choose to proliferate in this inflammatory area, it may undergo subsequent cystic degeneration forming the typical periapical cyst. While much has been written with regard to the etiology and treatment of the apical cyst …

The Effectiveness Of Camphorated Paramonochlorophenol, Metacresyl Acetate, And Prednisolone In Combination As An Antimicrobial Agent, Maurice Cutler

Loma Linda University Electronic Theses, Dissertations & Projects

Post-treatment discomfort following endodontic procedures can be caused by inflammation due to injudicious use of intracanal medicaments. Camphorated paramonochlorophenol, metacresyl acetate, and prednisolone in combination is advocated to reduce the inflammatory potential of the germicide and thus reduce the occurrence of post-operative pain. It is imperative that a medicament retain antimicrobial activity while reducing inflammatory potential.

This investigation was undertaken toward determining on an in vitro quantitative bactericidal versus bacteriostatic basis by use of the broth dilution test tube method whether camphorated paramonochlorophenol, metacresyl acetate, and prednisolone in combination retains antimicrobial activity comparable to selected other commonly used nonspecific germicides. …

The Comparative Morphology Of The Female Gonads Of Two Members Of The Nematode Order Tylenchida, Owen G. Coker

Masters Theses

No abstract provided.

Can Mastitis Be Eliminated?, F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

Can mastitis be eliminated from a dairy herd? The results of a 12-month continuous study of four commercial herds show that it could be done.

Swine Fever And African Swine Fever, V W. Smith

Journal of the Department of Agriculture, Western Australia, Series 4

PIGS may become infected by viruses which also cause disease in other animals (for instance foot-and-mouth disease) but there are two virus diseases worthy of special mention because they cause clinical disease in pigs only.

A Survey Of The Incidence Of Salmonellosis In Three Groups Of Wild Animals, James T. Heiberger

Masters Theses

No abstract provided.