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Environmental Microbiology and Microbial Ecology

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Articles 601 - 630 of 1216

Full-Text Articles in Microbiology

Characterization Of Bacterial Communities In Biscayne Bay Through Genomic Analysis, Eric Fortman Dec 2019

Characterization Of Bacterial Communities In Biscayne Bay Through Genomic Analysis, Eric Fortman

HCNSO Student Theses and Dissertations

Biscayne Bay is a shallow oligotrophic estuary in Southeast Florida. Channelization of rivers, and dredging of canals has greatly altered the historical flow of fresh water into the bay. This, coupled with the rise of a sprawling urban & suburban development, has greatly increased the nutrient load in the bay. This study examined the bacterial community at 14 stations throughout Biscayne Bay —6 stations were located at the mouths of canals; 1 upstream-canal station; 6 stations in the center of the bay; and one ocean influenced station, located near the entrance to the bay. One liter, surface water samples were …


Use Of Uav Imagery And Nutrient Analyses For Estimation Of The Spatial And Temporal Contributions Of Cattle Dung To Nutrient Cycling In Grazed Ecosystems, Amanda E. Shine Dec 2019

Use Of Uav Imagery And Nutrient Analyses For Estimation Of The Spatial And Temporal Contributions Of Cattle Dung To Nutrient Cycling In Grazed Ecosystems, Amanda E. Shine

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Nutrient inputs from cattle dung are crucial drivers of nutrient cycling processes in grazed ecosystems. These inputs are important both spatially and temporally and are affected by variables such as grazing strategy, water location, and the nutritional profile of forage being grazed. Past research has attempted to map dung deposition patterns in order to more accurately estimate nutrient input, but the large spatial extent of a typical pasture and the tedious nature of identifying and mapping individual dung pats has prohibited the development of a time- and cost-effective methodology. The first objective of this research was to develop and validate …


The Effect Of Historic Shipwrecks On Sediment Microbiomes In The Northern Gulf Of Mexico, Melissa Brock Dec 2019

The Effect Of Historic Shipwrecks On Sediment Microbiomes In The Northern Gulf Of Mexico, Melissa Brock

Master's Theses

Microorganisms are ubiquitously distributed across all aquatic habitats, but it is the environmental conditions of a habitat that determine which microorganisms can thrive in terms of abundance or metabolic activity. Habitats that experience consistent physical and chemical environmental conditions repeatedly favor specific taxonomic groups which may result in a microbial assemblage that is commonly associated with that habitat (i.e., a core microbiome). Core microbiomes have been identified for a variety of natural marine habitats including methane seeps, wood falls, octocoral, and deep-sea sediments. However, it was unknown if the presence of man-made structures on the seafloor, including historic shipwrecks, also …


Species Richness And Ecological Diversity Of Myxomycetes And Myxomycete-Like Organisms In The Tropical Forests Of Brazil, Isadora Lima Coelho Dec 2019

Species Richness And Ecological Diversity Of Myxomycetes And Myxomycete-Like Organisms In The Tropical Forests Of Brazil, Isadora Lima Coelho

Graduate Theses and Dissertations

Tropical rain forests cover less than two percent of Earth's surface, yet they sustain the greatest diversity of living organisms on the planet. Tropical rain forests cover nearly 73% of the Brazilian territory and besides harboring some of the most biodiverse ecosystems on the planet, this vast area also houses about 83% of the Brazilian population. Approximately 175 million people live in urban and rural areas with fragments of coverage of these biomes which contributes to the loss of biodiversity that rapidly increases over the years. Furthermore, the majority of the taxonomic and ecological efforts to describe and protect the …


Evaluating Volatile Organic Compounds For Contact-Independent Antagonism Of Pseudogymnoascus Destructans Nov 2019

Evaluating Volatile Organic Compounds For Contact-Independent Antagonism Of Pseudogymnoascus Destructans

Symposium of Student Scholars

White-nose syndrome (WNS), a disease caused by the fungus Pseudogymnoascus destructans, is responsible for the extensive mortality of bats in the United States. In an effort to develop tools to reduce bat mortality attributed to WNS, an in vitro experiment was conducted to quantify the inhibitory effects of select volatile organic compounds (VOCs) as well as explore potential synergistic activities. The experiment involved exposing mycelial plugs of P. destructans to various concentrations of B23 as well as B23 and decanal together at equimolar ratios. Measurements of the plugs were taken over the course of the 13 day experiment allowing …


Effect Of Biosurfactant And Biochar On Ecosystem Functions And Oil Remediation In Crude Oil Contaminated Wetland Soils, Zhuo Wei Nov 2019

Effect Of Biosurfactant And Biochar On Ecosystem Functions And Oil Remediation In Crude Oil Contaminated Wetland Soils, Zhuo Wei

LSU Doctoral Dissertations

Decontamination of oil spill from coastal wetland soils requires a delicate approach. Remediation strategies using biochar, biosurfactant, and nutrient additions have been proposed. However, there is little information on application of these with combination for treatment of crude oil-contaminated wetland soil. Therefore, incubation studies were carried out to investigate the impact of biochar and rhamnolipid (RL) biosurfactant along with nitrogen (N) application on petroleum hydrocarbon degradation in a Louisiana coastal saline marsh and their impacts on greenhouse gas (GHG) emission. Additionally, the toxicity of crude oil as well as remediation materials on coastal wetland ecosystem were assessed at three levels: …


Inferred Function And Dynamics Of Microbial Communities From The Northern Gulf Of Mexico, Deepesh Tourani Nov 2019

Inferred Function And Dynamics Of Microbial Communities From The Northern Gulf Of Mexico, Deepesh Tourani

HCNSO Student Theses and Dissertations

Microbial communities, or microbiomes, are the major drivers of global biogeochemical cycles, acting as primary producers and decomposers across the water column in the oceans. Thus, they reflect changes in physicochemical properties and nutrient composition of the ocean. However, this correlation between ecological changes and the function of marine microbiomes is poorly understood. Large-scale oceanic events such as the bottom-water oxygen-depleted zone (i.e., “dead zone”) and the Deepwater Horizon oil spill in the Gulf of Mexico (GoM) render the ecosystem fragile. These events decrease survival rates of pelagic and coastal macrofauna and affect the biodiversity of the region. As part …


Morphology, Molecular Characterization And Habitat Preference Of Harmful Benthic Dinoflagellate Gambierdiscus (Dinophyceae) In Malaysia, Nurin Izzati Mustapa Nov 2019

Morphology, Molecular Characterization And Habitat Preference Of Harmful Benthic Dinoflagellate Gambierdiscus (Dinophyceae) In Malaysia, Nurin Izzati Mustapa

Student Works (2020-2029)

Ciguatera fish poisoning (CFP) is a type of illness that causes human intoxication after consuming contaminated coral fishes. The neurotoxins, ciguatoxins, are produced by species of Gambierdiscus, one of the harmful benthic dinoflagellates. Identification of harmful dinoflagellate species is important to gain further insights into the global dispersion. In this study, morphological characteristics of species in the genus Gambierdiscus and their habitat preferences were investigated. Field sampling was undertaken at Pulau Rawa, Terengganu and Pulau Sibu, Johor. Single cells of Gambierdiscus were isolated and clonal cultures were established. Morphology of Gambierdiscus was examined by using light and scanning electron microscopes. …


Identifiying High Risk Areas Of Zika Virus Infection By Meteorological Factors In Columbia, Lung-Chang Chien, Francisco Sy, Adriana Perez Oct 2019

Identifiying High Risk Areas Of Zika Virus Infection By Meteorological Factors In Columbia, Lung-Chang Chien, Francisco Sy, Adriana Perez

Environmental & Global Health Faculty Research

Background Several Zika virus (ZIKV) outbreaks have occurred since October 2015. Because there is no effective treatment for ZIKV infection, developing an effective surveillance and warning system is currently a high priority to prevent ZIKV infection. Despite Aedes mosquitos having been known to spread ZIKV, the calculation approach is diverse, and only applied to local areas. This study used meteorological measurements to monitor ZIKV infection due to the high correlation between climate change and Aedes mosquitos and the convenience to obtain meteorological data from weather monitoring stations. Methods This study applied the Bayesian structured additive regression modeling approach to include …


Insights Into The Ecological Roles And Evolution Of Methyl-Coenzyme M Reductase-Containing Hot Spring Archaea, Zheng-Shuang Hua, Yu-Lin Wang, Paul N. Evans, Yan-Ni Qu, Kian Mau Goh, Yang-Zhi Rao, Sun Yat-Sen University Qi, Yu-Xian Li, Min-Jun Huang, Jian-Yu Jiao, Ya-Ting Chen, Wen-Sheng Shu, Wael Hozzein, Brian P. Hedlund, Gene W. Tyson, Tong Zhang, Wen-Jun Li Oct 2019

Insights Into The Ecological Roles And Evolution Of Methyl-Coenzyme M Reductase-Containing Hot Spring Archaea, Zheng-Shuang Hua, Yu-Lin Wang, Paul N. Evans, Yan-Ni Qu, Kian Mau Goh, Yang-Zhi Rao, Sun Yat-Sen University Qi, Yu-Xian Li, Min-Jun Huang, Jian-Yu Jiao, Ya-Ting Chen, Wen-Sheng Shu, Wael Hozzein, Brian P. Hedlund, Gene W. Tyson, Tong Zhang, Wen-Jun Li

Life Sciences Faculty Research

Several recent studies have shown the presence of genes for the key enzyme associated with archaeal methane/alkane metabolism, methyl-coenzyme M reductase (Mcr), in metagenome-assembled genomes (MAGs) divergent to existing archaeal lineages. Here, we study the mcr-containing archaeal MAGs from several hot springs, which reveal further expansion in the diversity of archaeal organisms performing methane/alkane metabolism. Significantly, an MAG basal to organisms from the phylum Thaumarchaeota that contains mcr genes, but not those for ammonia oxidation or aerobic metabolism, is identified. Together, our phylogenetic analyses and ancestral state reconstructions suggest a mostly vertical evolution of mcrABG genes among methanogens and methanotrophs, …


Insights Into The Ecological Roles And Evolution Of Methyl-Coenzyme M Reductase-Containing Hot Spring Archaea, Zheng-Shuang Hua, Yu-Lin Wang, Paul N. Evans, Yan-Ni Qu, Kian Mau Goh, Yang-Zhi Rao, Yan-Ling Qi, Yu-Xian Li, Min-Jun Huang, Jian-Yu Jiao, Ya-Ting Chen, Wen-Sheng Shu, Wael Hozzein, Brian P. Hedlund, Gene W. Tyson, Tong Zhang, Wen-Jun Li Oct 2019

Insights Into The Ecological Roles And Evolution Of Methyl-Coenzyme M Reductase-Containing Hot Spring Archaea, Zheng-Shuang Hua, Yu-Lin Wang, Paul N. Evans, Yan-Ni Qu, Kian Mau Goh, Yang-Zhi Rao, Yan-Ling Qi, Yu-Xian Li, Min-Jun Huang, Jian-Yu Jiao, Ya-Ting Chen, Wen-Sheng Shu, Wael Hozzein, Brian P. Hedlund, Gene W. Tyson, Tong Zhang, Wen-Jun Li

Life Sciences Faculty Research

Several recent studies have shown the presence of genes for the key enzyme associated with archaeal methane/alkane metabolism, methyl-coenzyme M reductase (Mcr), in metagenome-assembled genomes (MAGs) divergent to existing archaeal lineages. Here, we study the mcr-containing archaeal MAGs from several hot springs, which reveal further expansion in the diversity of archaeal organisms performing methane/alkane metabolism. Significantly, an MAG basal to organisms from the phylum Thaumarchaeota that contains mcr genes, but not those for ammonia oxidation or aerobic metabolism, is identified. Together, our phylogenetic analyses and ancestral state reconstructions suggest a mostly vertical evolution of mcrABG genes among methanogens and methanotrophs, …


Simulating Bacterial Growth, Competition, And Resistance With Agent-Based Models And Laboratory Experiments, Anne E. Yust, Davida S. Smyth Oct 2019

Simulating Bacterial Growth, Competition, And Resistance With Agent-Based Models And Laboratory Experiments, Anne E. Yust, Davida S. Smyth

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.


Reproduction And Dispersal Of Biological Soil Crust Organisms, Steven D. Warren, Larry L. St. Clair, Lloyd R. Stark, Louise A. Lewis, Nuttapon Pombubpa, Tania Kurbessoian, Jason E. Stajich, Zachary T. Aanderud Oct 2019

Reproduction And Dispersal Of Biological Soil Crust Organisms, Steven D. Warren, Larry L. St. Clair, Lloyd R. Stark, Louise A. Lewis, Nuttapon Pombubpa, Tania Kurbessoian, Jason E. Stajich, Zachary T. Aanderud

Life Sciences Faculty Research

Biological soil crusts (BSCs) consist of a diverse and highly integrated community of organisms that effectively colonize and collectively stabilize soil surfaces. BSCs vary in terms of soil chemistry and texture as well as the environmental parameters that combine to support unique combinations of organisms—including cyanobacteria dominated, lichen-dominated, and bryophyte-dominated crusts. The list of organismal groups that make up BSC communities in various and unique combinations include—free living, lichenized, and mycorrhizal fungi, chemoheterotrophic bacteria, cyanobacteria, diazotrophic bacteria and archaea, eukaryotic algae, and bryophytes. The various BSC organismal groups demonstrate several common characteristics including—desiccation and extreme temperature tolerance, production of various …


Coffee Is Not Forever : A Global History Of The Coffee Leaf Rust, Stuart Mccook Oct 2019

Coffee Is Not Forever : A Global History Of The Coffee Leaf Rust, Stuart Mccook

Ohio University Press Open Access Books

The global coffee industry, which fuels the livelihoods of farmers, entrepreneurs, and consumers around the world, rests on fragile ecological foundations. In Coffee Is Not Forever, Stuart McCook explores the transnational story of this essential crop through a history of one of its most devastating diseases, the coffee leaf rust. He deftly synthesizes agricultural, social, and economic histories with plant genetics and plant pathology to investigate the increasing interdependence of the world’s coffee-producing zones. In the process, he illuminates the progress and prognosis of the challenges—especially climate change—that pose an existential threat to a crop that global consumers often take …


What About The Environment?: Exploring The Neglected Third Dimension Of Antimicrobial Resistance, Paige E. Montfort Oct 2019

What About The Environment?: Exploring The Neglected Third Dimension Of Antimicrobial Resistance, Paige E. Montfort

Independent Study Project (ISP) Collection

Antimicrobial resistance (AMR) is one of the most urgent and complex health risks of our time, with links to human health, animal health, and the environment. The majority of research and policy related to AMR, however, has been dedicated to human and animal health. The third dimension — the environment — has been relatively neglected. Conversations about this problem have begun, but gaps in understanding remain. This study explores the key barriers that have hindered developments related to the environmental aspect of AMR and some of the solutions that have begun to or could be utilized to overcome these barriers. …


Tungsten Is Essential For Long-Term Maintenance Of Members Of Candidate Archaeal Genus Aigarchaeota Group 4, Joshua Robert Reyes Dimapilis Sep 2019

Tungsten Is Essential For Long-Term Maintenance Of Members Of Candidate Archaeal Genus Aigarchaeota Group 4, Joshua Robert Reyes Dimapilis

Electronic Theses, Projects, and Dissertations

Aigarchaeota, a deeply branching archaeal lineage with no cultivated representatives, is found in geothermal and hydrothermal systems worldwide and consists of at least 9 genus-level groups, each predicted to have diverse metabolic capabilities. This candidate archaeal phylum is part of the TACK superphylum, members of which possess eukaryotic-signature proteins, thus suggesting that they may represent evolutionary steps along the way to the genesis of the first eukaryotic cells. Cultivating members of Aigarchaeota would elucidate how eukaryotes arose in evolutionary history and provide biotechnological applications. Aigarchaeota Group 4 (AigG4), one genus in Aigarchaeota, was previously found to be abundant in corn …


Isolation Of Caldatribacterium (Op9) And Investigation Of Its Potential Interactions With A Novel, Co-Cultivated Thermodesulfobacterium Species, Toshio Alvarado Sep 2019

Isolation Of Caldatribacterium (Op9) And Investigation Of Its Potential Interactions With A Novel, Co-Cultivated Thermodesulfobacterium Species, Toshio Alvarado

Electronic Theses, Projects, and Dissertations

Atribacteria (OP9), candidate phylum with no representatives in pure culture, is found in various anaerobic environments worldwide. “Caldatribacterium”, a lineage within Atribacteria that is predicted to be a strictly anaerobic sugar fermenter based on cultivation-independent genomic analyses, is currently being maintained in lab enrichment cultures with fucose as its sole growth substrate. Metagenomics and 16S rRNA gene tag sequencing indicated that the fucose culture was a co-culture of “Caldatribacterium” and an uncultivated member of the genus Thermodesulfobacterium. Due to failed attempts to isolate “Caldatribacterium” by dilution-to-extinction and plating, it was hypothesized that “Caldatribacterium” is dependent in some way on the …


Using Single-Cell Sorting, Fish And 13c-Labeling To Cultivate And Assess Carbon Substrate Utilization Of ‘Aigarchaeota’ And Other Novel Thermophiles, Damon Kurtis Mosier Sep 2019

Using Single-Cell Sorting, Fish And 13c-Labeling To Cultivate And Assess Carbon Substrate Utilization Of ‘Aigarchaeota’ And Other Novel Thermophiles, Damon Kurtis Mosier

Electronic Theses, Projects, and Dissertations

‘Aigarchaeota’, a deeply branching lineage in the domain Archaea with no cultivated representatives, includes both thermophilic and hyperthermophilic microorganisms that reside in terrestrial and marine geothermal environments. The ‘Aigarchaeota’ consists of at least nine proposed genus-level groups that have been confirmed via 16S rRNA sequencing, with ‘Aigarchaeota’ Group 1 (AigG1) being the focus of this study. Based on cultivation-independent genomic data available from several AigG1 members in Great Boiling Spring (GBS), NV, and Yellowstone National Park, 22 different types of growth media were designed and tested for their ability to support growth of AigG1. One of these cultures, G1-10, was …


Are Microclimate Conditions In El Malpais National Monument Caves In New Mexico, Usa Suitable For Pseudogymnoascus Growth?, Terry J. Torres-Cruz, Andrea Porras-Alfaro, Nicole A. Caimi, Ogochukwu Nwabologu, Edward W. Strach, Kaitlyn J.H. Read, Jesse M. Young, Debbie C. Buecher, Diana E. Northup Aug 2019

Are Microclimate Conditions In El Malpais National Monument Caves In New Mexico, Usa Suitable For Pseudogymnoascus Growth?, Terry J. Torres-Cruz, Andrea Porras-Alfaro, Nicole A. Caimi, Ogochukwu Nwabologu, Edward W. Strach, Kaitlyn J.H. Read, Jesse M. Young, Debbie C. Buecher, Diana E. Northup

International Journal of Speleology

White-nose syndrome (WNS) is a bat disease caused by the fungal pathogen Pseudogymnoascus destructans, which thrives in cold and very humid environments where bats frequently hibernate. Conidia of Pseudogymnoascus species are often documented on bats prior to the onset of WNS, but characterization of high-risk areas defined by microclimate cave conditions have been lacking. Investigating the occurrence of this fungal genus and appropriate environmental conditions to support P. destructans in southwestern U.S. caves is key to understanding the sites most likely to be impacted by WNS. Microclimate conditions in ten caves at El Malpais (ELMA) National Monument in New …


Glycolic Acid Utilization In Two Species Of Marine Bacteria, Erik S. Timsak Aug 2019

Glycolic Acid Utilization In Two Species Of Marine Bacteria, Erik S. Timsak

STAR Program Research Presentations

Interactions between bacteria and phytoplankton are crucial for the cycling of organic matter in marine environments. Around 50% of organic carbon taken up by marine bacteria is converted into inorganic carbon. The uptake of organic carbon by marine bacteria exuded from phytoplankton is a key factor in regulating the marine carbon cycle. One such molecule that is exuded by phytoplankton and then uptaken by marine bacteria is called glycolate - the anion of glycolic acid, a two caron molecule. Glycolate is exuded by phytoplankton during photorespiration and 10-50% of dissolved organic carbon in marine environments is comprised of glycolate. Additionally, …


16s Rrna Analysis And Toxin Gene Presence In Escherichia Coli Isolated From Beach Water And Sand At A Public Beach (Erie County, Ny), Jennifer D. Jackson Aug 2019

16s Rrna Analysis And Toxin Gene Presence In Escherichia Coli Isolated From Beach Water And Sand At A Public Beach (Erie County, Ny), Jennifer D. Jackson

Biology Theses

Every year, thousands of people utilize beaches for recreation, but most are unaware of Escherichia coli (E. coli) contamination and the possibility of acquiring an infection. In this study, 173 strains of E. coli were isolated from sand and adjacent waters from a public beach in Erie County, NY and analyzed for genetic relatedness based on sequence differences in the variable regions of the 16S rRNA gene. Some of the variable regions (V1 and V6) proved useful in constructing phylogenetic trees but the discriminatory power of these regions was inadequate to resolve intraspecies differences. Therefore, whether extant populations …


Characterizing Nodule Endophyte Communities In Glycine Max And Lablab Purpureus Using Next-Generation Sequencing, Scott David Mcelveen Aug 2019

Characterizing Nodule Endophyte Communities In Glycine Max And Lablab Purpureus Using Next-Generation Sequencing, Scott David Mcelveen

Graduate Theses/Dissertations

Biological nitrogen fixation by rhizobia in the root nodules of legumes is a significant source of agricultural nitrogen in global crop production systems. The influence of and interaction of factors involved in nodule endophyte selection remain poorly understood. In the present study, the influences of crop rotation (soybean-legume vs. cotton-legume) and recalcitrant soil organic matter (compost amendment) on the relative distribution of endophytic bacteria in the root nodules of greenhouse-grown soybean and lablab were investigated by extracting, amplifying, and sequencing 16S ribosomal RNA (rRNA) and nifH genes. Neither preceding crop nor compost amendment were found to have an influence on …


Molecular Detection Of Pathogenic Leptospira Throughout The Caribbean, Zamara R. Garcia Truitt Jun 2019

Molecular Detection Of Pathogenic Leptospira Throughout The Caribbean, Zamara R. Garcia Truitt

Biology Theses

Leptospirosis is a common widespread zoonotic disease found throughout the Caribbean. The infection is produced by a pathogenic Leptospira species found in tropical and subtropical regions. The severity of the disease ranges from a mild asymptomatic infection to death. Leptospirosis is a neglected tropical disease due to the lack of public awareness and health approaches. Therefore, misdiagnosis is common because the symptoms are similar to that of other tropical endemic diseases. The aim of the study was to determine the presence of pathogenic Leptospira in water samples collected from Puerto Rico and Trinidad. Sixty-four environmental water samples were collected throughout …


Equivalency Of Indirect And Direct Potable Reuse Paradigms Based On A Quantitative Microbial Risk Assessment Framework, Erfaneh Amoueyan, Yongsheng He, Hongjie Wu, Chuanwei Zhu, Shichun Huang, Jian Huang Jun 2019

Equivalency Of Indirect And Direct Potable Reuse Paradigms Based On A Quantitative Microbial Risk Assessment Framework, Erfaneh Amoueyan, Yongsheng He, Hongjie Wu, Chuanwei Zhu, Shichun Huang, Jian Huang

Civil and Environmental Engineering and Construction Faculty Research

Due to rapid growth in the potable reuse industry, there is a need to better characterize the associated pathogen risks and degree of attenuation achieved by various treatment trains. It is also important to understand how emerging treatment frameworks compare to the historical practice of de facto reuse. As such, the goals of this study were to (1) evaluate the equivalency of indirect potable reuse (IPR) and direct potable reuse (DPR) systems; (2) compare alternative treatment trains; and (3) identify the design components and operational conditions that are most critical to minimizing public health risks. To this end, we developed …


Hawk On Wire: Ecopoems By Scott T. Starbuck, Vivian M. Hansen Jun 2019

Hawk On Wire: Ecopoems By Scott T. Starbuck, Vivian M. Hansen

The Goose

Review of Scott T. Starbuck’s Hawk on Wire: Ecopoems


Culture-Based Analysis Of ‘Cave Silver’ Biofilms On Rocks In The Former Homestake Mine In South Dakota, Usa, Amanpreet K. Brar, David Bergmann Jun 2019

Culture-Based Analysis Of ‘Cave Silver’ Biofilms On Rocks In The Former Homestake Mine In South Dakota, Usa, Amanpreet K. Brar, David Bergmann

International Journal of Speleology

Tunnels in a warm, humid area of the 1478 m level of the Sanford Underground Research Facility (SURF), located in a former gold mine in South Dakota, USA, host irregular, thin whitish, iridescent biofilms, which appear superficially similar to ‘cave silver’ biofilms described from limestone and lava tube caves, despite the higher rock temperature (32°C) and differing rock surface (phyllite) present at SURF. In this study, we investigated the diversity of cultivable bacteria constituting the cave silver by using several media: CN agar, CN gellan gum and 0.1X R2A agar. The highest colony count (CFU/g of sample) was observed on …


Investigation Of Origin And Growth Of Fungi In Coastal Beach Sand, Ruth L. Wright May 2019

Investigation Of Origin And Growth Of Fungi In Coastal Beach Sand, Ruth L. Wright

Honors Theses

In a previous study from this lab (Stevens, Evans, & Aguirre, 2012) yeast colonies were isolated and grown grown from sand collection at pristine, medium use and high use beaches along the grand strand. In that study greater yeast abundance and greater diversity both correlated with higher census of human use, suggesting that some yeast growth of sites was anthropogenic. However the sand matrix itself also varied from very fine, uniform, dark sand at the pristine beach to coarser, color varying, less tightly packed sand at residential and commercial beaches. This suggested an alternative hypothesis to differential colonization and growth …


See The Forest For The Leaves: Foliar Fungal Community Composition And Volatile Organic Compound Production, Joshua Copeland May 2019

See The Forest For The Leaves: Foliar Fungal Community Composition And Volatile Organic Compound Production, Joshua Copeland

Master's Theses

Endophytes are microbes that live within plants without causing detectable illness. These microorganisms can emit volatile organic compounds (VOCs), airborne signaling molecules that can significantly affect plant growth. Despite the prevalence of endophytes and their VOCs, little is known about these factors important to plant health. With this in mind I isolated fungal endophytes from leaves of Populus trichocarpa and Populus fremontii, tree species with economic and environmental uses, to determine if endophyte communities are reserved between the closely related tree species and over seasons. Surface sterilized leaf fragments were plated onto malt extract agar and the resulting fungi were …


The Effects Of Constructed Wetlands On Metal Solubilization And Bioavailability In Passive Mine Remediation, Elizabeth Cochran May 2019

The Effects Of Constructed Wetlands On Metal Solubilization And Bioavailability In Passive Mine Remediation, Elizabeth Cochran

Electronic Theses and Dissertations

The legacy of coal mining in Appalachia has left many watersheds impacted by abandoned mine drainage. The drainage degrades streams, groundwater and habitats with acidity and toxic heavy metals. The Lowber Passive Remediation System has been in operation since 2004 to remediate high amounts of dissolved Fe, SO4, Al, and Mn. The site consists of six settling ponds and an extensive wetlands at the finish. This study investigated the resolubilization of Fe and Mn in the wetlands and the role of bacteria in resolubilization. During three seasons, field measurements of water quality and lab analysis of Fe, Mn, …


Fungi In A Hot, Dry, Changing World, Miriam I. Hutchinson May 2019

Fungi In A Hot, Dry, Changing World, Miriam I. Hutchinson

Biology ETDs

My doctoral work focused on understanding the reciprocal relationship between fungi and their environment, namely how fungi respond to environmental flux, as well as how fungi can modify and structure their habitats, especially in the context of climate change. As such, I aimed my research on fungi with distinct adaptations to their environmental niches: endophytic fungi that inhabit plant tissue and thermophilic fungi that are capable of growing at the upper temperature limit for eukaryotic life. My research consisted of three studies. First, I investigated the thermophilic species Myceliophthora heterothallica to demonstrate its use as a model organism for efficient …