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Articles 31 - 60 of 113

Full-Text Articles in Laboratory and Basic Science Research

Rapid Golgi Stain For Dendritic Spine Visualization In Hippocampus And Prefrontal Cortex, Maya Frankfurt, Rachel E. Bowman Jan 2021

Rapid Golgi Stain For Dendritic Spine Visualization In Hippocampus And Prefrontal Cortex, Maya Frankfurt, Rachel E. Bowman

Psychology Faculty Publications

Golgi impregnation, using the Golgi staining kit with minor adaptations, is used to impregnate dendritic spines in the rat hippocampus and medial prefrontal cortex. This technique is a marked improvement over previous methods of Golgi impregnation because the premixed chemicals are safer to use, neurons are consistently well impregnated, there is far less background debris, and for a given region, there are extremely small deviations in spine density between experiments. Moreover, brains can be accumulated after a certain point and kept frozen until further processing. Using this method any brain region of interest can be studied. Once stained and cover …


Introduction To Biomedical Research (Temporary) Cmb 240x, Michael Cerbo Sep 2020

Introduction To Biomedical Research (Temporary) Cmb 240x, Michael Cerbo

Library Impact Statements

No abstract provided.


Geographical Information Systems In Python Nrs 528, Michael Cerbo Oct 2019

Geographical Information Systems In Python Nrs 528, Michael Cerbo

Library Impact Statements

No abstract provided.


A Belmont Report For Animals?, Hope Ferdowsian, L. Syd M. Johnson, Jane Johnson, Andrew Fenton, Adam Shriver, John Gluck Oct 2019

A Belmont Report For Animals?, Hope Ferdowsian, L. Syd M. Johnson, Jane Johnson, Andrew Fenton, Adam Shriver, John Gluck

Professional Science Research Ethics Collection

Human and animal research both operate within established standards. In the United States, criticism of the human research environment and recorded abuses of human research subjects served as the impetus for the establishment of the National Commission for the Protection of Human Subjects of Biomedical and Behavioral Research, and the resulting Belmont Report. The Belmont Report established key ethical principles to which human research should adhere: respect for autonomy, obligations to beneficence and justice, and special protections for vulnerable individuals and populations. While current guidelines appropriately aim to protect the individual interests of human participants in research, no similar, comprehensive, …


Animal Experimentation: Working Towards A Paradigm Change, Kathrin Herrmann (Ed.), Kimberley Jayne (Ed.) Apr 2019

Animal Experimentation: Working Towards A Paradigm Change, Kathrin Herrmann (Ed.), Kimberley Jayne (Ed.)

eBooks

Animal experimentation has been one of the most controversial areas of animal use, mainly due to the intentional harms inflicted upon animals for the sake of hoped-for benefits in humans. Despite this rationale for continued animal experimentation, shortcomings of this practice have become increasingly more apparent and well-documented. However, these limitations are not yet widely known or appreciated, and there is a danger that they may simply be ignored. The 51 experts who have contributed to Animal Experimentation: Working Towards a Paradigm Change critically review current animal use in science, present new and innovative non-animal approaches to address urgent scientific …


Laboratory Rotations In Cell And Molecular Biology, Michael Cerbo Apr 2019

Laboratory Rotations In Cell And Molecular Biology, Michael Cerbo

Library Impact Statements

No abstract provided.


Publication Reform To Safeguard Wildlife From Researcher Harm, Kate A. Field, Paul C. Paquet, Kyle A. Artelle, Gilbert Proulx, Ryan K. Brook, Chris T. Darimont Apr 2019

Publication Reform To Safeguard Wildlife From Researcher Harm, Kate A. Field, Paul C. Paquet, Kyle A. Artelle, Gilbert Proulx, Ryan K. Brook, Chris T. Darimont

Professional Science Research Ethics Collection

Despite abundant focus on responsible care of laboratory animals, we argue that inattention to the maltreatment of wildlife constitutes an ethical blind spot in contemporary animal research. We begin by reviewing significant shortcomings in legal and institutional oversight, arguing for the relatively rapid and transformational potential of editorial oversight at journals in preventing harm to vertebrates studied in the field and outside the direct supervision of institutions. Straightforward changes to animal care policies in journals, which our analysis of 206 journals suggests are either absent (34%), weak, incoherent, or neglected by researchers, could provide a practical, effective, and rapidly imposed …


Laboratory Rotations In Cell And Molecular Biology Cmb 503x, Michael Cerbo Apr 2019

Laboratory Rotations In Cell And Molecular Biology Cmb 503x, Michael Cerbo

Library Impact Statements

No abstract provided.


Refinement On The Way Towards Replacement: Are We Doing What We Can?, Kathrin Herrmann Jan 2019

Refinement On The Way Towards Replacement: Are We Doing What We Can?, Kathrin Herrmann

Humane Science Movement Collection

No abstract provided.


Contesting Animal Experiments Through Ethics And Epistemology: In Defense Of A Political Critique Of Animal Experimentation, Arianna Ferrari Jan 2019

Contesting Animal Experiments Through Ethics And Epistemology: In Defense Of A Political Critique Of Animal Experimentation, Arianna Ferrari

Professional Science Research Ethics Collection

Generally, an animal experiment can be defined as an intervention on an animal, which causes suffering, harm, and distress, for scientific purposes. In this definition, animal experiments differ from more general scientific investigations concerning animals, such as observational studies in the wild in the fields of ethology or conservation, in which animals are involved but may not be harmed. Nowadays, the use of the term vivisection, in the case of animal experiments, is very controversial. This term originally referred to the cutting of living bodies for scientific purposes and has a long conceptual history (Maehle, 1992). In ancient times, it …


Rethinking The 3rs: From Whitewashing To Rights, Charlotte E. Blattner Jan 2019

Rethinking The 3rs: From Whitewashing To Rights, Charlotte E. Blattner

Humane Science Movement Collection

Few other issues have prompted as many legislators to adopt legal instruction on the “proper” use of non-human animals (hereinafter referred to as animals) in medical and scientific research. Today, the 3Rs (replacement, reduction, and refinement of animals in scientific procedures) are globally accepted by a vast majority of states (Blattner, 2014); and prominent international organizations, such as the World Organisation for Animal Health (Terrestrial Animal Health Code, 2018, Article 7(8)(3)) and the Council of Europe (Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes, 1986, Articles 6(2), 7 and 8). Widespread acceptance of the …


When Is An Alternative Not An Alternative? Supporting Progress For Absolute Replacement Of Animals In Science, Craig Redmond Jan 2019

When Is An Alternative Not An Alternative? Supporting Progress For Absolute Replacement Of Animals In Science, Craig Redmond

Humane Science Movement Collection

This chapter addresses some of the areas in which animals are still used within “alternatives”-based research and calls on animal welfare and in vitro organizations to lead the debate and encourage absolute replacement of animal use in research. Without this, progress to end animal research will always remain limited, despite the paradigm shift seen in recent decades.


Research And Testing Without Animals: Where Are We Now And Where Are We Heading?, Thomas Hartung Jan 2019

Research And Testing Without Animals: Where Are We Now And Where Are We Heading?, Thomas Hartung

Humane Science Movement Collection

Experiments involving non-human animals (hereinafter referred to as animals) were the predominant technology in the life sciences from the 1920s to the 1970s. Increasingly, animal-based procedures have been complemented and superseded by other approaches; yet, they still have an enormous reputation as an apparent definitive answer to many scientific and, especially, regulatory questions. They have been questioned first for ethical reasons: Can we justify making animals suffer for scientific inquiry? Simply said, people have different views on this question, but the general public views animal experimentation more and more critically. The animal research community has sought a compromise between those …


Smart Sheep Need More Protection, Michael L. Woodruff Jan 2019

Smart Sheep Need More Protection, Michael L. Woodruff

Animal Sentience

The target article unequivocally establishes that sheep are far more intelligent and cognitively sophisticated than is generally acknowledged. For this reason, the authors advocate for significantly more stringent regulation of agricultural and research practices when sheep are used. I briefly review the existing US regulations governing the use of sheep in research and discuss the extent to which they are applied to sheep. I then discuss weaknesses in the current regulations, concluding that they should be changed to mandate housing all research animals in environments that accommodate the psychosocial needs of each species.


Environmental Health And Saafety For Scientists Bps 357x, Joanna Burkhardt Nov 2018

Environmental Health And Saafety For Scientists Bps 357x, Joanna Burkhardt

Library Impact Statements

No abstract provided.


Importance Of Welfare And Ethics Competence Regarding Animals Kept For Scientific Purposes To Veterinary Students In Australia And New Zealand, Teresa Collins, Amelia Cornish, Jennifer Hood, Chris Degeling, Andrew D. Fisher, Rafael Freire, Susan J. Hazel, Jane Johnson, Jennifer K.F. Lloyd, Clive J.C. Phillips, Vicky Tzioumis, Paul D. Mcgreevy Jul 2018

Importance Of Welfare And Ethics Competence Regarding Animals Kept For Scientific Purposes To Veterinary Students In Australia And New Zealand, Teresa Collins, Amelia Cornish, Jennifer Hood, Chris Degeling, Andrew D. Fisher, Rafael Freire, Susan J. Hazel, Jane Johnson, Jennifer K.F. Lloyd, Clive J.C. Phillips, Vicky Tzioumis, Paul D. Mcgreevy

Professional Science Research Ethics Collection

Veterinarians are in a strong position of social influence on animal-related issues. Hence, veterinary schools have an opportunity to raise animal health and welfare standards by improving veterinary students’ animal welfare and ethics (AWE) education, including that related to animals used for scientific purposes. A survey of 818 students in the early, mid, and senior stages of their courses at all eight veterinary schools across Australia and New Zealand was undertaken on their first day of practice (or Day One Competences) to explore how veterinary students viewed the importance of their competence in the management of welfare and ethical decision-making …


Literature Review And Proposal: Yoga As Group Exercise Involving Oxytocin Release For Positive Mood Improvement, Rachel Fenton May 2018

Literature Review And Proposal: Yoga As Group Exercise Involving Oxytocin Release For Positive Mood Improvement, Rachel Fenton

Honors Projects

A literature review discusses yoga and health involving oxytocin creates the premise for a proposal combining the knowledge of yoga and its health benefits along with oxytocin's potential involvement during group exercise, yoga specifically. The proposed study's results of oxytocin measures and questionnaires have the potential to develop an understanding of the possible impacts of yoga on mood, particularly relationships between group exercise and yoga, which may help develop forms of group exercise or implement group yoga to assist or replace treatment for stress-caused or stress-related disorders.


The Ush2a Gene: An Analysis Of Ultrasonic Vocalizations In A Mouse Model Of Usher Syndrome Type 2, Kiana R. Akhundzadeh May 2018

The Ush2a Gene: An Analysis Of Ultrasonic Vocalizations In A Mouse Model Of Usher Syndrome Type 2, Kiana R. Akhundzadeh

Honors Scholar Theses

Usher syndrome type 2 is a complex autosomal recessive genetic disorder that is characterized by moderate to severe congenital sensorineural hearing loss, the onset of retinitis pigmentosa in the second decade of life, and in some cases, vestibular dysfunction. Mutations in the USH2A gene account for 85% of cases of type 2. The USH2A gene is responsible for encoding the protein usherin, which has an important role in the development and function of inner ear hair cells and retinal photoreceptors. Until recently, it has been believed that carriers of the USH2A mutation were phenotype free. However, recent data has suggested …


Effect Of Metal Ion Substitution On Electrochemical Properties Of Cobalt Oxide For Energy Applications, Dalal Alqahtani Dec 2017

Effect Of Metal Ion Substitution On Electrochemical Properties Of Cobalt Oxide For Energy Applications, Dalal Alqahtani

Electronic Theses & Dissertations

Metal oxides are being used for various applications, such as batteries, supercapacitors, and catalysts. This research is mainly to produce high-performance energy storage devices capable of working at high temperatures and causing no pollution in the environment. To this end, the effect of metal ion substitution on the electrochemical properties of Co3O4 for energy applications was studied. Different metal oxides, such as FeCo2O4, MnCo2O4, Co3O4, NiCo2O4, CuCo2O4, and ZnCo2O4, were synthesized using a hydrothermal …


Advances In Neuroscience Imply That Harmful Experiments In Dogs Are Unethical, Jarrod Bailey, Shiranee Pereira Jul 2017

Advances In Neuroscience Imply That Harmful Experiments In Dogs Are Unethical, Jarrod Bailey, Shiranee Pereira

Professional Science Research Ethics Collection

Functional MRI (fMRI) of fully awake and unrestrained dog 'volunteers' has been proven an effective tool to understand the neural circuitry and functioning of the canine brain. Although every dog owner would vouch that dogs are perceptive, cognitive, intuitive and capable of positive emotions/empathy, as indeed substantiated by ethological studies for some time, neurological investigations now corroborate this. These studies show that there exists a striking similarity between dogs and humans in the functioning of the caudate nucleus (associated with pleasure and emotion), and dogs experience positive emotions, empathic-like responses and demonstrate human bonding which, some scientists claim, may be …


Elucidating The Effects Of Thiamethoxam Neonicotinoid On Honey Bee Learning Using The Proboscis Extension Response, David J. Shepherd May 2017

Elucidating The Effects Of Thiamethoxam Neonicotinoid On Honey Bee Learning Using The Proboscis Extension Response, David J. Shepherd

Undergraduate Honors Theses

In this study, the effects of the neonicotinoid pesticide, thiamethoxam, are examined through the Proboscis Extension Response (PER) in honey bees (Apis mellifera). PER is a form of classical conditioning applied to honey bees through scent and reward association which quantifies learning rates. Results between groups treated with thiamethoxam did not differ significantly from untreated control groups. Potential reasons for these results are discussed. The method and experimental apparatus for testing the PER assay are also discussed.


Assessment Of Olfactory-Based Social Recognition: Designing A Paradigm For Marmosets, Stephanie Womack Mar 2017

Assessment Of Olfactory-Based Social Recognition: Designing A Paradigm For Marmosets, Stephanie Womack

UNO Student Research and Creative Activity Fair

Navigation of the social world depends largely on one’s responsiveness to social stimuli and information from a wide range of senses can be used to discriminate between individuals. Mammals use several sensory modes to communicate and respond to their surroundings, but olfaction is the dominant sense across most species. Chemosignals provide a wealth of information including sex, age, reproductive status, and individual identity. Currently, assessment of olfaction is largely limited to the habituation-dishabituation paradigm or the two-choice discrimination task used primarily in rodents. These paradigms are limited by their reliance on inherent reward of interaction with stimuli without providing additional …


Are Weevils Picky Eaters? Community Structure And Host Specificity Of Neotropical Saproxylic Beetles (Coleoptera: Curculionidae), Jhunior A. Morillo Jan 2017

Are Weevils Picky Eaters? Community Structure And Host Specificity Of Neotropical Saproxylic Beetles (Coleoptera: Curculionidae), Jhunior A. Morillo

Dissertations and Theses

Abstract Primary saproxylic beetles play a major role in forest nutrient cycling and making deadwood accessible to other decomposers. Understanding beetle host preferences and patterns of community assembly is critical for their conservation, and for predicting which species might become invasive. This project aims to investigate the ecological and host specificity, as well as the community composition of curculionids in a mosaic of old-growth (OG) and secondary forest on the Osa Peninsula, Costa Rica. The subfamily Scolytinae was expected to be the most species-rich and abundant. Ambrosia beetles were expected to have more generalist species than other curculionids. Old growth …


Sex Differences In Chronic Stress Effects On Cognition In Rodents, Victoria N. Luine, Juan Gomez, Kevin D. Beck, Rachel E. Bowman Jan 2017

Sex Differences In Chronic Stress Effects On Cognition In Rodents, Victoria N. Luine, Juan Gomez, Kevin D. Beck, Rachel E. Bowman

Psychology Faculty Publications

Chronic stress causes deleterious changes in physiological function in systems ranging from neural cells in culture to laboratory rodents, sub-human primates and humans. It is notable, however, that the vast majority of research in this area has been conducted in males. In this review, we provide information about chronic stress effects on cognition in female rodents and contrast it with responses in male rodents. In general, females show cognitive resilience to chronic stressors which impair male cognitive function using spatial tasks including the radial arm maze, radial arm water maze, Morris water maze, Y-maze and object placement. Moreover, stress often …


Live And Dead Coral Cover On Three Reefs And Public Perceptions Of Degradation Around Almirante Bay, Bocas Del Toro, Phoebe Thompson Oct 2016

Live And Dead Coral Cover On Three Reefs And Public Perceptions Of Degradation Around Almirante Bay, Bocas Del Toro, Phoebe Thompson

Independent Study Project (ISP) Collection

Almirante Bay is a lagoonal body of water off the western Caribbean coast of Panama. The islands within the bay are popular tourist destinations, drawing visitors from around the world to explore beaches and coral reefs. However, the reefs in Almirante Bay are continually exposed to both natural and anthropogenic stressors, such as temperature inversions, boat pollution, sedimentation from dredging, and chemical runoff from coastal banana plantations. Because the economy of the area is heavily dependent on tourism, this creates an interesting dynamic in that the main source of income (tourism) could be detrimental to a main attraction of the …


Benthic Algae And Diatom Communities In Seagrass Meadows Under Three Different Human Impact Regimes In Bocas Del Toro, Panamá, Averyl Cheng Oct 2016

Benthic Algae And Diatom Communities In Seagrass Meadows Under Three Different Human Impact Regimes In Bocas Del Toro, Panamá, Averyl Cheng

Independent Study Project (ISP) Collection

In Bocas del Toro, Panamá, widespread tourism has been the main source of revenue and has become an increasing threat to seagrass meadows and the organisms they support. This study aimed to investigate and describe algae and diatom communities under three different regimes of anthropogenic disturbance: high, medium and low human impact. The biodiversity was analyzed by measuring the algae and diatom assemblages with Shannon-Weiner’s Biodiversity Index, Evenness Index and Sorensen’s coefficient. The data obtained from these three diversity indices were compared to the areas along an anthropogenic disturbance gradient of high, medium and low impact. A total of 12 …


Simulating Mars: Student Projects At Mars Desert Research Station (Mdrs), Ashley Hollis-Bussey, Lycourgos Manolopoulos, Marc Carofano, Hiroki Sugimoto, Cassandra Vella, John Herman Apr 2016

Simulating Mars: Student Projects At Mars Desert Research Station (Mdrs), Ashley Hollis-Bussey, Lycourgos Manolopoulos, Marc Carofano, Hiroki Sugimoto, Cassandra Vella, John Herman

Human Factors and Applied Psychology Student Conference

No abstract provided.


An Analysis Of The Interaction Of Methylphenidate And Nicotine In Adolescent Rats: Effects On Bdnf, Elizabeth D. Freeman Aug 2015

An Analysis Of The Interaction Of Methylphenidate And Nicotine In Adolescent Rats: Effects On Bdnf, Elizabeth D. Freeman

Electronic Theses and Dissertations

This investigation was an analysis of the interaction of adolescent exposure to methylphenidate (MPH; trade name: Ritalin) on nicotine sensitization and conditioned place preference (CPP) in a rodent model and underlying mechanisms of this effect. Animals were treated IP with 1 mg/kg MPH or saline using a ―school day‖ regimen of five days on, two days off, from postnatal day (P) 28-50. During the final two weeks of MPH treatment, animals were either behaviorally sensitized to nicotine (0.5 mg/kg free base) or saline for 10 days, or conditioned to nicotine or saline using the CPP behavioral paradigm. In addition, three …


Pathological Effects Of Repeated Concussive Tbi In Mouse Models: Periventricular Damage And Ventriculomegaly, Richard H. Wolferz Jr. May 2015

Pathological Effects Of Repeated Concussive Tbi In Mouse Models: Periventricular Damage And Ventriculomegaly, Richard H. Wolferz Jr.

Honors Scholar Theses

Repeated concussive traumatic brain injury (rcTBI) is the most prominent form of head injury affecting the brain, with an estimated 1.7 million Americans affected each year (Kuhn 2012). Neurologists have been concerned about the danger of repeated head impacts since the 1920’s, but researchers have only begun to understand the long-term effects of rcTBI (McKee 2009). Although symptoms can be as mild as dizziness, current research suggests that multiple concussions can lead to a progressive degenerative brain disease known as chronic traumatic encephalopathy (CTE) (Luo 2008, McKee 2009, Kane 2013). Research on the brain is just beginning to scratch the …


Behavioral Response Of Pacific Lamprey (Entosphenus Tridentatus) To Predator Odors, Laurie L. Porter Jan 2015

Behavioral Response Of Pacific Lamprey (Entosphenus Tridentatus) To Predator Odors, Laurie L. Porter

All Master's Theses

Pacific lamprey (Entosphenus tridentatus), a species facing serious threats to their existence, experience a number of challenges in reaching their desired spawning grounds during the adult migratory phase, and predators are suspected to be one of these challenges. Understanding if Pacific lamprey respond to predator odorants may provide a management tool for use in conjunction with attractants in guiding lamprey to suitable spawning habitat and deterring them from poor habitat. Previous research has failed to explore Pacific lamprey response to predator odorants, although much research exists on attractant odorants. In our study, we tested Pacific lamprey response to …