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Articles 121 - 150 of 339

Full-Text Articles in Laboratory and Basic Science Research

Morph- And Sex-Specific Differences In Corticosterone Of The Arizona Tiger Salamander (Ambystoma Mavortium Nebulosum), Megan Zerger Mar 2021

Morph- And Sex-Specific Differences In Corticosterone Of The Arizona Tiger Salamander (Ambystoma Mavortium Nebulosum), Megan Zerger

Scholars Week

Life history morph, sex, and body condition are traits that may influence stress within salamander populations because of differences in physiology and environmental conditions. Given widespread declines and the effects chronic stress can have on amphibian health, it is important to understand within-population drivers of stress and how population level variation may influence population viability. Thus, the objective of our study was to assess how corticosterone varies within the Arizona tiger salamander (Ambystoma mavortium nebulosum) population at the Mexican Cut Nature Preserve. We used a non-invasive skin swabbing method to collect baseline and elevated corticosterone from paedomorph (aquatic …

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Functional Influence Of 14-3-3 (Ywha) Proteins In Mammals, Elizabeth Barley, Santanu De Jan 2021

Functional Influence Of 14-3-3 (Ywha) Proteins In Mammals, Elizabeth Barley, Santanu De

Mako: NSU Undergraduate Student Journal

The 14-3-3 (YWHA) proteins are homologous, ubiquitous, and conserved in most organisms ranging from plants to animals and play important roles in regulating key cellular events such as cell signaling, development, apoptosis, etc. These proteins consist of seven isoforms in mammals, termed under Greek alphabetization: beta (β), gamma (γ), epsilon (ε), eta (η), tau/theta (τ), sigma (σ), and zeta (ζ). Each of these isoforms can interact with a plethora of binding partners and has been shown to serve a distinct role in molecular crosstalk, biological processes, and disease susceptibility. Protein 14-3-3 isoforms are scaffolding proteins capable of forming homodimers and …

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Comparing The Condition Of Deformed And Non-Deformed Walleyes In Long Lake, Mn, Clayton Beck Jan 2021

Comparing The Condition Of Deformed And Non-Deformed Walleyes In Long Lake, Mn, Clayton Beck

Journal of Earth and Life Science

Walleye Sander vitreus is a popular game fish in Minnesota. Walleye’s have become such a popular game fish for anglers that it has led agencies to raise Walleye in rearing ponds. Farm raised fish can show deformities due to environmental, genetic, nutritional, infectious, and toxic factors. Due to this phenomenon, the purpose of this study was to determine if the condition of the Walleye differed between those with and without deformities in Long Lake, MN. Angling was the method of capture during the months of September 2020 through July 2021. There was no evidence to suggest condition differed between non-deformed …

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Stress Response Of Largemouth And Smallmouth Bass: Barbless Hooks Versus Barbed Hooks C & R, Wesley Steinmetz, Carson Tembrock Jan 2021

Stress Response Of Largemouth And Smallmouth Bass: Barbless Hooks Versus Barbed Hooks C & R, Wesley Steinmetz, Carson Tembrock

Journal of Earth and Life Science

Largemouth Bass Micropterus salmoides and Smallmouth Bass Micropterus dolomieu are two of the most popular species of fish for anglers to target in Minnesota. In this study, Largemouth Bass and Smallmouth Bass stress was characterized by handling time and recovery time. This study compares handling time and recovery time between barbed and barbless hooks. For Largemouth Bass, there was a significant relationship between recovery time and time out of the water for barbed (P = 0.02, R2 = 0.86) and barbless hooks (P < 0.01, R2 = 0.74). For Smallmouth Bass, there was no significant relationship between recovery time and time …

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Effects Of Salt Runoff On Aquatic Macroinvertebrate Colonies, Kendra Fink Jan 2021

Effects Of Salt Runoff On Aquatic Macroinvertebrate Colonies, Kendra Fink

Journal of Earth and Life Science

Aquatic macroinvertebrates bridge the gap in part between primary and secondary consumers, making them ecologically important for most aquatic systems. They are used as biotic indicators for water quality due to their sensitivity to pollutants and may be affected, specifically, by chloride ion concentrations in the water. Water quality can be evaluated by looking at species compositions and their respective tolerance values. To determine long-term effects of road salt on macroinvertebrate colonies, a total of 338 identified macroinvertebrates of 10 families (two generalized to class) at 9 sites were collected from before, after, and between three bridges on Turtle River …

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Estrogen Disruption Of Hypothalamic Neural Activity, Princess Dickson Jan 2021

Estrogen Disruption Of Hypothalamic Neural Activity, Princess Dickson

Cal Poly Humboldt theses and projects

The brain is highly dependent on the endocrine system for proper neurodevelopment, as it plays a key role in many biological processes. Bisphenol A is a chemical found in plastics that has the potential to mimic the effects of Estrogen in the body, at least weakly. People interact with plastic that contains BPA regularly, and people are at risk for exposure even before being born. The abundance of BPA, along with other exogenous estrogens, makes examining the relationship between early exposure and changes in brain activity imperative. The current study aims to establish a relationship between disrupted estrogen function and …

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Factors Influencing Aquatic Invertebrate Diversity In Mine Pit Lakes Along The Mesabi Iron Range, Jeremy Laurich Jan 2021

Factors Influencing Aquatic Invertebrate Diversity In Mine Pit Lakes Along The Mesabi Iron Range, Jeremy Laurich

Journal of Earth and Life Science

Restoration of mine pit lakes for recreation is going to be very important for the future of Minnesota’s Mesabi Iron Range area. As many as 250 lakes will need to be restored in the future along this 100-kilometer-long swath of mining country. Information regarding the organisms that currently inhabit these lakes is important for future restorations to be a success. The focus of this study was to identify the types of aquatic invertebrates present in pit lakes and to identify some of the factors that influence their abundance and diversity. During this study, invertebrate samples were taken along the shore …

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Is There A Relationship Between Aquatic Vegetation And Amphipod Stocking Mortality?, Alaina Taylor Jan 2021

Is There A Relationship Between Aquatic Vegetation And Amphipod Stocking Mortality?, Alaina Taylor

Journal of Earth and Life Science

Amphipod occurrence and abundance has declined in the Prairie Pothole Region (PPR). Wildlife managers have stocked prairie pothole wetlands with Gammarus lacustris since 2018 to establish self-sustaining populations. G. lacustris have been detected in only 2 of 22 stocked basins, suggesting that they have died or failed to reproduce in most stocking attempts. Aquatic plant surveys were conducted in stocked wetlands and compared to data from surveys of wetlands with naturally occurring populations to understand if G. lacustris would be expected to survive in stocked wetlands based on characteristics of the aquatic vegetation communities. SAV coverage was significantly higher in …

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Predicting Total Length Of Fish In Minnesota Lakes Using Bony Structure Dimensions, Jay Holland Jan 2021

Predicting Total Length Of Fish In Minnesota Lakes Using Bony Structure Dimensions, Jay Holland

Journal of Earth and Life Science

This study seeks to establish a relationship between a fish’s total length to the size of various bony structures of prey fishes. Three important prey fish species in Minnesota were selected for examination in this study, including Black Crappie Pomoxis nigromaculatus, Bluegill Lepomis macrochirus, and Yellow Perch Perca flavescens. Length and weight dimensions of each fish’s otoliths, cleithra, and vertebrae were associated to total lengths of the fish they came from. In many dietary studies, the need for these relationships is strongly related to the disfigurement or degradation of prey fish in stomach samples. With accurate enough regression equations, researchers …

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Abiotic Factors Effect On Summertime Fish Sampling, Kyle Oxley Jan 2021

Abiotic Factors Effect On Summertime Fish Sampling, Kyle Oxley

Journal of Earth and Life Science

Fish surveys are vital in understanding various information about a fishery. Surveys lay the groundwork to how an agency views the status of the fishery, are used to set appropriate regulations, and manage stocking. Understanding which areas of a fishery holds the greatest density of fish is key to achieving the most accurate sample that reflects the fishery. The objective of this study was to track fish densities throughout the summer of 2021 and establish if there is a relationship between high fish density locations and specific water column characteristics. Fish density, temperature, and dissolved oxygen were measured bimonthly at …

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Burbot Post-Spawn Diet, Madison Gibson Jan 2021

Burbot Post-Spawn Diet, Madison Gibson

Journal of Earth and Life Science

Burbots have become an increasingly popular game fish among anglers and have become a strong predator within the systems, consuming a wide variety of organisms. Therefore, the objective of this study was to compare male and female burbots diets post-spawn in a system. Burbots angled from Bad Medicine Lake were sacrificed and the stomach contents were observed, categorized by the lowest taxonomic key, and then weighed in grams. Percent by weight was calculated for each category of organisms and presented in a pie charts. In all three analyses, crustaceans were the main source of the diet structure at more than …

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Exploring The Genetic Variability Of Zebra Mussels Dreisenna Polymoprha, Maxwell Carr Jan 2021

Exploring The Genetic Variability Of Zebra Mussels Dreisenna Polymoprha, Maxwell Carr

Journal of Earth and Life Science

Zebra Mussels Dreisenna polymorpha are one of the most well-known invasive species here in the United States. This species can take over watersheds very quickly, causing damage to both the ecosystem and man-made structures. The basic physical behavior of this species has been well documented, but the invasive North American Zebra Mussel is much more aggressive and therefore causes more damage in the Great Lakes region compared to its native waters. Genetic variation may be playing a very large role in how and why D. polymorpha is causing such large problems for stakeholders in the United States. This review cites …

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Effectiveness Of Endothall Treatment In Reducing Potamogeton Crispus In Lake Julia, Jake Sorensen Jan 2021

Effectiveness Of Endothall Treatment In Reducing Potamogeton Crispus In Lake Julia, Jake Sorensen

Journal of Earth and Life Science

Curly leaf pondweed Potamogeton crispus, is a submerged aquatic plant non-native to North America. It was most likely introduced when common carp Cyprinus carpio were intentionally brought to the Midwest. Endothall is an aquatic herbicide commonly used to control curlyleaf pondweed in lakes. This study focused on collecting data from Lake Julia in Sherburne County, MN. Lake Julia is treated annually in the spring to control curlyleaf pondweed. The results show treatment areas saw a significant increase in vegetation density compared to control areas (P < 0.01). Changes in percent vegetation coverage by date were insignificant (P = 0.24). No significant signs of fish kills were observed. Because this study looked at overall vegetation coverage instead of specifically curlyleaf pondweed, we cannot conclude the increase in vegetation coverage was from curlyleaf pondweed alone. Based on the results from Poovey et al. (2002) we would expect lower vegetation coverage in treatment areas instead of the increased vegetation coverage observed in our study. This suggests the possibility that the endothall treatment successfully killed curlyleaf pondweed in treated areas, which allowed for new growth of submerged aquatic plants to take place as water temperatures increased.

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There’S More Than One Way To Beat The Heat: What Anal Probes Reveal About Thermal Responses In Neotropical Beetles (Coleoptera: Cerambycidae)., Emilie I. Dion Jan 2021

There’S More Than One Way To Beat The Heat: What Anal Probes Reveal About Thermal Responses In Neotropical Beetles (Coleoptera: Cerambycidae)., Emilie I. Dion

Dissertations and Theses

Increasing global temperatures have been linked to shifts in the distributions and preferred microhabitats of terrestrial species. This trend is predicted to impact tropical forest insects, which may already be exposed to conditions that bring them close to their thermal maxima. Tropical microhabitats are linked to vertical stratification, because the canopy is hotter, winder, and less humid than the ground. In ectothermic organisms, such as beetles, thermoregulation is dependent on morphological and behavioral traits. This study investigates woodboring cerambycid beetles, to determine how behavioral thermoregulation through microhabitat selection (vertical stratification) is correlated to morphological attributes (color lightness, size, and pubescence) …

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Exposing Lettuce Plants To Cyanobacteria In A Closed Hydroponics System To Reduce Cyanobacterial Growth And Production, Emily Eberly Dec 2020

Exposing Lettuce Plants To Cyanobacteria In A Closed Hydroponics System To Reduce Cyanobacterial Growth And Production, Emily Eberly

Honors Projects

Sandusky Bay is largely populated by cyanobacterial algal blooms, mainly formed by Planktothrix. Fertilizers containing nitrogen and phosphorus run from agricultural lands into the bay, building up excess nutrients forming eutrophic waters. The Planktothrix feed off these nutrients and grow into algal blooms. To determine a potential solution to the growth of these blooms, I implemented a hydroponics system involving Lactuca Sativa for analysis of Planktothrix growth and productivity. Four different nutrient conditions were added to a Planktothrix-only solution and a solution growing Planktothrix with the lettuce in the hydroponics system. The four conditions consisted of no nutrient …

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The Best Predictions In Experimental Biology Are Critical And Persuasive, Douglas S. Fudge, Andy J. Turko Oct 2020

The Best Predictions In Experimental Biology Are Critical And Persuasive, Douglas S. Fudge, Andy J. Turko

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

A powerful way to evaluate scientific explanations (hypotheses) is to test the predictions that they make. In this way, predictions serve as an important bridge between abstract hypotheses and concrete experiments. Experimental biologists, however, generally receive little guidance on how to generate quality predictions. Here, we identify two important components of good predictions – criticality and persuasiveness – which relate to the ability of a prediction (and the experiment it implies) to disprove a hypothesis or to convince a skeptic that the hypothesis has merit. Using a detailed example, we demonstrate how striving for predictions that are both critical and …

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Plankton Dynamics In An Urban, Subtropical Lake Prior To Lake Restoration, Jennifer C. Pulsifer Jul 2020

Plankton Dynamics In An Urban, Subtropical Lake Prior To Lake Restoration, Jennifer C. Pulsifer

LSU Master's Theses

This research assessed plankton dynamics in two urban, subtropical lakes (University Lake and City Park Lake) shortly before a proposed and approved lake restoration project. Plankton dynamics were determined in University Lake by the dilution method to attempt to quantify growth rates of phytoplankton and grazing rates of zooplankton. Landry and Hassett’s (1982) dilution method has been widely used in marine systems to estimate rates of growth and grazing. In many marine systems, nutrients must be added to prevent nutrient limitation. However, it is assumed, due to the hypereutrophic conditions, that nutrients would not be a limitation in University Lake. …

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Protein Phosphatase 2a Suppresses Spindle Elongation In Saccharomyces Cerevisiae, Shoily P. Khondker Jun 2020

Protein Phosphatase 2a Suppresses Spindle Elongation In Saccharomyces Cerevisiae, Shoily P. Khondker

Dissertations, Theses, and Capstone Projects

Eukaryotic cell division is an essential process that is carried out by the cell cycle, a tightly controlled process that has been extensively studied in the budding yeast Saccharomyces cerevisiae. The cell cycle is driven by Cyclin Dependent Kinase (Cdk1) activity. Protein phosphatase 2A-Cdc55 (PP2ACdc55) reverses Cdk1 phosphorylation events during late stages of the cell cycle to ensure the correct order of events. This thesis presents evidence that the anaphase inhibitor Pds1 is a PP2ACdc55 target. Pds1 binds to and inhibits separase (Esp1). Esp1 triggers sister chromatid segregation by cleaving the cohesin complex that holds the …

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Presence Of Dirofilaria Immitis In Mosquitoes In Southeastern Georgia, Angelica C. Tumminello May 2020

Presence Of Dirofilaria Immitis In Mosquitoes In Southeastern Georgia, Angelica C. Tumminello

Honors College Theses

Canine heartworm disease is caused by the filarial nematode Dirofilaria immitis, which is transmitted by at least 25 known species of mosquito vectors. This study sought to understand which species of mosquitoes are present in Bulloch County, Georgia, and which species are transmitting canine heartworm disease. This study also investigated whether particular canine demographics correlated with a greater risk of heartworm disease. Surveillance of mosquitoes was conducted in known heartworm-positive canine locations using traditional gravid trapping and vacuum sampling. Mosquito samples were frozen until deemed inactive, then identified by species and sex. The mosquitoes were then either dissected under …

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Microglia-Neuron Interactions In A Mouse Model Of Low Grade Neuroepithelial Tumors, Veolette Hanna May 2020

Microglia-Neuron Interactions In A Mouse Model Of Low Grade Neuroepithelial Tumors, Veolette Hanna

Honors Scholar Theses

Microglia are the macrophages of the brain and spinal cord, playing an important role in the immune response to disease states of the nervous system. This study conducts an investigation on the activity of microglia in response to low grade neuroepithelial tumors. Using mouse models and microglial markers, a qualitative and quantitative analysis of microglia activation, migration, and invasion within the brain cortex during early stages of tumor development was conducted. It was found that the presence of a low grade neuroepithelial tumor in the cortex of one hemisphere of the brain causes significant microglia activation in comparison to the …

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Understanding Intercellular Signaling During Lung Injury-Repair, Margo Patricia Cain May 2020

Understanding Intercellular Signaling During Lung Injury-Repair, Margo Patricia Cain

Dissertations and Theses (Open Access)

The mammalian lung is a complex system of specialized cell types with precise spatial organization designed to cooperate to perform gas exchange. These cell types together coordinate organ development, homeostasis, and repair after injury through signals either presented or secreted, known as ligands, to be received by receptors on the surface of another, or in some cases, the same cell. The alveolar region of the lung, the primary region of gas exchange, responds to various types of injuries with different lung repair mechanisms. In order to explore how the various cell types in the lung communicate to drive tissue repair …

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158— Optimization And Development Of The Luria-Delbrück Experiment For Geneseo Genetics Labs, Abigail Scheffler Apr 2020

158— Optimization And Development Of The Luria-Delbrück Experiment For Geneseo Genetics Labs, Abigail Scheffler

GREAT Day Posters

The classic genetics experiment by Salvador Luria and Max Delbruck showed that mutations are present prior to selection rather than arising in response to selective pressures. In this experiment, we used Escherichia coli as our model organism and the antibiotics streptomycin and rifampicin to show students in a laboratory setting how this hypothesis can be applied. Specifically, we optimized antibiotic concentrations and the amount of bacterial cells to use in order for the activity to run smoothly and consistently in a student laboratory. We found that the ideal conditions are 1000 cells/ml and 25 micrograms/ml of streptomycin. These concentrations showed …

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Polyphenol-Rich Purple Corn Pericarp Extract Adversely Impacts Herbivore Growth And Development, Mandeep Tayal, Pavel Somavat, Isabella Rodriguez, Tina Thomas, Bradley O. Christoffersen, Rupesh R. Kariyat Feb 2020

Polyphenol-Rich Purple Corn Pericarp Extract Adversely Impacts Herbivore Growth And Development, Mandeep Tayal, Pavel Somavat, Isabella Rodriguez, Tina Thomas, Bradley O. Christoffersen, Rupesh R. Kariyat

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Plant secondary metabolites such as terpenes, phenolics, glycosides, and alkaloids play various functional roles including pigmentation, foliar and floral volatile synthesis, hormonal regulation, and direct and indirect defenses. Among these, phenolic compounds are commonly found in plants, but vary in the distribution of their specific compounds among plant families. Polyphenols, including anthocyanins and tannins, are widely distributed and have been well documented for their roles- primarily in plant pigmentation and also in plant defenses. However, commercialization of such compounds for use in insect pest management is severely hampered by expensive, inefficient, and time-consuming extraction protocols. Using a recently developed inexpensive …

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Water In Your Neighbourhood: A Model For Implementing A Semester-Long Course-Based Undergraduate Research Project In Introductory Biology, Ana Lucia Fuentes, Maria Entezari Jan 2020

Water In Your Neighbourhood: A Model For Implementing A Semester-Long Course-Based Undergraduate Research Project In Introductory Biology, Ana Lucia Fuentes, Maria Entezari

Publications and Research

Undergraduate biology education has changed over the past decade, incorporating an iterative and evidence-based approach. Many educational assessments have confirmed the effectiveness of integrating authentic research and open-ended inquiry into introductory biology courses, demonstrating a significant positive impact on students’ learning and attitude towards STEM majors. Despite these findings, only a handful of Biology instructors in 2-year colleges adopt this approach, and when adopted, most activities constitute a small fraction of these courses. Finding a feasible, sustainable, semester-long, and cost-effective strategy to incorporate authentic research in the curriculum which promotes integrated understanding of science and addresses socio-scientific issues, is a …

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Using Northern Pike And Yellow Perch To Attempt A Trophic Cascade, Bryan Sea Jan 2020

Using Northern Pike And Yellow Perch To Attempt A Trophic Cascade, Bryan Sea

Journal of Earth and Life Science

In the midwestern USA, water clarity has decreased in shallow lakes as a result of large populations of Fathead Minnow Pimephales promelas and agriculture that impact the life that depend on shallow habitats, such as waterfowl, fish, and aquatic plants. Northern Pike Esox lucius and Yellow Perch Perca flavescens were stocked in a few lakes in attempt to create a tropic cascade and reduce populations of nuisance Fathead Minnows. Northern Pike were stocked as fry and Yellow Perch were stocked as adults either pre-spawn or post-spawn. Lake managers hypothesized adult Yellow Perch would spawn and the age-0 Perch would feed …

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Walleye Sander Vitreus Visceral Fat Accumulation, Cole Daleiden, Seth Renner Jan 2020

Walleye Sander Vitreus Visceral Fat Accumulation, Cole Daleiden, Seth Renner

Journal of Earth and Life Science

For ectothermic species, proper lipid retention is critical to surviving in regions with limited growing seasons. Visceral fat is the dominant form of this energy storage in fish. Build up and depletion of these lipids occur seasonally as activity levels change throughout winter and spring. Due to this annual pattern, the purpose of this study is to determine a trend in the amount of visceral fat Walleye Sander vitreus accumulate in a time of year when metabolic rates are low and transition into the spawning process has yet to occur. Angling was the method of capture conducted during the months …

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Diffusion And Osmosis: Passive Movement Of Molecules In Biological Systems, Ana Lucia Fuentes, Maria Entezari Jan 2020

Diffusion And Osmosis: Passive Movement Of Molecules In Biological Systems, Ana Lucia Fuentes, Maria Entezari

Open Educational Resources

During this lab, students determine the salinity of collected water samples. They also compare the effect of the solute concentration of their samples with that of solutions of known solute concentrations on plant cells.

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Non-Invasive Method For Leptin Supplementation In Zebrafish (Danio Rerio), Regan Mcnamara Jan 2020

Non-Invasive Method For Leptin Supplementation In Zebrafish (Danio Rerio), Regan Mcnamara

Williams Honors College, Honors Research Projects

I tested the hypothesis that recombinant leptin protein can be introduced to zebrafish in vivo through non-invasive soaking in a solution containing the protein. One way to study various molecules’ effects in vivo is through intraperitoneal or intracerebroventricular injections during the embryonic or larval stage, which is invasive, difficult to administer, and can have a high mortality rate. 48 hours post fertilization (hpf) zebrafish were soaked in a His-tagged recombinant leptin protein solution at 10 nM and 100 nM concentrations (produced by Genscript). After soaking, zebrafish larvae were washed extensively to remove all recombinant protein on their exterior before homogenization. …

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The Exploration Of Nanotoxicological Copper And Interspecific Saccharomyces Hybrids, Matthew Joseph Winans Phd Jan 2020

The Exploration Of Nanotoxicological Copper And Interspecific Saccharomyces Hybrids, Matthew Joseph Winans Phd

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nanotechnology takes advantage of cellular biology’s natural nanoscale operations by interacting with biomolecules differently than soluble or bulk materials, often altering normal cellular processes such as metabolism or growth. To gain a better understanding of how copper nanoparticles hybridized on cellulose fibers called carboxymethyl cellulose (CMC) affected growth of Saccharomyces cerevisiae, the mechanisms of toxicity were explored. Multiple methodologies covering genetics, proteomics, metallomics, and metabolomics were used during this investigation. The work that lead to this dissertation discovered that these cellulosic copper nanoparticles had a unique toxicity compared to copper. Further investigation suggested a possible ionic or molecular mimicry …

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Inhibition Of Serine, Glycine, One-Carbon Metabolism Reduces Proinflammatory Response, Ashlynn Mccall Dec 2019

Inhibition Of Serine, Glycine, One-Carbon Metabolism Reduces Proinflammatory Response, Ashlynn Mccall

Honor’s College Freeman Research Journal

Acute lung injury affects around 200,000 critically ill patients annually, with a mortality rate near 33%. With a lack of targeted therapies, it is vital to discover and understand the fundamental cellular processes that drive macrophage inflammation. In the field of macrophage immunometabolism, it is accepted that macrophages upregulating glycolysis indicates a role in proinflammatory processes, however, there is a lack of knowledge surrounding the intermediate pathways in glycolysis. This study examined the effects of serine, glycine, one-carbon metabolism inhibition on LPS-induced inflammation in bone marrow-derived macrophages. We show that the enzymatic expression of PHGDH and PSATl is unaffected when …

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