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Laboratory and Basic Science Research Commons™
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Articles 1 - 24 of 24
Full-Text Articles in Laboratory and Basic Science Research
Halloween 2012 Jack O'Lanterns Trick Or Treat, George Mcnamara
Halloween 2012 Jack O'Lanterns Trick Or Treat, George Mcnamara
George McNamara
Halloween 2012 makes trick or treating more visual and interactive than in past years.
the download is a ZIP file containing three files.
Print out the (unnumbered) image on as large and nice printer paper as possible - I used glossy 44" wide here in Miami (University of Miami, MillerSchool of Medicine, Calder Library, Biomedical Communications dept - I also made another print on "fabric", also 44" wide to take with me to an HHMI Janelia Farm conference on 'turning images into knowledge' that ends on Oct 31 - might stay up for a second conference, "GFP..." that start Nov …
Mcnamara 20120831fri-20120904tue Cosmic Ray Particles By Ccd Imaging, George Mcnamara
Mcnamara 20120831fri-20120904tue Cosmic Ray Particles By Ccd Imaging, George Mcnamara
George McNamara
McNamara 20120831Fri-20120904Tue Cosmic Ray Particles by CCD imaging.zip contains image files in support of a Microscopy Today article - please see
http://www.microscopy-today.com/
Cosmic Ray Particles Images With Orca-Ii Erg, George Mcnamara
Cosmic Ray Particles Images With Orca-Ii Erg, George Mcnamara
George McNamara
Cosmic ray particles image series acquired using a Hamamatsu ORCA-II ERG scientific grade CCD camera, cooled to -60 C. Each image is a consecutive 600 second (10 minute) exposure time with no light to the camera.
While processing the data, I discoverd that the background changed around planes 25 and 227 (see Excel file and jpeg screenshots), so I also processed only planes 025-227 (203 planes total, 2030 minutes, 33.83 hours). the CCD industry "rule of thumb" for a "typical" CCD sensor (i.e. 1/3" CCD) is that one cosmic ray particle strikes a sensor approximately every 30 seconds (assuming not …
Analyzing Environmental Microbes For Genomic Regions Promoting Ionic Liquid Tolerance In E. Coli, Ann Nguyen, Alison Richins, Thomas Rüegg, Steven Singer, Michael Thelen
Analyzing Environmental Microbes For Genomic Regions Promoting Ionic Liquid Tolerance In E. Coli, Ann Nguyen, Alison Richins, Thomas Rüegg, Steven Singer, Michael Thelen
STAR Program Research Presentations
Ionic liquids (ILs) are promising as solvents to increase the efficiency of biofuel production; however, ILs are toxic to microbes used in the fermentation of liquid fuels. To engineer IL resistant biofuel hosts, environmental bacteria were screened for tolerance, and these were used to create gene libraries to test in E. coli. Future characterization of these libraries using molecular techniques will be used to identify genes that contribute IL-tolerance to transformed microbes.
Studying Human Disease Genes In Caenorhabditis Elegans : A Molecular Genetics Laboratory Project, Elisabeth A. Cox-Paulson, Theresa M. Grana, Michelle A. Harris, Janet M. Batzli
Studying Human Disease Genes In Caenorhabditis Elegans : A Molecular Genetics Laboratory Project, Elisabeth A. Cox-Paulson, Theresa M. Grana, Michelle A. Harris, Janet M. Batzli
Biological Sciences Research
Scientists routinely integrate information from various channels to explore topics under study. We designed a 4-wk undergraduate laboratory module that used a multifaceted approach to study a question in molecular genetics. Specifcally, students investigated whether Caenorhabditis elegans can be a useful model system for studying genes associated with human disease. In a large-enrollment, sophomore-level laboratory course, groups of three to four students were assigned a gene associated with either breast cancer (brc-1), Wilson disease (cua-1), ovarian dysgenesis (fshr-1), or colon cancer (mlh-1). Students compared observable phenotypes of wild-type C. elegans and C. elegans with a homozygous deletion in the assigned …
But What Is It That You Actually Do? (What It's Really Like Working In The Lab), Michael Reagan
But What Is It That You Actually Do? (What It's Really Like Working In The Lab), Michael Reagan
Headwaters
No abstract provided.
Increased Geranylgeranylated K-Ras Contributes To Antineoplastic Effects Of Farnesyltransferase Inhibitors., Mandy A. Hall
Increased Geranylgeranylated K-Ras Contributes To Antineoplastic Effects Of Farnesyltransferase Inhibitors., Mandy A. Hall
Dissertations & Theses (Open Access)
The Ras family of small GTPases (N-, H-, and K-Ras) is a group of important signaling mediators. Ras is frequently activated in some cancers, while others maintain low level activity to achieve optimal cell growth. In cells with endogenously low levels of active Ras, increasing Ras signaling through the ERK and p38 MAPK pathways can cause growth arrest or cell death. Ras requires prenylation – the addition of a 15-carbon (farnesyl) or 20-carbon (geranylgeranyl) group – to keep the protein anchored into membranes for effective signaling. N- and K-Ras can be alternatively geranylgeranylated (GG’d) if farnesylation is inhibited but are …
Flatbed Scanner Report - Optical Density Dynamic Range, George Mcnamara
Flatbed Scanner Report - Optical Density Dynamic Range, George Mcnamara
George McNamara
George McNamara (now at University of Miami) report for Hua Yu and Richard Jove, City of Hope National Medical Center, on optical density dynamic range of several flatbed scanners.
Introduction To Nanoscopy Nano-Talk, George Mcnamara
Introduction To Nanoscopy Nano-Talk, George Mcnamara
George McNamara
T7-1 is the designation for the LMRG Nanoscopy session at ABRF in Orlando, FL, on March 20, 2012. The PDF file here is a draft of my presentation.
May not be very helpful since (1) would probably help to know what is in my head and each slide will [hopefully] prompt me to say, and (2) 10 minute talk so I am going to push the "next slide" button after saying very little.
__________________
Publisher statement:
The T7-1 Introduction to Nanoscopy Nano Talk is copyrighted (c) George McNamara, 2012. Except for (1) screenshots from research articles (which are copyrighted by …
Pubspectra - Open Data Access Fluorescence Spectra, George Mcnamara
Pubspectra - Open Data Access Fluorescence Spectra, George Mcnamara
George McNamara
The Internet is enabling greater access to spectral imaging publications, spectral graphs, and data than that was available a generation ago. The spectral imaging systems discussed in this issue of Cytometry work because reagent and hardware spectra are reproducible, reusable, and provide input to spectral unmixing and spectral components recognition algorithms. These spectra need to be readily available in order to determine what to purchase, how to use it, and what the output means. We refer to several commercially sponsored and academic spectral web sites and discuss our spectral graphing and data sites. Sites include fluorescent dye graph servers from …
Mcnamara 2011 Feature Extraction (Image Analysis), George Mcnamara
Mcnamara 2011 Feature Extraction (Image Analysis), George Mcnamara
George McNamara
Feature Extraction presentation and movies in a ZIP file from a presentation I gave at ISAC 2011 in Baltomore, Md.
Feature extraction is one phrase for image analysis.
Rogers Pmn Movie - Background Information, George Mcnamara
Rogers Pmn Movie - Background Information, George Mcnamara
George McNamara
Please see
http://mdc.custhelp.com/app/answers/detail/a_id/18689/~/metamatters-newsletters
for my series of MetaMorph MetaMatters articles in volume 2, numbers 3 through 6, on the Rogers PMN Panorama data set.
http://mdc.custhelp.com/app/answers/detail/a_id/18689/~/metamatters-newsletters
Brief summary (for more, see the Word doc)
It’S My Body: The Biomedical Ethics Of Cell And Organ Harvest, Christina Perri
It’S My Body: The Biomedical Ethics Of Cell And Organ Harvest, Christina Perri
Common Reading Essay Contest Winners
First Place
Why Do We Collect?, Allison Marsh
Why Do We Collect?, Allison Marsh
Section 2: Imaging the Microscopic
No abstract provided.
Imaging And Aesthetics, Allison Marsh
Imaging And Aesthetics, Allison Marsh
Section 2: Imaging the Microscopic
No abstract provided.
A Crossword Puzzle For Kids, Allison Marsh
An Exhibit Wordle, Allison Marsh
Word Find, Allison Marsh
Lesson Plans, Activities, And Further Reading, Allison Marsh
Lesson Plans, Activities, And Further Reading, Allison Marsh
Handouts
No abstract provided.
Images From The Exhibit, Page 2, Allison Marsh
Images From The Exhibit, Page 1, Allison Marsh
Spotlight On Usc: A.C. Moore Herbarium, Allison Marsh
Spotlight On Usc: A.C. Moore Herbarium, Allison Marsh
Section 2: Imaging the Microscopic
No abstract provided.
Biology Department Newsletter, No.2, Sacred Heart University
Biology Department Newsletter, No.2, Sacred Heart University
Biology Newsletter
No abstract provided.
Environmental Issues For The 21st Century And Their Impact On Human Health, Richard B. Philp
Environmental Issues For The 21st Century And Their Impact On Human Health, Richard B. Philp
Richard B. Philp
This book (see contents attached) is now available from the author on CD