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Kinesiology Commons

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Full-Text Articles in Kinesiology

Acute Exercise And Oxidative Stress: Crossfit™ Vs. Treadmill Bout, Brian Kliszczewicz, Quindry C. John, Blessing L. Daniel, Oliver D. Gretchen Oct 2015

Acute Exercise And Oxidative Stress: Crossfit™ Vs. Treadmill Bout, Brian Kliszczewicz, Quindry C. John, Blessing L. Daniel, Oliver D. Gretchen

Faculty and Research Publications

CrossFit™, a popular high-intensity training modality, has been the subject of scrutiny, with concerns of elevated risk of injury and health. Despite these concerns empirical evidence regarding physiologic stresses including acute oxidative stress is lacking. Therefore, the purpose of this investigation was to examine the acute redox response to a CrossFit™ bout. Furthermore, these findings were compared to a high-intensity treadmill bout as a point of reference. Ten males 26.4 ± 2.7 yrs having three or more months of CrossFit™ experience participated in the present study. Blood plasma was collected at four time points: Pre-exercise (PRE), immediately-post-exercise (IPE), 1 hr-post …


Supra-Physiological Doses Of Testosterone Affect Membrane Oxidation Of Human Neutrophils Monitored By The Fluorescent Probe C11-Bodipy581/591, Tacito Pessoa De Souza Jr, Andre K. Yamada, Roberto Simao, Tatiana G. Polotow, Rui Curi, Zachary Pope, Jeffrey Willardson, Marcelo P. Barros May 2013

Supra-Physiological Doses Of Testosterone Affect Membrane Oxidation Of Human Neutrophils Monitored By The Fluorescent Probe C11-Bodipy581/591, Tacito Pessoa De Souza Jr, Andre K. Yamada, Roberto Simao, Tatiana G. Polotow, Rui Curi, Zachary Pope, Jeffrey Willardson, Marcelo P. Barros

Faculty Research and Creative Activity

The purpose of this study was to determine the effects of supra-physiological doses of testosterone (TES) on membrane oxidation of activated human neutrophils in vitro using an innovative and sensitive technique: the real-time detection with the fluorescence probe C11-BODIPY581/591. Methodological controls were performed with the lipid-soluble and powerful antioxidant astaxanthin at different neutrophil density cultures. Neutrophils from nine healthy young men (23.4 ± 2.5 years, 174.4 ± 7.0 cm height, and 78.3 ± 7.0 kg weight) were isolated and treated with 0.1 or 10 μM TES for 24 h and subsequently labeled with the free radical-sensitive probe C11-BODIPY581/591 for monitoring …


Relationship Between Circulating Progenitor Cells, Vascular Function And Oxidative Stress With Long-Term Training And Short-Term Detraining In Older Men, Sarah Witkowski, Michael M. Lockard, Nathan T. Jenkins, Thomas O. Obisesan, Espen E. Spangenburg, James M. Hagberg Jan 2010

Relationship Between Circulating Progenitor Cells, Vascular Function And Oxidative Stress With Long-Term Training And Short-Term Detraining In Older Men, Sarah Witkowski, Michael M. Lockard, Nathan T. Jenkins, Thomas O. Obisesan, Espen E. Spangenburg, James M. Hagberg

Exercise and Sport Studies: Faculty Publications

Exercise may contribute to the maintenance of vascular function via enhanced liberation and action of bone-marrow-derived progenitor cells. Activity related changes in oxidative stress may also influence the number and function of these cells. In the present study, we sought to determine (i) whether adaptations in reactive hyperaemic FBF (forearm blood flow) response associated with long-term endurance exercise and short-term detraining were related to resting putative progenitor cell number and function, and (ii) whether oxidative stress affected these factors. Participants included men with a history of more than 30 years of moderate-to-high-intensity exercise (HI group) and healthy low-active age- and …