Immunology of Infectious Disease Commons^™

B Cell Heterogeneity In The Teleost Kidney: Evidence For A Maturation Gradient From Anterior To Posterior Kidney, Patty Zwollo, Suzanne Cole, Erin Bromage, Stephen Kaattari

VIMS Articles

The fish immune system is quite different from the mammalian system because the anterior kidney forms the main site for hematopoiesis in this species. Using transcription factor-specific Abs derived from the murine system, together with anti-trout Ig Abs and Percoll gradient separation, we analyzed B cells from trout kidney sections and compared them to those from spleen and blood. For this study, immune cells were separated by Percoll gradients, and the resulting subpopulations were defined based on expression of B cell-specific transcription factors Pax-5 and B lymphocyte-induced maturation protein-1, as well as proliferative and Ig-secreting properties. Comparison of kidney, blood, …