Immunology and Infectious Disease Commons^™

The Grazing Of Sheep In The Pastoral Areas Of Western Australia : Best Management Practice Guidelines, Department Of Agriculture And Food, Western Australia

Land resources best practice series

The Land Administration Act 1997 requires a pastoralist to manage and work the land within a pastoral lease to its best advantage and use methods of best pastoral and environmental management practices to achieve sustainable land use. The Pastoral Lands Board will use these best practices as a guideline.

Systematics And Emerging Infectious Diseases: From Management To Solution, Daniel R. Brooks, Eric P. Hoberg

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The crisis of emerging infectious disease stems from the absence of comprehensive taxonomic inventories of the world's parasites, which includes the world's pathogens. Recent technological developments raise hopes that the global inventory of species, including potential pathogens, can be accomplished in a timely and cost-effective manner. The phylogenetics revolution initiated by systematists provides a means by which information about pathogen transmission dynamics can be placed in a predictive framework. Increasingly, that information is widely available in digital form on the internet. Systematic biology is well positioned to play a crucial role in efforts to be proactive in the arena of …

Bionomics Of Larvae Of Parelaphostrongylus Odocoilei (Nematoda: Protostrongylidae) In Experimentally Infected Gastropod Intermediate Hosts, Emily J. Jenkins, S. J. Kutz, Eric P. Hoberg, L. Polley

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Parelaphostrongylus odocoilei is a protostrongylid parasite that has recently been recognized at several locations in sub-Arctic, but not Arctic, North America. We investigated factors that may determine the distribution of P. odocoilei, including suitable gastropod intermediate hosts, temperature requirements for larval development in gastropods, and larval emergence facilitating overwinter transmission. We collected and experimentally infected gastropods from a site in the sub- Arctic where P. odocoilei is at the northern limit of its distribution. Deroceras laeve, Catinella sp., and Euconulus cf. fulvus, but not members of the Pupillidae, were suitable intermediate hosts. We describe bionomics of larvae …

Fms-Like Tyrosine Kinase 3 Ligand Recruits Plasmacytoid Dendritic Cells To The Brain, James Curtin, Gwendalyn King, Carlos Barcia, Chunyan Liu, Francois Hubert, Carole Guillonneau, Regis Josien, Ignacio Anegon, Pedro Lowenstein, Maria Castro

Articles

The lack of professional afferent APCs in naive brain parenchyma contributes to the systemic immune ignorance to Ags localized exclusively within the brain. Dendritic cells (DCs) appear within the brain as a consequence of inflammation, but no molecular mechanisms accounting for this influx have been described. In this study we demonstrate that Fms-like tyrosine kinase 3 ligand (Flt3L) recruits plasmacytoid DCs (pDCs; >50-fold; p < 0.001) to the brain parenchyma. These pDCs expressed IFN-alpha, the hallmark cytokine produced by pDCs, indicating recruitment and activation in situ of bona fide pDCs within the brain parenchyma. Flt3L did not increase the numbers of conventional DCs, macrophages, or B, T, NK, NKT, or microglial cells within the brain. Our data demonstrate that Flt3L reconstitutes a crucial afferent component of the immune response, namely, professional APCs within the brain parenchyma, and this could counteract the intrinsic systemic immune ignorance to Ags localized exclusively within the brain.

Transcriptome Signature Of Virulent And Attenuated Pseudorabies Virus-Infected Rodent Brain, Christina Paulus, Patricia J. Sollars, Gary E. Pickard, Lynn W. Enquist

School of Veterinary and Biomedical Sciences: Faculty Publications

Mammalian alphaherpesviruses normally establish latent infections in ganglia of the peripheral nervous system in their natural hosts. Occasionally, however, these viruses spread to the central nervous system (CNS), where they cause damaging, often fatal, infections. Attenuated alphaherpesvirus derivatives have been used extensively as neuronal circuit tracers in a variety of animal models. Their circuit-specific spread provides a unique paradigm to study the local and global CNS response to infection. Thus, we systematically analyzed the host gene expression profile after acute pseudorabies virus (PRV) infection of the CNS using Affymetrix GeneChip technology. Rats were injected intraocularly with one of three selected …

Selachohemecus Benzi N. Sp. (Digenea: Sanguinicolidae) From The Blacktip Shark Carcharhinus Limbatus (Carcharhinidae) In The Northern Gulf Of Mexico, Stephen A. Bullard, Robin M. Overstreet, John K. Carlson

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Selachohemecus benzi Bullard & Overstreet n. sp. infects the heart and kidney of the blacktip shark Carcharhinus limbatus in the northern Gulf of Mexico off Florida and Mississippi, USA. Specimens of S. olsoni Short, 1954, the only congener and only other named blood fluke reported from a chondrichthyan in the Gulf of Mexico, were collected from the heart of the Atlantic sharpnose shark Rhizoprionodon terraenovae from two new localities, Apalachicola Bay, Florida, and Mississippi Sound, Mississippi, USA. The new species differs from S. olsoni by having a larger body (1.4–3.8 mm long), robust tegumental body spines numbering 51–63 along each …

Variation In The Innate And Acquired Arms Of The Immune System Among Five Shorebird Species, Luisa Mendes, Theunis Piersma, Dennis Hasselquist, Kevin Matson, Robert Ricklefs

Biology Department Faculty Works

To contribute to an understanding of the evolutionary processes that shape variation in immune responses, we compared several components of the innate and acquired arms of the immune system in five related, but ecologically diverse, migratory shorebirds (ruff Philomachus pugnax L., ruddy turnstone Arenaria interpres L., bar-tailed godwit Limosa lapponica L., sanderling Calidris alba Pallas and red knotC. canutus L.). We used a hemolysis-hemagglutination assay in free-living shorebirds to assess two of the innate components (natural antibodies and complement-mediated lysis), and a modified quantitative enzyme-linked immunosorbent assay in birds held in captivity to assess the acquired component (humoral antibodies against …

Antibiotic Resistant And Coliform Bacteria In The Ohio River; 2002 To 2004, Lisa Marie Smith

Theses, Dissertations and Capstones

During 2002 and 2003 samples, collected every five miles along the Ohio River, were analyzed for total cultivable bacteria, ciprofloxacin resistant bacteria, erythromycin resistant bacteria, tetracycline resistant bacteria, and fecal coliforms. During 2004 samples were analyzed for total cultivable bacteria, ciprofloxacin resistant bacteria, sulfamethizole resistant bacteria, tetracycline resistant bacteria, Virginiamycin resistant bacteria, total coliforms, and Escherichia coli. The objectives of this study were to systematically collect data on fecal coliforms, E. coli, and antibiotic resistant bacteria in the Ohio River and its major tributaries; to determine if antibiotic resistance populations are correlated to each other or to coliforms; and …

Differential Expression Of Immune Response Genes In Steller Sea Lions (Eumetopias Jubatus): An Indicator Of Ecosystem Health?, Lizabeth Bowen, Brian Aldridge, Kimberlee Beckmen, Tom Gelatt, Lorrie Rea, Kathy Burek, Ken Pitcher, Jeffrey L. Stott

United States Department of Commerce: Staff Publications

Characterization of the polygenic and polymorphic features of the Steller sea lion major histocompatibility complex (MHC) provides an ideal window for evaluating immunologic vigor of the population and identifying emergence of new genotypes that reflect ecosystem pressures. MHC genotyping can be used to measure the potential immunologic vigor of a population. However, since ecosystem-induced changes to MHC genotype can be slow to emerge, measurement of differential expression of these genes can potentially provide real-time evidence of immunologic perturbations. MHC DRB genes were cloned and sequenced using peripheral blood mononuclear leukocytes derived from 10 Steller sea lions from southeast Alaska, Prince …

Glucocorticoid Hormone Levels Increase With Group Size And Parasite Load In Cliff Swallows, Samrrah A. Raouf, Linda C. Smith, Mary Bomberger Brown, John C. Wingfield, Charles R. Brown

School of Biological Sciences: Faculty Publications

Animals often cope with adverse events by releasing glucocorticoid hormones, which in turn promote increased energy assimilation. In captive animals, crowding also leads to increased glucocorticoid activity, probably because of increased levels of social competition. We investigated how group size and ectoparasite infestations affected endogenous levels of the glucocorticoid hormone, corticosterone, in colonial cliff swallows, Petrochelidon pyrrhonota, in southwestern Nebraska, USA. Parasites were removed from some colonies by fumigating nests. Baseline levels of corticosterone in breeding adults varied significantly with whether parasites were present, colony size (measured by total number of active nests at a site), and nesting stage. …

Proceedings Of The Western Australian Dhufish Workshop 2004, Mark Pagano, Terry Fuller

Fisheries Occasional Publications

The Western Australian Dhufish Workshop 2004 was a joint project between Recfishwest and the Western Australian Department of Fisheries.

The workshop incorporated presentations from the Department of Fisheries, Recfishwest, the Western Australian Fishing Industry Council, Murdoch University and Challenger TAFE to showcase current knowledge relating to the iconic dhufish in Western Australia. The perspectives of a recreational fisher, a commercial wetline operator and a metropolitan charter boat operator were also presented.

Through information sharing and discussion the workshop provided a platform to better understand the fundamental issues that underpin current and future management arrangements for this valuable resource. Both commercial …

Intercellular Spreading Of Porphyromonas Gingivalis Infection In Primary Gingival Epithelial Cells, Özlem Yilmaz, Philippe Verbeke, Richard J. Lamont, David M. Ojcius

All Dugoni School of Dentistry Faculty Articles

Porphyromonas gingivalis, an important periodontal pathogen, is an effective colonizer of oral tissues. The organism successfully invades, multiplies in, and survives for extended periods in primary gingival epithelial cells (GECs). It is unknown whether P. gingivalis resides in the cytoplasm of infected cells throughout the infection or can spread to adjacent cells over time. We developed a technique based on flow cytofluorometry and fluorescence microscopy to study propagation of the organism at different stages of infection of GECs. Results showed that P. gingivalis spreads cell to cell and that the amount of spreading increases gradually over time. There was a …

Susceptibility Of Primary Eosinophils To Infection With Hiv-1 Strain Htlv-Iiib, Jai Govind Marathe

Browse all Theses and Dissertations

Over the past two decades, much research has been done in the field of human immunodeficiency virus (HIV) and acquired immunodeficiency syndrome (AIDS). However, many of the aspects of pathogenesis of HIV infection and its persistence in the body, despite treatment, remain a mystery. Recent evidence suggests that HIV positive patients develop eosinophilia, especially in the later stages of infection and AIDS. Eosinophils are CD4 positive cells that have the potential to be infected by HIV. Studies have shown that an eosinophilic cell line, AML14.3D10, can be productively infected with a T-cell tropic, CXCR4-using (X4) strain of HIV-1. In this …

Phenotypic Characterization Of Escherichia Coli Strains Taken From Human Intestinal And Urinary Tracts, Kruthi Murthy

Browse all Theses and Dissertations

Escherichia coli (E. coli) is a gram negative bacterium commonly found as a commensal in the intestinal tract of humans and other warm blooded animals. The commensal strains of E. coli are non-pathogenic and do not cause an infection in the host. However, some strains of E. coli are pathogenic and can cause several diseases in humans that include neonatal meningitis, intestinal infections and urinary tract infections. Almost 80-85% of the uncomplicated urinary tract infections are caused by uropathogenic E. coli. Our project involved the characterization of 12 strains of E. coli, isolated from humans in health and disease. These …

Premature Induction Of An Immunosuppressive Regulatory T Cell Response During Acute Simian Immunodeficiency Virus Infection, Jacob D. Estes, Qingsheng Li, Matthew R. Reynolds, Stephen W. Wietgrefe, Lijie Duan, Timothy Schacker, Louis J. Picker, David I. Watkins, Jeffrey D. Lifson, Cavan Reilly, John V. Carlis, Ashley T. Haase

Qingsheng Li Publications

Here we report the results of an investigation into the possibility that one mechanism responsible for the establishment of persistent human immunodeficiency virus infection is an early regulatory T (T_reg) cell response that blunts virus- specific responses. Using the simian immunodeficiency virus (SIV)–infected rhesus macaque model, we show that, indeed, viral replication and immune activation in lymphatic tissue drive a premature immunosuppressive response, with dramatic increases in the frequencies of CD4⁺CD25⁺FOXP3⁺ Treg cells, transforming growth factor–β1⁺ cells, interleukin–10+ cells, and indoleamine 2,3-dioxygenase+CD3⁺ cells.When we compared SIV infection with rhesus cytomegalovirus (RhCMV) …

Parelaphostrongylus Odocoilei In Columbia Black-Tailed Deer From Oregon, Jack A. Mortenson, Arthur Abrams, Benjamin M. Rosenthal, Detiger Dunams, Eric P. Hoberg, Robert J. Bildfell, Richard L. Green

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Documenting the occurrence of Parelaphostrongylus odocoilei has historically relied on the morphological examination of adult worms collected from the skeletal muscle of definitive hosts, including deer. Recent advances in the knowledge of protostrongylid genetic sequences now permit larvae to be identified. Dorsal-spined larvae (DSLs) collected in 2003–2004 from the lung and feces of six Columbian black-tailed deer (Odocoileus hemionus columbianus) from Oregon were characterized genetically. The sequences from unknown DSLs were compared to those from morphologically validated adults and larvae of P. odocoilei at both the second internal transcribed spacer (ITS-2) of ribosomal DNA and the mitochondrial cytochrome …

Tannerella Forsythia, A Periodontal Pathogen Entering The Genomic Era, Anne C. R. Tanner, Jacques Izard

Department of Food Science and Technology: Faculty Publications

Several questions need to be addressed to evaluate whether Tannerella forsythia is to be considered a periodontal pathogen. T. forsythia has been detected in periodontal health and disease, so could it be a pathogen? The species was not detected in many studies despite finding other putative pathogens, so could it be important in pathogenicity? The challenges of working with T. forsythia include its fastidious and anaerobic growth requirements for cultural detection. Thus, studies associating T. forsythia with periodontal and other oral infections have used noncultural approaches (immunoassays and DNA-based assays) in addition to cultural approaches. We feel …

In Memorium: Lynn Ann Hertel, Donald Duszynski, Eric S. Loker

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A memorial tribute to Lynn Ann Hertel (1951-2005) of the University of New Mexico, by Donald W. Duszynski and Eric S. Loker.

Seven New Species Of Eimeria Schneider, 1875 (Apicomplexa: Eimeriidae) From Colubrid Snakes Of Guatemala And A Discussion Of What To Call Ellipsoid Tetrasporocystic, Dizoic Coccidia Of Reptiles, I. M. Asmundsson, Donald W. Duszynski, J. A. Campbell

Harold W. Manter Laboratory of Parasitology: Faculty Publications

During a survey of Guatemalan herpetofauna in the summers of 1998–2000, 29 presumed new species of Eimeria Schneider, 1875 were found, seven of which have a distinct elongate-ellipsoidal shape (L/W ratio ≥ 1.7) and are described herein. Six of the seven new species are similar in oöcyst length, width and L/W ratio and sporocyst length, width and L/W ratio, lack a micropyle, oöcyst residuum, Stieda body, sub- and parastieda bodies, have a polar granule and sporocyst residuum, and their sporocysts appear to have dehiscence sutures. The seventh is slightly smaller and has sporocysts with a Stieda body. The new species …

A New Species Of Neohaematotrephus (Digenea: Echinostomiformes: Cyclocoelidae) In The Spotted-Sandpiper Actitis Macularia (Charadriiformes: Scolopacidae) From Venezuela, David Zamparo, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Fischthal and Nasir (1974) reported Neohaematotrephus brasilianum (as Cyclocoelum brasilianum) in the spotted sandpiper Actitis macularia from Venezuela. Three voucher specimens from that report, deposited in the United States National Parasite Collection, however, differ from N. brasilianum by having the cirrus sac on the sinistral side of the body, which resembles N. facioi, N. arayae, and N. gendrei. The new species is similar to N. brasilianum by having vitelline follicles extending well anterior to the intestinal bifurcation and by having a short and laterally displaced cirrus sac whose posterior end does not reach the intestinal bifurcation, …

First Report Of Mites And Fleas Associated With Sigmodontine Rodents From Corrientes Province, Argentina, Marcela Lareschi, Donald Gettinger, Santiago Nava, Agustín Abba, Mariano L. Merino

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Fleas (Insecta: Siphonaptera) and mesostigmate mites (Acari: Laelapidae) are common ectoparasitic associates of sigmodontine rodents in the Neotropics of South America. In Argentina, most of the research on ectoparasitic arthropods has been carried out on rodents from Buenos Aires Province (Autino and Lareschi, 1998; Lareschi and Mauri, 1998), while only a few small studies have been done in other areas of the country, such as the northwest (Lareschi et al., 2003a), and the Monte Desert Biome in Mendoza Province (Lareschi et al., 2004). Unfortunately, very little information is available from the region between the Paraná and Uruguay rivers in northeastern …

Redescription Of Cryptocotyle Thapari Mcintosh, 1953 (Trematoda: Heterophyidae), In The River Otter Lutra Longicaudis From Bolivia, Scott Lyell Gardner, Peter T. Thew

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Cryptocotyle thapari McIntosh, 1953 (Digenea: Heterophyidae), collected originally from a South American otter that died at the U.S. National Zoo in Washington, D.C., is redescribed from specimens obtained from a river otter, Lutra longicaudis, collected from the Rı´o Matos in the Departamento de Beni, Bolivia. These individuals of C. thapari are slightly smaller than the type specimens, but they have similar features, including testes arranged in tandem and a well-defined ventrogenital sac. We redescribe this species because the original description was based on only 3 individuals, and the figures lacked detail. This is the first record of Cryptocotyle from …

Sl1 Rna Gene Recovery From Enterobius Vermicularis Ancient Dna In Pre-Columbian Human Coprolites, Alena M. Iñiguez, Karl Reinhard, Marcelo Luiz Carvalho Gonçalves, Luiz Fernando Ferreira, Adauto Araújo, Ana Carolina Paulo Vincente

Karl Reinhard Publications

Enterobius vermicularis, pinworm, is one of the most common helminths worldwide, infecting nearly a billion people at all socio-economic levels. In prehistoric populations the paleoparasitological findings show a pinworm homogeneous distribution among hunter-gatherers in North America, intensified with the advent of agriculture. This same increase also occurred in the transition from nomad hunter-gatherers to sedentary farmers in South America, although E. vermicularis infection encompasses only the ancient Andean peoples, with no record among the pre-Colombian populations in the South American lowlands. However, the outline of pinworm paleo epidemiology has been supported by microscopic finding of eggs recovered from coprolites. …

Psettarium Anthicum Sp. N. (Digenea: Sanguinicolidae) From The Heart Of Cobia Rachycentron Canadum (Rachycentridae) In The Northern Gulf Of Mexico, Stephen A. Bullard, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Psettarium anthicum sp. n. (Digenea: Sanguinicolidae) infects the myocardium and atrial wall of the cobia Rachycentron canadum (Linnaeus, 1766) (Rachycentridae) in the northern Gulf of Mexico off Mississippi, USA. It is the first member of Psettarium Goto et Ozaki, 1930 reported from other than the Indian Ocean or Pacific Ocean and the second species of the genus reported from cobia. It differs from its congeners by the combination of having posterior caeca with lateral projections appearing as thorns in lateral view and the male pore anterior to the oötype. The species of Psettarium, P. japonicum (Goto et Ozaki, 1929) …

A Review Of Polylekithum Arnold 1934 And Its Familial Affinities Using Morphological And Molecular Data, With Description Of Polylekithum Catahoulensis Sp. Nov., Stephen S. Curran, Vasyl V. Tkach, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The type material of Polylekithum ictaluri, P. halli, and Maculifer chandleri was examined from the United States National Parasite Museum, and we determined that the material was conspecific, making P. halli and M. chandleri junior subjective synonyms of P. ictaluri. Polylekithum catahoulensis sp. nov. was described from material collected from catfishes at the Catahoula Wildlife Refuge, LaSalle Parish, Louisiana, USA, and compared with P. ictaluri collected from catfishes in Reelfoot Lake, Obion County, Tennessee, USA, and the Pearl River, Hancock County, Mississippi, USA. Polylekithum catahoulensis had smaller eggs (77-–88 μm long by 51–-63 µm wide vs. 94–-108 …

Myxozoan Genera: Definition And Notes On Taxonomy, Life-Cycle Terminology And Pathogenic Species, Jiří Lom, Iva Dyková

Harold W. Manter Laboratory of Parasitology: Library Materials

A list of myxozoan genera is presented in the current taxonomical scheme. These genera are defined; their type species and most important pathogens along with their hosts are listed. Simultaneously, definitions of actinospore stages representing sexual stages of the myxosporean life cycle are given; altogether, 17 actinospore collective groups with 180 types have been described. Life cycles of the two classes of the phylum Myxozoa, Malacosporea and Myxosporea, are briefly outlined with specification of the appropriate terms. Up to now, 4 malacosporean and 2,180 myxosporean species assigned to a total of 62 genera, have been established. The surviving classification of …