Immunology and Infectious Disease Commons^™

The Nematode Fauna Of Long-Nosed Mice Oxymycterus Spp. From The Bolivian Yungas, F. Agustin Jimenez-Ruiz, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty Publications

During a long-term survey of the parasites of mammals from all over Bolivia, 44 individuals of Oxymycterus inca and 6 of Oxymycterus paramensis were collected and examined for parasites from the foothills and Eastern Cordillera (Los Yungas) of the Andes of Bolivia. Three species of nematode were found including, from the cecum, a previously unknown genus and species of pinworm, Caroloxyuris boliviensis n. gen, n. sp., aspidoderids representing Nematomystes rodentophilus, and from the stomach, Protospirura numidica criceticola. These helminths occurred in prevalences of 25, 9, and 14%, respectively, in O. paramensis. Caroloxyuris boliviensis resembles species included in …

Role Of Proapoptotic Bax In Propagation Of Chlamydia Muridarum (The Mouse Pneumonitis Strain Of Chlamydia Trachomatis) And The Host Inflammatory Response, Jean-Luc Perfettini, David M. Ojcius, Charles W. Andrews Jr., Stanley J. Korsmeyer, Roger G. Rank, Toni Darville

All Dugoni School of Dentistry Faculty Articles

The BCL-2 family member BAX plays a critical role in regulating apoptosis. Surprisingly, bax-deficient mice display limited phenotypic abnormalities. Here we investigate the effect of BAX on infection by the sexually transmitted pathogen,Chlamydia muridarum (the mouse pneumonitis strain ofChlamydia trachomatis). Bax −/−cells are relatively resistant to Chlamydia-induced apoptosis, and fewer bacteria are recovered after two infection cycles from Bax −/− cells than from wild-type cells. These results suggest that BAX-dependent apoptosis may be used to initiate a new round of infection, most likely by releasingChlamydia-containing apoptotic bodies from infected cells that could be internalized by neighboring uninfected cells. Nonetheless, infected …

Characterization Of A Gene Encoding Two Isoforms Of A Mitochondrial Protein Upregulated By Cyclosporin A In Activated T Cells, Laurent Mascarell, Rodolphe Auger, Andres Alcover, David M. Ojcius, Thomas Jungas, Veronique Cadet-Daniel, Jean M. Kanellopoulos, Paolo Truffa-Bacchi

All Dugoni School of Dentistry Faculty Articles

Cyclosporin A (CSA) is an immunosuppressor used in organ transplantation. A recent proteomic analysis has revealed that activation of T cells in the presence of CSA induces the synthesis of hundreds of new proteins. Here we used representational difference analysis to characterize some of the corresponding induced genes. After cDNA bank screening we focused on one of these genes, which we named CSA-conditional, T cell activation-dependent (CSTAD) gene. This gene produces two mRNAs resulting from alternative splicing events. They encode two proteins of 104 and 141 amino acids, CSTADp-S and CSTADp-L, for the short and long forms, respectively. FK506 had …

Host Specificity Among Ancyrocephalinae (Monogenoidea) Of Nebraska Sunfish, Megan R. Collins, John J. Janovy Jr.

John J. Janovy, Jr. Publications

This study addressed the problem of local patterns of host specificity among Ancyrocephalinae (Monogenoidea) on bass and sunfish species, when the hosts occur in different species combinations in separate ponds. One hundred fifty-three fish of the Centrarchidae, from four study sites in Nebraska, were collected. Host species included bluegill (Lepomis macrochirus), green sunfish (L. cyanellus), largemouth bass (Micropterus salmoides), black crappie (Pomoxis nigromaculatus), white crappie (P. annularis), and rock bass (Ambloplites rupestris). These fish occurred in different species combinations, depending on the pond sampled. Results indicated that several …

Enterobius Vermicularis: Ancient Dna From North And South American Human Coprolites, Alena M. Iñiguez, Karl J. Reinhard, Adauto Araújo, Luiz F. Ferreira, Ana Carolina P. Vicente

School of Natural Resources: Faculty Publications

A molecular paleoparasitological diagnostic approach was developed for Enterobius vermicularis. Ancient DNA was extracted from 27 coprolites from archaeological sites in Chile and USA. Enzymatic amplification of human mtDNA sequences confirmed the human origin. We designed primers specific to the E. vermicularis 5S ribosomal RNA spacer region and they allowed reproducible polymerase chain reaction identification of ancient material. We suggested that the paleoparasitological microscopic identification could accompany molecular diagnosis, which also opens the possibility of sequence analysis to understand parasite-host evolution.

Bibliography Of Helminth Species Described From African Vertebrates 1800-1967, Albert G. Canaris, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Parasite lists, hosts lists, and bibliographies for Trematoda, Cestoda, Nematoda, and Acanthocephala.
100 pages.

Trends. Security, Stigma, And Biological Weapons, Ibpp Editor

International Bulletin of Political Psychology

This Trends article discusses the political psychology of security concerns pertaining to unclassified information relating to biological weapons and/or biosecurity and how it might be used to harm US security.

Regulation Of Type 1 Diabetes By A Self-Mhc Class Ii Peptide: Role Of Transforming Growth Factor Beta (Tgf-Beta)., Bhagirath Singh, R Mukherjee, P Chaturvedi, Babita Agrawal

Microbiology & Immunology Publications

The present study was undertaken to analyze the regulatory T cells generated in response to class I derived self-I-A beta(g7) (54-76) peptide. It was observed T cells from young unprimed type 1 diabetes (T1D) prone NOD mice did not respond to self-I-A beta(g7) (54-76) peptide although T cells from primed young NOD mice showed a strong response. T cells from young unprimed BALB/c mice responded to self-I-A beta(d) (62-78) peptide. However, a breakdown of tolerance to these peptides was observed with age in both the strains. Culture supernatant from I-A beta(g7) (54-76) peptide-primed cells secreted large amounts of TGF-beta and …

Isovolumetric Regulation In Mammal Cells: Role Of Taurine, B. Ordaz, R. Franco, National University Of Mexico Tuz

School of Veterinary and Biomedical Sciences: Faculty Publications

The ability to regulate cell volume is an ancient conserved trait present in essentially all species through evolution. The maintenance of a constant cell volume is a homeostatic imperative in animal cells. Changes in cell water content affecting the concentration of intracellular messenger molecules impair the complex signaling network, crucial for cell functioning and intercellular communication. Although the renal homeostatic mechanisms exert a precise control of extracellular fluid osmolarity, this is challenged in a variety of pathological situations. The intracellular volume constancy is continuously compromised by the generation of local and transient osmotic microgradients, associated with nutrients uptake, secretion, cytoskeleton …

American Society Of Parasitologists Newsletter, V. 25, No. 4, Winter 2003, Scott Lyell Gardner

American Society of Parasitologists: Newsletter

An issue of the American Society of Parasitologists' quarterly newsletter, also called the Journal of Parasitology Newsletter.

Fleas And Lice Of Mammals In New Mexico, Paulette L. Ford, Richard Fagerlund, Donald W. Duszynski, Paul Polechla

Harold W. Manter Laboratory of Parasitology: Faculty Publications

All available records are compiled for three orders of ectoparasites of mammals in New Mexico: fleas (Siphonaptera), sucking lice (Anoplura), and chewing lice (Mallophaga). We have drawn from records at the University of New Mexico’s Museum of Southwestern Biology, the Vector Control Program of the New Mexico Environment Department, the Environmental Health Department of the City of Albuquerque, and several private collections. We list 99 species of fleas, 27 species of sucking lice, and two species of chewing lice. Included are appendices that list recorded ectoparasite species and their hosts in New Mexico and the counties …

The Blue Crab: Diseases, Parasites And Other Symbionts, Jeffrey D. Shields, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A critical review of the microbial diseases, parasites, and other symbionts of the blue crab, Callinectes sapidus.

Cystic Echinococcosis In The Arctic And Sub-Arctic, Robert L. Rausch

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The northern biotype of Echinococcus granulosus occurs throughout the holarctic zones of tundra and taiga, from eastern Fennoscandia to the Bering Strait in Eurasia and in North America from arctic Alaska approximately to the northern border of the United States. The cycle of the cestode is complex in taiga at lower latitudes, because of the greater diversity of potential hosts. In the Arctic and Subarctic, however, four patterns of predator/prey relationships may be discerned. Two natural cycles involve the wolf and wild reindeer and the wolf and elk (moose), respectively. Where deer of the two species coexist, both are prey …

A Pcr-Elisa For The Identification Of Cyathostomin Fourth-Stage Larvae From Clinical Cases Of Larval Cyathostominosis, J. E. Hodgkinson, J. Ralph Lichtenfels, T. S. Mair, P. Cripps, K. L. Freeman, Y. H. Ramsey, S. Love, J. B. Matthews

Harold W. Manter Laboratory of Parasitology: Faculty Publications

We report the use of six oligoprobes designed from intergenic spacer region sequences to identify fourth-stage larvae (L4) of the tribe Cyathostominae. Oligoprobes were designed for identification of the following species: Cylicocyclus ashworthi, Cylicocyclus nassatus, Cylicocyclus insigne, Cyathostomum catinatum, Cylicostephanus goldi, and Cylicostephanus longibursatus. A seventh probe was designed as a positive control to identify all these members of the Cyathostominae. The intergenic spacer region was amplified by PCR using conserved primers. Initially, three oligoprobes were used in Southern blot analysis. To facilitate high-throughput identification, these and a further four oligoprobes were developed for use in a PCR–ELISA. …

An Arthropod Defensin Expressed By The Hemocytes Of The American Dog Tick, Dermacentor Variabilis (Acari: Ixodidae), Shane M. Ceraul, Daniel E. Sonenshine, Robert E. Ratzlaff, Wayne L. Hynes

Biological Sciences Faculty Publications

Both soluble and cell-mediated components are involved in the innate immune response of arthropods. Injection of Borrelia burgdorferi, the Lyme disease agent, results in the secretion of defensin into the hemolymph of the ixodid tick, Dermacentor variabilis. The presence of the peptide is observed as early as 15 min post-challenge and remains present through 18 h post-challenge. As observed in insects and soft ticks, the transcript for defensin is detected as early as 1 h post-challenge in D. variabilis. RT-PCR resulted in an amplicon of 624 bp with a 225 bp region that translates to a 74 …

Use Of Irradiated And Formalin-Fixed Trichomonas Vaginalis To Examine Protective Immune Responses In The Mouse Intraperitoneal Model, Terryl L. Hostetler, James J. Daly, Timothy J. O'Brien, Max L. Baker

Journal of the Arkansas Academy of Science

Gamma irradiated Trichomonas vaginalis was used to initiate a protective immune response in mice by intraperitoneal inoculation using host mortality as the measure of virulence. Irradiated trichomonads of a virulent strain gave some immune protection but no more so than the use of formalin-fixed, virulent trichomonads. A formalin-fixed virulent strain combined with complete Freund's adjuvant (CFA) gave complete protection. Metabolically produced antigens do not appear to be important in conferring protective immunity in these experiments. A common laboratory strain of T. vaginalis (ATCC 30001) was used as an avirulent control. It gave no protection against a virulent strain. Combining CFA …

Osmosensitive Taurine Release: Does Taurine Share The Same Efflux Pathway With Chloride And Other Amino Acid Osmolytes?, Rodrigo Franco

School of Veterinary and Biomedical Sciences: Faculty Publications

Swelling subsequent to hyposmotic conditions activates a process of volume regulation present in most cell types. This volume adjustment is accomplished by osmolyte translocation toward the extracellular space to reach a new osmotic equilibrium. Molecules involved in this homeostatic mechanism have been broadly classified into two categories: organic and inorganic osmolytes. Inorganic osmolytes comprise mainly the intracellular ions K⁺ and Cl^–. Cell swelling–induced activation of separate K⁺ and Cl^– channels has been described in most preparations. Organic osmolytes are grouped in three categories: amino acids, polyalcohols, and methylamines. These osmolytes, particularly taurine, are present in …

Role Of Activator Protein-1 In The Down-Regulation Of The Human Cyp2j2 Gene In Hypoxia, Nicole Y. Marden, Eva Fiala-Beer, Shi-Hua Xiang, Michael Murray

School of Veterinary and Biomedical Sciences: Faculty Publications

The cytochrome P450 (CYP) 2J2 arachidonic acid epoxygenase gene was down-regulated at a pre-translational level in human hepatoma-derived HepG2 cells incubated in a hypoxic environment; under these conditions, the expression of c-Jun and c-Fos mRNA and protein was increased. The 5′-upstream region of the CYP2J2 gene was isolated by amplification of a 2341 bp fragment and putative regulatory elements that resembled activator protein-1 (AP-1)-like sequences were identified. From transient transfection analysis, c-Jun was found to strongly activate a CYP2J2–luciferase reporter construct, but co-transfection with plasmids encoding c-Fos or c-Fos-related antigens, Fra-1 and -2, abrogated reporter activity. Using a series …

A New Species Of Laelapine Mite (Acari: Parasitiformes: Laelapidae) Associated With Proechimys Dimidiatus In The Atlantic Forests Of Brazil, Donald Gettinger, Helena Godoy Bergallo

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Tur megistoproctus, a new species of Laelapinae, is described from the pelage of the echimyid rodent Proechimys dimidiatus from the Atlantic forests of Ilha Grande, south of Rio de Janeiro. Measurements and illustrations are included for females and males. Another laelapine mite species, Tur turki Fonseca, co-occurred with T.megistoproctus in our studies and was recorded from the same host individuals and localities. These 2 laelapine mite species appear to be exclusively associated with a complex of echimyid rodent species (subgenus Trinomys) in the Atlantic forests of southeastern Brazil.

A New Species Of Neotropical Laelapine Mite (Acari: Parasitiformes, Laelapidae) From The Marsupial Monodelphis Americana, Donald Gettinger, Fernanda Martins-Hatano

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Androlaelaps ilhacardosoi, a new species of Laelapinae, is described from the pelage of a 3-lined marsupial, Monodelphis americana, collected on Ilha Cardoso, São Paulo State, Brazil. The description is based on the female, and the systematics of laelapine mites associated with neotropical marsupials is discussed.

Phylogenetic Analysis Of The Metastrongyloidea (Nematoda: Strongylida) Inferred From Ribosomal Rna Gene Sequences, Ramon A. Carreno, Steven A. Nadler

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Phylogenetic relationships among nematodes of the strongylid superfamily Metastrongyloidea were analyzed using partial sequences from the large-subunit ribosomal RNA (LSU rRNA) and small-subunit ribosomal RNA (SSU rRNA) genes. Regions of nuclear ribosomal DNA (rDNA) were amplified by polymerase chain reaction, directly sequenced, aligned, and phylogenies inferred using maximum parsimony. Phylogenetic hypotheses inferred from the SSU rRNA gene supported the monophyly of representative taxa from each of the 7 currently accepted metastrongyloid families. Metastrongyloid taxa formed the sister group to representative trichostrongyloid sequences based on SSU data. Sequences from either the SSU or LSU RNA regions alone provided poor resolution for …

Coccidia In The Mammary Glands Of Shrews (Order: Insectivora), Donald W. Duszynski, William C. Marquardt

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Three of 6 female long-clawed shrews, Sorex unguiculatus Dobson, 1890, collected on the island of Hokkaido, Japan, were found to have unsporulated oocysts and sexual stages (both macro- and microgamonts) in varying stages of development of an unidentified coccidium in both lactating and non lactating mammary glands. Gamonts developed in the alveoli of the mammary glands, and oocysts were found in the lactiferous ducts and in pools of milk. Mature macrogamonts were 11.9 × 15.2 μm (10-14 × 14-20 μm), whereas completely developed microgamonts with many gametes were 14.8 × 16.8 μm (10-18 × 13-20 μm). Oocysts in tissue sections …

Eimeria Species From Cryptotis Shrews (Insectivora: Soricidae) With Description Of A New Species, Donald W. Duszynski, Ralph P. Eckerlin, Timothy J. Mccarthy

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Fresh fecal samples from 12 shrews, 10 Cryptotis goodwini Jackson, 1933, and 2 Cryptotis merriami Thomas, 1898, were collected between December 1994 and May 1998; 11 (92%) were positive for eimeriid coccidia, including 8 of 8 C. goodwini and 2 of 2 C. merriami from Honduras and 1 of 2 C. goodwini from Guatemala. Two Eimeria species were found: 1 is consistent with the description of Eimeria whitakeri Upton and McAllister, 1991, and 1 is described in this study as new. Sporulated oocysts of the new species are subspheroidal, 16.8 × 15.7 (14–20 × 15–19) μm, with a length–width (L/W) …

Cattle Industry Biosecurity Plan, Department Of Agriculture Western Australia

Biosecurity published reports

StockGuard has been initiated to develop a series of biosecurity plans for the animal industries in Western Australia. Through StockGuard, representatives from the animal industries have been working with the Department of Agriculture Western Australian to identify potential threats to each of the animal industries and to develop action plans detailing how these threats can be managed. The overall purpose of StockGuard is to highlight threats to the animal industries before they occur and to develop effective ways to minimise the potential impact of these threats.

The recommendations of the working group are based on detailed strategic assessments of the …

Method To Detect The Presence Of A Microorganism Or Agent In An Animal, David R. Smith, Laura L. Hungerford, Rodney A. Moxley, Susanne Hinkley, Terry Klopfenstein, Jeff Gray

School of Veterinary and Biomedical Sciences: Faculty Publications

The present invention provides a method to detect the presence of a microorganism or agent in an animal. The method encompasses placement of devices at various locations where the animal resides so as to induce the animal to initiate contact with the device. As a result of this contact, the animal deposits various microorganisms and agents on the device. The device is then tested for the presence of the particular microorganism or agent of interest.

Mucosal Priming Of Simian Immunodeficiency Virus-Specific Cytotoxic T-Lymphocyte Responses In Rhesus Macaques By The Salmonella Type Iii Secretion Antigen Delivery System, David T. Evans, Li-Mei Chen, Jacqueline Gillis, Kuei-Chin Lin, Brian Harty, Gail P. Mazzara, Ruben O. Donis, Keith G. Mansfield, Jeffrey D. Lifson, Ronald C. Desrosiers, Jorge E. Galán, R. Paul Johnson

School of Veterinary and Biomedical Sciences: Faculty Publications

Nearly all human immunodeficiency virus (HIV) infections are acquired mucosally, and the gut-associated lymphoid tissues are important sites for early virus replication. Thus, vaccine strategies designed to prime virus-specific cytotoxic T lymphocyte (CTL) responses that home to mucosal compartments may be particularly effective at preventing or containing HIV infection. The Salmonella type III secretion system has been shown to be an effective approach for stimulating mucosal CTL responses in mice. We therefore tested ∆phoP-phoQ attenuated strains of Salmonella enterica serovar Typhimurium and S. enterica serovar Typhi expressing fragments of the simian immunodeficiency virus (SIV) Gag protein fused to the …

Eimeria Species From Serows (Capricornis Spp.) In Japan With Descriptions Of Two New Species, Lee Couch, Shigehiko Uni, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Fresh fecal samples from 35 Japanese serows Capricornis crispus (Temminck, 1845), and 5 Formosan serows Capricornis swinhoei Gray, 1862, were collected between October 1998 and March 1999. Eimeriid coccidians were found in 12 (34%) Japanese serows and in 1 (20%) Formosan serow. Three Eimeria species were found, and 2 are described here as new. The third species is consistent with the description of Eimeria kamoshika Inoue, 1989. Sporulated oocysts of 1 new species are broadly ellipsoidal, 20.7 × 16.8 μm (18–23 × 14–19 μm) with a length (L):width (W) ratio of 1.2 (1.1–1.4); these lack a micropyle (M) and oocyst …

Taenia Pencei N. Sp. From The Ringtail, Bassariscus Astutus (Carnivora: Procyonidae), In Texas, U.S.A., Robert L. Rausch

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Taenia pencei n. sp. is described from the ringtail, Bassariscus astutus (Carnivora: Procyonidae), in western Texas, U.S.A., and its metacestode is characterized on the basis of specimens from Peromyscus cf. maniculatus in southern Oregon. Taenia pencei n. sp. apparently occurs exclusively in the ringtail, and it is the first cestode of the genus Taenia known from a member of the family Procyonidae. It is morphologically most similar to species of Taenia from mammals of the family Mustelidae in subclade two of the recently constructed phylogeny of that genus. Taenia pencei is distinguished from the nearctic Taenia taxidiensis in the badger …

Elaphrobates Euzeti Gen. And Sp. N. (Digenea: Sanguinicolidae) From Snappers (Lutjanidae) In The Gulf Of Mexico, Stephen A. Bullard, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Elaphrobates euzeti gen. and sp. n. (Digenea: Sanguinicolidae) infects the heart and branchial vessels of the red snapper (Lutjanus campechanus) and the heart of the gray snapper (Lutjanus griseus) in the Gulf of Mexico. It has ventrolateral tegumental spine rows each consisting of 4-7 spines, an oral sucker with 6-7 concentric spine rows, sinuous posterior ceca extending to the ovary and 2-4 times longer than the anterior ceca, a single mostly intercecal testis not extending beyond the lateral nerve cords, a post-testicular ovary, a dextral ootype at or immediately anterior to level of the female pore, …