Immunology and Infectious Disease Commons^™

Osmotic Stress, Not Aldose Reductase Activity, Directly Induces Growth Factors And Mapk Signaling Changes During Sugar Cataract Formation, Peng Zhang, Kuiyi Xing, James Randazzo, Karen Blessing, Marjorie F. Lou, Peter Kador

School of Veterinary and Biomedical Sciences: Faculty Publications

In sugar cataract formation in rats, aldose reductase (AR) actitvity is not only linked to lenticular sorbitol (diabetic) or galactitol (galactosemic) formation but also to signal transduction changes, cytotoxic signals and activation of apoptosis. Using both in vitro and in vivo techniques, the interrelationship between AR activity, polyol (sorbitol and galactitol) formation, osmotic stress, growth factor induction, and cell signaling changes have been investigated. For in vitro studies, lenses from Sprague Dawley rats were cultured for up to 48 hrs in TC-199-bicarbonate media containing either 30 mM fructose (control), or 30 mM glucose or galctose with/without the aldose reductase inhibitors …

Heterogeneous Nuclear Ribonucleoprotein K Supports Vesicular Stomatitis Virus Replication By Regulating Cell Survival And Cellular Gene Expression, Phat X. Dinh, Anshuman Das, Rodrigo Franco, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

The heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a member of the family of hnRNPs and was recently shown in a genome-wide small interfering RNA (siRNA) screen to support vesicular stomatitis virus (VSV) growth. To decipher the role of hnRNP K in VSV infection, we conducted studies which suggest that the protein is required for VSV spreading. Virus binding to cells, entry, and nucleocapsid uncoating steps were not adversely affected in the absence of hnRNP K, whereas viral genome transcription and replication were reduced slightly. These results indicate that hnRNP K is likely involved in virus assembly and/or release from …

Characterization Of The Gut Microbiota And Colitogenic Bacterial Species In Core 1 O-Glycans Deficient Mice, Maria E. Perez-Munoz

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Mucus is one important component of the intestinal mucosal barrier, and loss of its functionality is associated with colitis in humans and mice. Mice deficient in core 1 O-glycans (TM-IEC C1galt1^-/-) display a thinner mucus layer upon mutation of the glycosyltransferase enzyme (C1GALT1) responsible for core 1 O-glycans attachment, thus constituting an experimental model for human Ulcerative Colitis (UC). Under conventional conditions, these mice experience spontaneous inflammation of the colon. However, the exact role of the microbiota in colitis development in these mice has not been systematically investigated.

Aimed to gain insights into the role of …

Ectoparasites Of The Virginia Opossum (Didelphis Virginiana), Raccoon (Procyon Lotor), And Striped Skunk (Mephitis Mephitis) From Keith County, Nebraska, Lance A. Durden, Dennis J. Richardson

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Six Virginia opossums (Didelphis virginiana), nine raccoons (Procyon lotor) and one striped skunk (Mephitis mephitis) collected from Keith County Nebraska were examined for ectoparasites. All three host species were parasitized by adults of the American dog tick, Dermacentor variabilis. Opossums were also parasitized by the flea Pulex simulans and the tiny fur mite Didelphilichus serri­fer; the latter species represents a new state record for Nebraska. Raccoons were also parasitized by P. simulans and by the lago­morph-associated flea Euhoplopsyllus glacialis affinis, whereas the skunk was also parasitized by the chewing louse Neotrichodectes …

Estudios Científicos En El Estado De Hidalgo Y Zonas Aledañas, Volumen Ii, Griselda Pulido-Flores, Scott Monks

Zea E-Books Collection

Los 16 capítulos que conforman este libro son una contribución al conocimiento de la biodiversidad y conservación de los recursos naturales en el estado de Hidalgo, México.

Se aborda el derecho de propiedad intelectual en la biodiversidad, citando algunos ejemplos de México. Así mismo, se presenta un estudio de la biodiversidad y distribución de la herpetofauna en cuatro tipos de vegetación. Desde el punto de vista de la helmintología se presentan dos estudios de los helmintos parásitos de vertebrados silvestres; con nuevos registros de hospederos y localidades de algunas especies de helmintos para Hidalgo. También, se aborda el uso de …

Mycobacterium Bovis (Bovine Tuberculosis) Infection In North American Wildlife: Current Status And Opportunities For Mitigation Of Risks Of Further Infection In Wildlife Populations, Ryan S. Miller, Steven J. Sweeney

Zoonotics and Wildlife Disease: Publications

Mycobacterium bovis (M. bovis), the causative agent of bovine tuberculosis, has been identified in nine geographically distinct wildlife populations in North America and Hawaii and is endemic in at least three populations, including members of the Bovidae, Cervidae, and Suidae families. The emergence of M. bovis in North American wildlife poses a serious and growing risk for livestock and human health and for the recreational hunting industry. Experience in many countries, including the USA and Canada, has shown that while M. bovis can be controlled when restricted to livestock species, it is almost impossible to eradicate …

Diseases At The Livestock–Wildlife Interface: Status, Challenges, And Opportunities In The United States, Ryan S. Miller, Mathew L. Farnsworth, Jennifer L. Malmberg

Zoonotics and Wildlife Disease: Publications

In the last half century, significant attention has been given to animal diseases; however, our understanding of disease processes and how to manage them at the livestock–wildlife interface remains limited. In this study, we conduct a systematic review of the scientific literature to evaluate the status of diseases at the livestock–wildlife interface in the United States. Specifically, the goals of the literature review were three fold: first to evaluate domestic animal diseases currently found in the United States where wildlife may play a role; second to identify critical issues faced in managing these diseases at the livestock–wildlife interface; and third …

American Society Of Parasitologists Newsletter, V. 35, No. 1, Spring 2013, Scott Lyell Gardner

American Society of Parasitologists: Newsletter

American Society of Parasitologists Newsletter, volume 35, number 1, Spring 2013

The Herpesvirus Vp1/2 Protein Is An Effector Of Dynein-Mediated Capsid Transport And Neuroinvasion, Sofia V. Zaichick, Kevin P. Bohannon, Ami Hughes, Patricia J. Sollars, Gary E. Pickard, Gregory A. Smith

School of Veterinary and Biomedical Sciences: Faculty Publications

Microtubule transport of herpesvirus capsids from the cell periphery to the nucleus is imperative for viral replication and, in the case of many alphaherpesviruses, transmission into the nervous system. Using the neuroinvasive herpesvirus, pseudorabies virus (PRV), we show that the viral protein 1/2 (VP1/2) tegument protein associates with the dynein/dynactin microtubule motor complex and promotes retrograde microtubule transport of PRV capsids. Functional activation of VP1/2 requires binding to the capsid protein pUL25 or removal of the capsid-binding domain. A proline-rich sequence within VP1/2 is required for the efficient interaction with the dynein/ dynactin microtubule motor complex as well as for …

Pathology In Practice, Timothy L. Cushing, David J. Steffen, Gerald E. Duhamel

School of Veterinary and Biomedical Sciences: Faculty Publications

A 1.5-year-old second-parity Large Black X Tamworth cross sow from a well-managed 20-sow, unvaccinated, pasture-raised herd of pigs in upstate New York aborted a litter of 7 variably mummified near-term fetuses and stillborn piglets. This sow had no signs of ill health other than abortion; it was housed in a group with 4 other sows and a 2-year-old Gloucestershire Old Spot boar. One of the stillborn piglets from this litter was submitted to the New York State Animal Health Diagnostic Center for necropsy; no placental tissue was submitted with the piglet. A second sow from this group had aborted a …

Review Of Haploporid (Trematoda) Genera With Ornate Muscularization In The Region Of The Oral Sucker, Including Four New Species And A New Genus, Eric E. Pulis, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Species of the Haploporidae Nicoll, 1914 with elaborate muscularization of the oral sucker belong in three trematode genera, including three new species and a new genus from the intestine of fishes in Australian waters. Spiritestis Nagaty, 1948 is resurrected and S. herveyensis n. sp. is described from the mullet Moolgarda seheli (Forsskål) collected in Hervey Bay, Queensland, Australia; the latter differs from S. arabii Nagaty, 1948 in that the position of the genital pore is pharyngeal rather than post-pharyngeal and the geographical range is off Australia rather than the Red Sea. A new genus is proposed for two new species, …

Title Page, Contents, Contributors For Estudios Científicos En El Estado De Hidalgo Y Zonas Aledañas, Volumen Ii, Griselda Pulido-Flores, Scott Monks

Estudios científicos en el estado de Hidalgo y zonas aledañas

Title Page

Contents

Contributors

Los Parásitos Y El Estudio De Su Biodiversidad: Un Enfoque Sobre Los Estimadores De La Riqueza De Especies, Christian E. Bautista-Hernández, Scott Monks, Griselda Pulido-Flores

Estudios científicos en el estado de Hidalgo y zonas aledañas

Durante años los parásitos fueron considerados únicamente como agentes patógenos que debían ser eliminados, sin considerar que son un componente importante de la biodiversidad, que son capaces de aportar información de sí mismos y de otros organismos. Existen estimadores de riqueza que predicen el número de especies para una comunidad a partir de la riqueza observada y de la abundancia de las especies o datos de incidencia; pueden ser estimadores paramétricos y no paramétricos. La comparación de la riqueza de especies entre componente de comunidades de parásitos frecuentemente pueden ser difícil de evaluar debido a que los datos no se …

El Uso De Helmintos Parásitos Como Bioindicadores En La Evaluación De La Calidad Del Agua: Lago De Tecocomulco Vs. Laguna De Metztitlán, Hidalgo, México, Scott Monks, Griselda Pulido-Flores, Christian E. Bautista-Hernández, Berenice Alemán-García, Jorge Falcón-Ordaz, Juan Carlos Gaytán-Oyarzún

Estudios científicos en el estado de Hidalgo y zonas aledañas

Como parte integral de la evaluación de la calidad ambiental del estado de Hidalgo, este estudio tiene énfasis en los helmintos parásitos de los peces y su uso como bioindicadores de la calidad de agua en Lago de Tecocomulco y Laguna de Metztitlán. Como parte del estudio, se obtuvieron los parámetros ecológicos de la infección para cada especie. En Lago Tecocomulco se registraron dos especies de digéneos (larvas), Posthodiplostomum sp. y Diplostomum sp. en el cerebro, los ojos y el mesenterio de dos especies de peces nativos de la zona (Chirostoma jordani y Girardinicthys viviparus); un céstodo (larva), …

Helmintos Parásitos De Algunos Roedores (Mammalia: Rodentia) En San Miguel De Allende, Tepeapulco, Hidalgo, México, Griselda Pulido-Flores, Scott Monks, Jorge Falcón-Ordaz

Estudios científicos en el estado de Hidalgo y zonas aledañas

Los helmintos son un componente de la biodiversidad, que proveen información sobre la historia natural de los hospederos, y de las relaciones tróficas. El conocimiento de la diversidad de roedores y sus parásitos en Hidalgo es escaso y puntual. Por la amplia distribución de los roedores, su adaptabilidad a los ambientes modificados y por el riesgo zoonótico que representan, es importante conocer la parasitofauna de estos hospederos. El objetivo del este trabajo fue registrar la helmintofauna de algunos roedores en San Miguel de Allende, Tepeapulco, Hidalgo, México. Entre agosto de 2005 y junio de 2007 se colectaron 19 roedores (12 …

Análisis Morfométrico De Glypthelmins Quieta (Stafford, 1900) Stafford, 1905 (Digenea: Macroderoididae) En La Reserva De La Biosfera Barranca De Metztitlán, Hidalgo, México, Francisco Alemán-Muñoz, Griselda Pulido-Flores, Scott Monks, Jorge Falcón-Ordaz

Estudios científicos en el estado de Hidalgo y zonas aledañas

Se evaluó la variación morfológica de 30 ejemplares adultos de la especie Glypthelmins quieta, parásitos de Lithobates spectabilis en localidad de Metznoxtla, en la Reserva de la biosfera Barranca de Metztitlán, Hidalgo. El objetivo del presente trabajo fue contribuir al conocimiento de la variación morfológica de G. quieta, y evaluar si los caracteres de presencia ausencia de glándulas perifaringeas y forma de las glándulas mediales son validos para la asignación de ejemplares a una especie. Se generó una base de datos morfológicos realizando tres análisis de componentes principales (ACP). El primer ACP se realizó con la totalidad de …

Análisis De Metales Pesados En Un Céstodo De La Familia Dilepididae, Jorge Falcón-Ordaz, Scott Monks, Griselda Pulido-Flores, Claudia Romo-Gómez

Estudios científicos en el estado de Hidalgo y zonas aledañas

La concentración de plomo (Pb), cadmio (Cd), zinc (Zn), níquel (Ni), hierro (Fe) y aluminio (Al) fue determinada en un céstodo de la familia Dilepididae, parásito de Peromyscus difficilis colectados en una zona rural del Estado de México; con respecto a trabajos previos se obtuvieron valores altos de concentración para Pb y Zn mientras que para Ni sus valores estuvieron por debajo de los valores reportados y el Cd no fue registrado, en lo que respecta al Fe y Al no existen registros de estos metales en céstodos parásitos de organismos terrestres. Con los datos registrados en el presente trabajo …

Nemátodos Parásitos De Roedores De Huehuetla, Hidalgo, México, Jorge Falcón-Ordaz, Scott Monks, Griselda Pulido-Flores

Estudios científicos en el estado de Hidalgo y zonas aledañas

El estudio de nemátodos parásitos de roedores en el estado de Hidalgo es prácticamente desconocido, solamente se han registrado para la entidad un total de 17 especies. Es por esto que se llevó a cabo una recolecta de roedores silvestres en el municipio de Huehuetla, Hidalgo, con el objetivo de conocer los helmintos que parasitan dichos hospederos. Los hospederos se capturaron con trampas Sherman para su posterior sacrificio y revisión helmintológica. Se registra por primera vez Syphacia sp. en Peromyscus mexicanus (Saussure, 1860) como nuevo hospedero. Se ratifica la identificación de Trichuris fossor Hall, 1916 y Hassalstrongylus aduncus Chandler, 1932; …

Rhodotorula Minuta Fungemia In A Ewe Lamb, C. G. Chitko-Mckown, K. A. Leymaster, M. P. Heaton, D. D. Griffin, J. K. Veatch, S. A. Jones, M. L. Clawson

School of Veterinary and Biomedical Sciences: Faculty Publications

An 8-month-old crossbred ewe, normal upon physical examination, was humanely euthanized for tissue collection. After approximately 3 weeks in tissue culture, fungi began budding out of cells obtained from the choroid plexus. After an additional 3 weeks, budding was observed in kidney cell cultures and eventually in monocyte cultures as well. Serum from the lamb was submitted to the Veterinary Diagnostic Laboratory at Colorado State University for fungal diagnosis and was found negative for Aspergillus, Blastomyces, Coccidioidomycosis and Histoplasmosis. DNA was isolated from fungi collected from tissue culture supernatants and used in a set of pan-fungal PCR assays with …

Bovine Herpes Virus 1 (Bhv-1) And Herpes Simplex Virus Type 1 (Hsv-1) Promote Survival Of Latently Infected Sensory Neurons, In Part By Inhibiting Apoptosis, Clinton Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

α-Herpesvirinae subfamily members, including herpes simplex virus type 1 (HSV-1) and bovine herpes virus 1 (BHV-1), initiate infection in mucosal surfaces. BHV-1 and HSV-1 enter sensory neurons by cell-cell spread where a burst of viral gene expression occurs. When compared to non-neuronal cells, viral gene expression is quickly extinguished in sensory neurons resulting in neuronal survival and latency. The HSV-1 latency associated transcript (LAT), which is abundantly expressed in latently infected neurons, inhibits apoptosis, viral transcription, and productive infection, and directly or indirectly enhances reactivation from latency in small animal models. Three anti-apoptosis genes can be substituted for LAT, which …

First Descriptions Of Dicyemid Mesozoans (Dicyemida: Dicyemidae) From Australian Octopus (Octopodidae) And Cuttlefish (Sepiidae), Including A New Record Of Dicyemennea In Australian Waters, Sarah R. Catalano

Harold W. Manter Laboratory of Parasitology: Library Materials

Three new species of dicyemid mesozoans are described for the first time from Australian octopus and cuttlefish species. Dicyemennea floscephalum sp. n. is described from Octopus berrima Stranks et Norman (southern keeled octopus) collected from Spencer Gulf and Gulf St. Vincent, South Australia, Australia and represents the first description of a species of Dicyemennea Whitman, 1883 from Australian waters. Dicyema papuceum sp. n. and D. furuyi sp. n. are described from Sepia papuensis Hoyle (Papuan cuttlefish) collected from Shark Bay, Western Australia, Australia. Dicyemennea floscephalum sp. n. is a medium to large species that reaches approximately 4.9 mm in …

Manipulation Of Cellular Processing Bodies And Their Constituents By Viruses, Asit K. Pattnaik, Phat X. Dinh

School of Veterinary and Biomedical Sciences: Faculty Publications

The processing bodies (PBs) are a form of cytoplasmic aggregates that house the cellular RNA decay machinery as well as many RNA-binding proteins and mRNAs. The PBs are constitutively present in eukaryotic cells and are involved in maintaining cellular homeostasis by regulating RNA metabolism, cell signaling, and survival. Virus infections result in modification of the PBs and their constituents. Many viruses induce compositionally altered PBs, while many others use specific components of the PBs for their replication. PB constituents are also known to restrict virus replication by a variety of mechanisms. Further, continuing studies in this rapidly emerging field of …

Deep Sequencing And Genome-Wide Analysis Reveals The Expansion Of Microrna Genes In The Gall Midge Mayetiola Destructor, Chitvan Khajuria, Christie E. Williams, Mustapha El Bouhssini, R. Jeff Whitworth, Stephen Richards, Jeffrey J. Stuart, Ming-Shun Chen

School of Veterinary and Biomedical Sciences: Faculty Publications

Background: MicroRNAs (miRNAs) are small non-coding RNAs that play critical roles in regulating post transcriptional gene expression. Gall midges encompass a large group of insects that are of economic importance and also possess fascinating biological traits. The gall midge Mayetiola destructor, commonly known as the Hessian fly, is a destructive pest of wheat and model organism for studying gall midge biology and insect – host plant interactions.

Results: In this study, we systematically analyzed miRNAs from the Hessian fly. Deep-sequencing a Hessian fly larval transcriptome led to the identification of 89 miRNA species that are either identical or very …

A Description Of Neoechinorhynchus (Neoechinorhynchus) Veropesoi N. Sp. (Acanthocephala: Neoechinorhynchidae) From The Intestine Of The Silver Croaker Fish Plagioscion Squamosissimus (Heckel, 1840) (Osteichthyes: Sciaenidae) Off The East Coast Of Brazil, Francisco Tiago De Vasconcelos Melo, P. A.F.B. Costa, E. G. Giese, Scott Lyell Gardner, J. N. Santos

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Plagioscion squamosissimus (Heckel, 1840) (Osteichthyes: Sciaenidae) is considered piscivorous and is a generalist species endemic to the Amazon region. This fish is an important part of the natural ecosystems in which it occurs and provides basic functional components in the food web. The genus Neoechinorhynchus Stiles & Hassall, 1905 is distributed worldwide and parasitizes fish and turtles, but there are few reports of parasites of this genus in South America, due to the high diversity of fish that can be found in this region. A new species of thorny-headed worm (Acanthocephala: Neoechinorhynchidae) is described from P. squamosissimus from Guajará Bay, …

Paleoparasitology: The Origin Of Human Parasites; Paleoparasitologia: A Origem Dos Parasitas Humanos, Adauto Araújo, Karl Reinhard, Luis Fernando Ferreira, Elisa Pucu, Pedro Paulo Chieffi

Karl Reinhard Publications

Parasitism is composed by three subsystems: the parasite, the host, and the environment. There are no organisms that cannot be parasitized. The relationship between a parasite and its host species most of the time do not result in damage or disease to the host. However, in a parasitic disease the presence of a given parasite is always necessary, at least in a given moment of the infection. Some parasite species that infect humans were inherited from pre-hominids, and were shared with other phylogenetically close host species, but other parasite species were acquired from the environment as humans evolved. Human migration …

Worms, Nematoda, Scott Lyell Gardner

Scott L. Gardner Publications

Nematodes are the most speciose phylum of metazoa on earth. Not only do they occur in huge numbers as parasites of all known animal groups, but also they are found in the soils, as parasites of plants, and in large numbers in the most extreme environments, from the Antarctic dry valleys to the benthos of the ocean. They are extremely variable in their morphological characteristics, with each group showing morphological adapta­tions to the environment that they inhabit. Soil-dwelling forms are extremely small; many marine species have long and complex setae; and parasitic species man­ifest amazingly great reproductive potential and large …

Influence Of Therapeutic Ceftiofur Treatments Of Feedlot Cattle On Fecal And Hide Prevalences Of Commensal Escherichia Coli Resistant To Expanded-Spectrum Cephalosporins, And Molecular Characterization Of Resistant Isolates, John W. Schmidt, Dee Griffin, Larry A. Kuehn, Dayna M. Brichta-Harhay

School of Veterinary and Biomedical Sciences: Faculty Publications

In the United States, the bla_CMY-2 gene contained within incompatibility type A/C (IncA/C) plasmids is frequently identified in extended-spectrum-cephalosporin-resistant (ESC^r) Escherichia coli strains from both human and cattle sources. Concerns have been raised that therapeutic use of ceftiofur in cattle may increase the prevalence of ESC^r E. coli. We report that herd ESC^r E. coli fecal and hide prevalences throughout the residency of cattle at a feedlot, including during the period of greatest ceftiofur use at the feedlot, were either not significantly different (P≥0.05) or significantly less (Pr E. coli shedding that follows ceftiofur …

Complete Closed Genome Sequences Of Mannheimia Haemolytica Serotypes A1 And A6, Isolated From Cattle, Gregory P. Harhay, Sergey Koren, Adam M. Phillippy, D. Scott Mcvey, Jennifer Kuszak, Michael L. Clawson, Dayna M. Harhay, Michael P. Heaton, Carol G. Chitko-Mckown, Timothy P. L. Smith

School of Veterinary and Biomedical Sciences: Faculty Publications

Mannheimia haemolytica is a respiratory pathogen affecting cattle and related ruminants worldwide. M. haemolytica is commonly associated with bovine respiratory disease complex (BRDC), a polymicrobial multifactorial disease.Wepresent the first two complete closed genome sequences of this species, determined using an automated assembly pipeline requiring no manual finishing.

Development And Evaluation Of A Replicon Particle Vaccine Expressing The E2 Glycoprotein Of Bovine Viral Diarrhea Virus (Bvdv) In Cattle, John Dustin Loy, Jill Gander, Mark Mogler, Ryan Vander Veen, Julia Ridpath, Delbert Hank Harris, Kurt Kamrud

School of Veterinary and Biomedical Sciences: Faculty Publications

Background: Bovine viral diarrhea virus is one of the most significant and costly viral pathogens of cattle worldwide. Alphavirus-derived replicon particles have been shown to be safe and highly effective vaccine vectors against a variety of human and veterinary pathogens. Replicon particles are non-propagating, DIVA compatible, and can induce both humoral and cell mediated immune responses. This is the first experiment to demonstrate that Alphavirus-based replicon particles can be utilized in a standard prime/boost vaccination strategy in calves against a commercially significant bovine pathogen.

Findings: Replicon particles that express bovine viral diarrhea virus sub-genotype 1b E2 glycoprotein were …

A Dysfunctional Tricarboxylic Acid Cycle Enhances Fitness Of Staphylococcus Epidermidis During Β-Lactam Stress, Vinai Chittenzham Thomas, Lauren Kinkhead, Ashley Janssen, Carolyn Schaeffer, Keith Woods, Jill Lindgren, Johnathan Peaster, Sujata Chaudhari, Marat Sadykov, Joselyn Jones, Sameh Mohamadi Abdelghani, Matthew Zimmerman, Kenneth W. Bayles, Greg Somerville, Paul Fey

School of Veterinary and Biomedical Sciences: Faculty Publications

A recent controversial hypothesis suggested that the bactericidal action of antibiotics is due to the generation of endogenous reactive oxygen species (ROS), a process requiring the citric acid cycle (tricarboxylic acid [TCA] cycle). To test this hypothesis, we assessed the ability of oxacillin to induce ROS production and cell death in Staphylococcus epidermidis strain 1457 and an isogenic citric acid cycle mutant. Our results confirm a contributory role for TCA-dependent ROS in enhancing susceptibility of S. epidermidis toward β-lactam antibiotics and also revealed a propensity for clinical isolates to accumulate TCA cycle dysfunctions presumably as a way to tolerate these …