Immunology and Infectious Disease Commons^™

Enterobius Vermicularis Ancient Dna In Pre-Columbian Human Populations, Alena Mayo Iniguez, Karl J. Reinhard, Luiz Fernando Ferreira, Adauto Araujo, Ana Carolina, Paulo Vincente

Karl Reinhard Publications

In prehistoric populations the paleoparasitological findings show an Enterobius vennicularis homogeneous distribution among North American hunter-gatherers intensified with the advent of agriculture. The same occurred in the transition from nomad hunter-gatherers to sedentary farmers in South America, although E. vermicularis infection encompasses only the ancient Andean peoples. Since molecular techniques are sensitive in detecting ancient DNA (aDNA), in this work we have performed a molecular paleoparasitological study of E. vermicularis. aDNA was recovered from North and South American coprolites (4110 BC-AD 900). Human (cox 2 and HVR) and pinworm (5S rRNA spacer) sequences were determined. The sequence analysis confirmed E. …

Pathoecology And The Future Of Coprolite Studies In Bioarchaeology, Karl J. Reinhard, Vaughn M. Bryant Jr.

Karl Reinhard Publications

Human coprolites currently provide an expanding array of information about the diet, health, and ecology of prehistoric people in the Southwest, but for many years coprolites were not recognized or preserved, or they were not considered important and thus were not saved (Bryant and Dean 2006). With the expansion of archaeological field work during the last half of the twentieth century archaeologists have increasingly explored the "complete" potentials of sites, including the collection and analysis of geomorphologic, botanical, and faunal data. In some ideal habitats (e.g., very dry or frozen) this includes exploring the scientific potential of human coprolite studies. …

A Child And His Fate: Tuberculosis, Perimortem Trauma And Mummification, Sheila M. M.De Souza, Karl J. Reinhard, Bernardo Tessarollo, Jose Fernando Cardona Zannier, Adauto Araujo

Karl Reinhard Publications

A male child, sitting, 7 years, circumferential deformation, skin marked by coiled fibers, nostril plugs, consistent with Aymara practices was CT scanned. Puppae in the skin suggest exposition of the body previous to the funeral. Dark brown color at the skin suggest blood perfusion. A broken area at the right frontal with the skin partially inside is suggestive of perimorten trauma. There were no bone debris inside the skull and the CT images show two confluent fractures defined by radiating lines extending beyond the missing bone surface. The right eyeball is projected out, the tongue is outside the mouth, the …

Pathoecology Of Two Ancestral Pueblo Villages, Karl Reinhard

Karl Reinhard Publications

Pathoecology is the study of the biotic, abiotic, and cultural environments of disease (Martinson et al. 2003). A parasitic infection is the result of the pathoecological interaction of host behavior, parasite life cycle, the environment in which both life forms live, the nutritional status of the host, and host physiological responses to all of these factors. Parasites contribute to anemia in many ways. Some, such as hookworm, actually consume blood and cause iron loss through their activities. For other parasites, symptoms such as profuse diarrhea reduce intestinal absorption of nutrients. Others, such as certain fish tapeworms, actually compete for absorption …

Down-Regulation Of Natural Killer Cell Activation In Response To Influenza Virus In Older Adults, Yu Jing

Theses and Dissertations in Biomedical Sciences

Immune senescence contributes to influenza-associated high mortality and morbidity and reduced vaccine efficacy in elderly people. Type I T cell (Thl)-mediated immunity plays a significant role in Immune responses to influenza infection and vaccination. Natural killer (NK) cells secrete significant amount of IFN-7 , a hallmark Thl cytokine, in response to influenza infection. How aging influences human NK cell IFN-7 production in response to influenza virus has not been well documented. In this study we employed human peripheral blood mononuclear cells (PBMC) and performed intracellular cytokine staining and flow cytometry primarily to investigate how aging influences NK cell activation with …

Grânulos De Amido E Fitólitos Em Cálculos Dentários Humanos: Contribuição Ao Estudo Do Modo De Vida E Subsistência De Grupos Sambaquianos Do Litoral Sul Do Brasil, Verônica Wesolowski, Sheila Maria Ferraz Mendonça De Souza, Karl Reinhard, Gregório Ceccantini

Karl Reinhard Publications

Este artigo tem por objetivo apresentar os resultados da recuperação e análise de micro-vestígios vegetais retidos em cálculos dentários de grupos de pescadores-coletores do litoral sul do Brasil. Apresenta também os resultados das relações mantidas entre grânulos de amido e cárie, e entre fitólitos e desgaste dentário.

Através da dissolução química de cálculos dentários foram recuperados microvestígios vegetais em todos os indivíduos estudados. Foi evidenciado o consumo de alimentos amiláceos em todas as séries esqueléticas estudadas, demonstrando independência entre dieta com aporte de produtos vegetais e utilização de cerâmica. Foram encontrados resultados sugestivos de padrões de escolha de alimentos diferenciados …

A Coprological View Of Ancestral Pueblo Cannibalism, Karl Reinhard

Karl Reinhard Publications

As the object of my scientific study, I’ve chosen coprolites. It’s not a common choice, but to a paleonutritionist and archaeoparasitologist, a coprolite—a sample of ancient feces preserved by mineralization or simple drying—is a scientific bonanza. Analysis of coprolites can shed light on both the nutrition of and parasites found in prehistoric cultures. Dietary reconstructions from the analysis of coprolites can inform us about, for example, the origins of modern Native American diabetes. With regard to parasitology; coprolites hold information about the ancient emergence and spread of human infectious disease. Most sensational, however, is the recent role of coprolite analysis …

The Grazing Of Sheep In The Pastoral Areas Of Western Australia : Best Management Practice Guidelines, Department Of Agriculture And Food, Western Australia

Land resources best practice series

The Land Administration Act 1997 requires a pastoralist to manage and work the land within a pastoral lease to its best advantage and use methods of best pastoral and environmental management practices to achieve sustainable land use. The Pastoral Lands Board will use these best practices as a guideline.

Sl1 Rna Gene Recovery From Enterobius Vermicularis Ancient Dna In Pre-Columbian Human Coprolites, Alena M. Iñiguez, Karl Reinhard, Marcelo Luiz Carvalho Gonçalves, Luiz Fernando Ferreira, Adauto Araújo, Ana Carolina Paulo Vincente

Karl Reinhard Publications

Enterobius vermicularis, pinworm, is one of the most common helminths worldwide, infecting nearly a billion people at all socio-economic levels. In prehistoric populations the paleoparasitological findings show a pinworm homogeneous distribution among hunter-gatherers in North America, intensified with the advent of agriculture. This same increase also occurred in the transition from nomad hunter-gatherers to sedentary farmers in South America, although E. vermicularis infection encompasses only the ancient Andean peoples, with no record among the pre-Colombian populations in the South American lowlands. However, the outline of pinworm paleo epidemiology has been supported by microscopic finding of eggs recovered from coprolites. …

How Many Years Of Life Could Be Saved If Malaria Were Eliminated From A Hyperendemic Area Of Northern Ghana?, Ayaga A. Bawah, Fred N. Binka

Poverty, Gender, and Youth

For some time, Chinese government policies have treated rural and urban areas very differently, and a by-product of China’s rapid development seems to be an even greater differentiation between urban and rural social and economic life. Over the next several decades, in part because of rapid fertility declines and in part as a result of mortality declines at older ages, China and other developing countries will experience enormous increases in the proportion of older adults and the proportion of the “oldest-old.” It is reasonable to expect that these age structure changes will alter the provision of health care, making an …

The Southwestern Association Of Parasitologists: The First 35 Years: 1967–2002, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

First two paragraphs:

It is likely that the idea to organize southwestern parasitologists into a regional group originated over cocktails one night in the late 1950s during a regional or national meeting. What is clear is that two men—Drs. Robert E. Kuntz and Donald V. Moore— were instrumental in turning the idea into what has become, in reality, the Southwestern Association of Parasitologists (SWAP).

Dr. J. Teague Self (personal communication, 1981) stated, “The beginning of SWAP was an idea of Robert Kuntz who felt that something could be gained if several of us here in the southwest could get together …

The Relationship Between Health Status And Hope In Individuals With Hiv Disease, Celine Meera Chohan

All-Inclusive List of Electronic Theses and Dissertations

This study examined the relationship between hope and health status over a two-year period in individuals who were HIV -infected. One hundred and three individuals completed the surveys at entry into the study, at 12 months, and at 24 months. The Health Status Questionnaire (Wu et.al., 1991) measured Cognitive Functioning, Emotional Functioning, and Physical Functioning. Additionally, the Hope Scale (Snyder, 1991) and the Client Information Form were used to collect data. Results suggested hope at entry into the study was a significant predictor of physical functioning initially, at 12 months, and at 24 months. This finding suggests a link between …

The Benefits Of Breastfeeding: An Introduction For Health Educators, Sheila G. J. Clark, Timothy J. Bungum

Environmental & Global Health Faculty Research

Currently 16% of Americans breastfeed their children for at least 12 months as recommended by the AAP, which is well below the HP 2010 goal of 25%. Breastfed infants receive benefits that can improve their health throughout their lives. The benefits of breastfeeding for children include increased resistance to infectious diseases, such as gastroenteritis, respiratory tract infections, and ear infections. Breastfed children also display lower rates of chronic diseases including diabetes, obesity, asthma, and leukemia. The choice to breastfeed results in economic benefits from lower health care costs and from reduced spending on infant formula. The Healthy People 2010 targets …

Enterobius Vermicularis: Ancient Dna From North And South American Human Coprolites, Alena M. Iñiguez, Karl J. Reinhard, Adauto Araújo, Luiz F. Ferreira, Ana Carolina P. Vicente

School of Natural Resources: Faculty Publications

A molecular paleoparasitological diagnostic approach was developed for Enterobius vermicularis. Ancient DNA was extracted from 27 coprolites from archaeological sites in Chile and USA. Enzymatic amplification of human mtDNA sequences confirmed the human origin. We designed primers specific to the E. vermicularis 5S ribosomal RNA spacer region and they allowed reproducible polymerase chain reaction identification of ancient material. We suggested that the paleoparasitological microscopic identification could accompany molecular diagnosis, which also opens the possibility of sequence analysis to understand parasite-host evolution.

Trends. Security, Stigma, And Biological Weapons, Ibpp Editor

International Bulletin of Political Psychology

This Trends article discusses the political psychology of security concerns pertaining to unclassified information relating to biological weapons and/or biosecurity and how it might be used to harm US security.

Cattle Industry Biosecurity Plan, Department Of Agriculture Western Australia

Biosecurity published reports

StockGuard has been initiated to develop a series of biosecurity plans for the animal industries in Western Australia. Through StockGuard, representatives from the animal industries have been working with the Department of Agriculture Western Australian to identify potential threats to each of the animal industries and to develop action plans detailing how these threats can be managed. The overall purpose of StockGuard is to highlight threats to the animal industries before they occur and to develop effective ways to minimise the potential impact of these threats.

The recommendations of the working group are based on detailed strategic assessments of the …

Detection Of Giardia Duodenalis Antigen In Coprolites Using A Commercially Available Enzyme-Linked Immunosorbent Assay, Marcelo Luiz Carvalho Gonçalves, Adauto Araújo, Rosemere Duarte, Joaquim Pereira Da Silva, Karl Reinhard, Françoise Bouchet, Luis Fernando Ferreira

Karl Reinhard Publications

The objective of this experiment was to assess the utility of a commercially available enzyme-linked immunosorbent assay (ELISA) kit for diagnosis of giardiasis in archaeological human remains. The kit, a monoclonal antibody assay, is used to detect the presence of Giardia-specific antigen 65 (GSA65) in human feces. We utilized the assay in ancient fecal material. The material included desiccated feces found in mummies or in archaeological sites, and sediments from latrines. A total of 83 specimens, previously examined microscopically for parasites, were examined. The ELISA detected 3 positive samples, dated to about 1200 AD, 1600 AD, and 1700 AD. …

Multidisciplinary Coprolite Analysis, Karl Reinhard, Dennis R. Danielson, Mark Daniels, Sérgio Augusto De Miranda Chaves

Karl Reinhard Publications

Coprolite analysis, as reviewed by Reinhard and Bryant (1992), contributes unique and detailed information regarding diet and parasitic disease. We present here an analysis of dietary components of coprolites from Bighorn Cave using macroscopic remains, pollen concentrations, and phytoliths. In addition, we analyzed Bighorn Cave coprolites for evidence of parasitic organisms, especially intestinal worms. Such analyses of coprolites have become important methods for reconstructing past dietary and medicinal practices. Pollen concentration and phytolith quantification techniques have recently been developed, but until this report no known attempt has been made to synthesize pollen, macroscopic, and phytolith data from a single coprolite …

Out Of Africa: Origins Of The Taenia Tapeworms In Humans, Eric P. Hoberg, Nancy L. Alkire, Alan De Queiroz, Arlene Jones

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Phylogenetic and divergence date analyses indicate that the occurrence of Taenia tapeworms in humans pre-dates the development of agriculture, animal husbandry and domestication of cattle (Bos spp.) or swine (Sus scrofa) Taeniid tapeworms in Africa twice independently colonized hominids and the genus Homo prior to the origin of modern humans. Dietary and behavioural shifts, from herbivory to scavenging and carnivory, as early Homo entered the carnivore guild in the Pliocene/Pleistocene, were drivers for host switching by tapeworms to hominids from carnivores including hyaenids and felids. Parasitological data provide a unique means of elucidating the historical ecology, foraging behavior …

Medical And Social Diseases: Can The Former Moderate The Latter?, Ibpp Editor

International Bulletin of Political Psychology

This article describes how medical disease may moderate social disease in an era of globalization.

Response To Critique Of The Claim Of Cannibalism At Cowboy Wash, Patricia M. Lambert, Banks L. Leonard, Brian R. Billman, Richard A. Marlar, Margaret E. Newman, Karl J. Reinhard

Karl Reinhard Publications

The original authors of Billman et af. (2000) are joined by three other analysts from the Cowboy Wash research team to respond to the critique of this article by Dongoske et af. (2000). Dongoske and his coauthors state that Billman et af. (2000) failed to test alternative hypotheses or to consider alternative explanations for the findings at 5MTJOOJO and similar sites. The original authors point out that alternative hypotheses were examined and rejected, leaving a violent episode of cannibalism as the most plausible explanation for the remains found at 5MTJOOJO. Dongoske et af. also question many aspects of the osteological, …

Recognition Of Treponematoses In Post Repatriation X Ray And Cd Rom Nebraska Record, Karl J. Reinhard, Bruce Rothschild, Christine Rothschild, Larry Martin

Karl Reinhard Publications

Repatriation has compromised the opportunity to directly examine skeletons and to apply new diagnostic criteria and techniques. Pre-repatriation approaches to non-metric data acquisition, must make a number of assumptions: ( I ) Phenomena must be correctly identitied and segregated; (2) Criteria for severity must be specific to the phenomena studied; and (3) As criteria for disease rewgnition may change with time, it is valuable only as long as the raw data is also recorded. As part of data preservation, x-rays and CD-ROM images were recorded for skeletons trom Nebraska sites undergoing repatriation. This report concentrates on four of them 250K …

The Role Of Mummy Studies In Paleoparasitology, Adauto Araujo, Karl J. Reinhard, Luiz Fernando Ferreira

Karl Reinhard Publications

Paieoparasitology has advance during the past decade to the status of a statistically based science focused on problems of disease ecology and geographic distribution of parasitism. For most of its development, paleoparasitology has focused on the analysis of coprolites and latrine sediments. During the past few years, mummies have been increasingly included in paleoparasitology studies. We evaluate in this paper the interpretive value of mummies relative to other sources of paleoparasitological data.

EI estudio de la paleoparasitologia ha logrado transformarse, en esta ultima decada, en una ciencia basada en la estadistica, enfocada a temas tales como la ecologia de enfermedades …

Paleopharmacology, Karl Reinhard

Karl Reinhard Publications

An emerging field devoted to the exploration of the archaeological record for evidence of medicinal plants.

In the future, archaeology will have a role in pharmaceutical research. In the late twentieth century, much of the world’s pharmaceutical research has been based on ethnographic documentation of tribally recognized medicinal plants. Once the active chemical compounds in the plants are identified, they are either extracted or synthesized for commercial use. The American film Medicine Man depicts this approach. As shown in the film, such pharmacological research is threatened by declining tribal populations with loss of traditional herbal knowledge coupled with declining biodiversity. …

Archaeoparasitology, Karl Reinhard

Karl Reinhard Publications

The field devoted to the identification of parasite remains in the archaeological record and the reconstruction of past human-parasite interactions. Parasitic disease has always been a major problem. Recent summaries of the prevalence of parasitic diseases in the world today show that there are 4.5 billion infections with all species of parasitic worm, 1 billion infections with giant intestinal roundworm (Ascaris lumbricoides), 750,000,000 infections with whipworms (Trichuris trichiura), 900,000,000 infections with hookworm (Ancylostoma duodenale and Necator americanus), 657,000,000 infections with filarial worms, 200,000,000 with blood flukes (schistosome species), and 489,000,000 infections with malaria. These infections cause between 1,590,000 to 3,130,000 …

Coprolite Analysis: The Analysis Of Ancient Human Feces For Dietary Data, Karl Reinhard

Karl Reinhard Publications

Although archaeological fieldwork is hot and dirty, the most “earthy” side of the discipline is the laboratory analysis of coprolites. Each coprolite contains the remains of one to several actual meals eaten in prehistory, and analysis of many coprolites provides a picture of ancient diet that is unique in accuracy.

The term coprolite originally referred to fossilized feces in paleontological context. In archaeology, the term broadened to refer to any formed fecal mass, including mineralized, desiccated, or frozen feces and even the intestinal contents of mummies. Coprolites contain the remains of animals (parasites) that lived in the humans, the foods …

Cytotoxic T Cell Response To Influenza Vaccination In Older Adults, Caroline R. Letter

Chemistry & Biochemistry Theses & Dissertations

Influenza infections result in activation of cellular and humoral immune responses, leading to stimulation of cytotoxic T cells (CTL) and helper T cells with subsequent viral clearance.

By using an assay of granzyme B activity, the in vivo cellular-mediated immune response of old adults was found to be significantly decreased in comparison to young adults. The effect of aging on the CTL response was further investigated via enzyme-linked immunospot technique. A significant increase in number of virus-specific T cells occurred in older adults after vaccination, although the magnitude of the CTL response decreased in comparison to young adults. These data …

Tb Or Not Tb: Disease And The Adaptiveness Of Political Borders, Ibpp Editor

International Bulletin of Political Psychology

This article describes adaptive and nonadaptive aspects of political borders at the threshold of the 21st century.

Human Dental Microwear Caused By Calcium Oxalate Phytoliths In Prehistoric Diet Of The Lower Pecos Region, Texas, Dennis Danielson, Karl Reinhard

Karl Reinhard Publications

Recent research demonstrates that silica phytoliths of dietary origin are associated with microwear of human teeth. Previous research has shown that severe enamel microwear and dental wear characterizes Archaic hunter-gatherers in the lower Pecos region of west Texas. Calcium oxalate crystals are especially common in Archaic coprolites. The vast majority are derived from prickly pear and agave, which were the dietary staples in west Texas for 6,000 years. The calcium oxalate phytoliths are harder than enamel. Therefore, calcium oxalate crystals are the most likely source of previously documented dental microwear and wear in the lower Pecos region.Am J Phys Anthropol …

Mummies And Mummification Practices In The Southern And Southwestern United States, Mahmoud Y. El-Najjar, Thomas M. J. Mulinski, Karl Reinhard

Karl Reinhard Publications

Mummification was not intentional for most North American prehistoric cultures. Natural mummification occurred in the dry areas of North America, where mummies have been recovered from rock shelters, caves, and overhangs. In these places, corpses desiccated and spontaneously mummified. In North America, mummies are recovered from four main regions: the southern and southwestern United States, the Aleutian Islands, and the Ozark Mountains of Arkansas. This chapter is limited to a discussion of burial practices and a tabulation of the location of mummies in the southwestern United States with some comments on mummies of the southern United States, including the Ozarks.