Immunology and Infectious Disease Commons^™

Improved Selective Medium For The Isolation Of Treponema Hyodysenteriae, Robert A. Kunkle, J. M. Kinyon

School of Veterinary and Biomedical Sciences: Faculty Publications

An agar medium with improved selection for Treponema hyodysenteriae was developed. Cultures of T. hyodysenteriae and T. innocens, feces from 11 clinically normal pigs, and colonic contents from 6 pigs with gross lesions consistent with swine dysentery were diluted in phosphate-buffered saline and plated on Trypticase soy agar (BBL Microbiology Systems, Cockeysville, Md.) with 5% citrated bovine blood (TSA), TSA with 400 ,ug of spectinomycin per ml (TSA-S400), TSA-S400 with 25 ,ug each of colistin and vancomycin per ml, and TSA with 5% pig feces extract and five antimicrobial agents (spiramycin, rifampin, vancomycin, colistin, and spectinomycin) (BJ). Viable numbers …

Formation Of Influenza Virus Particles Lacking Hemagglutinin On The Viral Envelope, Asit K. Pattnaik, Donald J. Brown, Debi P. Nayak

School of Veterinary and Biomedical Sciences: Faculty Publications

We investigated the intraceUular block in the transport of hemagglutinin (HA) and the role of HA in virus particle formation by using temperature-sensitive (Is) mutants (1s134 and 1s61S) of inOuenza virus AlWSN/33. We found that at the nonpermissive temperature (39.5°C), the exit of ts HA from the rough endoplasmic reticulum to the Golgi complex was blocked and that no additional block was apparent in either the exit from the Golgi complex or post-Golgi complex transport. When MDBK ceUs were infected with these mutant viruses, they produced noninfectious virus particles at 39.5°C. The efficiency of particle formation at 39.5°C was essentiaUy …

Effect Of Membrane Filtration Of Antimalarial Drug Solutions On In Vitro Activity Against Plasmodium Falciparum, J. Kevin Baird, C. Lambros

United States Public Health Resources

Antimalarial activities ofchloroquine, mefloquine, amodiaquine, and quinine in vitro against Plasmodium falciparum were diminished as a consequence ofmembranefiltration. Filtered drug solutions gave ID₅₀ values up to 25-fold greater than those of non-filtered (ethanol-sterilized) drug solutions. Loss ofactivity byfiltration was overcome by increasing the drug concentration prior to filtration. Water solutions filtered through Millex-GSfilter units consistently showed an absorbance maximum at 277 nm, accompanied by a lesser peak at 225 nm. Water filtrates from Nucleopore and Millex-G Vfilters showed no absorbance at 277 nm and only slight absorbance was evidentfor the Gelmanfilter unit. Activity losses were attributed to extractable contaminating …

Identification Of Four Complementary Rna Species In Akabane Virus-Infected Cells, Asit K. Pattnaik, G. Abraham

School of Veterinary and Biomedical Sciences: Faculty Publications

The analysis of RNA extracted from purified Akabane virus demonstrated the presence of three size classes of single-stranded RNAs with sedimentation coefficients of 31S (large, L), 26S (medium, M), and 13S (small, S). Molecular weights of these RNA species were estimated to be 2.15 x 10⁶,1.5 X 10⁶, and 0.48 x 10⁶ for the L, M, and S RNAs, respectively. Hybridization analysis involving viral genomic RNA and RNA from virus-infected cells resulted in the identification of four virus-specific cRNA species in infected cells. These cRNAs were found to be nonpolyadenylated by their inability to bind …

Some Aspects Of Carbohydrate Metabolism In The Cercariae Of Schistosoma Mansoni Sambon, 1907, A. Eugene Dunham Jr.

Loma Linda University Electronic Theses, Dissertations & Projects

Cercariac of Schistosoma mansoni were studied for glycogen utilization, uptake of glucose from solution, lactic acid production, and respiration.

Using the anthrone method of determination of carbohydrate, the amount of glycogen used by a single cercaria in 4 hours is determined as 43.2 x 10^-4 μg ± 6.6 x 10^-4 μg.

Glucose was not observed to have been used from solution under the conditions of the experiment, even in the presence of trehalose. The determinations were made by the Glucostat method.

After deproteinization and desugarization, aliquots of supernatant from cercarial suspensions were analyzed for lactic acid using p-phenylphenol …

The Effect Of Varying Protein Intake On Trichinella Spiralis Infection In Mice, Clare K. Kwan

Loma Linda University Electronic Theses, Dissertations & Projects

Three hundred Swiss Webster albino mice three weeks of age were placed on low (8%), medium (24%), high (48%) protein levels and a commercial laboratory animal feed, Purina Chow for various lengths of time: (1) simultaneously with infection, and four weeks thereafter, (2) three weeks before infection and throughout the four-week infection period, and (3) six weeks prior to infection and during the infection period. The protein source was casein. Sixty of the 300 mice used served as noninfected controls, and the other 240 were each infected with 400 washed, suspended Trichinella spiralis larvae obtained from a rat by the …

Viral Infections Of Domestic Animals, George A. Young

School of Veterinary and Biomedical Sciences: Faculty Publications

There is a strong tendency in current research on mammalian virus diseases to concentrate on virus activity at a cellular or subcellular level. This trend is summarized adequately by Enders in relation to viral replication as follows: ','The large and ever-increasing volume of published experimental work on viral replication strikingly reveals the central position of this phenomenon in contemporary virological research. There are good reasons, both biological and practical, underlying this intensive effort to understand, i n detail, how a virus particle, without energy transforming apparatus of its own, manages to utilize the metabolic equipment of the cell to produce …

Worm Eggs: Cost You Money, E.F. Frolik, E.W. Janike

School of Veterinary and Biomedical Sciences: Faculty Publications

Contents

One Million Eggs Daily.......... 3

Damaging Trip......... 4

Worm Remedies......... 5

Modern Worm Remedies......... 6

A Control Program.......... 8

The Corn-Stalk Disease In Cattle, Frank S. Billings

School of Veterinary and Biomedical Sciences: Faculty Publications

MORPHO-BIOLOGICAL CORRESPONDENCE BETWEEN TWO OR MORE MICRO- ETIOLOGICAL ORGANISMS NOT SUFFICIENT GROUNDS FOR PRONOUNCING THE DISEASES WITH WHICH THEY ARE CONNECTED IDENTICAL. The details of this discussion will be found in my report on the swine-plague. It is necessary, however, to touch upon the essential points here also. As was there shown, Hueppe asserts that the European diseases previously mentioned as being caused by a member of this group of belted, ovoid germs, viz., the “ Huhne Cholera, Kaninchen Septikasmie, und Wild Seuche ’ ’ are all one and the same disease, because their micro-etiological organisms have the same form, …

Keratitis Contagiosa In Cattle, Frank S. Billings

School of Veterinary and Biomedical Sciences: Faculty Publications

This is not a new disease by any means, so far as the United States are concerned, nevertheless I have been unable to find any description of it in the literature at my command. While new to myself until the past Summer, there have been quite a number of reports of its existence, and complaints about it, from farmers and breeders of cattle in some of the live-stock journals of our western States. Under these circumstances, it would seem that a description of its clinical phenomena and gross pathological lesions may not be without scientific interest to the opthalmologist, and …

Inoculation—Vaccination, Frank S. Billings

School of Veterinary and Biomedical Sciences: Faculty Publications

When one comes to discuss this question with the members of the profession as he casually meets them, he is often surprised to find how uncertain and clouded the prevailing ideas are upon the subject. In fact, the majority of physicians do not seem to have any clear conception of the act connected with the words “ inoculation ” and “ vaccination.”