Immunology and Infectious Disease Commons^™

Separate Metabolic Pathways Leading To Dna Fragmentation And Apoptotic Nuclear Chromatin Condensation, D. Sun, Shibo Jiang, Li-Mou Zheng, David M. Ojcius, John Ding-E. Young

All Dugoni School of Dentistry Faculty Articles

Apoptosis is the predominant form of cell death observed in a variety of physiological and pathological conditions such as cancer involution, insect metamorphosis, the development of the immune and nervous systems, and embryogenesis. The typical nuclear changes taking place in apoptotic cells include extensive condensation of chromatin and internucleosomal DNA fragmentation into units of 200 base pairs. However, the mechanisms responsible for both chromatin condensation and DNA fragmentation have yet to be elucidated. In this study, micrococcal nuclease and the divalent cations, Ca2+ and Mg2+, were applied to isolated nuclei in an attempt to reconstitute in vitro the digestion of …

Control Of Cheesy Gland In Sheep, Michael Paton

Journal of the Department of Agriculture, Western Australia, Series 4

Cheesy gland is a widespread problem in Western Australian sheep flocks. Less than 1 per cent of flocks are free of this disease, and all sheep in one line of 550 cull ewes slaughtered at Katanning Abattoir were infected.

A study of what affects new cheesy gland . infection found that shower dipping and keeping sheep under cover after shearing increased new infections. Farmers who shower dip sheep should consider vaccinating them against cheesy gland. Sheep should be let out into the open as soon as possible after shearing.

The total cost of the disease in Australia is about $30 …

Purification And Ligand Binding Of A Soluble Class I Mhc Molecule Consisting Of The First Three Domains Of H-2kd Fused To B2-Microglobulin Expressed In The Baculovirus/Insect Cell System, Francois Godeau, Immanuel F. Luescher, David M. Ojcius, Cecile Saucier, Estelle Mottez, Lucien Cabanie, Philippe Kourilsky

All Dugoni School of Dentistry Faculty Articles

A recombinant baculovirus encoding a single-chain murine major histocompatibility complex class I molecule in which the first three domains of H-2Kd are fused to beta 2-microglobulin (beta 2-m) via a 15-amino acid linker has been isolated and used to infect lepidopteran cells. A soluble, 391-amino acid single-chain H-2Kd (SC-Kd) molecule of 48 kDa was synthesized and glycosylated in insect cells and could be purified in the absence of detergents by affinity chromatography using the anti-H-2Kd monoclonal antibody SF1.1.1.1. We tested the ability of SC-Kd to bind antigenic peptides using a direct binding assay based on photoaffinity labeling. The photoreactive derivative …

Miscellaneous Publication 39/91 - How To Eradicate Virulent Footrot From Your Property. A Farmer's Guide., Dianne Hopkins

Animal production and livestock published reports

The first thing to understand about virulent footrot is that it can occur on any farm in the medium to high rainfall zone.

People often associate footrot with properties managed by inefficient farmers. This is a belief based on fallacy. Many good farmers have found the disease in their sheep, including high profile stud producers.

Footrot can strike anywhere and without warning.

The important thing to remember is, that once it is found, getting to know the disease and understanding it will help you beat it. This manual, the video and your stock inspector will help you eradicate footrot from …

Cytolytic And Ion Channel Forming Properties Of The N-Terminus Of Lymphocyte Perforin, David M. Ojcius, Pedro M. Persechini, Li-Mou Zheng, Paulo C. Notaroberto, Sandro C. Adeodato, John Ding-E. Young

All Dugoni School of Dentistry Faculty Articles

Perforin lyses cells by binding to the target cell membrane, where it polymerizes into large nonspecific pores. It is shown here that the first 34 amino acids of the N-terminal region of either human or murine perforin are soluble in aqueous medium and spontaneously insert into membranes. The N-terminal peptides lyse liposomes and nucleated cells, and they form ion channels in planar bilayers, some of which are comparable to those previously described for perforin. The lytic activity of the N-terminal domains does not require calcium, is independent of the lipid headgroup composition, and can be inhibited by heparin. Tumor cells …

Evidence For The Elevation Of Serum Carcinoembryonic Antigen And Tumor-Associated Glycoprotein-72 Levels In Patients Administered Interferons, John Greiner, Fiorella Guadagni, David Goldstein, Ernest Borden, Roy Ritts, Patricia Witt, Albert Lobuglio, Mansoor Saleh, Jeffrey Schlom

Haematology and Oncology, East Africa

Sera were collected from 111 patients diagnosed with adenocarcinoma or nonadenocarcinoma malignancies who received different schedules of interferon (IFN)-γ or IFN-βser alone or in combination. Serum carcinoembryonic antigen (CEA) and tumor-associated glycoprotein-72 (TAG-72) antigen levels were measured to determine whether interferon could enhance the tumor shedding and, thereby, the serum level of either tumor antigen. Less than 10% of the sera samples from patients diagnosed with nonadenocarcinoma malignancies (e.g., hairy cell leukemia, melanoma) had positive titers of TAG-72 or CEA, and interferon neither increased nor resulted in the appearance of either tumor antigen in those sera. In contrast, 59.2% and …

Pharmacokinetics, Immune Response, And Biodistribution Of Iodine-131-Labeled Chimeric Mouse/Human Igg1, K 17-1a Monoclonal Antibody, Ruby Meredith, Albert Lobuglio, Walter Plott, Roger Orr, Ivan Brezovich, Charles Russell, Elizabeth Harvey, Michael Yester, Mansoor Saleh

Haematology and Oncology, East Africa

Pharmacokinetics,immunogenicity,and biodistributionof a 131I-Iabeledmouse/human chimenc monoclonal antibody (C17-1A) was studiedin six metastaticcoloncancerpatients. Pharmacokinetics obtalned from serum radioactivity or chi mera concentration were identical after 5 mCi of 131I(>17-1A withmeanalphahalf-livesof 17.6±2.3 and19.7±2.9 and meanbeta half-livesof 100.9±16.1 and 106.4±14.1 hr, respectively. HPLC analysis documented the monomeric chi rneiic17-lA withoutevidenceof immunecomplexesorfree 1311 None of the patients developed antibody after ‘31I-chi merle17-1A exposure.Radiolocalizationoccurredin known areas of disease >4 cm in all patients. The half-life of total body radioactivity was 58 ±7 hr by whole-body counts and 64 ±13 hr by urine measurements. Whole-body and bone marrow dose estimates ranged from 0.75-1 .03 and …

Monocyte-Platelet Interaction In Immune And Nonimmune Thrombocytopenia, Mansoor Saleh, D L. Moore, J Y. Lee, A F. Lobuglio

Haematology and Oncology, East Africa

Platelets from 24 patients with immune thrombocytopenia resistant to standard therapy (refractory ITP), 35 patients with nonimmune thrombocytopenia (non-ITP), and 32 normal donors were studied in regard to platelet surface-bound IgG (PBIgG) and the ability of these platelets to be bound by human monocytes in vitro (monocyte-platelet rosette assay). Fourteen (58%) of the platelet samples from refractory ITP patients but none (0%) from the non-ITP or control donors had PBIgG greater than 800 molecules IgG/platelet. Seventeen of 24 (71%) of the ITP patients had platelets which demonstrated increased monocyte- platelet rosette formation [rosette index (RI) greater than 2], whereas only …

Current Issues And Future Directions In The Scientific Response To The Aids Epidemic, Anthony S. Fauci

DSLS 1988-1989

(Abstract taken from the Distinguished Scientist Lecture Series Program 1988-1989).

The recording is unavailable.

Dr. Fauci, Director of the National Institute of Allergy and Infectious Diseases (NIAID) of the National Institute of Health (NIH), also serves as the Associate Director of the NIH for research on the acquired immunodeficiency syndrome (AIDS) and as Director of the NIH Office of AIDS Research. A native of Brooklyn, New York, Dr. Fauci received his M.D. degree from Cornell University Medical College in 1966. After completing an internship at Cornell Medical Center in New York City, he began his work at the NIH as …

Clinical Use Of A Standard Kit-Preparation Of Radiolabeled Monoclonal Antibody 96.5 In The Diagnostic Imaging Of Metastatic Melanoma., Mansoor Saleh, D M. Miller, L Peterson, C D. Russell, M W. Unger

Haematology and Oncology, East Africa

We studied the efficiency of a standard-kit preparation using 1 mg 111In-labeled 96.5 monoclonal antibody in combination with 19 mg of unlabeled antibody in the diagnostic imaging of 27 patients with documented metastatic melanoma. Twenty-three of 26 patients (88%) demonstrated immunoscintigraphic localization of tumor. Of 104 metastatic sites previously documented by conventional studies, 62 (60%) were identified by immunoscintigraphy. A total of 77 sites demonstrated localization of radiolabeled antibody. Fifty-four (70%) corresponded to known sites of disease; eight sites (10%) were "discovered" by immunoscintigraphy and subsequently confirmed by conventional studies; 15 imaged sites (20%) could not be confirmed by conventional …

The Effect Of Sodium Chloride On Beta-Hemolytic Streptococci, Bola Fashola

Masters Theses & Specialist Projects

The drug of choice for the treatment of Stieptococcal pharyngitis is penicillin G. However, a common home remedy prescribes the use of salt-water solutions for gargling.

Members of Beta -hemolytic streptococcal groups A, B, and C were isolated from the upper -respiratory tracts of patients diagnosed as having streptococcal pharyngitis. These cultures we:e obtained from HCA Greenview Hospital (Bowling Green, Kentucky) and used to study the effects of sodium chloride on the isolates.

The minimum inhibitory concentration of sodium chloride was determined for each of eight hospital isolates. Croup A streptococci were inhibited at a concentration of 7.2% sodium chloride …

Footrot In Sheep And Goats, Department Of Agriculture, Western Australia

Animal production and livestock published reports

Reference to sheep in this pamphlet applies equally to goats.

Footrot is a contagious disease of sheep and goats caused by the bacterium Baueroides nodosus.

The bacterium can cause varying degrees of damage to the horn of the foot. The resulting infection can lead to lameness and serious loss of body condition.

Lectins, El-Nasir Lalani, M Wells

Department of Pathology and Laboratory Medicine

No abstract provided.

Myxomatosis In Western Australia, D R. King, S. H. Wheeler

Journal of the Department of Agriculture, Western Australia, Series 4

Since myxomatosis was introduced to Western Australia in the early 1950s rabbit plagues have ceased. However, myxomatosis has not proven to be the whole answer to the rabit problem.

Rabits continue to damage crops and pastures in many areas and resurgences of rabbit populations are reported from time to time.

Drs D.R. King and S. H. Wheeler of the Agricultural Protection Board's research section have been studying the way myxomatosis epidemics occur and spread in rabbit populations in a range of Western Australian environments. This research will provide data for planning effective control strategies intergrating the various control options available …

Effects Of Various Treatments On The Distribution In Rats Of Immune Complexes Containing Antigens Of Mulv Leukemia Virus, Wail M. Siag, Jean Matchett, Joe M. Jones

Journal of the Arkansas Academy of Science

Immune complexes (IC) containing antigens of MuLV (¹²⁵lp30-anti-p30 or ¹²⁵lp70-anti-gp70) and formed in vitro or in vivo were sequestered primarily in the spleen. Treatments of rats with trypan blue, known to inhibit the reticuloendothelial system (RES) and diethylstilbestrol (DES), known to stimulate the RES, resulted in slight but not significant reduction of uptake of IC by the spleen. Splenectomy did not increase sequestration of IC by the liver nor did it change the distribution of IC in other tissues. Protein A, a cell wall protein of Staphylococcus aureus, when injected with IC resulted in a 10-fold reduction of uptake of …

Electrophoretic And Immunological Studies On The Relationship Of The Brachyphyllinae And The Glossophaginae, Robert J. Baker, Rodney L. Honeycutt, Michael L. Arnold, Vincent M. Sarich, Hugh H. Genoways

University of Nebraska State Museum: Mammalogy Papers

Electrophoretic and albumin immunological data indicate that the Brachyphyllinae as currently conceived is a natural assemblage, with Erophylla sezekorni and Phyllonycteris aphylla being more closely related to each other than either is to Brachyphylla cavernarum. In both data sets, values that distinguish Erophylla from Phyllonycteris are in the general range of values that characterize congeneric species of mammals. Immunological distance values for the species Glossophaga soricina, Monophyllus redmani, Anoura caudifer, Leptonycteris sanborni, Choeroniscus minor, and Hylonycteris underuoodi indicate that these taxa are approximately equidistant from the Brachyphyllinae. Immunological comparisons of Glossophaga and Monophyllus to Anoura, Leptonycteris, Choeroniscus, …

The Biological Effect Of Evernimicin B On Bacillus Subtilis W2 3 And Three Antibiotic-Resistant Strains Of Staphylococcus Aureus, Steven Bogach

Masters Theses & Specialist Projects

One strain of Bacillus subtilis and three antibiotic-resistant strains of Staphylococcus aureus were employed to determine the biological effects of everninomicin B (EvB), a naturally occurring antibiotic produced by Micromonospora carbonacea (NRRL 2972) and M. carbonacea var. aurantiaca. Minimum inhibitory concentration (MIC) of EvB for B. subtilis was 1.2 x 10^-3 µmole/ml of EvB in glucose minimal broth and 2.6 x 10^-3 µmole/ml of EvB in nutrient broth. MIC values of EvB for S. aureus were 3.25 x 10^-4 µmole/ml of EvB for strains resistant to penicillin or tetracycline and 6.5 x 10^-4 µmole/ml of …

Ryegrass Toxicity Organism Found On Other Grasses, D L. Chatel, J. L. Wise, A. G. Marfleet

Journal of the Department of Agriculture, Western Australia, Series 4

Yellow slime disease caused by Corynebacterium sp. has recently been found in three grass species.

The diseased grasses were found in the field growing with each other and with affected toxic annual ryegrass.

Progress In Mastitis Control : A Simple Control Programme That Works, G R. Olney, R. K. Mitchell

Journal of the Department of Agriculture, Western Australia, Series 4

Intensive Department of Agriculture work on the mastitis problem has resulted in development of a simple control programme that works in W.A. dairy herds.

Farmern receive regular advice on their herd mastitis levels, and advice is available on overcoming specific problems.

Bibliography Of Diseases And Parasites Of Marine Fish And Shellfish (With Emphasis On Commercially Important Species), Carl J. Sindermann

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The literature on diseases and parasites of marine animals has been accumulating at an accelerating rate in recent decades, and at a seemingly geometrical rate in the past few years. Reviews of selected aspects of the subject have appeared (Cheng, 1967; Sindermann, 1966; Sindermann and Rosenfield, 1967). References listed in these papers include a significant, but still a small part, of the available literature. With the proliferation of journals in many languages throughout the world, a complete bibliography, even in a narrow area of research, is almost an impossibility. Then too, the daily appearance of new published information represents an …

A Control Programme For Cheesy Gland In Sheep, M R. Gardiner

Journal of the Department of Agriculture, Western Australia, Series 4

CASEOUS LYMPHADENITIS, or "cheesy gland" as it is usually known in Australia, is a common disease of sheep which is very important in the economics of the lamb and mutton industry.

Myxomatosis : Its Decline As A Killer, C D. Gooding

Journal of the Department of Agriculture, Western Australia, Series 4

ALTHOUGH nearly every farmer in Western Australia is familiar with myxomatosis, very few landowners really understand the factor causing the disease to spread or, just as importantly, why the severity of the disease is declining.

Ixodid Ticks: Possible Vectors Of Tuberculosis, Ya. A. Blagodarnyy, I. N. Blekhman, M. P. Yukunin

United States Navy: Publications

"From these tests, we established that artificially and naturally fed ticks are susceptible to the infective source and preserve tuberculosis mycobacteria in the body for a long period."

Enhanced Phagocytosis Of Salmonella Enteritidis With Hypoagglutinating Mouse Antisera, Jack Lockhart, Leo J. Paulissen

Journal of the Arkansas Academy of Science

No abstract provided.

Rapid Identification Of Streptococcal Infections Using Fluorescent Antibody Techniques, James H. Meadows

Biology ETDs

The use of fluorescent microscopy coupled with immunological techniques offer new methods for the demonstration of antigen-antibody reactions. The adaptation of these techniques to grouping of beta hemolytic streptococci makes feasible their further adaptation toward identification of group A streptococci in clinical material. Commercially available labeled antibodies against group A beta hemolytic streptococci were obtained for use in this study. A method for the determination of labeled antisera specificity is proposed and a technique for absorbing out any heterologous reacting antibody is described. Using this commercial labeled antisera, subsequent to specificity studies plus absorption if necessary, adaptations of basic fluorescent …

Ovine Vibriosis : A New Cause Of Sheep Infertility, M R. Gardiner, M. E. Nairn, I. J. Miller

Journal of the Department of Agriculture, Western Australia, Series 4

Vibrionic abortion of sheep has caused heavy losses in a number of wheatbelt flocks. In several flocks up to 50 per cent, of the ewes aborted as a result of this newly reported disease.

This article gives the history of the disease in this State, and describes the symptoms for the benefit of other farmers whose flocks may be affected. Ewes become immune after the first attack.

Antibiotics And Sterilisers In The Dairy Industry, K Needham, N. Ingleton

Journal of the Department of Agriculture, Western Australia, Series 4

A S a consequence of the rapid advances made in all aspects of technology there are an ever increasing number of preparations becoming available to the dairy farmer, to assist both in the control of disease in his crops, pastures and animals and to maintain and enhance the quality of his dairy products.

These are—antibiotics, sterilisers, detergents, various weedicides and pesticides

Water: Its Inluence On Health And Disease, John Wilby

Student and Lippitt Prize Essays

An insight into the health effects of water and diseases it may carry; provides alternative ideas and solutions for sanitizing.