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Molecular Genetics Commons™

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1995

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Articles 1 - 12 of 12

Full-Text Articles in Molecular Genetics

Active Inhibition Of T Cell Proliferation By Monocytes, Dean Anthony Lee Dec 1995

Active Inhibition Of T Cell Proliferation By Monocytes, Dean Anthony Lee

Loma Linda University Electronic Theses, Dissertations & Projects

Monoclonal antibodies (mAb) to MHC class II molecules (anti-class II mAb) have been well described in their ability to inhibit T cell activation. The most current model is that anti-class II mAb interfere directly with the T cell receptor-major histocompatibility complex crosslinking which is necessary for T cell activation. The anti-CD3 mAb, OKT3, is an MHC class II molecule-independent mitogen which causes T cell activation by direct ligation of the TCR-CD3 complex. OKT3 and a panel of anti-class II mAb were used to dissect the individual roles played by these two receptors in activation and inhibition of peripheral blood mononuclear …

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Involvement Of P59FynT In Interleukin-5 Receptor Signaling, Mark W. Appleby, James D. Kerner, Sylvia Chien, Charles R. Maliszewski, Subbarao Bondada, Roger M. Perlmutter Sep 1995

Involvement Of P59FynT In Interleukin-5 Receptor Signaling, Mark W. Appleby, James D. Kerner, Sylvia Chien, Charles R. Maliszewski, Subbarao Bondada, Roger M. Perlmutter

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Previous studies implicate the nonreceptor protein tyrosine kinase (PTK) p59fyn in the propagation of signals from the B cell antigen receptor. To elucidate the functions of this kinase, we examined B cell responsiveness in mice engineered to lack the hematopoietic isoform of p59fyn. Remarkably, antigen receptor signaling was only modestly defective in fynTnull B cells. In contrast, signaling from the interleukin (IL)-5 receptor which ordinarily provides a comitogenic stimulus with antiimmunoglobulin, was completely blocked. Our results document the importance of p59fynT in IL-5 responses in B cells, and they support a general model …

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Developmental Regulation Of The Murine Cd4 Gene, Rhonda Kay Hansen Aug 1995

Developmental Regulation Of The Murine Cd4 Gene, Rhonda Kay Hansen

Loma Linda University Electronic Theses, Dissertations & Projects

CD4 is a membrane bound glycoprotein that plays an important role in T cell development and activation. An extensive analysis of the CD4 locus identified potential transcriptional control elements located 5' of the promoter and within the first intron. Since the molecular mechanism for the control of CD4 expression during T cell development had not been determined, it is the focus of this dissertation. A series of recombinant DNA constructs were made to determine the contribution of putative transcriptional control elements in regard to CD4 expression, and to identify elements being used to control CD4 expression during T cell development. …

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Regulation Of The Insulin-Like Growth Factor System By Retinoic Acid In Human Osteoblast Cells, Yuehua Zhou Jun 1995

Regulation Of The Insulin-Like Growth Factor System By Retinoic Acid In Human Osteoblast Cells, Yuehua Zhou

Loma Linda University Electronic Theses, Dissertations & Projects

Retinoic acid (RA) is an important regulator of growth and differentiation in many cell types, including bone. However, its effects on human osteoblast cell growth and differentiation have not been well studied. Therefore, I investigated the effect of RA on proliferation and differentiation of normal human bone cells (HBCs) and human osteosarcoma, SaOS-2 cells. RA decreased baseline as well as serum-stimulated proliferation in normal HBCs. To determine the effect of RA on differentiation, expression of several osteoblastic differentiation markers were studied. RA decreased type I procollagen mRNA levels and l,25(OH)2D3-stimulated osteocalcin and alkaline phosphatase (ALP) mRNA …

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A Model Of Cytomegalovirus Association With The Development Of Heart Graft Atherosclerosis, Evan Skowronski Jun 1995

A Model Of Cytomegalovirus Association With The Development Of Heart Graft Atherosclerosis, Evan Skowronski

Loma Linda University Electronic Theses, Dissertations & Projects

The development of graft atherosclerosis in heart transplant recipients has been associated with the development of post-transplant cytomegalovirus infection. The mechanism of this involvement, however, remains unclear. I undertook a study to determine the role of cytomegalovirus in the development of graft atherosclerosis. First, a rapid method of detecting cytomegalovirus based on capillary polymerase chain reaction and gel electrophoresis was employed to determine the presence of cytomegalovirus in a wide variety of clinical samples. Detection of cytomegalovirus is a lengthy procedure under normal conditions, and timely detection of cytomegalovirus in transplant recipients may allow intervention early enough to block the …

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Characterization Of A Saccharomyces Cerevisiae Strain Deleted For The Rad27 Gene; A Structural Homolog Of The Rad2 Nucleotide Excision Repair Gene, Michael S. Reagan, Wolfram Siede, Errol C. Friedberg Mar 1995

Characterization Of A Saccharomyces Cerevisiae Strain Deleted For The Rad27 Gene; A Structural Homolog Of The Rad2 Nucleotide Excision Repair Gene, Michael S. Reagan, Wolfram Siede, Errol C. Friedberg

Biology Faculty Publications

No abstract provided.

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Enhancer Trap Technique: A Novel Tool For Identification And Developmental Characterization Of Genes Of Drosophila, Amit Singh Mar 1995

Enhancer Trap Technique: A Novel Tool For Identification And Developmental Characterization Of Genes Of Drosophila, Amit Singh

Biology Faculty Publications

The classical technique of mutational screen for identification of genes controlling early development has now approached saturation. A new era in genetic identification and developmental characterization of genes in Drosophila has commenced with the advent of the enhancer trap technique. This technique involves mobilization of a P-lacZ vector to diverse chromosomal locations in the fruit fly genome to bring it under the regulation of developmentally expressed genes or their enhancer elements. The technique offers a strikingly elegant method of gaining entry into fruit fly genes.

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Isolation Of A Mouse Cdna Encoding Mtj1, A New Murine Member Of The Dnaj Family Of Proteins, Shannon E. Brightman, Gregory L. Blatch, Bruce R. Zetter Feb 1995

Isolation Of A Mouse Cdna Encoding Mtj1, A New Murine Member Of The Dnaj Family Of Proteins, Shannon E. Brightman, Gregory L. Blatch, Bruce R. Zetter

Biology Faculty Publications

We report the isolation and sequencing of MTJ1, a 1792-bp cDNA from an M27 murine lung carcinoma cell line. The largest ORF within MTJ1 encodes a 63,869-Da protein, containing a 73-amino-acid (aa) sequence (the J domain) that is conserved in proteins of the DnaJ family of chaperonins. The J domain of MTJ1 is bracketed by potential transmembrane domains in a similar configuration to the J domain of the yeast DnaJ-like protein, SEC63. Polyclonal antibodies raised against deduced aa sequences within MTJ1 recognized antigens of 62, 42 and 41 kDa that were enriched in the nuclear and heavy microsome subcellular fractions …

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An Improved Method For Chemical Devitellinization Of X-Gal Stained Drosophila Embryos, Amit Singh, Madhuri Kango-Singh, P. Sinha Feb 1995

An Improved Method For Chemical Devitellinization Of X-Gal Stained Drosophila Embryos, Amit Singh, Madhuri Kango-Singh, P. Sinha

Biology Faculty Publications

In Drosophila developmental biological studies, X-gal staining is commonly employed to study the spatio-temporal expression of the lacZ reporter gene in the transformed flies or their embryos. Study of the lacZ pattern in embryos often suffers from the lack of an efficient and high yieldirrg technique for devitellinization of X-gal stained embryos. Devitellinization techniques employed during antibody staining, in situ hybridization or embryonic cuticular preparations generally do not give satisfactory results when used for similar purpose in X-gal stained embryos. This results in the flaky appearance of the blue stain. We present here an improved chemical devitellinization technique which gives …

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Biotechnology : Exposing The Myths & Realities, Sue Sutherland, Alan Lymbery Jan 1995

Biotechnology : Exposing The Myths & Realities, Sue Sutherland, Alan Lymbery

Journal of the Department of Agriculture, Western Australia, Series 4

Biotechnology has become one of the buzz words of the 1990s. Sounds impressive but what's it all about? Sue Sutherland and Alan Lymbery unravel some of the jargon and explore its potential for Western Australian agriculture.

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Characterization Of A Mutant Strain Of Saccharomyces Cerevisiae With A Deletion Of The Rad27 Gene, A Structural Homolog Of The Rad2 Nucleotide Excision Repair Gene, Michael S. Reagan, Christopher Pittenger, Wolfram Siede, Errol C. Friedberg Jan 1995

Characterization Of A Mutant Strain Of Saccharomyces Cerevisiae With A Deletion Of The Rad27 Gene, A Structural Homolog Of The Rad2 Nucleotide Excision Repair Gene, Michael S. Reagan, Christopher Pittenger, Wolfram Siede, Errol C. Friedberg

Biology Faculty Publications

We have constructed a strain of Saccharomyces cerevisiae with a deletion of the YKL510 open reading frame, which was initially identified in chromosome XI as a homolog of the RAD2 nucleotide excision repair gene (A. Jacquier, P. Legrain, and B. Dujon, Yeast 8:121–132, 1992). The mutant strain exhibits increased sensitivity to UV light and to the alkylating agent methylmethane sulfonate but not to ionizing radiation. We have renamed the YKL510 open reading frame the RAD27 gene, in keeping with the accepted nomenclature for radiationsensitive yeast mutants. Epistasis analysis indicates that the gene is in the RAD6 group of genes, which …

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Molecular Cloning And Rare Cleavage Mapping Of Human 2p, 6q, 8q, 12q, And 18q Telomeres, Roberto A. Macina, Ken Morii, Xue-Lan Hu, Dimitri G. Negorev, Chrysanthe Spais, Lisa A. Ruthig, Harold C. Riethman Jan 1995

Molecular Cloning And Rare Cleavage Mapping Of Human 2p, 6q, 8q, 12q, And 18q Telomeres, Roberto A. Macina, Ken Morii, Xue-Lan Hu, Dimitri G. Negorev, Chrysanthe Spais, Lisa A. Ruthig, Harold C. Riethman

School of Medical Diagnostics & Translational Sciences Publications

Large terminal fragments of human chromosomes 2p, 6q, 8q, 12q, and 18q were cloned using yeast artificial chromosomes (YACs). RecA-assisted restriction endonuclease (RARE) cleavage analysis of genomic DNA samples from 11 unrelated individuals using YAC-derived probes confirmed the telomeric localizations of the half-YACs studied. The cloned Fragments provide telomeric closure of maps for the respective chromosome arms and will supply the reagents needed for analyzing and sequencing these distal subtelomeric regions.

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