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- Ananas comosus var. MD2 (1)
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- ArgR Protein; Xer Site Specific Recombination; Protein Fusion Study; Structure Function Relationship; Escherichia Coli Genetics (1)
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- Campylobacter jejuni (1)
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Articles 1 - 16 of 16
Full-Text Articles in Molecular Genetics
Environmental Dna (Edna) Assessment Of Fish And Decapod Diversity And Their Association With Cetacean Distribution In Matang Coastal Waters, Perak, Lim Chuan Hui
Student Works (2020-2029)
The estuarine and coastal regions of Matang, Perak, Peninsular Malaysia, are vital fishing grounds and recognised biodiversity hotspots, but are increasingly exposed to human activities. Understanding the biodiversity of marine fish and decapods is crucial for effective conservation efforts. Conventional biodiversity monitoring methods face several challenges, including invasiveness, inconsistencies in sampling techniques, reduced taxonomic accuracy, time-consuming processes, high costs, and often only providing approximate estimates. In this study, we evaluated the use of environmental DNA (eDNA) metabarcoding to assess marine fish and decapod diversity in Matang waters and to explore possible associations between fish assemblages and local cetacean occurrence. Water …
Differentiation Propensity, Safety, And Therapeutic Potential Of Mesenchymal Stem Cells Of Dental Origin, Musa Sabri
Differentiation Propensity, Safety, And Therapeutic Potential Of Mesenchymal Stem Cells Of Dental Origin, Musa Sabri
Student Works (2020-2029)
Introduction: A new area in medical research has emerged with the discovery of mesenchymal stem cells (MSCs) and their ability to differentiate into other cell types. Specifically, these cells are found in various tissues and have been utilized in the field of regenerative medicine to support tissue repair as well as in cell replacement. Lately, MSCs of dental origin have been the main players used for regenerative purposes although subtle differences based on their tissue source separate them. Objectives: This study aims to determine the differential propensity, safety, and potential therapeutic applications of dental-derived MSCs. This involves examining various aspects …
Molecular Characterisation Of Hsp70 Gene Expression, Histopathology And Immune System Of Snakehead Fish, Channa Striata, Under Temperature And Bacterial Stress, Eid Elhussein Ihlam Ibrahim
Molecular Characterisation Of Hsp70 Gene Expression, Histopathology And Immune System Of Snakehead Fish, Channa Striata, Under Temperature And Bacterial Stress, Eid Elhussein Ihlam Ibrahim
Student Works (2020-2029)
Snakeheads are air-breathing freshwater fishes containing two genera, Channa (Asia) and Parachanna (Africa). The Asian genus is dominated by Channa striata which is found in southern and Southeastern parts of the continent. In this research, environmental and bacteria stressor to Channa striata were investigated. HSP70 gene was studied as an environmental stress gene (ChHSP70). Results showed a cDNA sequence of the gene to be (~918bp). It was also shown the gene is highly similar to that of other fish species. Constructing a phylogenetic tree of the gene across vertebrate species revealed six main clusters; same class remained conserved by showing …
Genetic Variation Of Clonal Ananas Comosus Var. Md2 Plants After Mutagenic Treatment At Different Post-Recovery Periods, Mohd Raffi Anis Norsyahira
Genetic Variation Of Clonal Ananas Comosus Var. Md2 Plants After Mutagenic Treatment At Different Post-Recovery Periods, Mohd Raffi Anis Norsyahira
Student Works (2020-2029)
Studies on genetic variation in plants had become extensively employed in research for plant improvement and conservation. Due to their sessile nature, plants are exposed to various environmental stressors such as exposure to enhanced levels of ultraviolet (UV), ionizing and non-ionizing radiations. Exposure to these harmful radiations will result in various damages, ranging from DNA and chromosomal aberrations to phenotypic abnormalities. However, plants have evolved efficient DNA repair mechanisms to detect and repair any DNA damages caused by the exposure to these harmful stressors, therefore ensuring their survival. In this study, the effect of gamma radiation (as a source of …
Development Of Protocols For Tissue Culture And Mutagenesis In Two Variegated Sansevieria Sp., Irmawati Akma Abdul Hapiz
Development Of Protocols For Tissue Culture And Mutagenesis In Two Variegated Sansevieria Sp., Irmawati Akma Abdul Hapiz
Student Works (2010-2019)
Studies were conducted to develop the tissue culture protocol and mutagenesis through Gamma ray irradiation for two variegated Sansevieria species known as Sansevieria trifasciata var. Laurentii (goldband Sansevieria) and Sansevieria trifasciata var. Hahnii (birdnest Sansevieria). Seven combinations of BAP and NAA were chosen which are represented by letter A= 1.00 mg/I BAP, B = 1.00 mg/I BAP + 0.25 mg/I NAA, C = 2.00 mg/I BAP + 0.50 mg/I NAA, D = 2.00 mg/I BAP + 1.00 mg/I NAA, E = 3.00 mg/I BAP + 1.00 mg/I NAA, F = 4.00 mg/I BAP + 1.00 mg/I NAA and G = …
Molecular Characterization And Expression Analysis Of Putative Rpk1 And Putative Clv1 In Malaysian Indica Rice (Oryza Sativa L.) Cv.Mr219 And Malaysian Wild Rice (Oryza Rufipogon) Irgc105491., Law Yee Song
Student Works (2010-2019)
Two candidate yield-related genes, putative Receptor-like Protein Kinase 1 (putative RPK1) and putative CLAVATA1 Receptor Kinase (putative CLV1), were selected for molecular characterization and expression analysis. This was carried out using in four rice lines comprising the parents and two BC2F7 lines from a local rice breeding program, with Malaysian rice variety, Oryza sativa ssp. indica cv. MR219 as recurrent parent, and Oryza rufipogon (IRGC105491) as donor parent. Approximately 2.8 kb and 2.6 kb putative RPK1 cDNA nucleotide sequences were obtained from Oryza rufipogon and Oryza sativa ssp. indica cv. MR219 respectively. However, only 402 …
Characterization Of Microrna172 And Its Potential Target In Oil Palm (Elaeis Guineensis Jaq.), Fatemeh Mehrpooyan
Characterization Of Microrna172 And Its Potential Target In Oil Palm (Elaeis Guineensis Jaq.), Fatemeh Mehrpooyan
Student Works (2010-2019)
Oil palm (Elaeis guineensis) is the world’s major perennial monocot crop and contributes highly to the economies of South East Asia. As the oil palm fruit is the source of the main economic output for this crop, floral development is a critical process related to oil palm productivity. miR172 has been shown to be an important regulator of the AP2 family transcription factors involved in floral timing in a number of plants and belongs to a group of highly conserved miRNA that can be identified based on sequence homology. Six oil palm miR172 paralogs, representing two distinct mature miR172 sequences, …
Phylogeographical Patterns Of Neobalanocarpus Heimii (Dipterocarpaceae) For The Evolutionary History And Chain Of Custody Certification., Tnah Lee Hong
Student Works (2010-2019)
Tectonic movement and climatic oscillations during the Cenozoic have had dramatic effect on the biota of the tropical rain forest. This study aims to reveal the phylogeography and evolutionary history of a Peninsular Malaysian endemic tropical timber, Neobalanocarpus heimii (Dipterocarpaceae), based on chloroplast DNA (cpDNA) variation. Fifteen haplotypes were identified from 10 intraspecific variable sites of five non-coding cpDNA regions: trnL intron, trnS-trnG spacer, trnG intron, trnK intron and psbK-trnS spacer. Two major genealogical cpDNA lineages of N. heimii were elucidated: a widespread southern and a northern region. The species is predicted to survive in multiple refugia during climatic oscillation: …
Enterobacterial Repetitive Intergenic Consensus-Pcr (Eric-Pcr) Analysis Among Raw Vegetables Isolates Of Campylobacter Jejuni., Tuan Suraya Tuan Soh
Enterobacterial Repetitive Intergenic Consensus-Pcr (Eric-Pcr) Analysis Among Raw Vegetables Isolates Of Campylobacter Jejuni., Tuan Suraya Tuan Soh
Student Works (2010-2019)
A total of 20 (n=20) raw vegetable isolates of Campylobacter jejuni were examined for the presence of virulence genes and genetic diversity using enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) analysis. All C. jejuni isolates were obtained from the laboratory of Food Science and Biotechnology, University Putra Malaysia, Serdang, Selangor. When specific PCR amplification were determined, all raw vegetables isolates of Campylobacter jejuni contained cadF and ceuE genes. Whereas, 12 (12/20) isolates were positive cdtB and 6 (6/20) were positive cdtC. None of the C. jejuni isolates was cdtA positive. Using ERIC-PCR analysis, all 20 isolates of C. jejuni were …
Biolistic Transformation Of Oil Palm Using The Phosphomannose Isomerase (Pmi) Gene As The Positive Selectable Marker., Bahariah Bohari
Biolistic Transformation Of Oil Palm Using The Phosphomannose Isomerase (Pmi) Gene As The Positive Selectable Marker., Bahariah Bohari
Student Works (2010-2019)
The selectable marker system based on the Escherichia coli phosphomannose isomerase (pmi) gene was adapted for genetic transformation of oil palm. This system makes use of the pmi gene that encodes phosphomannose isomerase, which converts mannose-6-phosphate to fructose-6-phosphate and uses mannose as the selection agent. This is to anticipate the future requirement of using non-antibiotic resistance genes for the commercialization of transgenic oil palm. The use of antibiotic or herbicide-based selection systems has caused much public concern due to inadequate knowledge of the agents’ impact on the environment and on human health. In this study, four transformation vectors, namely pMI3, …
A Systematic Study Of Fagraea Sensu Lato And A Revision Of The Peninsular Malaysian Taxa., Sugumaran Manickam
A Systematic Study Of Fagraea Sensu Lato And A Revision Of The Peninsular Malaysian Taxa., Sugumaran Manickam
Student Works (2010-2019)
systematic study of the Peninsular Malaysian species of Fagraea sensu lato was undertaken. This was done in the light of recent revisionary work done for Borneo that documented 20 new species and demonstrated that previous species concepts for Fagraea were too broad. Parallel to this the distinction of the subgeneric groups recognised as sections, i.e., Cyrtophyllum, Fagraea and Racemosae, were investigated with molecular phylogenetic methods. Representative taxa from the Malay Peninsula and Borneo augmented by sequences from other taxa in the same subtribe and tribe were used in the molecular analysis. Gene sequences from ITS, trnL–F (trnL intron + trnL–F …
Computational Identification Of Micro Rna In Musa Sp Est Sequences., Tan Yew Seong
Computational Identification Of Micro Rna In Musa Sp Est Sequences., Tan Yew Seong
Student Works (2010-2019)
MicroRNAs (miRNAs) are small single-stranded non-protein-coding RNAs of about 22 nucleotides that play an important role in post-transcriptional gene regulation in animals and plants by targeting mRNAs for direct cleavage of mRNAs or repression of mRNA translation. In this study, a homology search based approach has been used for identifying the novel miRNA genes of Musa sp. using the BLAST program with a total of 42,978 Musa sp. expressed sequence tags (EST) were compared to a total of 959 previously known plant miRNA sequences. Forty-seven candidate miRNA with homology to previously known miRNAs were identified. By using the mFold3.1 program, …
Isolation And Development Of Microsatellite Markers Of Channa Striata., Faiza Omar
Isolation And Development Of Microsatellite Markers Of Channa Striata., Faiza Omar
Student Works (2010-2019)
Channa striata, locally known as haruan has long been regarded as a valuable edible fish and it is also consumed for its putative effects on wound healing. The main objective of the project was to develop microsatellite markers for use in the study of genetic diversity of the wild Channa striata populations in Malaysia. In this study, seven new microsatellite loci were isolated from Channa striata using a Random Amplified Microsatellite (RAMs) based technique which was an efficient and reliable method. Ten primers were used in population genetic studies of Channa striata.Sixty sets of microsatellite primer pairs were used in …
Molecular Identification Of Arowana Species Based On Cytochrome B And Cytochrome C Oxidase I (Coi) / Maryam Zahedi Fard, Maryam Zahedi Fard
Molecular Identification Of Arowana Species Based On Cytochrome B And Cytochrome C Oxidase I (Coi) / Maryam Zahedi Fard, Maryam Zahedi Fard
Student Works (2010-2019)
Mitochondrial DNA (mtDNA) markers has been widely used to better resolve the taxonomic identification as well as display the genetic relatedness of different species and closely related fish. The highly cost ornamental fish, Asian arowana (Scleropages formosus) of family Osteoglossidae has different colored strains that are geographically distributed in different regions around Southeast Asia. The taxonomic classification of these different strains still remains unclear and disputable. The partial sequences of the cytochrome b and the DNA barcoding gene, cytochrome C oxidase were analyzed and assessed for molecular identification; to differentiate the colored varieties of this species. The results of this …
Construction Of Escherichia Coli Arginine Repressor Fusion Protein And Analysis Of Its Function In Xer Site-Specific Recombination, Kholis Abdulrachim
Construction Of Escherichia Coli Arginine Repressor Fusion Protein And Analysis Of Its Function In Xer Site-Specific Recombination, Kholis Abdulrachim
Student Works (2000-2009)
In addition to its role in L-arginine biosynthesis in Escherichia coli, arginine repressor (ArgR), the product of the argR gene, also plays an essential role as an obligate accessory protein in Xer site-specific recombination system. A structure-function relationship study of ArgR was performed to understand more about its role in Xcr site-specific recombination. Fusion proteins between ArgRWT (wild-type ArgR) and a biotinylated peptide as well as between ArgRNV (a mutant ArgR) and a biotinylated peptide were constructed. The biotinylated peptide was fused in frame to the amino-tenninus of ArgR WT and ArgRNV, respectively. Xer recombination assays showed that the ArgRWT-biotinylated …
Application Of Ai In Medicine, Tarmizi Amirul Hisyam
Application Of Ai In Medicine, Tarmizi Amirul Hisyam
Student Works (2000-2009)
From the beginning of human civilization, mankind have been trying to fins solution on how to make life easier. They create, modify and adapt, as they would please their need. From the smallest things in our life to the biggest part of all technologies, has improved our life tremendously. Computer technology nowadays has an important role in education, communication, medicine, and many more. In the field of medicine, we have seen many support systems had been developed and use to help human in their daily lives. Application of AI in medicine was proposed in this reason in mind, as a …