Genetics and Genomics Commons^™

Cooperative Binding Of Heat Shock Factor To The Yeast Hsp82 Promoter In Vivo And In Vitro, Alexander M. Erkine, Serena F. Magrogan, Edward A. Sekinger, David S. Gross

Scholarship and Professional Work – COPHS

revious work has shown that heat shock factor (HSF) plays a central role in remodeling the chromatin structure of the yeastHSP82 promoter via constitutive interactions with its high-affinity binding site, heat shock element 1 (HSE1). The HSF-HSE1 interaction is also critical for stimulating both basal (noninduced) and induced transcription. By contrast, the function of the adjacent, inducibly occupied HSE2 and -3 is unknown. In this study, we examined the consequences of mutations in HSE1, HSE2, and HSE3 on HSF binding and transactivation. We provide evidence that in vivo, HSF binds to these three sites cooperatively. This cooperativity is seen …

Genetic Relationships Between Visual And Objective Measures Of Carcass Composition In Crossbred Lambs, H. E. Jones, G. Simm, W. S. Dingwall, R. M. Lewis

Department of Animal Science: Faculty Publications

The aim of this study was to estimate genetic and phenotypic (coroariances between objective measures and carcass visual scores, as a test of the potential value afvisual scores in selection programmes to improve carcass composition in crossbred lambs. In each of 1986, 1987 and 1988, 22 Suffolk rams were chosen with either high or low scores on an index designed to increase lean growth rate. These rams were joined with 18 to20 crossbred ewes each and theirlambs were grov.J11 on grass to one of three target live weights (35·5, 41·5 and 47·0 kg) for slaughter. The carcasses of 1881 lambs …

Developing A Gene Specific Probe For Phosphoenolpyruvate Carboxykinase Mrna, Stephen M. Kuntz

Seton Hall University Dissertations and Theses (ETDs)

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Ecdysteroid Titers In Mated And Unmated Drosophila Melanogaster Females, Lawrence G. Harshman, Anne M. Loeb, Beth A. Johnson

Lawrence G. Harshman Publications

Radioimmunoassay was used to determine ecdysteroid titers in mated or unmated Drosophila melanogaster females. Whole-body ecdysteroid titers increase after mating and this response is more pronounced after 12–24 hours than it is immediately after mating. In one experiment, females were mated to transgenic males deficient in accessory gland proteins to test whether these peptides mediate the observed increase in female whole-body ecdysteroid titers. Females mated to such transgenic males do not show a pronounced increase in whole-body ecdysteroid titers. The effect of mating on female hemolymph ecdysteroid titers was also investigated. Hemolymph ecdysteroid titers decrease after mating. The ecdysteroid titer …

Transcriptional Regulation Of The Bmp2 Gene: Retinoic Acid Induction In F9 Embryonal Carcinoma Cells And Saccharomyces Cerevisiae, Loree C. Heller, Yong Li, Kevin L. Abrams, Melissa B. Rogers

Bioelectrics Publications

Bmp2, a highly conserved member of the transforming growth factor-beta gene family, is crucial for normal development. Retinoic acid, combined with cAMP analogs, sharply induces the Bmp2 mRNA during the differentiation of F9 embryonal carcinoma cells into parietal endoderm. Retinoic acid (RA) also induces the Bmp2 gene in chick limb buds. Since normal Bmp2 expression may require an endogenous retinoid signal and aberrant Bmp2 expression may cause some aspects of RA-induced teratogenesis, we studied the mechanism underlying the induction of Bmp2. Measurements of the Bmp2 mRNA half-life and nuclear run-on assays …

Can Large Dsdna-Containing Viruses Provide Information About The Minimal Genome Size Required To Support Life?, James L. Van Etten

James Van Etten Publications

The genomes of a few viruses, such as Bacillus megaterium phage G (670 kb) and the chlorella viruses (330 to 380 kb), are larger than the predicted minimal genome size required to support life (ca. 320 kb). A comparison of the 256 proteins predicted to be required for life with the putative 376 proteins encoded by chlorella virus PBCV-1, as well as those encoded by other large viruses, indicates that viruses lack many of these “essential” genes. Consequently, it is unlikely that viruses will aid in determining the minimal number and types of genes required for life. However, viruses may …

The Expression Of The Crf1 Gene In Mouse Embryonic Development, Margaret Gruber

Presidential Scholars Theses (1990 – 2006)

A mouse gene cloned in the laboratory of James and Olivia Smith at Baylor College of Medicine was found to be involved in motor function. This gene was named CRFl for Clq-Related Factor 1, and using in situ hybridization, it was found to be expressed mainly in the brainstem of adult mice. There is a gene similar to the CRFl gene in humans. By determining this gene's role in mouse development, more will be known about the development of human motor function.

To determine the stage of embryonic development at which the expression of this gene …

Social Implications Of Genetic Testing, Karen H. Rothenberg

Faculty Scholarship

This paper examines the social implications of predictive genetic testing and its impact on the insurance industry. Although the Human Genome Project has the potential to improve the health of our nation, it also may serve as a means of highlighting genetic differences among individuals and ethnic groups. Thus, if we are to reach the full promise of the Project, society must address the public's fears of genetic discrimination in insurance and employment context. Following an analysis of state and federal legislation on genetic privacy and discrimination, the paper concludes with a challenge to the insurance industry to work with …

Nuclear Receptor Co-Repressor Gene Localizes To 17p11.2, A Frequently Deleted Band In Malignant Disorders, Michael W. Stacey, Jianxiang Wang, Rebecca L. Byrd, Johnson M. Liu, William G. Kearns

Bioelectrics Publications

The t(8;21) between the AML1 and ETO genes is a commonly seen genetic alteration in acute myeloid leukemia. Recently, we reported that the fusion partner ETO binds to the human nuclear receptor co-repressor (NCOR), a member of the NCOR/SIN3/histone deacetylase complex. This complex mediates transcriptional repression as a result of chromatin remodeling. Here, we used a combination of fluorescence in situ hybridization and hybrid panels to localize the human NCOR gene (NCOR) to chromosome band 17p11.2. The position of human NCOR on 17p11 raises the possibility of deranged transcriptional regulation in malignant disorders associated with deletions of 17p.

The Human Genome Project's Implications For Autonomy, Respect, And Professionalism In Medical Genetics, Roger B. Dworkin

Articles by Maurer Faculty

No abstract provided.

Plant Hybrid Zones And Insect Host Range Expansion, Diana Pilson

Diana Pilson Publications

The hybrid bridge hypothesis suggests that plant hybrids ‘‘bridge’’ the genetic gap between actual and potential host species, and that, for this reason, herbivorous insects are more likely to evolve an expanded host range in the presence of hybrids. While intuitively appealing, the hypothesis has two implicit assumptions: that phenotypic gaps between potential hosts limit host range, and that characters controlling host use are additively inherited in plant hybrids. Evaluation of these assumptions suggests that operation of the hybrid bridge hypothesis is relatively uncommon. In addition, the hypothesis has not been well integrated into existing theoretical and empirical work on …

Cynopterus Sphinx, Jay F. Storz, Thomas H. Kunz

Jay F. Storz Publications

Order Chiroptera, Family Pteropodidae, Subfamily Pteropodinae, Tribe Cynopterini, Subtribe Cynopterina, Genus Cynopterus. Five species are recognized: C. brachyotis, C. horsfieldi, C. nusatenggara, C. sphinx, and C. titthaecheileus (Koopman, 1993). A key to the species is given in Lekagul and McNeely (1977).

Potential Associations Among Genetic Markers In The Serotonergic System And The Antisocial Alcoholism Subtype, E. M. Hill, S. F. Stoltenberg, M. Burmeister, M. Closser, R. A. Zucker

Department of Psychology: Faculty Publications

Alcoholism is transmitted in families. The complexity and heterogeneity of this disorder has made it difficult to identify specific genetic correlates. One design with the potential to do so is the family-based association study, in which the frequencies of genetic polymorphisms are compared between affected and nonaffected members. Reduced central serotonin neurotransmission is associated with features of an antisocial subtype of alcoholism, although a primary deficit has not been traced to a particular component. Genetic markers related to the serotonergic system have been identified, located, and cloned. If associations can be discovered, the development process for pharmacotherapy could be facilitated. …

The Biotech Century (Book Review), Philip A. Reed

STEMPS Faculty Publications

No abstract provided.

Isozyme Polymorphism In Some Yellow-And Blue-Flowered Vigna Species Complexes (Fabaceae, Phaseoleae), Remy S. Pasquet, Thierry Vanderborght

All Peer-Reviewed Publications

An electrophoretic comparison of variation at 28 isozyme loci was performed for 58 Vigna accessions belonging to the V. luteola, V. ambacensis, and V. racemosa groups of species. In all three groups, strong divergence is noted between results and actual nomenclature.