Genetics and Genomics Commons^™

Cytologic Characterization Of Human Constitutive Heterochromatin, Timothy Atchison Donlon

Dissertations and Theses

A study was conducted to cytologically map certain subsets of constitutive heterochromatin onto specific portions of human chromosomes. This involved sequentially staining metaphase chromosomes from lymphocytes first with the Centromeric Dot, Giemsa-11, G-banding or Lateral Asymmetry staining techniques, which are cytochemical methods which stain particular chromosomes or chromosomal regions. Then those same metaphase chromosomes were stained using the C-banding technique, a method which is believed to denote constitutive heterochromatin. With the exception of the G-banding technique, areas depicted by the special staining techniques were found to reside only within the areas stained by the C-band technique and are thus believed …

Analysis Of Single Sperm Acrosomal Enzymes In Genetic Toxicology, Steven Craig Johnson

Masters Theses

No abstract provided.

The Association Of 5.8 S With 28s Ribosomal Rna, Nandita Banerjee

Chemistry & Biochemistry Theses & Dissertations

5.8S rRNA is a low molecular weight ribosomal RNA which is noncovalently bonded to the larger ribosomal subunit 28S rRNA; through its 3' end and through its 5' end. This interaction is an integral part of the ribosome, and plays an important role in the ribosome structure and function.

There is a high degree of homology between the 5.8S rRNA primary structures of rat, turtle and chicken. The base sequence of rat 5.8S rRNA differs only in one position from that of turtle and in three positions fr.om that of cl1.icken. Tl1ere is a single purine substitution at the 5' …

Genetic Heterogeneity In Complementation Groups Of Propionic Acidemia, Catherine Mckeon

Theses and Dissertations

Propionic acidemia is an autosomal recessively inherited disorder of organic acid metabolism caused by deficient activity of propionyl CoA carboxylase. This enzyme is required for the catabolism of the odd-chain fatty acids, the side chain of cholesterol and the amino acids: isoleucine, methionine, and threonine. Although the clinical expression of this disorder is variable, affected children usually develop ketoacidosis and hyperammonemia which may progress to seizures, coma and possibly death. Some patients may exhibit vomiting, lethargy and hypotonia in the first few weeks of life whereas others may remain asymptomatic for months or even years.(1) The degree of residual enzyme …